Article
Biology
Chen Xu, Chenyu Liu, Kaichao Chen, Ping Zeng, Edward Wai Chi Chan, Sheng Chen
Summary: The drug otilonium bromide restores the activity of colistin against colistin-resistant Gram-negative bacteria and reduces toxicity, it also exhibits synergistic effect in eradicating multidrug-tolerant persisters of Gram-negative bacteria.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Md Rezaul Karim, Zunita Zakaria, Latiffah Hassan, Nik Mohd Faiz, Nur Indah Ahmad
Summary: The prevalence of colistin resistance and its molecular mechanisms were studied in Enterobacteriaceae isolated from chicken and chicken meats. Among 627 isolates, 8.6% demonstrated phenotypic colistin resistance, and 12.96% of these resistant isolates carried mcr genes. Specifically, mcr-1 and mcr-5 genes were detected in 11.11% and 1.85% of the colistin-resistant isolates, respectively. This study also identified the first report of the mcr-5 gene in Malaysia. Further investigations are required to characterize mcr-5 harboring bacteria and their associated plasmids.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Md. Rezaul Karim, Zunita Zakaria, Latiffah Hassan, Nik Mohd Faiz, Nur Indah Ahmad
Summary: This study found that colistin-resistant Enterobacteriaceae isolates recovered from poultry and poultry meats carry multiple drug-resistance genes, including the mcr gene. These isolates often exhibit multi-drug resistance and pose potential threats to infection treatment choices for humans and animals.
Article
Infectious Diseases
Yasuhide Kawamoto, Norihito Kaku, Norihiko Akamatsu, Kei Sakamoto, Kosuke Kosai, Yoshitomo Morinaga, Norio Ohmagari, Koichi Izumikawa, Yoshihiro Yamamoto, Hiroshige Mikamo, Mitsuo Kaku, Kazunori Oishi, Katsunori Yanagihara
Summary: This study investigated the prevalence of colistin resistance and mcr gene carriage in ESBL and CRE isolates in Japan. The results showed that the frequency of colistin resistance and mcr-positive strains is not high in Japan, but continuous monitoring of mcr genes is necessary.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Pegah Kananizadeh, Tatsuya Tada, Satoshi Oshiro, Tomomi Hishinuma, Mari Tohya, Yuki Uehara, Yumi Kumagai, Isao Nagaoka, Kanako Nishi, Masahito Hashimoto, Shin Watanabe, Teruo Kirikae
Summary: Three isolates of the Enterobacter cloacae complex carrying the mcr-9 gene were found to be susceptible to colistin, but the minimum inhibitory concentrations (MICs) varied under different test conditions. Colistin was able to induce the expression of mcr-9 gene in a dose-dependent manner and increase the survival rates of the isolates against human antimicrobial peptide LL-37. Screening of the isolates using specific agar medium revealed the presence of mcr-9 gene and other colistin-resistant isolates.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Review
Immunology
Masego Mmatli, Nontombi Marylucy Mbelle, John Osei Sekyere
Summary: This research found that mcr genes are widely distributed in Enterobacteriaceae species and pose a threat to public health as they are mainly transmitted to humans through food animals. The dissemination of mcr genes is mediated by mobile genetic elements and there are compensatory adaptation mechanisms. Combination therapy is a promising option for managing and treating colistin-resistant Enterobacteriaceae infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Firdoos Ahmad Gogry, Mohammad Tahir Siddiqui, Insha Sultan, Qazi Mohd Rizwanul Haq
Summary: The global interest in colistin has risen due to the prevalence of multidrug-resistant Gram-negative Enterobacteriaceae, leading to the emergence of colistin-resistant bacteria. However, some Gram-negative species are intrinsically resistant to colistin, and most identified colistin resistance involves modification of lipid A to decrease or remove interaction with colistin. Changes in membrane structure, specifically in the outer membrane lipopolysaccharide (LPS), play a key role in colistin resistance mechanisms. New methods and approaches are being developed to detect and combat colistin resistance, with a focus on developing less toxic and more effective drugs to treat bacterial infections.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Kaouther Ben Romdhane, Lilia Messadi, Jean Marc Rolain
Summary: The emergence of multidrug-resistant bacteria is a major concern, and cockroaches may contribute to the spread of these bacteria between the environment and humans. This study investigated the presence of drug-resistant Enterobacteriaceae in cockroaches collected from various locations in Tunisia. The findings suggest that cockroaches can act as carriers of drug-resistant bacteria, posing a risk for nosocomial infections in hospitals.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Qian Zhao, Yiming Li, Yingxin Tian, Yueyun Shen, Shaolin Wang, Ying Zhang
Summary: In this molecular epidemiological study, the prevalence of MCRPE in Chinese patients from 2009 to 2019 was investigated. The study found a decline in MCRPE prevalence in China after the banning of colistin usage as a feed additive in 2017. However, the permission of colistin usage in clinical therapy may still increase the risk of MCRPE transmission and intractable infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shengnan Xie, Li Li, Baihe Zhan, Xue Shen, Xuming Deng, Wenxi Tan, Tianqi Fang
Summary: The study identified pogostone as a potential inhibitor of the MCR-1 gene, which can restore the bactericidal activity of colistin against MCR-1-positive bacterial strains and effectively treat severe infections caused by MCR-1-positive Enterobacteriaceae.
