Article
Immunology
Sara H. Mahmoud, Ahmed A. Khalil, Noura M. Abo Shama, Marwa F. El Sayed, Reem A. Soliman, Naglaa M. Hagag, Nahed Yehia, Mahmoud M. Naguib, Abdel-Sattar Arafa, Mohamed A. Ali, Mounir M. El-Safty, Ahmed Mostafa
Summary: Controlling avian influenza viruses (AIVs) is mainly done through culling infected bird flocks or using inactivated vaccines. New avian influenza virus subtypes have been reported in Egypt, calling for an updated vaccine. In this study, three H5Nx vaccines were generated using reverse genetic system, and their immunogenicity and cross-reactivity were assessed. The results showed that rgH5N1_2.3.4.4 vaccine induced high immunogenicity and provided efficient protection against different H5Nx HPAIVs.
Article
Pharmacology & Pharmacy
Maria Victoria Sanchez, Thomas Ebensen, Kai Schulze, Diego Esteban Cargnelutti, Eduardo A. Scodeller, Carlos A. Guzman
Summary: Current influenza vaccines targeting surface glycoproteins suffer from mismatches with circulating strains, necessitating the development of universal vaccines. In this study, a novel mucosal vaccine was developed using recombinant nucleoprotein (rNP) and a TLR2/6 agonist. The adjuvanted vaccine induced enhanced humoral and cellular responses, including increased levels of NP-specific antibodies and improved cellular immune profiles. Furthermore, the novel formulation provided protection against H1N1 A/Puerto Rico/8/1934 virus.
Article
Microbiology
Hongliang Zhang, Saisai Zhao, Haojie Zhang, Yu Shen, Peijun Zhang, Hu Shan, Xiulei Cai
Summary: This study successfully expressed the p30, p54, and p72 proteins encoded by African swine fever virus (ASFV) using the Lactobacillus lactis expression system. Oral immunization of rabbits with recombinant L. lactis resulted in increased levels of serum IgG, intestinal mucosal sIgA, cytokines (IL-4 and INF-gamma), and splenocyte viability, suggesting the potential of these recombinant proteins for the design and development of an ASFV subunit vaccine.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Sushmita Kumari, Jayeshbhai Chaudhari, Qinfeng Huang, Phillip Gauger, Marcelo Nunes De Almeida, Yuying Liang, Hinh Ly, Hiep L. X. Vu
Summary: This study evaluated the feasibility of using rPICV as a viral vector to deliver HA antigen and protect pigs against IAV-S challenge. The results demonstrated that the rPICV-H3 vectored vaccine elicited protective immunity against IAV-S.
Review
Medicine, Research & Experimental
Zachary R. Sia, Matthew S. Miller, Jonathan F. Lovell
Summary: The combination of recombinant biotechnology and nanomedicine techniques shows promise in developing a next-generation flu vaccine with broad protection. Various nanoparticle candidates have been tested, showing protection against different influenza strains, production of antibodies with broad reactivity, and activation of potent T cell responses. The future of influenza vaccines may involve combining recombinant antigens with nanoparticle carriers.
MOLECULAR PHARMACEUTICS
(2021)
Review
Immunology
Mark B. Carascal, Rance Derrick N. Pavon, Windell L. Rivera
Summary: This review discusses the progress and advances in the development of recombinant influenza vaccines (RIVs) in the context of heterosubtypic immunity induction for universal vaccine production. Vaccination is considered the most effective strategy against the influenza virus, and RIVs have shown promising potential as universal vaccine candidates.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Nutrition & Dietetics
Xinqi Chen, Yang Chen, Catherine Stanton, Reynolds Paul Ross, Jianxin Zhao, Wei Chen, Bo Yang
Summary: This study aimed to investigate the dose-response effect of Bifidobacterium breve CCFM683 on relieving psoriasis and its underlying patterns. Specifically, the expression of keratin 16, keratin 17, and involucrin were substantially decreased by specific doses of the probiotic. Moreover, the gut microbiota in mice treated with the probiotic was rebalanced and the concentrations of colonic bile acids were positively correlated with the effectiveness of the strain in relieving psoriasis.
