Article
Immunology
Sofya A. Kust, Maria A. Streltsova, Alexander V. Panteleev, Natalya L. Karpina, Irina V. Lyadova, Alexander M. Sapozhnikov, Elena I. Kovalenko
Summary: NK cells expressing HLA-DR play a critical role in the control of tuberculosis infection by exhibiting higher proliferative activity and increased expression of NK receptors. These cells show higher IFN-γ production and degranulation level in response to mycobacteria stimulation, serving as an important link to the adaptive immunity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemical Research Methods
Liran Komov, Dganit Melamed Kadosh, Eilon Barnea, Arie Admon
Summary: This study found that interferons can promote the rapid degradation of defective ribosomal products (DRiPs), leading to a change in the proportion of DRiP-derived MHC peptides, which is crucial for combating viral infections.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Immunology
Maja Houseman, Melody Ying-Yu Huang, Markus Huber, Matthias Staiger, Lan Zhang, Anneliese Hoffmann, Christoph Lippuner, Frank Stueber
Summary: HLA-DR is a cell-surface receptor that plays a key role in immune responses. This study identified potential microRNA regulators of HLA-DR expression and investigated the underlying gene regulations. The results provide insights into the role of microRNAs in HLA-DR regulation and may have potential clinical applications.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Editorial Material
Immunology
Antonius A. de Waard, Tamara Verkerk, Marlieke L. M. Jongsma, Kelly Hoefakker, Sunesh Sethumadhavan, Carolin Gerke, Sophie Bliss, Xiangrui Kong, George M. C. Janssen, Arnoud H. de Ru, Frans H. J. Claas, Arend Mulder, Robert Tampe, Peter A. van Veelen, Anne Halenius, Robbert M. Spaapen
Summary: PAKC is a novel panel of ten cell lines that have individual components of the HLA class I antigen presentation pathway knocked out, providing a single model system for studies across multiple areas. This system is expected to accelerate research in oncology, infectiology, and autoimmunity.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Review
Immunology
Karthik Dhatchinamoorthy, Jeff D. Colbert, Kenneth L. Rock
Summary: Loss of MHC I antigen presentation is common in many cancers, which may impair immune responses and affect the efficacy of immunotherapy. Studies have discussed underlying mechanisms through which some cancers evade immune killing by shutting down the MHC I pathway, and proposed potential strategies to overcome this limitation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Rheumatology
Shuyang Zhao, Ricardo Grieshaber-Bouyer, Deepak A. Rao, Philipp Kolb, Haizhang Chen, Ivana Andreeva, Theresa Tretter, Hanns-Martin Lorenz, Carsten Watzl, Guido Wabnitz, Lars-Oliver Tykocinski, Wolfgang Merkt
Summary: Expanded HLA-DR+CD90+ synovial fibroblasts in patients with rheumatoid arthritis (RA) play a key role in inflammation. Activated NK cells induced the expression of HLA-DR on CD90+ synovial fibroblasts through IFNγ, leading to an inflammatory phenotype. JAK inhibition can target these proinflammatory synovial fibroblasts.
ARTHRITIS & RHEUMATOLOGY
(2022)
Article
Oncology
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijing Wei, Zhenhai Tang, Guo Chen, Bing Wei, Yunxia Cao
Summary: In this study, the researchers found that upregulated autophagy and decreased expression of MHC-I and NLRC5 were associated with endometrial cancer (EC). Inhibition of autophagy was found to suppress MHC-I gene expression. They also discovered that LC3 interacted with NLRC5 to inhibit the NLRC5-mediated MHC-I antigen presentation pathway. These findings suggest that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy for EC management.
Article
Immunology
Paula Ruibal, Ian Derksen, Marjolein van Wolfswinkel, Linda Voogd, Kees L. M. C. Franken, Angela F. El Hebieshy, Thorbald van Hall, Tom A. W. Schoufour, Ruud H. Wijdeven, Tom H. M. Ottenhoff, Ferenc A. Scheeren, Simone A. Joosten
Summary: There is growing interest in HLA-E-restricted T-cell responses as a potential vaccination target. This study developed a method for efficient peptide thermal exchange on HLA-E monomers and multimers, allowing high-throughput production. The optimized conditions for detection and analysis of peptide-specific T cells revealed new insights into the interaction between HLA-E and NKG2A/CD94 receptors.
