Article
Environmental Sciences
Weidong Zhang, Jiachao Wang, Jianshi Song, Yanru Feng, Shujuan Zhang, Na Wang, Shufeng Liu, Zhixue Song, Kaoqi Lian, Weijun Kang
Summary: This study evaluated the effects of residual low-concentrations of Glyphosate (GLY) and its degradation product AMPA on zebrafish embryos, finding developmental toxicity including increased heart rates, arrhythmia, cardiac defects, and altered gene transcription levels. Pericardial edema and bone deformation may be caused by inhibition of specific enzymes after exposure to GLY and AMPA. The results suggest that typical environmental residual concentrations of GLY and AMPA have similar developmental toxicity in zebrafish embryos.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Zhihui Liu, Yingying Shangguan, Penglin Zhu, Yousef Sultan, Yiyi Feng, Xiaoyu Li, Junguo Ma
Summary: Glyphosate exposure in zebrafish larvae induces developmental toxicity, characterized by premature hatching, reduced heartbeats, and body deformities. This is likely due to disrupted thyroid function and abnormal expression of growth hormone/insulin-like growth factor axis-related genes. Additionally, glyphosate treatment leads to oxidative damage, endoplasmic reticulum stress, inflammatory reactions, and apoptosis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Jian Lu, Weiguo Wang, Cheng Zhang, Wenping Xu, Weidong Chen, Liming Tao, Zhong Li, Jiagao Cheng, Yang Zhang
Summary: Glyphosate, the most widely used herbicide, poses new hazards to human health, particularly cardiovascular toxicity. Research on zebrafish embryos exposed to glyphosate showed developmental abnormalities, including mortality, hatching issues, body length reduction, cardiac malformations, and impaired angiogenesis. Mechanistically, apoptosis occurred in the heart and vascular regions, with altered expression levels of apoptosis-related genes. This study provides evidence linking glyphosate exposure to cardiovascular developmental toxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Mengying Yang, Jinyu Qiu, Xin Zhao, XiZeng Feng
Summary: The research results demonstrated that 6-BA had adverse effects on the development and neurobehavior of zebrafish, including developmental delay, decreased survival and hatchability, accumulation of oxidative stress, as well as impaired locomotion and sleep/wake behavior.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Ekrem Sulukan, Alper Baran, Meryem Kankaynar, Tugba Kiziltan, Ismail Bolat, Serkan Yildirim, Hacer Akguel Ceyhun, Saltuk Bugrahan Ceyhun
Summary: The increase in temperature due to global warming affects the toxicity of pesticides in the aquatic ecosystem, particularly in terms of the sustainability of ecosystems. This study investigated the effects of parental exposure to glyphosate and temperature increase on the next generation using a zebrafish model. The results showed that the offspring exhibited lower survival rate, delayed hatching, increased body malformations, and lower blood flow and heart rate.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Environmental Sciences
Germano A. B. Lanzarin, Luis M. Felix, Antonio Fontainhas-Fernandes, Sandra Mariza Monteiro, Carlos Venancio
Summary: A critical review was conducted to investigate the toxicity of glyphosate and glyphosate-based herbicides on zebrafish. The results showed that both substances can cause damage to zebrafish, compromising their survival, reproduction, and maintenance. These findings may be applicable to other fish species and aquatic ecosystems.
