期刊
EUROPEAN JOURNAL OF SOIL SCIENCE
卷 72, 期 2, 页码 1026-1041出版社
WILEY
DOI: 10.1111/ejss.13026
关键词
archaea; bacteria; environmental DNA; fungi; long-term field experiment; soil organic matter
类别
Despite technological advances, DNA extraction from soil is crucial for studying soil microbial communities. Different methods can significantly affect fungal and archaeal communities compared to bacterial ones. The choice of DNA extraction method can impact the correlations between microbial communities and environmental variables.
Despite recent technological advances in molecular ecology, DNA extraction from soil remains a crucial step when quantifying and characterizing soil microbial communities. Potential biases could hamper fundamental understanding of the dynamic relationships between soil properties and microorganisms under different agricultural practices. In this study, we compared four different DNA extraction methods for their ability to discriminate microbial communities of an arable soil subjected to different fertilization managements for more than 50 years (Ultuna, Sweden). The abundance and the diversity of bacteria, archaea and fungi were studied using qPCR and molecular fingerprints, respectively. Overall, the choice of DNA extraction method had a more pronounced effect on the fungal and archaeal communities in comparison to bacterial ones. The assessment of the microbial diversity was more sensitive to DNA extraction methods in comparison with the quantification of the abundances. The DNA extraction method clearly affects the intensity of the correlations between the abundance and/or diversity of microbial communities and environmental variables (C, N and pH) according to the targeted taxon. This study highlights that long-term effects can be offset by biases in DNA extraction methods. Highlights Using different DNA extraction methods may alter soil microbial survey. Average DNA concentrations vary from 1 to 3 mu g.g(-1)according to the extraction method. Gene copies numbers vary up to 2 orders of magnitude among DNA extraction methods. Fungal communities diversity descriptors were the most affected by DNA extraction
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