Article
Biophysics
Yi-Chen Du, Si-Yuan Wang, Ya-Xin Wang, Jia-Yi Ma, Dong-Xia Wang, An-Na Tang, De-Ming Kong
Summary: A highly sensitive biosensing strategy utilizing TdT and CRISPR-Cas12a for signal amplification was reported, showing great potential in the design of various biosensors and achieving ultrasensitive detection of UDG and T4 PNK. The biosensors developed based on this strategy display high sensitivity, signal amplification efficiency, and low background noise, making them promising for clinical diagnosis and biomedical research.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xia Cheng, Huahua Song, Dandan Ren, Mingcong Gao, Xinyi Xia, Ping Yu, Xiaolan Bian
Summary: This study demonstrates a sensitive UDG fluorescent assay based on rolling circle transcription (RCT)/CRISPR/Cas12a-assisted bicyclic cascade amplification strategy. The method allows for sensitive and accurate detection of UDG, which is crucial for early clinical diagnosis. Target UDG is amplified via RCT and CRISPR/Cas12a, and the whole reaction is completed without complex procedures.
Article
Chemistry, Multidisciplinary
Feifei Luo, Xing Geng, Zhi Li, Ge Dai, Zhaohui Chu, Pingang He, Fan Zhang, Qingjiang Wang
Summary: A post-assembly CRISPR-Cas12a method based on target-induced transcription amplification and real-time crRNA generation has been proposed for bacterial 16S rDNA biosensing. This label-free method eliminates the requirement of a protospacer adjacent motif (PAM) on the target sequences, and shows potential as an effective and simple approach for nucleic acid detection and infectious disease diagnosis.
Article
Nanoscience & Nanotechnology
Shaoqiong Feng, Hanjun Chen, Ziao Hu, Tingting Wu, Zhihong Liu
Summary: A distance-based biosensor using hydrogel, CRISPR/Cas12a and target-triggered strand displacement amplification (SDA) reaction is proposed for the visual, quantitative and sensitive measurement of microRNA. The biosensor can detect the concentration of target microRNA visually without the assistance of instruments, and it shows high sensitivity and specificity, making it suitable for point-of-care applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Dandan Ren, Qiutong Chen, Xinyi Xia, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu, Yao Cen
Summary: In this study, a new tandem system was developed for sensitive detection of cytokine IFN-gamma. This system combined aptamer, strand displacement amplification (SDA), CRISPR/Cas12a, and cobalt oxyhydroxide nanosheets for signal amplification, resulting in improved detection sensitivity. The study demonstrated the potential of this method for clinical cytokine assay and cytokine storm prevention.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yuanzhao Hu, Yuefeng Qiao, Xiu-Qing Li, Zhenbo Xiang, Yi Wan, Peng Wang, Zhiqing Yang
Summary: Rapid, efficient, specific, and sensitive diagnostic method called DTDP was developed utilizing Cas9n and Cas12a to select appropriate treatments for drug-resistant bacterial infections. The DTDP works by creating a nick in the target dsDNA using Cas9n-sgRNA complex, and DNA polymerase displaces ssDNA leading to cycles of DNA replication. The ssDNA is detected by Cas12a-crRNA complex, generating a fluorescent signal. DTDP exhibits high sensitivity, specificity, and accuracy, and the dual RNA recognition process improves diagnostic specificity.
