Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Challenges and tackles in metabolic engineering for microbial production of carotenoids

(2019) Chonglong Wang et al.   Microbial Cell Factories

Towards a fully automated algorithm driven platform for biosystems design

(2019) Mohammad HamediRad et al.   Nature Communications

Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineeredEscherichia coli

(2019) Christina V. Dinh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced production of biosynthesized lycopene via heterogenous MVA pathway based on chromosomal multiple position integration strategy plus plasmid systems in Escherichia coli

(2018) Yanlong Wei et al.   BIORESOURCE TECHNOLOGY

Techniques for chromosomal integration and expression optimization in Escherichia coli

(2018) Bing-ming Ou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Toward industrial production of isoprenoids in Escherichia coli : Lessons learned from CRISPR-Cas9 based optimization of a chromosomally integrated mevalonate pathway

(2018) Jorge Alonso-Gutierrez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Escherichia coli as a host for metabolic engineering

(2018) Sammy Pontrelli et al.   METABOLIC ENGINEERING

Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli

(2018) Kevin W. George et al.   METABOLIC ENGINEERING

Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing Bacillus subtilis

(2018) Guanglu Wang et al.   METABOLIC ENGINEERING

Production and extraction of carotenoids produced by microorganisms

(2018) Cassamo Ussemane Mussagy et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli

(2018) Yanjun Li et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Microbial platforms to produce commercially vital carotenoids at industrial scale: an updated review of critical issues

(2018) Ramesh Kumar Saini et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Two-step pathway for isoprenoid synthesis

(2018) Alkiviadis Orfefs Chatzivasileiou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

(2016) Mario Juhas et al.   Microbial Cell Factories

RecA: Regulation and Mechanism of a Molecular Search Engine

(2016) Jason C. Bell et al.   TRENDS IN BIOCHEMICAL SCIENCES

Construction of plasmid-free Escherichia coli for the production of arabitol-free xylitol from corncob hemicellulosic hydrolysate

(2016) Buli Su et al.   Scientific Reports

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Efficient production of xylitol from hemicellulosic hydrolysate using engineered Escherichia coli

(2015) Buli Su et al.   METABOLIC ENGINEERING

Targeted engineering and scale up of lycopene overproduction in Escherichia coli

(2015) Fayin Zhu et al.   PROCESS BIOCHEMISTRY

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

(2015) Gur Pines et al.   ACS Synthetic Biology

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Methylerythritol 4-phosphate (MEP) pathway metabolic regulation

(2014) A. Banerjee et al.   NATURAL PRODUCT REPORTS

Escherichia coli Flagellar Genes as Target Sites for Integration and Expression of Genetic Circuits

(2014) Mario Juhas et al.   PLoS One

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

(2012) Eilon Sharon et al.   NATURE BIOTECHNOLOGY

Site-specific chromosomal integration of large synthetic constructs

(2010) Thomas E. Kuhlman et al.   NUCLEIC ACIDS RESEARCH

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols