Toward industrial production of isoprenoids in Escherichia coli : Lessons learned from CRISPR-Cas9 based optimization of a chromosomally integrated mevalonate pathway

Rewriting yeast central carbon metabolism for industrial isoprenoid production

(2016) Adam L. Meadows et al.   NATURE

Antibiotic resistance in the wild: an eco-evolutionary perspective

(2016) Teppo Hiltunen et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Antibiotic resistance is the quintessential One Health issue

(2016) T. P. Robinson et al.   TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE

CRMAGE: CRISPR Optimized MAGE Recombineering

(2016) Carlotta Ronda et al.   Scientific Reports

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Principal component analysis of proteomics (PCAP) as a tool to direct metabolic engineering

(2015) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Correlation analysis of targeted proteins and metabolites to assess and engineer microbial isopentenol production

(2014) Kevin W. George et al.   BIOTECHNOLOGY AND BIOENGINEERING

Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies

(2014) Ee-Been Goh et al.   METABOLIC ENGINEERING

A brief history of synthetic biology

(2014) D. Ewen Cameron et al.   NATURE REVIEWS MICROBIOLOGY

Chromosome position effects on gene expression in Escherichia coli K-12

(2014) Jack A. Bryant et al.   NUCLEIC ACIDS RESEARCH

Technoeconomic analysis of renewable aviation fuel from microalgae,Pongamia pinnata, and sugarcane

(2013) Daniel Klein-Marcuschamer et al.   Biofuels Bioproducts & Biorefining-Biofpr

Genome-scale genetic engineering in Escherichia coli

(2013) Jaehwan Jeong et al.   BIOTECHNOLOGY ADVANCES

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

(2013) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Chromosome Replication and Segregation in Bacteria

(2012) Rodrigo Reyes-Lamothe et al.   Annual Review of Genetics

Synthesis: A constructive debate

(2012) Jay D. Keasling et al.   NATURE

Genome-scale promoter engineering by coselection MAGE

(2012) Harris H Wang et al.   NATURE METHODS

Regulatory consequences of gene translocation in bacteria

(2012) Dena H. S. Block et al.   NUCLEIC ACIDS RESEARCH

Modular control of multiple pathways using engineered orthogonal T7 polymerases

(2012) Karsten Temme et al.   NUCLEIC ACIDS RESEARCH

Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca

(2012) K. Temme et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A convenient method for multiple insertions of desired genes into target loci on the Escherichia coli chromosome

(2011) Daisuke Koma et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains

(2011) Michele Bruschi et al.   BIOTECHNOLOGY ADVANCES

The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli

(2011) Colin T Archer et al.   BMC GENOMICS

Industrial fermentation of renewable diesel fuels

(2011) Patrick J Westfall et al.   CURRENT OPINION IN BIOTECHNOLOGY

Targeted proteomics for metabolic pathway optimization: Application to terpene production

(2011) Alyssa M. Redding-Johanson et al.   METABOLIC ENGINEERING

Circuitry linking the Csr and stringent response global regulatory systems

(2011) Adrianne N. Edwards et al.   MOLECULAR MICROBIOLOGY

Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals

(2011) Clementina Dellomonaco et al.   NATURE

Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol

(2011) Harry Yim et al.   Nature Chemical Biology

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli

(2011) G. Bokinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for l-threonine production

(2010) Jeong Wook Lee et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Lambda Red Recombineering in Escherichia coli Occurs Through a Fully Single-Stranded Intermediate

(2010) J. A. Mosberg et al.   GENETICS

Evolution in Regulatory Regions Rapidly Compensates the Cost of Nonoptimal Codon Usage

(2010) D. Amoros-Moya et al.   MOLECULAR BIOLOGY AND EVOLUTION

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass

(2010) Eric J. Steen et al.   NATURE

Site-specific chromosomal integration of large synthetic constructs

(2010) Thomas E. Kuhlman et al.   NUCLEIC ACIDS RESEARCH

Manufacturing Molecules Through Metabolic Engineering

(2010) J. D. Keasling   SCIENCE

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Microbial Biosynthesis of Alkanes

(2010) A. Schirmer et al.   SCIENCE

BglBricks: A flexible standard for biological part assembly

(2010) J Christopher Anderson et al.   Journal of Biological Engineering

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Stabilized gene duplication enables long-term selection-free heterologous pathway expression

(2009) Keith E J Tyo et al.   NATURE BIOTECHNOLOGY

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli

(2009) Hiroko Tsuruta et al.   PLoS One

An environmental life cycle assessment comparing Australian sugarcane with US corn and UK sugar beet as producers of sugars for fermentation

(2008) M.A. Renouf et al.   BIOMASS & BIOENERGY

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

(2008) Shota Atsumi et al.   NATURE