Article
Chemistry, Analytical
Yi Shen, Haoyu Yuan, Zixuan Guo, Xiu-Qing Li, Zhiqing Yang, Chengli Zong
Summary: Exonuclease III (Exo III) is commonly used as a dsDNA-specific exonuclease that doesn't degrade ssDNA. However, this study demonstrates that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear ssDNA. Additionally, the dsDNA specificity of Exo III is crucial in DNA target recycling amplification (TRA) assays. The findings expand the substrate scope of Exo III and have implications for its experimental applications.
Article
Biochemical Research Methods
Huali Jin, Ruike Liu, Tian Bai, Min Wei, Baoshan He, Zhiguang Suo
Summary: A low-noise ratiometric fluorescence biosensor based on Pb2+-dependent DNAzyme and exonuclease III (Exo III)-assisted cascade signal amplification was developed for the detection of lead ions. The biosensor exhibited a lower limit of detection and a wider detection range compared to other methods, and showed satisfactory results in the detection of lead ions in tap water, tea, and rice flour samples. This ratiometric fluorescence biosensor has great potential in various target monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Spectroscopy
Boxu Wang, Zheng Liu, Zhihong Li, Ningyi Xu, Xuejiao Zhang, Ruifang Su, Junyang Wang, Rui Jin, Chunyan Sun
Summary: In this study, a fluorescent probe was constructed for facile and sensitive detection of Hg2+ based on the unique fluorescent property of 2-aminopurine (2-AP). The probe exhibited an excellent linear relationship with Hg2+ concentration and had a low detection limit. Satisfactory detection results were obtained in water and fish samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Di Zhang, Xin Yu, Lina Wu, Huali Jin, Min Wei
Summary: A sensitive and selective electrochemical Pb2+ sensor was developed based on DNAzyme and exonuclease III, allowing for a wide detection range and high sensitivity in detecting Pb2+ concentrations through target recycling amplification.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Luyang Zhao, Zhiguang Suo, Baoshan He, Yawei Huang, Yong Liu, Min Wei, Huali Jin
Summary: A novel nitrogen-doped carbon supported palladium material (C-N-Pd) was synthesized and used in constructing a fluorescent aptasensor for AFB1 detection. The aptasensor exhibited high sensitivity and good selectivity towards AFB1, and successfully detected the toxin in contaminated food samples. The aptasensor showed good agreement with the HPLC method, indicating its potential as a reliable detection tool for AFB1.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ghasem Anbiaee, Zahra Khoshbin, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Developing cost-effective smart sensing tools with ultrahigh sensitivity for food safety insurance is highly important. Aptamer-based biosensors powered by recycling signal amplification strategies, such as target recycling supported by enzymes, have shown great potential in achieving signal amplification. Exonucleases, as superior biocatalysts, simplify the amplification process and exhibit outstanding selectivity and sensitivity, making them promising sensing tools for label-free, ease-of-use, cost-effective, and real-time diagnosis of diverse targets. This article summarizes the achievements and perspectives in aptasensor design for qualitative monitoring of diverse targets using exonucleases for signal amplification.
Article
Food Science & Technology
Chengyuan Wu, Xinjie Wang, Ling Guo, Xingxu Huang, Lina Wu, He Huang
Summary: A neoteric electrochemical biosensor based on an exonuclease III (Exo III)-assisted recycling reaction triggering the trans-cleavage ability of CRISPR-Cas12a was developed for the selective and ultrasensitive detection of ochratoxin A (OTA). The method showed good selectivity and sensitivity for OTA determination with a detection limit of 0.74 fg/mL. Furthermore, the established method could sensitively identify OTA in spiked rice and peanut samples, demonstrating its application potential for practical detection.