Article
Environmental Sciences
Keyuan Zhong, Yunlong Meng, Juan Wu, You Wei, Yong Huang, Jinze Ma, Huiqiang Lu
Summary: The study revealed that flupyradifurone has toxic effects on zebrafish embryos, leading to decreased survival rate, heart rate, and body length, as well as abnormal heart development and increased oxidative stress levels. Moreover, flupyradifurone also affects the expression of genes related to apoptosis and heart development. The findings provide new evidence on the toxic effects of flupyradifurone and the potential risks associated with its residues in agricultural products and the environment.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Gabriela Beatriz Alvarado-Suarez, Marcelo Silva-Briano, Mario Alberto Arzate-Cardenas, Ana Laura Carbajal-Hernandez, Beatriz Yanez-Rivera, Roberto Rico-Martinez
Summary: Glyphosate levels and transfer across trophic levels in zooplankton have been rarely studied. It was found that glyphosate was more likely to be incorporated through the medium than through food (zooplankton). The intake of glyphosate led to decreased survival and feeding rates in zebrafish larvae, while zooplankton species bioconcentrated glyphosate in large quantities.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Dan Li, Weijun Sun, Hongxing Chen, Haojun Lei, Xiao Li, Hongsong Liu, Guo-yong Huang, Wen-jun Shi, Guang-Guo Ying, Yongju Luo, Lingtian Xie
Summary: The study investigated the toxicity of cyclophosphamide on zebrafish embryos, revealing the effects on malformation, mortality, swimming capacity, retina and liver tissues, and key gene expressions. Low concentrations of cyclophosphamide pose ecological risks to aquatic organisms, highlighting the need for further research.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Christelle Simasotchi, Audrey Chissey, Gerald Jungers, Thierry Fournier, Gilles-Eric Seralini, Sophie Gil
Summary: Glyphosate-based herbicidal formulations, such as Roundup, consist of glyphosate as the active principle and other undisclosed formulants. Studies show that these formulants are more toxic than glyphosate, affecting placental permeability and fetal blood flow. Formulants may disrupt fetal-placental circulation and integrity, potentially leading to adverse pregnancy outcomes.
Article
Environmental Sciences
Yuanzhao Wu, Jiawen Wang, Yumei Xia, Kaiqin Tang, Jincheng Xu, Anli Wang, Shundi Hu, Luhong Wen, Binjie Wang, Weixuan Yao, Jiye Wang
Summary: This study demonstrated the developmental and neurotoxic effects of Isofenphos-methyl (IFP) on zebrafish embryos. IFP exposure resulted in abnormal heart development, uninflated swim bladder, and behavioral defects. Furthermore, IFP exposure activated oxidative stress and reduced acetylcholinesterase (AChE) content.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Luis Terrazas-Salgado, Beatriz Yanez-Rivera, Raul Llera-Herrera, Alejandra Garcia-Gasca, Isabel Alvarado-Cruz, Miguel Betancourt-Lozano
Summary: This study aimed to evaluate the effects of glyphosate at environmental concentrations on zebrafish embryos. The results showed that exposure to high concentrations of glyphosate may lead to a decrease in heart rate in zebrafish embryos. In addition, transcriptomic analysis revealed subtle changes in gene expression profiles, including genes related to oocyte maturation, metabolic processes, histone deacetylation, and nervous system development.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2022)
Article
Environmental Sciences
Peng Xiao, Wenhua Li, Jinfang Lu, Yang Liu, Qiulan Luo, He Zhang
Summary: The study found that Bixafen has toxic effects on the retinal development of zebrafish embryos, leading to severe retinal defects and increased cell density in the ganglion cell layer.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Marianna Marino, Elena Mele, Andrea Viggiano, Stefania Lucia Nori, Rosaria Meccariello, Antonietta Santoro
Summary: Glyphosate is a widely used herbicide that has long been considered to have no or weak toxicity in humans, but increasing evidence suggests its potential toxicity in animals and human cells and tissues. Therefore, some countries have started taking precautionary measures to reduce its spread.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Dongjie Wang, Xinyi Leng, Yao Tian, Jiangdong Liu, Jixing Zou, Shaolin Xie
Summary: Koumine, a common alkaloid found in Gelsemium elegans, has various pharmacological effects such as anti-tumor and anti-inflammatory properties. However, its high toxicity and unclear mechanism of action have limited its medicinal use. This study investigated the toxic effects of koumine on zebrafish embryos and larvae, and found that it affected their development and behavior by influencing the ACh content and AChE activity. The safe concentration of koumine for zebrafish was found to be less than 25 mg/L.