Article
Chemistry, Analytical
Min Qing, Zhe Sun, Lei Wang, Shi Zhe Du, Jiao Zhou, Qian Tang, Hong Qun Luo, Nian Bing Li
Summary: The study combines electrochemical aptasensors with the CRISPR/Cas12a strategy, utilizing BIDSD, RCA, and CRISPR/Cas12a mechanisms to achieve specific and sensitive detection of thrombin with a calculated limit of detection of 1.26 fM, while simplifying the operation steps by omitting the probe surface-immobilization procedure.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Jiarui Zhang, Rong Zhao, Kejun Dong, Wan Shu, Sitian Wei, Yuwei Yao, Jun Zhang, Shuangge Liu, Wei Zhang, Hongbo Wang
Summary: DNA damage is a major cause of diseases, and UDG plays a crucial role in repairing DNA damage and serves as an early diagnostic marker and prognostic indicator for cancer. To meet the clinical demand, a simple, low-background, ultra-sensitive UDG activity detection method is urgently needed.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yuanyuan Yue, Songtao Wang, Qiang Jin, Nan An, Lina Wu, He Huang
Summary: This article presents a CRISPR/Cas12a-based electrochemical biosensor (E-CRISPR) for detecting lead ions (Pb2+), which utilizes GR-5 DNAzyme as a signal medium to convert Pb2+ into nucleic acid signals and achieves cooperative signal amplification by activating CRISPR/Cas12a cleavage of the electrochemical signal probe, enabling ultrasensitive Pb2+ detection.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Yuanyi Deng, Gaihua Cao, Xiaolong Chen, Mei Yang, Danqun Huo, Changjun Hou
Summary: A fluorescent biosensor combining cascaded strand displacement amplification and CRISPR/Cas12a trans-cleavage ability was established for ultra-sensitive detection of low abundance gene-PIK3CA(H1047R) mutation in breast cancer. The biosensor showed improved sensitivity compared to previous methods, achieving detection of gene-PIK3CA(H1047R) mutation as low as 0.001% in human serum samples. This proposed biosensor has potential applications in low-abundance gene-PIK3CA(H1047R) mutation detection.
Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: Fluorescence biosensing based on CRISPR/Cas12a system with ratiometric probe as the reporter exhibits higher sensitivity compared to traditional TaqMan probe, and it can distinguish and avoid false positive signals.
Article
Chemistry, Analytical
Zhihua Zhou, Zhang Liu, Heng Zhang, Chunxiang Li, Keqin Deng
Summary: A sensitive microRNA detection strategy was designed using a two-stage signal amplification method. By triggering cyclic strand displacement amplification and Cas12a amplification system through 'P-type hybridization, high sensitivity and single-base specificity were achieved. An innovative fluorescence signal readout method was also introduced, significantly improving the detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Qing Liu, Jingjian Liu, Na He, Moli Zhang, Lun Wu, Xiyu Chen, Jun Zhu, Fengying Ran, Qinhua Chen, Hua Zhang
Summary: This study develops a fluorescent biosensor based on magnetic nanoparticles, cascade strand displacement reaction, and CRISPR/Cas12a for the detection of exosomal miR-21 in blood samples of lung cancer patients. The biosensor exhibits high sensitivity and specificity, and can successfully differentiate between lung cancer patients and healthy individuals.
Article
Chemistry, Multidisciplinary
Yong Chen, Yixin Mei, Xingyu Jiang
Summary: This study presents a novel SNP detection method using the CRISPR system to improve universality, sensitivity, and specificity, successfully applied in clinical blood samples. This genotyping method can rapidly detect multiple genotypes in a short period of time.
Article
Food Science & Technology
Ruiqiu Fang, Xiaolong Chen, Xianwen Zhang, Bin Wang
Summary: Harvest duration and postharvest shelf life are important traits in sweet corn, and their regulatory mechanisms are not well understood. In this study, we conducted a comparative transcriptome analysis of sweet corn varieties with different harvest durations and shelf lives. We found that ROS metabolism in kernels was associated with harvest duration and shelf life, and identified several genes involved in this process. These findings provide new insights into the regulatory mechanism of post-harvest shelf life in sweet corn and offer potential strategies for extending its harvest duration and shelf life.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Forestry
Chen Chen, Yilin Weng, Kangting Huang, Xiaolong Chen, Hui Li, Yabin Tang, Lingyue Zhu, Jiachen Wang, Junyu Zhao, Lijun Chen, Lichao Wu, Chunjun Xie, Jian Tang
Summary: Retained residues in high-intensity management eucalypt plantations can contribute significant amounts of nutrients to the soil, but the effects of different residue treatments on decomposition are unclear. This study investigated residue decomposition and nutrient release under different treatments in a Eucalyptus plantation in China. Results showed that decomposition rates varied with residue type and treatment, and decomposition of leaves was faster than that of twigs and branches. Nutrient release was faster in treatments where residues were retained and spread evenly on the soil surface. Appropriate fertilization is still needed in Eucalyptus plantations.
