Article
Fisheries
Zhendong Xu, Zhiyang Huang, Junyao Zhong, Yinzhi Zhu, Xiaoyu Liu, Yongwei Wei
Summary: SCRV is a significant pathogen causing economic losses and serious threats to aquaculture. This study investigated the stability of SCRV in different environments and explored methods for its inactivation. SCRV showed high stability in the DMEM medium, surviving for 6 months at 4 degrees C and 1 year at 37 degrees C. The virus was also stable in ddH2O but weaker in pond water. On various surfaces, SCRV survived for up to 6 days at 25 degrees C. Heat inactivation at 55 degrees C for 30 minutes was not effective, but complete thermal inactivation could be achieved at 60 degrees C for 30 minutes or 65 degrees C for 5 minutes. UV irradiation for 120 seconds effectively inactivated SCRV. These findings are valuable for controlling SCRV outbreaks and have implications for disease control in fisheries.
Article
Fisheries
Xiaoyu Liu, Xiaodong Zhang, Zhendong Xu, Zhiyang Huang, Junyao Zhong, Yinzhi Zhu, Yongwei Wei
Summary: In this study, a reverse genetics system for Siniperca chuatsi rhabdovirus (SCRV) was established, allowing the recovery of infectious SCRV and verification of its similar phenotype compared to the parental virus. This system provides an important platform for the functional genome study and vaccine development of SCRV.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Xiaoyu Liu, Zhiyang Huang, Yupeng Miao, Pan Li, Yuehong Wang, Zhendong Xu, Xiaodong Zhang, Yongwei Wei
Summary: In this study, the EGFP gene was successfully inserted into the SCRV genome and a recombinant virus expressing EGFP was rescued. The recombinant virus had a high titer and stable expression of EGFP in EPC cells. In vitro and in vivo studies were conducted to investigate the growth characteristics, pathogenicity, and organ tropism of the recombinant virus. This study is of significant importance for understanding the pathogenesis of SCRV and the development of vaccines and antiviral drugs.
Article
Virology
Wenhui Liu, Jian He, Zhimin Li, Shaoping Weng, Changjun Guo, Jianguo He
Summary: This study investigated the molecular characterization, oxygen-sensing enzyme function, and role in virus infection of ADO in Mandarin fish. The results showed that scADO acted as an oxygen-sensing protein and responded to hypoxia and fish virus infection through the degradation of RGS4 protein and promotion of virus replication.
Article
Microbiology
Fei Ke, Xian-Yu Meng, Qi-Ya Zhang
Summary: This study revealed the main innate immune pathways in Siniperca chuatsi cells induced by Siniperca chuatsi rhabdovirus (SCRV) infection, as well as the major interferon-stimulated genes (ISGs) of Siniperca chuatsi in controlling SCRV replication.
Article
Biochemistry & Molecular Biology
Lan Ma, Ruixue Zhang, Demin Li, Tingting Qiao, Xiaoying Guo
Summary: Fluoride inhibits chondrocyte proliferation and promotes autophagy through the PI3K/AKT/mTOR signaling pathway in chondrogenesis. The study also showed that fluoride downregulates the mTOR signaling pathway-related genes and is associated with changes in autophagy-related gene expressions. MHY1485, a small-molecular mTOR activator, can reverse the fluoride-induced promotion of autophagy and recover the downregulation of proliferative chondrocyte markers induced by fluoride in chondrogenic cells.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Veterinary Sciences
Gianmarco Ferrara, Consiglia Longobardi, Sara Damiano, Roberto Ciarcia, Ugo Pagnini, Serena Montagnaro
Summary: This study aims to investigate the involvement of the PI3K/Akt/mTOR pathway during FeHV-1 infection. The results suggest that FeHV-1 may interact independently with different autophagic signaling pathways. Additionally, early phosphorylation of Akt after infection may be related to viral entry.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Cell Biology
Kexiang Zhang, Song Wu, Hongwei Wu, Li Liu, Jiahui Zhou
Summary: The study demonstrated that activation of Notch1 by doxycycline effectively suppressed cell proliferation and induced S phase arrest in osteosarcoma cells, while also triggering apoptosis and autophagy. Notch1 activation inhibited the PI3K/Akt/mTOR signaling pathway to induce apoptosis and autophagy.
Article
Plant Sciences
Pei-Pei Zhang, Bing-Yu Zhuo, Zi-Wei Duan, Song-Li Huang, Xin Li, Qian Cao, Ting Zhao, Sheng-Li Wei, Xiu-Hua Hub, Yuan Zhang
Summary: The study aimed to evaluate the lipid-lowering activity of Coreopsis tinctoria Nutt. (CT) through a mice model and a hepatoma cells model, and investigate its main components and mechanism. The study confirmed the lipid-lowering activity of CT extract and identified flavonoids as its main components. Among the main flavonoids, marein played a key role in promoting lipophagy by regulating the PI3K/AKT/mTOR pathway, resulting in a lipid-lowering effect.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kai Xu, Yuzhe He, Safwat Adel Abdo Moqbel, Xing Zhou, Lidong Wu, Jiapeng Bao
Summary: This study investigated the role of SIRT3 in osteoarthritis pathophysiology and found that SIRT3 serves as a protective agent against OA by inhibiting the PI3K/Akt/mTOR signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Medicine, Research & Experimental
Hui Gong, Xing Lyu, Yang Liu, Naling Peng, Shengyu Tan, Lini Dong, Xiangyu Zhang
Summary: Eupatilin alleviates pulmonary fibrosis through the Sestrin2/PI3K/Akt/mTOR-dependent autophagy pathway, reducing the expression of fibrotic markers. This study provides a new insight and potential drug target for the treatment of IPF.
Article
Biochemistry & Molecular Biology
Amir Barzegar Behrooz, Zahra Talaie, Fatemeh Jusheghani, Marek J. Los, Thomas Klonisch, Saeid Ghavami
Summary: Glioblastoma (GBM) is a devastating type of brain tumor, and targeting the Wnt/β-catenin and PI3K/Akt/mTOR (PAM) signaling pathways, which play crucial roles in GBM progression and invasion, may provide important advancements in alternative treatment strategies for GBM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Hui Li, Shufen Zhao, Liwei Shen, Peige Wang, Shihai Liu, Yingji Ma, Zhiwei Liang, Gongjun Wang, Jing Lv, Wensheng Qiu
Summary: The study revealed that high expression of E2F2 in gastric cancer is associated with poor prognosis, and it promotes cancer cell migration and invasion by inhibiting autophagy through the PI3K/Akt/mTOR pathway. E2F2 may also modulate tumor immunity.
Article
Biotechnology & Applied Microbiology
Yingnan Dai, Yeping Chen, Guoqian Wei, Li Zha, Xueqi Li
Summary: This study demonstrated that Ivabradine attenuated myocardial infarction in rats by enhancing autophagy, and the PI3K/AKT/mTOR/p70S6K pathway might be involved in this process. Activation of autophagy by Ivabradine could be a potential therapeutic strategy for the treatment of myocardial infarction.
Article
Chemistry, Medicinal
Ai-Yuan Xiu, Qian Ding, Zhen Li, Chun-Qing Zhang
Summary: Doxazosin inhibits autophagy by activating the PI3K/Akt/mTOR signaling pathway, attenuating liver fibrosis. It also blocks HSC proliferation and migration, inducing cell apoptosis in a concentration-dependent manner. Additionally, doxazosin activates the PI3K/Akt/mTOR pathway which regulates fibrosis, autophagy, and apoptosis in HSCs.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)