Article
Nutrition & Dietetics
Sheng-Jie Shiue, Chao-Ling Cheng, Han-Shiang Shiue, Chun-Nan Chen, Sheng-Wei Cheng, Li-Wei Wu, Ganbolor Jargalsaikhan, Tze-Sian Chan, Hsin-Yi Lin, Ming-Shun Wu
Summary: This study found that oral Arthrospira as an add-on therapy can reduce HBsAg levels in CHB patients and improve immune function by promoting the activation of B cells, T cells, and NK cells.
Review
Immunology
Gulce Sari, Kenneth Rock
Summary: CD8 T cells recognize cancers through antigenic peptides presented on a tumor's surface MHC-I molecules. Many cancers, by inactivating MHC-I antigen presentation, evade control by CD8 T cells. This review discusses recent findings on the various mechanisms that cancers employ to impair antigen presentation, the consequences of these changes, and the potential for reversing these defects in some cases.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Patrick C. Lee, Susan Klaeger, Phuong M. Le, Keegan Korthauer, Jingwei Cheng, Varsha Ananthapadmanabhan, Thomas C. Frost, Jonathan D. Stevens, Alan Y. L. Wong, J. Bryan Iorgulescu, Anna Y. Tarren, Vipheaviny A. Chea, Isabel P. Carulli, Camilla K. Lemvigh, Christina B. Pedersen, Ashley K. Gartin, Siranush Sarkizova, Kyle T. Wright, Letitia W. Li, Jason Nomburg, Shuqiang Li, Teddy Huang, Xiaoxi Liu, Lucas Pomerance, Laura M. Doherty, Annie M. Apffel, Luke J. Wallace, Suzanna Rachimi, Kristen D. Felt, Jacquelyn O. Wolff, Elizabeth Witten, Wandi Zhang, Donna Neuberg, William J. Lane, Guanglan Zhang, Lars R. Olsen, Manisha Thakuria, Scott J. Rodig, Karl R. Clauser, Gabriel J. Starrett, John G. Doench, Sara J. Buhrlage, Steven A. Carr, James A. DeCaprio, Catherine J. Wu, Derin B. Keskin
Summary: This study reveals transcriptional suppression of HLA-I antigen presentation in Merkel cell carcinoma and identifies MYCL, PRC1.1, and USP7 as regulators in this process.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Genetics & Heredity
Esteban Arrieta-Bolanos, Diana Iraiz Hernandez-Zaragoza, Rodrigo Barquera
Summary: HLA frequencies show variation across human populations. Geography has a strong effect on the distribution of HLA class I allele groups, while class II frequencies correlate less clearly to actual geographic location. The study also reveals intra-continental sub-clusters and characteristic HLA allele group patterns.
FRONTIERS IN GENETICS
(2023)
Review
Immunology
Karolina D. Witt
Summary: MHC class I antigen processing is a less recognized area in nonviral host-pathogen interactions, involving immunology and cell biology. This review focuses on the MHC-I antigen processing pathway and alternative sources of antigens, particularly in the context of Mycobacterium tuberculosis (Mtb) as an intracellular pathogen. It explores how Mtb manipulates host immunity for survival and proposes directions for MHC-I-focused approaches in developing vaccines against tuberculosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Maria Kalomoiri, Chandana Rao Prakash, Sonja Lagstroem, Kai Hauschulz, Ewoud Ewing, Klementy Shchetynsky, Lara Kular, Maria Needhamsen, Maja Jagodic
Summary: The Human Leukocyte Antigen (HLA) locus is associated with various complex diseases, especially autoimmune and inflammatory conditions. The HLA-DR15 haplotype, for example, is a major risk factor for developing Multiple Sclerosis in Caucasians, suggesting its important role in the etiology of this chronic inflammatory disease. Recent studies indicate that the levels of HLA molecule expression, which are epigenetically controlled, also contribute to disease development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Julia D. Vavilova, Anna A. Boyko, Natalya Troyanova, Natalya Ponomareva, Vitaly F. Fokin, Ekaterina Y. Fedotova, Maria A. Streltsova, Sofya A. Kust, Maria Grechikhina, Olga A. Shustova, Tatyana L. Azhikina, Elena Kovalenko, Alexander M. Sapozhnikov
Summary: Parkinson's disease is a proteostasis disorder caused by the accumulation of alpha-synuclein in a specific brain region. This study examined the changes in chaperones and p62 protein levels in peripheral blood mononuclear cells (PBMC) of PD patients. While there were no changes in the intracellular HSP70 protein pool, increased transcriptional activity of stress-associated genes and elevated p62 levels were observed. The combination of HSPA6 and p62 markers showed diagnostic significance with intermediate sensitivity and high specificity for PD patients.
