Article
Chemistry, Analytical
Luxia Yang, Xiaobo Zhou, Ke Zhang, Jinxia Liu, Lingfeng Zhao, Aiting Cai, Xinyuan Zhao, Li Wu, Yuling Qin
Summary: In this study, an electrochemiluminescent (ECL)/electrochemical (EC) ratiometric biosensor was developed for ultrasensitive determination of circulating tumor DNA (ctDNA) using an exonuclease III (Exo III)-assisted target recycling strategy. The ratiometric biosensing was achieved by integrating the electrochemical activity and ECL quenching property of ferrocene (Fc). Additionally, a novel aggregation-induced electrochemiluminescence (AIECL) molecule, named 4,4'-(benzo[c][1,2,5]thiadiazole-4,7-diyl)bis(N, N-diphenylaniline) (TPA-BT), was designed and synthesized to enhance the AIECL emission, enabling the construction of the ratiometric biosensor. The optimized biosensor exhibited a wide detection range (100 aM-100 pM) and a low detection limit (11.2 aM) for ctDNA analysis (KRAS gene), with potential for multiplexed detection of other ctDNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Li Li, Zheng Zhou, Xinmin Li, Dandan Li, Min Zhao, Changjin Liu, Haiping Wu, Wei Yang, Shijia Ding, Bo Shen
Summary: A novel 3D walking nanomachine-based biosensor utilizing strand displacement for ultrasensitive detection of ATP was successfully developed, demonstrating a low limit of detection and practical application in human serum samples. This biosensing strategy holds great potential for biochemical studies and clinical diagnosis.
Article
Chemistry, Analytical
Ruo Yuan, Ya-Qin Chai, Jia-Hang Li, Jia-Li Liu, Xiao-Long Zhang, Xiao-Chun Zhu
Summary: A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed using 2D Co3O4 nanosheets as a coreaction accelerator for the detection of miRNA-21. The Co3O4 nanosheets promoted the decomposition of H2O2 to generate more O2.-, which enhanced the ECL signals by reacting with luminol. Additionally, the biosensor achieved ultrasensitive detection of miRNA-21 through the self-assembly of highly ordered rolling DNA nanomachines.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yu-Hong Cheng, Si-Jia Liu, Jian-Hui Jiang
Summary: A label-free, isothermal, non-enzymatic, and ultrasensitive electrochemical biosensor based on autonomous proximity dependent surface hybridization chain reaction (HCR) was reported for the detection of target survivin mRNA, showing high sensitivity, strong specificity, simplicity, and signal amplification capability.
Article
Chemistry, Analytical
Di Zhang, Xin Yu, Lina Wu, Huali Jin, Min Wei
Summary: A sensitive and selective electrochemical Pb2+ sensor was developed based on DNAzyme and exonuclease III, allowing for a wide detection range and high sensitivity in detecting Pb2+ concentrations through target recycling amplification.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hong Hai, Ciping Chen, Dongli Chen, Peijun Li, Yang Shan, Jianping Li
Summary: An ECL DNA biosensor based on Exolli exonuclease assistance and HCR amplification technology was constructed for high-sensitive detection of transgenic rice BT63 DNA. The sensor utilized ExoIII exonuclease and triple-helix DNA molecular switch, combined with HCR and AuNPs@DNA to enable multiple signal amplification. The detection limit was 3.6 x 10(-17) mol/L with good linear relationship in the concentration range 1.0 x 10(-16) to 1.0 x 10(-8) mol/L of the target DNA.
Article
Chemistry, Analytical
Zhujun Ai, Xinmin Li, Rui Chen, Daobin Han, Huijie Bai, Shaoying Yan, Pengcheng Gu, Haiping Wu, Shijia Ding, Junman Chen, Hua Tang
Summary: Researchers have integrated localized catalytic hairpin assembly (CHA) with nanoscale AuNPs scaffolds to develop a fast three-dimensional DNA walker, which showed significantly higher walking speed compared to other methods. By further integrating it with magnetic probes and g-C3N4 nanosheets, an ultrasensitive and enzyme-free homogeneous electrochemiluminescence (Ho-ECL) aptasensor was constructed for sonic hedgehog (SHh) detection, achieving a low detection limit of 13.5 fM. This work has broadened ideas for the construction of fast DNA walkers and facilitated the development of clinical diagnosis methods.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Environmental
Baozhong Zhang, Panpan Tian, Huina Zhu, Lingling Xie, Pengbo Dai, Baoshan He
Summary: A simple and sensitive electrochemical aptasensor was developed for detecting PCB77 in the environment, with exceptional detection performances including a very low detection limit and a wide linear range. The aptasensor demonstrated high selectivity, stability, and reproducibility, showing great potential for real-world applications.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Applied
Jinri Chen, Nan Sun, Haohan Chen, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: A high-sensitive fluorescent biosensor for Neu5Ac has been developed based on FRET between CdSe/ZnS quantum dots (QDs) and BHQ(2), as well as exonuclease III (Exo III)-assisted recycling amplification strategy. The biosensor achieved an ultralow detection limit of 24 fM and good linear response ranges within 0.2-12.5 pM and 12.5-1000 pM, showing great potential for detection of sialic acids modified glycans/lipids in medical diagnosis and food testing.
