Article
Genetics & Heredity
Lijun Yan, Yusheng Ou, Shengfang Xia, Jianqing Huang, Wenfeng Zhang, Hongwei Shao, Han Shen, Huaben Bo, Changli Tao, Jinquan Wang, Fenglin Wu
Summary: Effector T cells with overexpression of early characteristic transcription factors show enhanced anti-tumor immunity through dedifferentiation, with the S1 group demonstrating the most effective dedifferentiation induction.
Article
Immunology
Ryan Zander, Moujtaba Y. Kasmani, Yao Chen, Paytsar Topchyan, Jian Shen, Shikan Zheng, Robert Burns, Jennifer Ingram, Can Cui, Nikhil Joshi, Joseph Craft, Allan Zajac, Weiguo Cui
Summary: This study found that CD4(+) T cells consist of three distinct subsets during chronic viral infection, and IL-21 derived from Tfh cells is critical for sustaining CD8(+) T cell responses and viral control.
Article
Oncology
Zhiqiang Ren, Yinyin Xue, Liang Liu, Xuesai Zhang, Jinpeng Pei, Yu Zhang, Yue Wang, Ker Yu
Summary: In this study, abnormal expression of tissue factor (TF) was found to be associated with poor prognosis and low immune effector cell infiltration in triple-negative breast cancer (TNBC). Inhibition of TF in tumor cells was shown to inhibit tumor growth and increase infiltration of effector T cells. Furthermore, a fusion protein targeting both TF and TGFβ showed enhanced anti-tumor effects and improved tumor immune environment. The study also revealed a novel mechanism of TF in TNBC, which involves blocking the synthesis and secretion of chemokines CXCL9/10/11 to inhibit T cell recruitment and function.
Article
Multidisciplinary Sciences
Shilu Zhang, Saptarshi Pyne, Stefan Pietrzak, Spencer Halberg, Sunnie Grace McCalla, Alireza Fotuhi Siahpirani, Rupa Sridharan, Sushmita Roy
Summary: The authors developed a method called single-cell Multi-Task Network Inference (scMTNI) to accurately infer the dynamics of cell type-specific Gene Regulatory Networks (GRNs) from scRNA-seq and scATAC-seq datasets. This method can identify key regulators and accurately model network dynamics for diverse processes such as cellular reprogramming and differentiation.
NATURE COMMUNICATIONS
(2023)
Article
Peripheral Vascular Disease
Akiko Hiramatsu, Yuichiro Izumi, Koji Eguchi, Naomi Matsuo, Qinyuan Deng, Hideki Inoue, Yushi Nakayama, Hiroshi Nonoguchi, Jose Aramburu, Cristina Lopez-Rodriguez, Yutaka Kakizoe, Masataka Adachi, Takashige Kuwabara, Shokei Kim-Mitsuyama, Masashi Mukoyama
Summary: The study showed that renal tubular NFAT5 plays an important role in regulating sodium reabsorption through the epithelial sodium channel under high-salt conditions, thereby preventing salt-dependent hypertension.
Article
Cell Biology
Qicai Han, Penghui Yan, Ruipeng Song, Feifei Liu, Qing Tian
Summary: The upregulation of mitochondrial protein TIMM13 in human osteosarcoma tissues and cells is essential for mitochondrial integrity and function. Depletion of TIMM13 leads to mitochondrial dysfunction and injury, inhibits cell proliferation and migration, and promotes apoptosis in osteosarcoma cells. TIMM13 represents a novel therapeutic target for osteosarcoma cell growth.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Su Yin Lim, Sara Alavi, Zizhen Ming, Elena Shklovskaya, Carina Fung, Ashleigh Stewart, Helen Rizos
Summary: Our study found that tumor necrosis factor alpha (TNF alpha) can influence the differentiation status and immune inhibition of melanoma cells, with different impacts on different melanoma cells. TNF alpha showed poor induction of antigen presentation molecules but readily induced the PD-L2 immune checkpoint in melanoma cells. Our results suggest that TNF alpha promotes dynamic changes in melanoma cells that may favor immunotherapy resistance.