Article
Microbiology
Akihiro Nakamura, Tatsuya Nakamura, Makoto Niki, Tomokazu Kuchibiro, Isao Nishi, Masaru Komatsu
Summary: This study investigated the colistin MICs and molecular characteristics of ESBL-Ec and CPE strains in Japan, finding no microbiological or clinical resistance to colistin. Despite the global spread of Enterobacteriaceae carrying mcr-9, the mechanism of colistin resistance by mcr-9 remains unclear.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Yolandi Snyman, Andrew C. Whitelaw, Jo M. Barnes, Motlatji R. B. Maloba, Mae Newton-Foot
Summary: The study found colistin resistant Gram-negative bacteria and mcr genes in rivers and storm water in the Western Cape region, including mcr-5.1 and various mcr-3 gene variants. These genes were mainly present in water sources influenced by human activities, potentially leading to gene transfer to pathogenic bacteria.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Microbiology
Christian Xedzro, Toshi Shimamoto, Liansheng Yu, Hui Zuo, Yo Sugawara, Motoyuki Sugai, Tadashi Shimamoto
Summary: This study reports the emergence of Enterobacter cloacae and Raoultella ornithinolytica carrying the mcr-9.1 gene in vegetables from Japan. The presence of colistin-resistant bacteria in vegetables is a significant public health concern. Monitoring and preventing the dissemination of mcr homologs in the vegetable industry is crucial.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Yancheng Yao, Swapnil Doijad, Jane Falgenhauer, Judith Schmiedel, Can Imirzalioglu, Trinad Chakraborty
Summary: Bacterial infections with highly drug-resistant Enterobacter species are difficult to treat and pose a significant threat to public health. These bacteria exhibit resistance to multiple classes of antibiotics and carry a high number of antibiotic resistance genes and heavy metal resistance genes. The emergence of such strains raises concerns about the effectiveness of current treatment options and the ability to control their spread.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Hongwei Zhou, Siheng Wang, Yuchen Wu, Ning Dong, Xiaoyang Ju, Chang Cai, Ruichao Li, Yan Li, Congcong Liu, Jiayue Lu, Edward Wai-Chi Chan, Sheng Chen, Rong Zhang, Zhangqi Shen
Summary: This study investigated the prevalence of mcr-9 and mcr-10 in Enterobacter cloacae complex (ECC) strains in China and found that the coexistence and co-transfer of mcr genes and carbapenemase genes pose an urgent threat to public health.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Multidisciplinary Sciences
John Osei Sekyere, Eric Mensah
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2020)
Article
Biotechnology & Applied Microbiology
N. Mbelle, J. Osei Sekyere, C. Feldman, N. E. Maning, L. Modipane, S. Y. Essack
LETTERS IN APPLIED MICROBIOLOGY
(2020)
Review
Multidisciplinary Sciences
John Osei Sekyere, Nontuthuko E. Maningi, Petrus B. Fourie
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2020)
Article
Infectious Diseases
Calvina E. L. Estaleva, Tomas F. Zimba, John Osei Sekyere, Usha Govinden, Hafizah Y. Chenia, Gunnar S. Simonsen, Bjorg Haldorsen, Sabiha Y. Essack, Arnfinn Sundsfjord
Summary: This study investigated cephalosporin-resistant clinical strains of Escherichia coli in Mozambique, revealing a high prevalence of MDR pAmpC- and/or ESBL-producing isolates with FOX/MOX and CTX-Ms as the major beta-lactamase types. Genetic analysis showed diversity among strains with some clusters indicating within-hospital spread. These findings emphasize the urgent need for accurate diagnostics and infection control measures.