Article
Chemistry, Analytical
Shengqiang Li, Zhengxiang Fu, Chao Wang, Xipeng Shang, Yan Zhao, Cuiying Liu, Ming Pei
Summary: The electrochemical biosensor developed for DNA determination utilizes T7 exonuclease-assisted regulatory strand displacement dual recycling signal amplification strategy, showing high sensitivity and selectivity. It has been successfully applied for target DNA detection in serum, demonstrating potential for molecular diagnostics.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Xiao Zhao, Guihua He, Wenfang Deng, Yueming Tan, Qingji Xie
Summary: This study developed a self-powered biosensing platform for highly sensitive miRNA detection by regulating the enzymatic loading capacity on the bioanode using concatenated catalytic hairpin assembly/hybridization chain reaction. The platform showed a wide dynamic range and ultralow detection limit for miRNA-141 detection.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yu-Zhuo Guo, Jia-Li Liu, Yi-Fei Chen, Ya-Qin Chai, Zhao-Hui Li, Ruo Yuan
Summary: By utilizing boron and nitrogen-codoped carbon dots as highly efficient ECL emitters, a novel ternary ECL system was prepared for ultrasensitive detection of HBV-DNA. The BN-CDs showed superior ECL efficiency compared to both nondoped and single-doped CDs. Combining the Exo III-induced DNA amplification strategy, an ECL biosensor was developed for the ultrasensitive detection of HBV-DNA at extremely low concentrations.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Jie Liu, Jie Chen, Yue Zhang, Linlei Liu, Yuzhuo Guo, Ruo Yuan, Yaqin Chai
Summary: In this study, selenium and nitrogen co-doped carbon dots (SeN-CDs) were synthesized as a new emitter with highly efficient electrochemiluminescence (ECL) and excellent biocompatibility for sensitive detection of microRNA-221 (miRNA-221). The SeN-CDs exhibited superior ECL performance compared to N-doped CDs, with selenium playing a key role in enhancing the ECL efficiency. The biosensor constructed using the SeN-CDs and a T7 Exo-assisted double cycle amplification strategy achieved ultrasensitive detection of miRNA-221, with a low detection limit of 2.3 aM. This work provides a novel method to enhance the ECL performance of carbon dots for the development of sensitive biosensing platforms for biomarker detection and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Si Chen, Rong Fang, Yi Li, Fei Deng, Xing Liu, Danting Yang
Summary: A novel approach called the CRISPR/Cas12a enzyme-linked aptamer-sorbent assay (CLASA) was developed for ultrasensitive detection of Ochratoxin A (OTA). This method not only overcomes the limitations of traditional methods, but also has the flexibility to detect other small molecular targets. It holds great promise for applications in various fields requiring sensitive and specific detection of diverse analytes.
MICROCHEMICAL JOURNAL
(2024)
Article
Optics
Jidong Liu, Qiaoyan Hao, Haibo Gan, Peng Li, Benxuan Li, Yudi Tu, Jingting Zhu, Dianyu Qi, Yang Chai, Wenjing Zhang, Fei Liu
Summary: This study presents the synthesis of tellurium nanoflakes with broadband photoresponse to construct a type-I InSe/Te van der Waals heterostructure photodetector. The detector exhibits ultralow dark current, ultrahigh current rectification ratio, considerable photovoltaic effect, and fast response time. Band alignment engineering of the heterostructure enables the development of high-performance self-powered photodetectors.