Article
Multidisciplinary Sciences
Siqian Feng, Richard S. Mann
Summary: Chromatin immunoprecipitation (ChIP) is a crucial technique for studying protein-DNA interactions, but lacks cell type-specificity in complex tissues. SpyChIP overcomes this limitation by utilizing a specific isopeptide bond to identify cell type-specific transcription factor (TF) binding sites, offering insights into TF mechanisms in different cell types.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Han Feng, Zixuan Zhao, Xiaohong Zhao, Xue Bai, Weiwei Fu, Liangtao Zheng, Boxi Kang, Xiaohu Wang, Zemin Zhang, Chen Dong
Summary: Using single-cell transcriptomic analysis, researchers identified a novel T follicular helper cell subset with stem cell-like transcriptional features and long-term survival ability, which can give rise to effector Tfh cells in recall immune responses.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Immunology
Julia Campe, Evelyn Ullrich
Summary: Allogenic hematopoietic stem cell transplantation is a potent treatment for hematologic disorders, but can also lead to graft-versus-host disease. CD4 T cell subtypes and transcription factor inhibitors play an important role in the pathogenesis and regulation of acute graft-versus-host disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Hwee Hui Lau, Nicole A. J. Krentz, Fernando Abaitua, Marta Perez-Alcantara, Jun-Wei Chan, Jila Ajeian, Soumita Ghosh, Yunkyeong Lee, Jing Yang, Swaraj Thaman, Benoite Champon, Han Sun, Alokkumar Jha, Shawn Hoon, Nguan Soon Tan, Daphne Su-Lyn Gardner, Shih Ling Kao, E. Shyong Tai, Anna L. Gloyn, Adrian Kee Keong Teo
Summary: The coding variant of the PAX4 gene is associated with an altered risk of type 2 diabetes in East Asian populations. This study found that individuals carrying the specific allele of PAX4 exhibited decreased pancreatic beta cell function, and a newly identified allele was found in some diabetes patient pedigrees. Knockdown of the PAX4 gene resulted in impaired insulin secretion and altered hormone gene expression in a human beta cell model. In vitro differentiation experiments also showed that cells carrying specific risk alleles exhibited increased polyhormonal endocrine cell formation and decreased insulin content.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Luis Almeida, Ayesha Dhillon-LaBrooy, Carla N. Castro, Nigatu Adossa, Guilhermina M. Carriche, Melanie Guderian, Saskia Lippens, Sven Dennerlein, Christina Hesse, Bart N. Lambrecht, Luciana Berod, Leif Schauser, Bruce R. Blazar, Markus Kalesse, Rolf Mueller, Luis F. Moita, Tim Sparwasser
Summary: Linezolid and other ribosomal-targeting antibiotics effectively inhibit T cell activity, disrupt mitochondrial function, reduce oxidative phosphorylation levels, and prevent the occurrence of autoimmune diseases.
Article
Multidisciplinary Sciences
Vanessa Zaiatz-Bittencourt, Fiona Jones, Miriam Tosetto, Caitriona Scaife, Gerard Cagney, Evan Jones, Glen A. Doherty, Elizabeth J. Ryan
Summary: The gut microbiota plays a crucial role in regulating chronic inflammation and its involvement in the development of autoimmune diseases and cancer. Microbial short-chain fatty acids, such as butyrate, have immunomodulatory effects and are believed to mediate the host-microbiome interaction. This study demonstrates that butyrate has a strong anti-inflammatory effect on NK cells and restricts their function through downregulation of mTORC1 activity, c-Myc mRNA expression, and metabolism.