FOREST ECOLOGY AND MANAGEMENT
(2023)
Article
Automation & Control Systems
Siyi Chen, Xinhao Du, Ji Zhao, Huixian Huang, Xiaolong Chen
Summary: This paper proposes a aspect-level sentiment classification approach by combining convolutional neural network (CNN) and proximity-weighted convolution network (PWCN), as well as a new method to calculate the proximity weight. The proposed approach can extract both global and local information from the context, considering syntactic dependencies, and outperforms existing methods on the SemEval 2014 benchmark dataset.
MEASUREMENT & CONTROL
(2023)
Article
Energy & Fuels
Guosheng Yang, Jiaqi Zhang, Hao Zhang, Congbo Wang, Yujia Zhu, Xiaolong Chen
Summary: This paper investigates the impact of a photovoltaic energy storage system on fault current characteristics under low voltage ride-through (LVRT) requirements. The charge-discharge characteristics of the energy storage system are combined with the photovoltaic generation system to enhance LVRT capability. The fault current characteristics of the photovoltaic-energy storage system are discussed based on the inverter control strategy and specific LVRT requirements. Factors influencing the performance of the transformer differential protection are analyzed, including the rated capacity of the photovoltaic-energy storage system, fault severity, and transmission line length.
FRONTIERS IN ENERGY RESEARCH
(2023)
Article
Biodiversity Conservation
Xiaolong Chen, Zhe Yu, Qianbin Di, Hongyu Wu
Summary: This study utilized three-stage data envelopment analysis (DEA) and Tobit regression models to comprehensively evaluate and analyze the performance, trends, and differences of marine ecological welfare (MEW) in China. The findings revealed that the three-stage DEA model outperformed the traditional model by effectively eliminating random errors and external environmental factors while underestimating coastal MEW performance in the country. The southern marine economic circle exhibited overall improvement, while the overall northern circle showed limited progress, and the eastern circle remained stable. External environmental factors significantly influenced MEW performance, and the average performance of each province varied under the influence of slack variables in resource and environmental consumption inputs. Other significant factors affecting China's coastal MEW performance included the level of economic development on land and government environmental regulations, both of which demonstrated a positive correlation. Additionally, insufficient momentum in marine research and technology or low achievement transformation rates had a negative impact. Countermeasures and recommendations based on empirical analysis were also proposed.
ECOLOGICAL INDICATORS
(2023)
Article
Environmental Sciences
Zhe Yu, Xiaolong Chen, Qianbin Di
Summary: This study evaluates the development level, evolution process, and development trend of a sea-land-integrated ecological economy in cities around the Bohai Sea from 2009 to 2019. The results show that the development level generally increased, but varied significantly between cities. In terms of spatial distribution, three major agglomerations were identified, with Dalian, Yantai, Qingdao, Tianjin, and Tangshan as the core cities.
Article
Computer Science, Artificial Intelligence
Guifu Ma, Xiaowei Wang, Manjiang Hu, Wenjie Ouyang, Xiaolong Chen, Yang Li
Summary: This paper proposes a vehicular-cloud-assisted MEC network in urban cities to fully utilize idle resources and ensure task queue stability. Deep neural network is used to predict vehicle trajectory and create vehicular cloud, and a vehicular-cloud-assisted offloading scheme is designed to maximize task throughput. Lyapunov optimization combined with deep reinforcement learning is applied to address the coupling among multiple variables and different time slots.
IEEE TRANSACTIONS ON INTELLIGENT VEHICLES
(2023)
Article
Engineering, Electrical & Electronic
Xiaolong Chen, Ning Zhou, Yongli Li, Bin Li, Botong Li, Jiashuo Liu
Summary: In a HVDC grid, hybrid DC circuit breakers (HCBs) are effective for interrupting fault currents, but conventional HCBs with expensive IGBTs are costly and require extensive implementation. The use of overhead lines raises the likelihood of temporary faults in the grid. This article proposes a cost-effective and adaptive multiport HCB (MHCB) that utilizes capacitor voltage to lower the operating voltage of the MOV and achieves adaptive reclosing based on current amplitude differences.