BMC INFECTIOUS DISEASES
(2021)
Article
Microbiology
John Osei Sekyere, Melese Abate Reta
Summary: Citrobacter spp., Enterobacter hormaechei subsp., Klebsiella variicola and Proteae tribe members are rare Enterobacterales increasingly implicated in nosocomial infections. These species, containing multiple antibiotic resistance genes, are widely distributed globally and can easily transmit these genes to more pathogenic species. Surveillance studies are necessary to identify and contain these antibiotic-resistant species.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Microbiology
John Osei Sekyere, Melese Abate Reta
Review
Microbiology
Masego Mmatli, Nontombi Marylucy Mbelle, Nontuthuko E. Maningi, John Osei Sekyere
Article
Genetics & Heredity
Katlego Kopotsa, Nontombi M. Mbelle, John Osei Sekyere
MICROBIAL GENOMICS
(2020)
Review
Multidisciplinary Sciences
John Osei Sekyere, Melese Abate Reta, Petrus Bernard Fourie
Summary: The study highlights the increasing resistance to carbapenem and polymyxin antibiotics among pregnant women and infants. Different regions show variations in the types and distribution of resistant bacteria. Common risk factors associated with carbapenem resistance include antibiotic therapy and prolonged hospitalization.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Tumisho Mmatumelo Seipei Leshaba, Nontombi Marylucy Mbelle, John Osei Sekyere
Summary: The emergence of polymyxin resistance poses a threat to public and animal health due to transferable mcr genes. Current and emerging polymyxin resistance diagnostics include phenotypic and molecular methods, with Rapid ResaPolymyxin NP test and Fastinov (R) recommended for initial screening due to their fast turnaround time, high sensitivity, and specificity.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Review
Multidisciplinary Sciences
Suzan Mohammed Ragheb, Usha Govinden, John Osei Sekyere
Summary: Carbapenemases, associated with MGEs, are found in bacterial isolates in Africa. Challenges remain for epidemiological analysis of carbapenemases due to technical and budgetary limitations.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Review
Microbiology
Winnie Thabisa Ramaloko, John Osei Sekyere
Summary: This study analyzed Gram-negative bacteria with co-resistance to carbapenems and polymyxins from a One Health perspective. The results showed that these bacteria were widely reported in Asian countries and isolated from clinical, animal, and environmental sources. Colistin resistance was mainly mediated by mgrB inactivation and mcr-1 gene, while OXA-48/181 was the most common carbapenemase. The study also identified bacteria co-resistant to other antibiotics and detected virulence genes and capsule typing.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Masego Mmatli, Tumisho Mmatumelo Seipei Leshaba, Lebogang B. Skosana, Nontombi Marylucy Mbelle, John Osei Sekyere
Summary: This study evaluated the epidemiology of carbapenemases and mcr in Pretoria. The results showed a high prevalence of carbapenemase genes and a low prevalence of mcr genes in Klebsiella pneumoniae, highlighting the need for careful use of antibiotics to prevent further escalation and outbreak of multi-drug resistant infections.
MICROBIAL DRUG RESISTANCE
(2022)
Review
Immunology
Masego Mmatli, Nontombi Marylucy Mbelle, John Osei Sekyere
Summary: This research found that mcr genes are widely distributed in Enterobacteriaceae species and pose a threat to public health as they are mainly transmitted to humans through food animals. The dissemination of mcr genes is mediated by mobile genetic elements and there are compensatory adaptation mechanisms. Combination therapy is a promising option for managing and treating colistin-resistant Enterobacteriaceae infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
John Osei Sekyere, Ayodeji B. Oyenihi, Jason Trama, Martin E. Adelson
Summary: Since 2005, the pathogen BVAB has been identified in the female vaginal tract using PCR, but its actual species was unknown. Without the full genome of these pathogens, we cannot fully understand their pathogenicity, treatment, resistance, and diagnostics. Through sequence homology and phylogenetics analysis, we have identified the actual species of BVAB-1, -2, and -3 and found them to be anaerobic and uncultured species that can only be identified through metagenomics.
MICROBIOLOGY SPECTRUM
(2023)