Article
Energy & Fuels
Timo Steinbrecher, Fabian Bonk, Marvin Scherzinger, Oliver Luedtke, Martin Kaltschmitt
Summary: This study investigates the fractionation of fibrous straw digestate through combined hydrothermal and enzymatic treatment and the influence of impurities in this process. The findings show that around 50% of hemicellulose is solubilized after hydrothermal treatment, and around 90% and 92% of cellulose and hemicellulose are hydrolyzed through enzymatic treatment. Lignin accumulates in the remaining solid but undergoes structural degradation during hydrothermal treatment.
Article
Chemistry, Applied
Gabriel Banvillet, Gael Depres, Naceur Belgacem, Julien Bras
Summary: Efficient production of cellulose nanofibrils from eucalyptus fibers was achieved using a combination of NaOH and enzymatic treatments followed by pilot scale grinding. The treatment resulted in CNF with a rigid structure, increased crystallinity indices, and reduced DP values, with a multi-scale analysis of properties and energy consumption. Mechanical properties of nanopapers decreased but optical properties improved, showing potential for industrial applications with reduced energy needs.
CARBOHYDRATE POLYMERS
(2021)
Article
Food Science & Technology
Wenhan Zhang, Nan Jia, Zihao Zhu, Yanchao Wang, Jingfeng Wang, Changhu Xue
Summary: This study investigated the effects of enzymatic hydrolysis and activated carbon decoloration on antioxidative activities and peptidomic patterns of microalgae protein hydrolysates using a peptidomic approach. Activated carbon decoloration significantly increased color lightness but decreased antioxidative activities and relative abundances of peptides, particularly aromatic amino acid containing peptides. Understanding the dynamics of production processes at a molecular scale can enhance the production and quality control of bioactive peptides from Auxenochlorella pyrenoidosa.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Chemical
Shuli Liu, Camille Rouquie, Laurence Lavenant, Matthieu Frappart, Estelle Couallier
Summary: Microalgae biorefinery is an efficient method to extract intracellular compounds from microalgae, such as lipids, proteins, polysaccharides, and pigments. This study focuses on the recovery of lipids and hydrosoluble compounds from Parachlorella kessleri under starving conditions. The impact of different operational conditions on compound recovery and filtration performance was evaluated. The best scenario achieved a recovery of 23% lipids, 9% sugars, and 8% proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Yingji Wu, Shengbo Ge, Changlei Xia, Changtong Mei, Ki-Hyun Kim, Liping Cai, Lee M. Smith, Jechan Lee, Sheldon Q. Shi
Summary: Intermittent ball milling was effective in enhancing enzymatic hydrolysis of dilute acid-pretreated lignocellulosic biomass, leading to higher glucose yield compared to typical hydrolysis. This technology shows promise for more efficient and environmentally friendly approaches to isolating glucose from lignocellulosic biomass.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2021)
Article
Engineering, Chemical
Rashi Vishwakarma, Anushree Malik
Summary: A green extraction process is proposed to reduce energy and petroleum solvent consumption, while maintaining environmental safety and quality. The study focuses on generating a highly permeable cell using crude fungal enzymes, enabling simultaneous cultivation and extraction. A novel pretreatment method combining partial enzymatic digestion and maintaining viability of microalgae cells allows for further product recovery from the same biomass.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jinzhi Yang, Di Cai, Xudong Liu, Liqi Zhu, Changwei Zhang, Qing Peng, Yanxia Han, Guozhen Liu, Ming Yang