ELECTRIC POWER SYSTEMS RESEARCH
(2023)
Article
Engineering, Electrical & Electronic
Yuchen Zhu, Yongli Li, Tao Li, Ningning Liu, Xiaolong Chen
Summary: The hybrid cascaded LCC/MMC high voltage direct current (HC-HVDC) system is a promising topology that can overcome the commutation failure problem of LCC-HVDC and ensure efficient power transmission. However, the complex topology poses challenges in fault detection and current limiting due to varying DC fault characteristics at the receiving end. This paper investigates the fault characteristics of the HC-HVDC system and proposes a fault current limiting strategy based on an equivalent RL circuit model and an active sorting algorithm. The proposed strategy achieves fault current suppression, reactive power support, DC voltage stabilization, and energy loss reduction simultaneously.
INTERNATIONAL JOURNAL OF ELECTRICAL POWER & ENERGY SYSTEMS
(2023)
Article
Computer Science, Artificial Intelligence
Yu Du, Jun-qing Li, Xiao-long Chen, Pei-yong Duan, Quan-ke Pan
Summary: This study introduces a hybrid multi-objective optimization algorithm to solve a flexible job shop scheduling problem with time-of-use electricity price constraint, involving machine processing speed, setup time, idle time, and the transportation time between machines. The algorithm combines estimation of distribution algorithm and deep Q-network, with two knowledge-based initialization strategies designed for better performance.
IEEE TRANSACTIONS ON EMERGING TOPICS IN COMPUTATIONAL INTELLIGENCE
(2023)
Article
Chemistry, Analytical
Xiaolong Chen, Chaowang Huang, Qiao Hu, Jing Zhang, Dan Wang, Qianyi You, Mingdong Hu
Summary: The CRISPR/Cas12a system using the screened 2-AP probe as a reporter is more sensitive than the system using ssDNA-FQ probe, allowing detection of ssDNA at a concentration as low as 10(-11) M. Furthermore, the combination of PCR and the 2-AP probe-mediated CRISPR/Cas12a system can detect goat pox virus at a level 10 times lower than the combination of PCR and ssDNA-FQ-mediated system.
Article
Geosciences, Multidisciplinary
Peng-liang Yu, Ting Qu, Ri-zheng He, Jian-li Liu, Su-fen Wang, Xiao-long Chen
Summary: This study explores the application of high-power tensor controlled-source audiomagnetotellurics (CSAMT) in deep geophysical exploration in plateaus with complex terrain and mining areas with strong interference. The results show that the high-power tensor CSAMT improves the signal-to-noise ratio, describes the anisotropy of deep geological bodies, and can achieve a depth of 3 km. This method has great potential for application in deep geophysical exploration in plateaus and mining areas.
Article
Medicine, Research & Experimental
Qing Wang, Jianqing Zhang, Xiaolong Chen, Fa Sun, Kehua Jiang
Summary: This study explored the renal sources and important regulators for S100A8 and S100A9 in nephrolithiasis. The results showed that M1-polarized macrophages were the primary cell type expressing S100A8 and S100A9 in the kidneys of stone patients. Crystals can promote renal tubular epithelial cells to secrete IL6, which up-regulates the expression of S100A8 and S100A9 in M1 macrophages.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Xianfeng Wang, Shasha Liu, Changjun Hou, Mei Yang, Jingzhou Hou, Suyi Zhang, Danqun Huo
Summary: A colorimetric nanoprobe based on gold nanohexagonals coated silver nanotriangles (AgNTs@AuNHs) has been developed for the ultrasensitive detection of trace Pb2+ and Cu2+ ions. The method utilizes the different etching reactions of Pb2+ and Cu2+ on AgNTs@AuNHs to achieve sensitive and selective detection and quantification of these heavy metal ions. Additionally, the method enables visual detection of Pb2+ and Cu2+ through color changes.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Jun Zhou, Chengyue Zou, Danqun Huo, Chengxiang Chu, Shasha Liu, Mei Yang, Suyi Zhang, Xianfeng Wang, Changjun Hou
Summary: In this study, a novel carbon dot probe was successfully developed for the detection of mercury ions, demonstrating high sensitivity and practical applications. The integration of the probe into a sensing strip allowed for specific detection and quantitative measurement of Hg(II).
ANALYTICAL METHODS
(2023)