Article
Biochemistry & Molecular Biology
Maria O. Ustiuzhanina, Julia D. Vavilova, Anna A. Boyko, Maria A. Streltsova, Sofya A. Kust, Leonid M. Kanevskiy, Alexander M. Sapozhnikov, Rustam N. Iskhakov, Ekaterina O. Gubernatorova, Marina S. Drutskaya, Mikhail V. Bychinin, Oksana A. Zhukova, Oksana N. Novikova, Anna G. Sotnikova, Gaukhar M. Yusubalieva, Vladimir P. Baklaushev, Elena I. Kovalenko
Summary: This study investigated circulating NK cells in COVID-19 patients and focused on their activation, depletion, changes in key receptor surface expression, and functional activity. The results showed that NK cell activation in COVID-19 patients is accompanied by changes in circulating cytokines. The progression of the disease was associated with a decrease in NKG2D(+) and CD16(+) NK cells and an increase in PD-1, indicating NK cell exhaustion. Higher levels of NKG2D(+) NK cells distinguished survivors from non-survivors in the ICU group. Phenotypic changes in circulating NK cells suggest that intense activation during SARS-CoV-2 infection is accompanied by NK cell exhaustion, which may critically impact disease outcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julia D. Vavilova, Maria O. Ustiuzhanina, Anna A. Boyko, Maria A. Streltsova, Sofya A. Kust, Leonid M. Kanevskiy, Rustam N. Iskhakov, Alexander M. Sapozhnikov, Ekaterina O. Gubernatorova, Marina S. Drutskaya, Mikhail V. Bychinin, Oksana N. Novikova, Anna G. Sotnikova, Gaukhar M. Yusubalieva, Vladimir P. Baklaushev, Elena I. Kovalenko
Summary: The activation and differentiation of NKT-like cells and CD56(-) T cells in COVID-19 patients were analyzed. Severe COVID-19 was associated with a decrease in CD8(+) T cells and a shift towards more differentiated cytotoxic CD8(+) T cells. COVID-19 progression was indicated by decreased percentages of NKG2D(+) and NKG2A(+) cells and increased PD-1 and HLA-DR expression levels in both CD56(-) and CD56(+) T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Anna A. Boyko, Maria O. Ustiuzhanina, Julia D. Vavilova, Maria A. Streltsova, Sofya A. Kust, Andrei E. Siniavin, Irina V. Astrakhantseva, Marina S. Drutskaya, Elena I. Kovalenko
Summary: Independent studies and large-scale post-vaccination follow-up studies have demonstrated the highly effective humoral immune response induced by the Sputnik V vaccine. However, the impact of Sputnik V on cell-mediated immunity is still being investigated. This study aimed to assess the activation and inhibitory receptors, as well as activation and proliferative senescence markers in NK and T lymphocytes, after Sputnik V vaccination.
Review
Biochemistry & Molecular Biology
Anastasia I. I. Palamarchuk, Elena I. I. Kovalenko, Maria A. A. Streltsova
Summary: TERT, as a core component of telomerase, is not only responsible for lengthening telomeres, but also involved in multiple signaling pathways with various intracellular localizations and functions. Upregulation of TERT expression and increased telomerase activity in cancer and somatic cells are associated with improved survival and persistence.
Article
Biochemistry & Molecular Biology
Ivan V. Bogdanov, Maria A. Streltsova, Elena I. Kovalenko, Alexander M. Sapozhnikov, Pavel V. Panteleev, Tatiana V. Ovchinnikova
Summary: LL-37 is a human defense antimicrobial peptide that exhibits antimicrobial activities and can induce cytokine production. However, its physiological relevance and absorption in the gut remains unclear.
Article
Biochemistry & Molecular Biology
M. A. Streltsova, A. A. Boyko, M. O. Ustiuzhanina, A. I. Palamarchuk, N. A. Alekseeva, R. A. Velichinskii, J. D. Vavilova, M. V. Grechikhina, A. M. Sapozhnikov, S. M. Deev, E. I. Kovalenko
Summary: This study analyzed the heterogeneity of NK cell subsets subjected to retroviral transduction and found that subsets with KIR2DL2/DL3 and NKG2C markers were more efficiently modified. The modified cells acquired the adaptive NK cell phenotype by expressing CD57 de novo.
DOKLADY BIOCHEMISTRY AND BIOPHYSICS
(2022)