Article
Immunology
Nathaniel M. Lewis, Jessie R. Chung, Timothy M. Uyeki, Lisa Grohskopf, Jill M. Ferdinands, Manish M. Patel
Summary: This study analyzes the comparability of relative vaccine effectiveness across different studies and suggests that this comparability is dependent on the absolute vaccine effectiveness of the comparator vaccine. These findings have implications for the design of influenza vaccine studies and data reporting.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Parasitology
Debora Azevedo Borges, Yara Peluso Cid, Viviane de Sousa Magalhaes, Melina Cardilo Campos Alves, Thais Paes Ferreira, Isabelle Vilela Bonfim, Emily Andressa Santos Lima, Juliana Pereira de Freitas, Fabio Barbour Scott
Summary: The study aimed to determine an effective fluazuron (FLU) dose to control A. sculptum larvae in capybaras. The results showed that FLU caused significant morphological and behavioral changes in the larvae, and the efficacy reached 100% for all treated groups.
PARASITES & VECTORS
(2022)
Article
Microbiology
Shirin Strohmeier, Fatima Amanat, Xueyong Zhu, Meagan McMahon, Meagan E. Deming, Marcela F. Pasetti, Kathleen M. Neuzil, Ian A. Wilson, Florian Krammer
Summary: Current seasonal influenza virus vaccines lack the ability to induce robust immune responses to neuraminidase due to various factors, but by designing stabilized recombinant versions of neuraminidase, a promising vaccine antigen may enhance and broaden protection against seasonal influenza viruses, providing broader coverage and stronger protective immune responses.
Article
Virology
Jin Gao, Laura Klenow, Lisa Parsons, Tahir Malik, Je-Nie Phue, Zhizeng Gao, Stephen G. Withers, John Cipollo, Robert Daniels, Hongquan Wan
Summary: This study compared several rNAs produced by the baculovirus/insect cell system with different tetramerization motifs and NA domains, and found that the combination of tetramerization and NA domains significantly impacts the biochemical, immunogenic, and protective properties of the rNAs. Single-dose immunizations were shown to be more informative for evaluating the immune response and protective efficacy of rNAs.
JOURNAL OF VIROLOGY
(2021)
Article
Pharmacology & Pharmacy
JinHong Jiang, Xin Chang, YaoYan Nie, Lingfei Xu, Long Yang, YaLi Peng, Min Chang
Summary: This study demonstrates the protective role of MOTS-c in experimental inflammatory bowel disease (IBD), where MOTS-c enhances macrophage phagocytosis and suppresses inflammatory response. Furthermore, a new molecule called MP, synthesized by linking (PRR)5 to the C-terminus of MOTS-c, exhibits better penetration into colon epithelium and significantly ameliorates DSS-induced colitis upon oral administration.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Microbiology
Keisuke Suganuma, David D. N'Da, Ken-ichi Watanabe, Yusuke Tanaka, Ehab Mossaad, Afraa Elata, Noboru Inoue, Shin-ichiro Kawazu
Summary: This study aimed to evaluate the in vivo efficacy of nitrofurantoin in treating Animal African trypanosomosis (AAT) caused by Trypanosoma congolense. The results showed that oral administration of nitrofurantoin significantly suppressed parasitemia in infected mice, and higher doses led to higher survival rates and cure rates. Oral nitrofurantoin administration has potential trypanocidal efficacy against T. congolense induced AAT.
Article
Immunology
Do-Young Kim, Yong-Myung Kang, Hyun-Kyu Cho, Seo-jeong Park, Myoung-Heon Lee, Youn-Jeong Lee, Hyun-Mi Kang
Summary: In this study conducted in Korea, a new H9N2 virus strain of the Y280 lineage was evaluated. The vaccine candidate rgHS314 showed strong immunogenicity and protective efficacy in SPF chickens, providing high levels of cross-protective immunity against Y439 and Y280 lineages.
Article
Multidisciplinary Sciences
Daniel Ellis, Julia Lederhofer, Oliver J. Acton, Yaroslav Tsybovsky, Sally Kephart, Christina Yap, Rebecca A. Gillespie, Adrian Creanga, Audrey Olshefsky, Tyler Stephens, Deleah Pettie, Michael Murphy, Claire Sydeman, Maggie Ahlrichs, Sidney Chan, Andrew J. Borst, Young-Jun Park, Kelly K. Lee, Barney S. Graham, David Veesler, Neil P. King, Masaru Kanekiyo
Summary: This study reveals the conformational stability of influenza virus neuraminidase (NA) and demonstrates how recombinant NA antigens can be strengthened through structure-based design.
NATURE COMMUNICATIONS
(2022)