SCIENTIFIC REPORTS
(2023)
Article
Endocrinology & Metabolism
Martha Elena Diaz-Hernandez, Christopher W. Kinter, Shana R. Watson, Giovanni Mella-Velazquez, Jarred Kaiser, Guanglu Liu, Nazir M. Khan, Joseph L. Roberts, Joseph Lorenzo, Hicham Drissi
Summary: This study explores the effects of Runx1, a transcription factor, on bone resorption and osteoclastogenesis. The researchers found that Runx1 acts as an inhibitor of osteoclast formation and its overexpression delays osteoclastogenesis and decreases bone resorption in a sex-dependent manner. Transcriptomics analysis revealed that Runx1 overexpression leads to a distinct gene expression profile related to redox signaling, apoptosis, osteoclast differentiation, and bone remodeling.
Article
Immunology
Bryan McDonald, Brent Y. Chick, Nasiha S. Ahmed, Mannix Burns, Shixin Ma, Eduardo Casillas, Dan Chen, Thomas H. Mann, Carolyn O'Connor, Nasun Hah, Diana C. Hargreaves, Susan M. Kaech
Summary: CD8+ T cells differentiate into distinct effector and memory cell subsets to protect the host against pathogens. The role of the canonical BAF chromatin remodeling complex in antiviral CD8+ T cells during infection was investigated. ARID1A, a subunit of cBAF, establishes open chromatin regions at enhancers, which is critical for TF binding, proliferation, gene expression, and effector and memory differentiation. Arid1a deficiency impairs the opening of activation-induced enhancers and tissue-resident memory formation.
Article
Oncology
Chuang Qu, Shuhui Gao, Hongwei Shao, Wenfeng Zhang, Huabben Bo, Xin Lu, Tianjiao Chen, Jing Kou, Yue Wang, Gui Si Chen, Shulin Huang, Han Shen
Article
Oncology
Lan Huang, Guisi Chen, Ying Chen, Wanwen Wu, Changli Tao, Hongwei Shao, Shulin Huang, Han Shen
Summary: Tumor immunotherapy targeting tumor-associated antigens and the impact of GM-CSF and CCL17 on tumor immunity have been widely studied. Combining survivin-specific CTL with CCL17 and GM-CSF genes can inhibit tumor growth more effectively through stable cell lines expressing these genes.
Article
Biotechnology & Applied Microbiology
C. Tao, W. Zeng, Q. Zhang, G. Liu, F. Wu, H. Shen, W. Zhang, H. Bo, H. Shao
Summary: ITF supplementation delayed the recovery of antibiotic-induced colitis, did not fully restore the microbial community composition, increased beneficial bacteria and butyrate levels, but led to selective blooms of opportunistic pathogens.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Oncology
Wanwen Wu, Ying Chen, Lan Huang, Wenjian Li, Changli Tao, Han Shen
Article
Medicine, Research & Experimental
Hui Wang, Hua Lu, Ying-shou Lei, Chen-yu Gong, Zhao Chen, Ying-qiao Luan, Qiang Li, Ying-zhen Jian, Hao-zheng Wang, Feng-lin Wu, Chang-li Tao, Han Shen, Hua-ben Bo, Hong-wei Shao, Wen-feng Zhang
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2020)
Review
Cell Biology
Ying Chen, Lan Huang, Yongjian Dong, Changli Tao, Rongxin Zhang, Hongwei Shao, Han Shen
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Oncology
Yongjian Dong, Shuhui Gao, Xuefang Zhang, Jing Kou, Jing Liu, Ting Ye, Han Shen
Summary: Recent studies have shown that dendritic cell-activated CIK (DC-CIK) cells exhibit enhanced antitumor activity compared to unstimulated CIK cells. It has been found that dendritic cells can secrete CCL17 and CCL22 to increase the expression of CCR4 on CIK cells, thereby enhancing their migratory capacity and tumor-killing efficacy.
