Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
出版年份 2017 全文链接
标题
Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
作者
关键词
Electroporation, Lipids, Transfection, Health care, CRISPR, Gene knockout, Messenger RNA, Toxicity
出版物
PLoS One
Volume 12, Issue 11, Pages e0188593
出版商
Public Library of Science (PLoS)
发表日期
2017-11-28
DOI
10.1371/journal.pone.0188593
参考文献
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注意:仅列出部分参考文献,下载原文获取全部文献信息。- High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries
- (2017) Žaklina Strezoska et al. JOURNAL OF BIOTECHNOLOGY
- Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing
- (2016) Melissa L. Kelley et al. JOURNAL OF BIOTECHNOLOGY
- Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening
- (2016) Jenille Tan et al. PLoS One
- Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells
- (2016) M. A. DeWitt et al. Science Translational Medicine
- Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy
- (2015) Jennifer L. Gori et al. HUMAN GENE THERAPY
- Challenges in CRISPR/CAS9 Delivery: Potential Roles of Nonviral Vectors
- (2015) Ling Li et al. HUMAN GENE THERAPY
- Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity
- (2015) Emily M. Anderson et al. JOURNAL OF BIOTECHNOLOGY
- Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
- (2015) Xiquan Liang et al. JOURNAL OF BIOTECHNOLOGY
- In vivo genome editing using Staphylococcus aureus Cas9
- (2015) F. Ann Ran et al. NATURE
- Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
- (2015) Ayal Hendel et al. NATURE BIOTECHNOLOGY
- Generation of knock-in primary human T cells using Cas9 ribonucleoproteins
- (2015) Kathrin Schumann et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells
- (2015) Wulan Deng et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
- (2014) Hiroshi Nishimasu et al. CELL
- Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
- (2014) S. Kim et al. GENOME RESEARCH
- Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
- (2013) Seung Woo Cho et al. NATURE BIOTECHNOLOGY
- CRISPR interference (CRISPRi) for sequence-specific control of gene expression
- (2013) Matthew H Larson et al. Nature Protocols
- crRNA and tracrRNA guide Cas9-mediated DNA interference inStreptococcus thermophilus
- (2013) Tautvydas Karvelis et al. RNA Biology
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- RNA-programmed genome editing in human cells
- (2013) Martin Jinek et al. eLife
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
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