LASER & PHOTONICS REVIEWS
(2022)
Article
Chemistry, Analytical
Wen Shou, Yueliang Wang, Yuanyuan Yao, Lifen Chen, Binyong Lin, Zhenyu Lin, Longhua Guo
Summary: Monitoring temperature change is crucial for ensuring the safety and efficacy of temperature-sensitive products. Traditional digital thermometers have limitations in cost, recording capabilities, and vulnerability to tampering. Therefore, the development of multicolor plasmonic hydrogels as a full-history time-temperature indicator (FHTTI) provides a valuable solution for accurately and comprehensively indicating the time and temperature history of products.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Qin Luo, Mengjian Tian, Fang Luo, Min Zhao, Cuiying Lin, Bin Qiu, Jian Wang, Zhenyu Lin
Summary: This study established an innovative multicolor sensor for trypsin detection, based on the regulation of the peroxidase activity of BSA-Au NCs and efficient etching of Au NBPs. The designed sensor has a linear relationship with the logarithm of trypsin concentration, with a detection limit of 0.045 μg/mL. It has been successfully applied to analyze trypsin concentration in human serum samples from healthy individuals and pancreatitis patients.
ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Tong Li, Ruiguo Wang, Ruijie Yin, Hongyan Xu, Xiaoxu Han, Qiuling Du, Jie Cheng, Zhenyu Lin, Peilong Wang
Summary: A novel Zr(IV)-based metal-organic framework (MOF), named BUT-16, was synthesized and utilized for the extraction and enrichment of bisphenol compounds (BPs) in milk samples. The method showed a detection limit as low as 0.05 ng/mL and was successfully applied to the detection of BPs in real milk samples, yielding results consistent with previous methods.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Liang Wang, Ying Cheng, Cuiqin Wu, Fang Luo, Zhenyu Lin, Ravi Naidu
Summary: The study used SPME to sample soil gas and GC/MS to detect petroleum hydrocarbon volatile organic compounds (pH-VOCs) in underground pipeline leaks. By optimizing the extraction method and sample conditioning, the analytical efficiency and accuracy were improved, resulting in reliable research data with minimized environmental impacts.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Liangzhen Xu, Shuang Hou, Xiaocui Huang, Mengyuan Wang, Cheng Li, Nuo Dong, Zhenyu Lin
Summary: A homogeneous electrochemiluminescence (ECL) biosensor based on catalyze hairpin assembly (CHA) and hybridization chain reaction (HCR) and magnetic assisted enrichment has been developed for miR-155 detection in dry eye diseases caused by Sjogren's syndrome. The biosensor offers a simple and sensitive detection method and has been successfully applied in clinical samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weiquan Wang, Yunpeng Xu, Jie Hou, Zhixin Li, Fang Luo, De He, Zhenyu Lin
Summary: A HAase-responsive hydrogel system was designed to semi-quantitatively determine HAase by utilizing the relationship between HAase and spilled H2O2. Additionally, Au NRs were etched by H2O2, and the color change of the solution was used as a semi-quantitative method for HAase determination. The proposed method has been successfully applied to monitor HAase in serum of pancreatic cancer patients.
Article
Chemistry, Analytical
Peiqing Luo, Xiaocui Huang, Fang Luo, Zhonghui Chen, Yu Chen, Cuiying Lin, Jian Wang, Bin Qiu, Zhenyu Lin
Summary: In this study, a sensitive ECL biosensor for hepatitis B virus DNA detection was developed by utilizing the high amplification efficiency of hybridization chain reaction (HCR), high specificity of the CRISPR/Cas12a system, and convenience of the homogeneous electrochemiluminescence (ECL) assay. The sensor exhibits high reproducibility, accuracy, and sensitivity, making it a promising tool for clinical testing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qin Luo, Yu Chen, Lifen Chen, Zhonghui Chen, Fang Luo, Jian Wang, Cuiying Lin, Bin Qiu, Longhua Guo, Zhenyu Lin
Summary: A multicolor sensor based on the inhibition of peroxidase activity and color change caused by etching of gold nanobipyramids was constructed for Hg2+ detection. The sensor can achieve semi-quantitative detection using the multicolor changes of the gold nanobipyramids and quantitative sensing by measuring the UV-vis peak shift. The linear response range is 1.0 nM-10 μM, and the detection limit is 0.28 nM.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yunpeng Xu, Jiahui Chen, Xiaolu Sui, Yanzi Zhang, Aisha Zhang, Zhenyu Lin, Xinguang Liu, Jihong Chen
Summary: The study developed an ultra-sensitive biosensor for microRNA-21 (miRNA-21) by combining the high selectivity and trans-cleavage activity of CRISPR/Cas13a, the high amplification efficiency of HCR, and the high sensitivity of ECL detection. The biosensor recognized target RNA using CRISPR/Cas13a and performed trans-cleavage activity. A signal amplification was achieved by generating long dsDNAs through HCR, and ECL intensity was measured using Ru(phen)32+ as an indicator. The system showed good linear correlation and a limit of detection (LOD) of 0.53 fM for miRNA-21, and was successfully applied to detect miRNA-21 in the urine of acute kidney injury (AKI) patients.
