Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates

Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells

(2018) Xuan Yao et al.   DEVELOPMENTAL CELL

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

(2018) Bin Gu et al.   NATURE BIOTECHNOLOGY

Efficient mouse genome engineering by CRISPR-EZ technology

(2018) Andrew J Modzelewski et al.   Nature Protocols

Switchable genome editing via genetic code expansion

(2018) Toru Suzuki et al.   Scientific Reports

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

(2018) Natasa Savic et al.   eLife

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

(2018) Hyo Min Park et al.   Nature Communications

Integrating Combinatorial Lipid Nanoparticle and Chemically Modified Protein for Intracellular Delivery and Genome Editing

(2018) Jin Chang et al.   ACCOUNTS OF CHEMICAL RESEARCH

Genome editing via delivery of Cas9 ribonucleoprotein

(2017) Mark A. DeWitt et al.   METHODS

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

(2017) Daniel E Ryan et al.   NUCLEIC ACIDS RESEARCH

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

(2017) Jared Carlson-Stevermer et al.   Nature Communications

Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

(2017) Kunwoo Lee et al.   eLife

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

(2017) Megan Basila et al.   PLoS One

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing

(2016) Melissa L. Kelley et al.   JOURNAL OF BIOTECHNOLOGY

PEGylation of Biopharmaceuticals: A Review of Chemistry and Nonclinical Safety Information of Approved Drugs

(2016) Peter L. Turecek et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles

(2016) Ming Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody-drug conjugates: recent advances in conjugation and linker chemistries

(2016) Kyoji Tsuchikama et al.   Protein & Cell

Synthesis and Incorporation of Unnatural Amino Acids To Probe and Optimize Protein Bioconjugations

(2015) Johnathan C. Maza et al.   BIOCONJUGATE CHEMISTRY

Advances in Chemical Protein Modification

(2015) Omar Boutureira et al.   CHEMICAL REVIEWS

Homogeneously modified immunoglobulin domains for therapeutic application

(2015) Tao Liu et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

Optical Control of CRISPR/Cas9 Gene Editing

(2015) James Hemphill et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

Unnatural amino acids in novel antibody conjugates

(2014) Trevor J Hallam et al.   Future Medicinal Chemistry

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Selective chemical protein modification

(2014) Christopher D. Spicer et al.   Nature Communications

SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS AND THEIR APPLICATIONS TO THERAPEUTIC ANTIBODIES

(2014) Remko van Vught et al.   Computational and Structural Biotechnology Journal

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

(2013) Seung Woo Cho et al.   GENETICS

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

An Evolved Aminoacyl-tRNA Synthetase with Atypical Polysubstrate Specificity,

(2011) Douglas D. Young et al.   BIOCHEMISTRY