Article
Genetics & Heredity
Lijun Yan, Yusheng Ou, Shengfang Xia, Jianqing Huang, Wenfeng Zhang, Hongwei Shao, Han Shen, Huaben Bo, Changli Tao, Jinquan Wang, Fenglin Wu
Summary: Effector T cells with overexpression of early characteristic transcription factors show enhanced anti-tumor immunity through dedifferentiation, with the S1 group demonstrating the most effective dedifferentiation induction.
Article
Biotechnology & Applied Microbiology
Wenjing Zeng, Qin Zhang, Gang Feng, Gongliang Liu, Fenglin Wu, Han Shen, Hongwei Shao, Changli Tao
Summary: This study evaluated the effect of supplementation with inulin-type fructans (ITFs) on intestinal immune function in mice with dysbiosis induced by antibiotic treatment. The results showed that ITF supplementation restored the composition of the intestinal microbial community, but delayed inflammation recovery in the intestine and had regulatory effects on immune cells and inflammatory genes.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Ting Ye, Xuefang Zhang, Yongjian Dong, Jing Liu, Wenfeng Zhang, Fenglin Wu, Huaben Bo, Hongwei Shao, Rongxin Zhang, Han Shen
Summary: High expression of CCL17 in the tumor microenvironment (TME) promotes better survival and prognosis for early-stage lung adenocarcinoma (LUAD) patients, correlating with tumor-infiltrating lymphocytes, immunostimulators, and tumor mutation burden, while negatively correlated with immune score.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Qiurui Chen, Xuejia Wang, Yichen Zheng, Ting Ye, Jing Liu, Jin-Quan Wang, Wen-feng Zhang, Feng-Lin Wu, Huaben Bo, Hongwei Shao, Rongxin Zhang, Han Shen
Summary: This study revealed the relationship between the abundance of cancer-associated fibroblasts (CAFs) and tumor microenvironment characteristics, prognosis, and treatment response in hepatocellular carcinoma (HCC). It suggests that the combination therapy of PD-1 inhibitors and anti-angiogenic drugs may be more valuable for patients with high CAF abundance.
Article
Biochemistry & Molecular Biology
Biaojie Huang, Qiurui Chen, Zhiyun Ye, Lin Zeng, Cuibing Huang, Yuting Xie, Rongxin Zhang, Han Shen
Summary: This study identified four distinct CAF subtypes of breast cancer using single-cell RNA sequencing data. Matrix CAFs were strongly associated with tumor matrix remodeling and the TGF-fi signaling pathway. Patients in the high-fibrotic group had significantly poor prognosis. A risk prognosis signature (mRPS) was developed and showed higher predictive accuracy than the conventional TNM staging system. mRPS was correlated with the infiltration level of anti-tumor immune cells. Based on consensus prognostic genes, breast cancer samples were classified into two immune subtypes, IFN-gamma-dominant and TGF-beta-dominant. Patients with mRPS downregulation had improved prognosis and could potentially benefit from immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mathematical & Computational Biology
Jing Liu, Xuefang Zhang, Ting Ye, Yongjian Dong, Wenfeng Zhang, Fenglin Wu, Huaben Bo, Hongwei Shao, Rongxin Zhang, Han Shen
Summary: A prognostic model based on the tumor immune microenvironment characteristics was established for metastatic melanoma. Immune infiltration and immune checkpoint analysis revealed differences between high and low risk groups.
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2022)
Article
Oncology
Wanwen Wu, Ying Chen, Lan Huang, Wenjian Li, Changli Tao, Han Shen
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2020)
Article
Cell Biology
Jeffrey Maslanka, Gretel Torres, Jennifer Londregan, Naomi Goldman, Daniel Silberman, John Somerville, James E. Riggs
Summary: This study investigates the immunobiology of the peritoneum in ovarian cancer, revealing reduced B1 cells in the ascites and selective loss of B1 and marginal zone B cell subsets in the spleen. These findings suggest a correlation between the depletion of B cell subsets and the influx of myeloid-derived suppressor cells during ovarian cancer.
CELLULAR IMMUNOLOGY
(2024)