Summary: This study compared the enzymatic hydrolysis efficiency of wet, freeze-dried, and oven-dried microalgal biomass, with and without an acidic pretreatment. The results showed that wet microalgal biomass had a higher glucose yield compared to oven-dried biomass, possibly due to the hindered accessibility of enzymes in the cross-linked cells of the latter. Acidic pretreatment significantly improved the glucose recovery, with potential for direct saccharification process. The enzymatic hydrolysate of wet microalgal biomass was successfully used for ABE fermentation, producing 7.2 g/L of ABE, indicating its potential in bioalcohol fuel fermentation.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Kaili Ding, Hao Lin, Luoyang Liu, Xiwen Jia, Hui Zhang, Yufeng Tan, Xueyan Liang, Yinghui He, Dong Liu, Lujia Han, Weihua Xiao
Summary: In this study, corn stover was analyzed and divided into different parts. It was found that the husk obtained the highest monosaccharide concentration after ball milling pretreatment, due to its improved structural properties. Ball milling pretreatment enhanced the crystallinity, xylan content, and water retention value, leading to increased sugar yield.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Hugo Mear, Pascale Gillon, Imma Gifuni, Laurence Lavenant, Alexia Poidevin, Estelle Couallier
Summary: Tetraselmis is a promising microalga with high protein content and productivity, although it is less studied compared to other algae. Two batches of Tetraselmis were received, showing different morphological properties possibly due to differences in physiological state. Biochemical characterization and cell disruption tests revealed significant differences between the two batches, affecting protein recovery yields. Various physicochemical modifications were applied to increase yields, with dilution being the most promising. Understanding the physiological state of Tetraselmis is crucial for optimizing biorefinery processes and maximizing protein recovery yields.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Physical
Cheng Li, Li Wang, Zhengxing Chen, Yongfu Li, Xiaohu Luo, Feiyang Zhao
Summary: By studying different pretreatment methods for wheat bran, it was found that sequential combination of ozonolysis and subcritical water showed a synergistic effect, significantly improving the enzymatic digestion and hemicellulose solubilization of wheat bran, providing new possibilities for wheat bran biorefinery.
JOURNAL OF SUPERCRITICAL FLUIDS
(2021)
Article
Agricultural Engineering
Wenjun Ying, Xin Fang, Yong Xu, Junhua Zhang
Summary: Efficient production of xylooligosaccharides (XOS) from poplar was achieved by combining acetic acid (AC) hydrolysis and hydrogen peroxide-acetic acid (HPAC) pretreatment, resulting in a significant increase in XOS yield compared to xylanase hydrolysis alone.
BIOMASS & BIOENERGY
(2022)
Article
Thermodynamics
Hanieh Shokrkar, Amin Keighobadi
Summary: The study investigated the effect of various fluid hydrodynamic conditions on the enzymatic hydrolysis of microalgae for glucose extraction. It was found that understanding the mixing effects in enzymatic hydrolysis could improve the economic feasibility of the process, and that stirring speed and baffle presence significantly influenced glucose extraction efficiency. The results also showed that altering impeller type had a negligible effect on enzymatic hydrolysis rate.
Article
Food Science & Technology
Pedro Ferreira-Santos, Silvia M. Miranda, Isabel Belo, Giorgia Spigno, Jose A. Teixeira, Cristina M. R. Rocha
Summary: A sequential multi-stage procedure was used to extract biocompounds from Spirulina platensis, with the combination of ohmic heating and enzymes allowing for high yield and high purity extraction. The intensified extraction steps facilitate the use of environmentally friendly technologies, targeting waste reduction and contributing to circular economy strategies.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Food Science & Technology
Johanna F. Alberts, Ibtisaam Davids, Wulf-Dieter Moll, Gerd Schatzmayr, Hester-Mari Burger, Gordon S. Shephard, Wentzel C. A. Gelderblom
Summary: The study evaluated the detoxification of total fumonisin B mycotoxins in commercial maize using FumD, which effectively reduced the FB1 content and significantly decreased the levels of fumonisins in total hominy feed. The introduction of FumD shows potential for reducing exposure risk to FB1 in maize-based food products.