Article
Chemistry, Analytical
Xuechen Zhang, Yao Gao, Jiawen Li, Xue Fan, Renhuan Song, Wenbo Song
Summary: To discriminate the signals of multi-targets in photoelectrochemical (PEC) detection, a non-instrument-assisted strategy for signal discrimination is proposed. This strategy utilizes exonuclease III-mediated multiple PEC signals resolution and a self-cleaning recyclable multiplexed PEC sensor. By cleaving the DNA strand attached to the magnetic bead, the signal labels can be separated, allowing for the generation of distinguishable photocurrent signals of different targets on a single photoelectric interface. The feasibility of this multiplexed sensor is verified by detecting two coexisting mycotoxins. The proposed sensing strategy eliminates instrumentation constraints and simplifies experimental procedures, providing a new idea for portable multiplexed PEC sensing devices.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chunhong Zhou, Yihang Zhang, Mingjuan Huang, Ke Yang, Jiuying Tian, Jusheng Lu
Summary: By utilizing the specific recognition of anti-thrombin aptamer towards thrombin, ingenious design of hairpin DNA, and exonuclease III-assisted recycling signal amplification, a sensitive and selective TNA/g-C3N4/c-DNA photoelectrochemical aptasensor was developed for determining thrombin. This aptasensor exhibited a satisfactory quantitative range, low detection limit, and successfully applied for human serum analysis.
Article
Chemistry, Analytical
Ruike Liu, Baoshan He, Huali Jin, Zhiguang Suo
Summary: A fluorescent aptasensor was developed for the detection of lead ion. The sensor exhibited high selectivity and sensitivity, making it suitable for the identification of lead ions in real samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Siling Chen, Rongjun Yu, Ying Li, Jiangling Wu, Jingfu Qiu, Xinyi Huang, Jianjiang Xue
Summary: In this study, a new biosensor was developed for the sensitive detection of the CYP2C19*2 gene. The biosensor utilized exonuclease III assisted electric signal amplification and calixarene for the enrichment of signal substances. The method demonstrated good selectivity, high sensitivity, and stability, making it a promising tool for the detection of the CYP2C19*2 gene and other biological molecules.
Article
Chemistry, Analytical
Guangming Li, Hang Gao, Ningning Wang, Zhaohui Li, Huangxian Ju
Summary: This study proposed a simple and sensitive method for ratiometric fluorescent detection of nucleic acids by controlling the pH-dependent adsorption of dye-labeled DNA on polymer dots. The method showed promising application in bioanalysis with excellent performance and good extendability.
ANALYTICA CHIMICA ACTA
(2022)
Article
Cell Biology
Mikhail E. Matlashov, Jorge Vera, Ludmila A. Kasatkina, Kamran Khodakhah, Vladislav V. Verkhusha
Summary: Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are powerful tools in neuroscience due to their spectral characteristics that minimize signal loss and cross-excitation artifacts. However, currently available NIR GECIs lack high brightness, photostability, small size, and fast response kinetics. In this study, we introduce a small FRET-based NIR fluorescent calcium indicator called iGECInano, which demonstrates improved signal-to-noise ratio and response kinetics compared to other NIR GECIs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Qirong Chen, Huahui Gao, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a new target/aptamer binding-induced inhibition of enzyme activity strategy was used to construct an amplified and sensitive electrochemical biosensor for detecting Sonic Hedgehog (SHh). The sensor exhibited high selectivity and could detect SHh down to 3.34 pM. This strategy has the potential to be a convenient approach for detecting different proteins in biological studies and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Panting Sang, Zhigang Hu, Yuliang Cheng, Hang Yu, Fangwei Yang, Yunfei Xie, Weirong Yao, Yahui Guo, He Qian
Summary: An integrated approach combining immunoassay and nucleic acid amplification was developed for the sensitive detection of chloramphenicol. The method, called exonuclease III-assisted nucleic acid amplification fluorescence immunoassay (EANAFIA), showed a detection linear range from 0.01 to 5 ng/L with a low detection limit of 0.001 ng/L. The assay demonstrated no cross reaction with other antibiotics and was successfully applied in detecting chloramphenicol in skim milk with acceptable recoveries. This integrated strategy showcased a hybrid performance of indirect immunoassay with ultra-sensitive sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.