4.6 Article

Sol-gel derived strontium-doped SiO2-CaO-MgO-P2O5 bioceramics for faster growth of bone like hydroxyapatite and their in vitro study for orthopedic applications

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MATERIALS CHEMISTRY AND PHYSICS
卷 245, 期 -, 页码 -

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ELSEVIER SCIENCE SA
DOI: 10.1016/j.matchemphys.2020.122763

关键词

Bioactivity; Strontium doping; Osteoblasts like cells; Anti-oxidant properties; MG-63 cells

资金

  1. UGC, New Delhi, India [2012-13/RGNF2012-13-SC-PUN-18654]

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The present study is intended to investigate the influence of SrO doping in SiO2-CaO-MgO-P2O5 (varying SrO as 0, 1, 3 & 5 mol %) sol-gel derived bioceramics. The impact of strontium doping on structural and biological properties is assessed using X-ray diffraction (XRD) analysis, Fourier transform infrared (FTIR) spectra, degradability in simulated body fluid (SBF), Brunauer-Emmett- Teller (BET), Barret-Joyner-Halenda (BJH) and anti-oxidant activity against hydrogen peroxide (H2O2) induced oxidative stress using cell culture studies. Field emission scanning electron microscopy with energy dispersive X-ray (FESEMM-EDX) technique is used for morphological studies. The in vitro tests demonstrate that incorporation of strontium does not hinder the precipitation of the hydroxyapatite. The Zeta potential studies reveal that samples possessed negative surface charge, which might promote the binding and augmentation of osteoblasts. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell proliferation assay conducted on human osteosarcoma MG-63 cell line indicate significant cell survival. With regard to the antioxidant properties, samples with the highest concentration of strontium exhibits maximum protection against hydrogen-induced oxidative stress in cell culture. Thus, our studies suggest that SrO doped bioceremics demonstrate excellent physical and biological properties and strongly present their candidature as successful implant materials.

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