Article
Environmental Sciences
Luis Felix, Paulo Carreira, Francisco Peixoto
Summary: This study investigates the biological effects of naturally weathered microplastics (MPs) on zebrafish, revealing anxiety-like behavior and mitochondrial dysfunction caused by the MPs. It also emphasizes the potential cumulative impact of these particles on aquatic ecosystems.
Review
Fisheries
Luis Felix, Raquel Vieira, Sandra M. Monteiro, Carlos Venancio
Summary: The use of anaesthetic agents, including natural substances such as clove oil, as alternatives to the commonly used synthetic anaesthetic MS-222, has been investigated for fish anaesthesia. A meta-analysis of 30 English reports found that 10 different monoterpenes showed high effectiveness in inducing anaesthesia and recovery in fish. The findings also revealed an inverse linear association between anaesthesia induction and recovery time and water bath temperature.
FISH AND FISHERIES
(2023)
Article
Biochemistry & Molecular Biology
Francisco Pereira, Adriana Pereira, Sandra M. Monteiro, Carlos Venancio, Luis Felix
Summary: This study aimed to evaluate the antioxidant effects of a natural plant hormone, 24-epibrassinolide, against the teratogenic effects of nicotine on zebrafish embryos. The results showed that 24-epibrassinolide reduced nicotine-induced deformities in zebrafish embryos, while improving oxidative stress and metabolic changes. It also alleviated nicotine-induced cognitive dysfunction.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Food Science & Technology
Manyou Yu, Irene Gouvinhas, Maria J. Pires, Maria J. Neuparth, Rui M. Gil da Costa, Rui Medeiros, Margarida M. S. M. Bastos, Helena Vala, Luis Felix, Carlos Venancio, Ana I. R. N. A. Barros, Paula A. Oliveira
Summary: A study found that pomegranate leaf infusion has anti-neoplastic and toxicological properties in transgenic mice carrying HPV16 oncogenes. It can reduce lesions in the liver, kidney, and skin. This suggests that pomegranate leaf infusion may have potential in preventing and treating HPV16-induced lesions, but further research is needed for confirmation.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Review
Environmental Sciences
Germano A. B. Lanzarin, Luis M. Felix, Antonio Fontainhas-Fernandes, Sandra Mariza Monteiro, Carlos Venancio
Summary: A critical review was conducted to investigate the toxicity of glyphosate and glyphosate-based herbicides on zebrafish. The results showed that both substances can cause damage to zebrafish, compromising their survival, reproduction, and maintenance. These findings may be applicable to other fish species and aquatic ecosystems.
Article
Agriculture, Dairy & Animal Science
Sara Jorge, Luis Felix, Benjamin Costas, Ana M. M. Valentim
Summary: Proper housing conditions are important for fish welfare. This study found that environmentally enriched housing conditions had minor effects on zebrafish behavior, but no significant influence on body length and stress hormone levels. It suggests that housing conditions should be considered in future research.
Article
Biochemistry & Molecular Biology
Germano A. B. Lanzarin, Luis M. Felix, Sandra M. Monteiro, Jorge M. Ferreira, Paula A. Oliveira, Carlos Venancio
Summary: This study investigated the anti-inflammatory, antioxidant, and anti-apoptotic effects of the plant-based products Thymol (THY) and 24-epibrassinolide (24-EPI). Using zebrafish larvae as a model, the researchers found that both compounds reduced neutrophil recruitment, exhibited antioxidant effects by reducing reactive oxygen species (ROS) production, and displayed anti-apoptotic effects. Furthermore, the compounds also decreased the levels of nitric oxide (NO). These findings suggest that THY and 24-EPI have potential as anti-inflammatory and antioxidant agents.
Article
Agriculture, Dairy & Animal Science
Mariana Caipira Lei, Luis Felix, Ricardo Cardoso, Sandra Mariza Monteiro, Severiano Silva, Carlos Venancio
Summary: This study investigates the use of saliva and eye infrared thermography as non-invasive methods to detect transport-induced stress in calves. The results show that transport increases salivary cortisol, oxidative stress parameters, and eye temperatures, indicating that saliva and infrared thermography are effective in assessing cows' stress status.