Article
Chemistry, Analytical
Yifan Jiang, Huilan Zhang, Meizhu Xu, Fang Luo, Cuiying Lin, Bin Qiu, Zhenyu Lin, Zhou Jiang, Jian Wang
Summary: A novel photoelectrochemical (PEC) sensor was constructed for highly sensitive detection of reduced glutathione (GSH) using the catalytic properties of phosphotungstic acid (PTA). PTA acts as an electron acceptor, increasing the photogenerated current of Cu2O by inhibiting electron-hole complexation. It also facilitates the regeneration of GSH by reducing oxidized glutathione (GSSG) through proton transfer. Additionally, PTA pre-oxidizes interfering substances, improving the selectivity of the method. The PEC sensor showed a linear response to GSH in the range of 0.050-100 nmol L-1, with a low detection limit of 0.017 nmol L-1 (S/N = 3) for GSH content detection in cell lysate samples.
Article
Chemistry, Analytical
Qin Luo, Mengjian Tian, Fang Luo, Min Zhao, Cuiying Lin, Bin Qiu, Jian Wang, Zhenyu Lin
Summary: In this study, a novel multicolor sensor for trypsin detection has been developed based on the regulation of peroxidase activity of BSA-Au NCs. By hydrolyzing BSA ligands on the surface of BSA-Au NCs, trypsin exposes more catalytic active sites of BSA-Au NCs, enhancing their peroxidase activity, resulting in the generation of more TMB+. Under acidic conditions, TMB+ effectively etches Au NBPs, changing their aspect ratio and causing a blue shift in the UV-vis spectra.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weixin Li, Xinyang Wang, Jiaji Huang, Min Zhao, Jiao Yang, Fang Luo, Bin Qiu, Jian Wang, Zhenyu Lin
Summary: In this work, three-dimensional ZnO nanostructures were used as the support for gold nanoparticles and graphite carbon nitride to form a Z-scheme heterostructure photoelectrode. The photoelectrode exhibited fast carrier transport and abundant reaction sites, and the gold nanoparticles acted as mediators to enhance electron transfer. The developed photoelectrochemical biosensor based on the Z-scheme heterostructure showed good sensitivity and selectivity for the detection of reduced glutathione.
SENSORS & DIAGNOSTICS
(2023)
Article
Chemistry, Analytical
Cheng Chen, Jing Li, Fang Luo, Zhenyu Lin, Jian Wang, Tao Zhang, Aiwen Huang, Bin Qiu
Summary: In this study, a Eu MOF was used to encapsulate FeNCDs, resulting in increased dispersibility and enzymatic catalytic efficiency. The FeNCDs@Eu MOF-based sensor exhibited a nearly 6-fold increase in response to tetracycline, with a low detection limit of 11.63 nM.
Article
Chemistry, Analytical
Peiqing Luo, Xiaocui Huang, Fang Luo, Zhonghui Chen, Yu Chen, Cuiying Lin, Jian Wang, Bin Qiu, Zhenyu Lin
Summary: In this study, a sensitive ECL biosensor for hepatitis B virus DNA was developed by combining HCR amplification, CRISPR/Cas12a system, and regulation of negative charge on reporting probes. The sensor exhibited low background, high reproducibility, and high sensitivity, and showed high accuracy in detecting clinical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Weixin Li, Xinyang Wang, Lifen Chen, Fang Luo, Longhua Guo, Cuiying Lin, Jian Wang, Bin Qiu, Zhenyu Lin
Summary: This study proposes a homogeneous photoelectrochemical sensor based on three-dimensional ZnO/Au/two-dimensional MoS2 for ultrasensitive detection of tetracycline (TET). MoS2 is uniformly embedded on the 3D ZnO/Au surface using ordered self-assembly, replacing the conventional chemical modification method. Under optimal conditions, this method has successfully detected TET in milk, honey, pig kidney, and pork samples with reliable results. We believe that this study presents a method for the preparation of sensing carriers and target detection with great potential for application.