标题
Design and analysis of CRISPR–Cas experiments
作者
关键词
-
出版物
NATURE BIOTECHNOLOGY
Volume -, Issue -, Pages -
出版商
Springer Science and Business Media LLC
发表日期
2020-04-14
DOI
10.1038/s41587-020-0490-7
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute
- (2019) Binbin Wang et al. Nature Protocols
- CRISPResso2 provides accurate and rapid genome editing sequence analysis
- (2019) Kendell Clement et al. NATURE BIOTECHNOLOGY
- JACKS: joint analysis of CRISPR/Cas9 knockout screens
- (2019) Felicity Allen et al. GENOME RESEARCH
- Beta-binomial modeling of CRISPR pooled screen data identifies target genes with greater sensitivity and fewer false negatives
- (2019) Hyun-Hwan Jeong et al. GENOME RESEARCH
- Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens
- (2019) Fiona M. Behan et al. NATURE
- CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
- (2019) Kornel Labun et al. NUCLEIC ACIDS RESEARCH
- Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair
- (2019) Wei Chen et al. NUCLEIC ACIDS RESEARCH
- Model-based understanding of single-cell CRISPR screening
- (2019) Bin Duan et al. Nature Communications
- Challenges and recommendations to improve the installability and archival stability of omics computational tools
- (2019) Serghei Mangul et al. PLOS BIOLOGY
- Search-and-replace genome editing without double-strand breaks or donor DNA
- (2019) Andrew V. Anzalone et al. NATURE
- Improved design and analysis of CRISPR knockout screens
- (2018) Chen-Hao Chen et al. BIOINFORMATICS
- FlashFry: a fast and flexible tool for large-scale CRISPR target design
- (2018) Aaron McKenna et al. BMC BIOLOGY
- Deep learning improves prediction of CRISPR–Cpf1 guide RNA activity
- (2018) Hui Kwon Kim et al. NATURE BIOTECHNOLOGY
- GeNets: a unified web platform for network-based genomic analyses
- (2018) Taibo Li et al. NATURE METHODS
- Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III
- (2018) Zongliang Gao et al. Molecular Therapy-Nucleic Acids
- Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens
- (2018) Joshua Pan et al. Cell Systems
- Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
- (2018) Jennifer Listgarten et al. Nature Biomedical Engineering
- Engineered CRISPR-Cas9 nuclease with expanded targeting space
- (2018) Hiroshi Nishimasu et al. SCIENCE
- Web-based design and analysis tools for CRISPR base editing
- (2018) Gue-Ho Hwang et al. BMC BIOINFORMATICS
- Target-Specific Precision of CRISPR-Mediated Genome Editing
- (2018) Anob M. Chakrabarti et al. MOLECULAR CELL
- High‐resolution mapping of cancer cell networks using co‐functional interactions
- (2018) Evan A Boyle et al. Molecular Systems Biology
- Predictable and precise template-free CRISPR editing of pathogenic variants
- (2018) Max W. Shen et al. NATURE
- Predicting the mutations generated by repair of Cas9-induced double-strand breaks
- (2018) Felicity Allen et al. NATURE BIOTECHNOLOGY
- The Gene Ontology Resource: 20 years and still GOing strong
- (2018) NUCLEIC ACIDS RESEARCH
- Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
- (2018) Kendall R. Sanson et al. Nature Communications
- Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
- (2017) Xiquan Liang et al. JOURNAL OF BIOTECHNOLOGY
- Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
- (2017) Nicole M. Gaudelli et al. NATURE
- Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens
- (2017) Fadi J Najm et al. NATURE BIOTECHNOLOGY
- Engineered Cpf1 variants with altered PAM specificities
- (2017) Linyi Gao et al. NATURE BIOTECHNOLOGY
- Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells
- (2017) Robin M Meyers et al. NATURE GENETICS
- GUIDES: sgRNA design for loss-of-function screens
- (2017) Joshua A Meier et al. NATURE METHODS
- Combinatorial CRISPR–Cas9 screens for de novo mapping of genetic interactions
- (2017) John Paul Shen et al. NATURE METHODS
- Am I ready for CRISPR? A user's guide to genetic screens
- (2017) John G. Doench NATURE REVIEWS GENETICS
- A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells
- (2017) Mengjun Tu et al. NUCLEIC ACIDS RESEARCH
- PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens
- (2017) Philipp N. Spahn et al. Scientific Reports
- Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
- (2017) Traver Hart et al. G3-Genes Genomes Genetics
- Ten Simple Rules for Developing Usable Software in Computational Biology
- (2017) Markus List et al. PLoS Computational Biology
- Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
- (2017) Carlos Pulido-Quetglas et al. PLoS Computational Biology
- PPI4DOCK: large scale assessment of the use of homology models in free docking over more than 1000 realistic targets
- (2016) Jinchao Yu et al. BIOINFORMATICS
- Cas-analyzer: an online tool for assessing genome editing results using NGS data
- (2016) Jeongbin Park et al. BIOINFORMATICS
- BAGEL: a computational framework for identifying essential genes from pooled library screens
- (2016) Traver Hart et al. BMC BIOINFORMATICS
- Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
- (2016) Atray Dixit et al. CELL
- CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation and Fucosylation in Bacterial Type III Secretion System-Mediated Cytotoxicity
- (2016) Carlos J. Blondel et al. Cell Host & Microbe
- DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks
- (2016) Megan van Overbeek et al. MOLECULAR CELL
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
- (2016) Alexis C. Komor et al. NATURE
- Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
- (2016) John G Doench et al. NATURE BIOTECHNOLOGY
- Impact of outdated gene annotations on pathway enrichment analysis
- (2016) Lina Wadi et al. NATURE METHODS
- In vivo high-throughput profiling of CRISPR–Cpf1 activity
- (2016) Hui K Kim et al. NATURE METHODS
- Genenames.org: the HGNC and VGNC resources in 2017
- (2016) Bethan Yates et al. NUCLEIC ACIDS RESEARCH
- Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression
- (2016) Aliaksandra Radzisheuskaya et al. NUCLEIC ACIDS RESEARCH
- The STRING database in 2017: quality-controlled protein–protein association networks, made broadly accessible
- (2016) Damian Szklarczyk et al. NUCLEIC ACIDS RESEARCH
- CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions
- (2016) D. M. Munoz et al. Cancer Discovery
- Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
- (2016) A. J. Aguirre et al. Cancer Discovery
- Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
- (2016) Max A Horlbeck et al. eLife
- Quantification of designer nuclease induced mutation rates: a direct comparison of different methods
- (2016) Eric Ehrke-Schulz et al. Molecular Therapy-Methods & Clinical Development
- BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment
- (2016) Annekatrien Boel et al. Scientific Reports
- High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
- (2015) Traver Hart et al. CELL
- Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
- (2015) Bernd Zetsche et al. CELL
- Sequence determinants of improved CRISPR sgRNA design
- (2015) Han Xu et al. GENOME RESEARCH
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities
- (2015) Benjamin P. Kleinstiver et al. NATURE
- CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
- (2015) Miguel A Moreno-Mateos et al. NATURE METHODS
- Identification and characterization of essential genes in the human genome
- (2015) T. Wang et al. SCIENCE
- The Molecular Signatures Database Hallmark Gene Set Collection
- (2015) Arthur Liberzon et al. Cell Systems
- CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool
- (2015) Manuel Stemmer et al. PLoS One
- Tissue-aware data integration approach for the inference of pathway interactions in metazoan organisms
- (2014) Christopher Y. Park et al. BIOINFORMATICS
- Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
- (2014) S. Bae et al. BIOINFORMATICS
- Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
- (2014) Luke A. Gilbert et al. CELL
- Measuring error rates in genomic perturbation screens: gold standards for human functional genomics
- (2014) T. Hart et al. Molecular Systems Biology
- Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
- (2014) John G Doench et al. NATURE BIOTECHNOLOGY
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- Microhomology-based choice of Cas9 nuclease target sites
- (2014) Sangsu Bae et al. NATURE METHODS
- E-CRISP: fast CRISPR target site identification
- (2014) Florian Heigwer et al. NATURE METHODS
- HiTSelect: a comprehensive tool for high-complexity-pooled screen analysis
- (2014) Aaron A. Diaz et al. NUCLEIC ACIDS RESEARCH
- Easy quantitative assessment of genome editing by sequence trace decomposition
- (2014) Eva K. Brinkman et al. NUCLEIC ACIDS RESEARCH
- Targeted genomic rearrangements using CRISPR/Cas technology
- (2014) Peter S. Choi et al. Nature Communications
- DNA targeting specificity of RNA-guided Cas9 nucleases
- (2013) Patrick D Hsu et al. NATURE BIOTECHNOLOGY
- The Genotype-Tissue Expression (GTEx) project
- (2013) John Lonsdale et al. NATURE GENETICS
- Optimization of scarless human stem cell genome editing
- (2013) Luhan Yang et al. NUCLEIC ACIDS RESEARCH
- ClinVar: public archive of relationships among sequence variation and human phenotype
- (2013) Melissa J. Landrum et al. NUCLEIC ACIDS RESEARCH
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- RNA-programmed genome editing in human cells
- (2013) Martin Jinek et al. eLife
- The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
- (2012) Jordi Barretina et al. NATURE
- APPRIS: annotation of principal and alternative splice isoforms
- (2012) Jose Manuel Rodriguez et al. NUCLEIC ACIDS RESEARCH
- Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
- (2012) G. Gasiunas et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- The consensus coding sequence (CCDS) project: Identifying a common protein-coding gene set for the human and mouse genomes
- (2009) K. D. Pruitt et al. GENOME RESEARCH
- Highly parallel identification of essential genes in cancer cells
- (2008) B. Luo et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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