Article
Agricultural Engineering
Qianqian Yang, Lei Zhang, Zhina Lian, Junhua Zhang
Summary: In this study, lactic acid was used for XOS production from corncob, achieving a XOS yield of 69.9%. Additionally, cellulase hydrolysis of corncob residue resulted in 95.6% glucose and 54.0% xylose. Bacillus subtilis YS01 was successfully cultured using the cellulase hydrolysate, with a viable count of 6.4x10(8) CFU/mL and glucose and xylose utilization rates of 99.0% and 89.8%, respectively.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Sarah D'Adamo, Richard Kormelink, Dirk Martens, Maria J. Barbosa, Rene H. Wijffels
Summary: Viruses, besides being pathogens, play a crucial role in evolution and can influence host fitness, population dynamics, and community structures. Microalgae biotechnology is a rapidly advancing field that investigates the use of photosynthetic microorganisms for various industries. The study of viruses infecting microalgae has become an important subject of ecological research, and so far, 63 viruses that infect eukaryotic microalgae have been identified and cultured.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Markus Schulze, Julia Niemann, Rene H. Wijffels, Jens Matuszczyk, Dirk E. Martens
Summary: This study demonstrates a rapid and efficient process intensification strategy by implementing N-1 perfusion and high inoculum cell densities to improve an N-stage fed-batch process, combined with productivity enhancers. By rapidly intensifying the conventional process in small-scale bioreactors, the space-time yield was successfully doubled while maintaining product quality.
BIOTECHNOLOGY PROGRESS
(2022)
Review
Biotechnology & Applied Microbiology
Marta Sa, Narcis Ferrer-Ledo, Fengzheng Gao, Carlo G. Bertinetto, Jeroen Jansen, Joao G. Crespo, Rene H. Wijffels, Maria Barbosa, Claudia F. Galinha
Summary: Microalgae industrial production is seen as an alternative solution for producing nutraceuticals, cosmetics, biofertilizers, and biopolymers. While technological advances have increased the competitiveness of the industry, online monitoring and real-time process control still need further development, with fluorescence spectroscopy being the most promising method for monitoring.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Chunzhe Lu, Nina Leitner, Rene H. Wijffels, Vitor A. P. Martins dos Santos, Ruud A. Weusthuis
Summary: This study developed a biocatalyst for the production of mcl-diols from n-alkanes under mild conditions. The engineered strain P. putida KT2440, with overexpression of Est12, can efficiently hydrolyze esterified diols, enabling the production of mcl-diols.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Markus Schulze, Yadhu Kumar, Merle Rattay, Julia Niemann, Rene H. Wijffels, Dirk E. Martens
Summary: Process intensification is gaining popularity in the mammalian biomanufacturing industry as a result of the increasing demand for biotherapeutics. This study established a CHO cell process and investigated the impact of butyric acid supplementation on the production process. The findings showed that butyric acid supplementation led to higher cellular productivities and had minimal effects on product quality attributes. Additionally, detailed transcriptomic analysis revealed potential mechanisms for gene engineering optimization.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Biochemical Research Methods
Marcel Janssen, Rene H. Wijffels, Maria J. Barbosa
Summary: Microalgae have high protein expression levels and can be produced in contained cultivation systems with low water requirements. Although the current production scale is small, techno economic analysis has shown good potential for scale-up and cost reduction. Microalgal protein has a suitable amino acid composition for food and feed requirements, and high quality can be guaranteed through novel biomass pre-treatment steps.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Robin Barten, Michelle Kleisman, Giulia D'Ermo, Harm Nijveen, Rene H. Wijffels, Maria J. Barbosa
Summary: High temperatures negatively affect cell physiology and reduce growth and productivity in photobioreactors. Supra-optimal temperature causes cell cycle arrest and changes in metabolic activity. It also affects the composition of fatty acids and pigments in microalgae. Gene expression related to photosynthesis, carbon fixation, and metabolism is downregulated, while genes related to proteasome, autophagy, and endocytosis are upregulated.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Christian Sudfeld, Aamna Kiyani, Hortense Buckens, Michal Hubacek, Rene H. Wijffels, Maria J. Barbosa, Sarah D'Adamo
Summary: Medium chain fatty acids (MCFAs) are compounds of commercial interest with applications in various industries. This study successfully transferred an enzyme from Cuphea palustris to Nannochloropsis oceanica microalgae to accumulate MCFAs.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Jesus Ruiz, Rene H. Wijffels, Manuel Dominguez, Maria J. Barbosa
Summary: The debate over the economical choice between heterotrophic and autotrophic cultivation of microalgae continues. A study analyzed the costs and limitations of both methods, finding that autotrophic cultivation shows potential to compete with heterotrophic production if the production costs can be further reduced.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Agricultural Engineering
Iulian Zoltan Boboescu, Antoinette Kazbar, Lars Stegemuller, Piet Lazeroms, Thanasis Triantafyllou, Fengzheng Gao, Calvin Lo, Maria J. Barbosa, Michel H. M. Eppink, Rene H. Wijffels
Summary: Cellular agriculture offers a more sustainable option for food production, and Tisochrysis lutea microalgae is a valuable source of nutrients. Mild targeted acoustic treatment has been found to disrupt cells and reduce energy requirements.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Tiago Castanheira Silva, Michel Eppink, Marcel Ottens
Summary: Biopharmaceuticals, especially monoclonal antibodies (mAb), play a critical role in the treatment of diseases such as cancer and autoimmune diseases. However, the purification step using chromatography is costly and time-consuming. This paper presents a microfluidic chip approach that offers a more cost-effective alternative for chromatographic process development. The microfluidic chip shows comparable performance to other miniaturized techniques, with significantly reduced liquid and resin volume.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mariana N. Sao Pedro, Mafalda S. Santos, Michel H. M. Eppink, Marcel Ottens
Summary: This research presents the development of a real-time process analytical technology tool for monitoring the formation of antibody aggregates. By using miniaturized sensors and a zigzag microchannel structure, fast and efficient mixing is achieved, providing important real-time feedback and process control for fluorescence-based protein aggregate detection.
BIOTECHNOLOGY JOURNAL
(2023)
Review
Biotechnology & Applied Microbiology
Maria J. Barbosa, Marcel Janssen, Christian Sudfeld, Sarah D'Adamo, Rene H. Wijffels
Summary: The urge for food security and sustainability has driven the development of microalgal biotechnology. Microalgae, as microorganisms, have the potential to be a valuable resource for food, feed, energy, and chemicals. With faster growth and higher productivity than plant crops, microalgae have the advantage of not requiring agricultural land and having efficient fertilizer uptake. They are also seen as a replacement for soy protein, fish oil, and palm oil, and can be utilized in industrial biotechnology for various purposes.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Edgar Suarez Garcia, Carlota F. Miranda, M. Teresa Cesario, Rene H. Wijffels, Corjan van den Berg, Michel H. M. Eppink
Summary: A novel extraction and fractionation concept using Ionic Liquids (ILs) was proposed to extract and recover proteins and carbohydrates from macroalgae. The process involved IL-assisted mechanical shear for initial extraction and two-phase partitioning or ultrafiltration for fractionation. The selective extraction of proteins and the preservation of their native structure were demonstrated. The potential application of this approach in the biorefinery of macroalgae was highlighted.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Engineering, Environmental
Lukas M. Trebuch, Jasper Sohier, Sido Altenburg, Ben O. Oyserman, Mario Pronk, Marcel Janssen, Louise E. M. Vet, Rene H. Wijffels, Tania V. Fernandes
Summary: Photogranules are a new wastewater treatment technology that uses solar energy for low-energy water treatment and shows potential for nutrient recovery. In this study, polyphosphate accumulating organisms (PAOs) were introduced to photogranules, resulting in increased phosphorus removal. The addition of a 12-hour anaerobic phase during nighttime did not negatively affect the PAOs and phototrophs but decreased nitrogen removal and biomass productivity.
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)