Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Removal of glycerol from enzymatically produced 2-α-d-glucosyl-glycerol by discontinuous diafiltration

(2020) Andreas Kruschitz et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Efficient bioconversion of sucrose to high-value-added glucaric acid by in vitro metabolic engineering

(2019) Huihui Su et al.   ChemSusChem

Multi-enzyme systems and recombinant cells for synthesis of valuable saccharides: Advances and perspectives

(2019) Jiangang Yang et al.   BIOTECHNOLOGY ADVANCES

The EMBL-EBI search and sequence analysis tools APIs in 2019

(2019) Fábio Madeira et al.   NUCLEIC ACIDS RESEARCH

Sucrose 6F-phosphate phosphorylase: a novel insight in the human gut microbiome

(2019) Alexandra S. Tauzin et al.   Microbial Genomics

Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases

(2019) Mamoru Nishimoto   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Structural Comparison of a Promiscuous and a Highly Specific Sucrose 6F-Phosphate Phosphorylase

(2019) Jorick Franceus et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Three‐Enzyme Phosphorylase Cascade for Integrated Production of Short‐Chain Cellodextrins

(2019) Chao Zhong et al.   Biotechnology Journal

Exploring the sequence diversity in glycoside hydrolase family 13_18 reveals a novel glucosylglycerol phosphorylase

(2018) Jorick Franceus et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Discovery of a Kojibiose Phosphorylase in Escherichia coli K-12

(2018) Keya Mukherjee et al.   BIOCHEMISTRY

Reversibility of a Point Mutation Induced Domain Shift: Expanding the Conformational Space of a Sucrose Phosphorylase

(2018) Michael Kraus et al.   Scientific Reports

Biocatalytic Synthesis of the Rare Sugar Kojibiose: Process Scale-Up and Application Testing

(2017) Koen Beerens et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

(2017) Juan M. Bolivar et al.   JOURNAL OF BIOTECHNOLOGY

Natural products from myxobacteria: novel metabolites and bioactivities

(2017) J. Herrmann et al.   NATURAL PRODUCT REPORTS

Converting bulk sugars into prebiotics: semi-rational design of a transglucosylase with controlled selectivity

(2016) Tom Verhaeghe et al.   CHEMICAL COMMUNICATIONS

Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

(2016) M. Kraus et al.   CHEMICAL COMMUNICATIONS

Glucosylglycerate metabolism, bioversatility and mycobacterial survival

(2016) Daniela Nunes-Costa et al.   GLYCOBIOLOGY

Sugar Consumption and Changes in Dental Caries from Childhood to Adolescence

(2016) M.A. Peres et al.   JOURNAL OF DENTAL RESEARCH

Sugar tax could sweeten a market failure

(2016) Adam Briggs   NATURE

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

(2015) Patricia Wildberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Multistep Synthesis of UDP-Glucose Using Tailored, Permeabilized Cells of E. coli

(2015) Christian Weyler et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Diversity of phosphorylases in glycoside hydrolase families

(2015) Motomitsu Kitaoka   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Glycoside phosphorylases: Structure, catalytic properties and biotechnological potential

(2015) Vladimír Puchart   BIOTECHNOLOGY ADVANCES

Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases

(2015) Yuan Liu et al.   CARBOHYDRATE RESEARCH

Redesign of the Active Site of Sucrose Phosphorylase through a Clash-Induced Cascade of Loop Shifts

(2015) Michael Kraus et al.   CHEMBIOCHEM

Sugar consumption, metabolic disease and obesity: The state of the controversy

(2015) Kimber L. Stanhope   CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES

Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization

(2015) Karel De Winter et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Production and application of a rare disaccharide using sucrose phosphorylase from Leuconostoc mesenteroides

(2015) Kenji Morimoto et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

The quest for a thermostable sucrose phosphorylase reveals sucrose 6′-phosphate phosphorylase as a novel specificity

(2014) Tom Verhaeghe et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of α-d-Glucosides Using Sucrose Phosphorylase

(2014) Karel De Winter et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

2-O-α-D-Glucosylglycerol Phosphorylase from Bacillus selenitireducens MLS10 Possessing Hydrolytic Activity on β-D-Glucose 1-Phosphate

(2014) Takanori Nihira et al.   PLoS One

One-Pot Enzymatic Conversion of Sucrose to Synthetic Amylose by using Enzyme Cascades

(2014) Peng Qi et al.   ACS Catalysis

Consensus engineering of sucrose phosphorylase: The outcome reflects the sequence input

(2013) Dirk Aerts et al.   BIOTECHNOLOGY AND BIOENGINEERING

Chiral resolution through stereoselective transglycosylation by sucrose phosphorylase: application to the synthesis of a new biomimetic compatible solute, (R)-2-O-α-d-glucopyranosyl glyceric acid amide

(2013) Patricia Wildberger et al.   CHEMICAL COMMUNICATIONS

Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability

(2013) Karel De Winter et al.   GREEN CHEMISTRY

Discovery of β-1,4-d-Mannosyl-N-acetyl-d-glucosamine Phosphorylase Involved in the Metabolism ofN-Glycans

(2013) Takanori Nihira et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning

(2013) Tom Verhaeghe et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Probing enzyme–substrate interactions at the catalytic subsite ofLeuconostoc mesenteroidessucrose phosphorylase with site-directed mutagenesis: the roles of Asp49and Arg395

(2012) Patricia Wildberger et al.   BIOCATALYSIS AND BIOTRANSFORMATION

Enzymatic Glycosylation of Small Molecules: Challenging Substrates Require Tailored Catalysts

(2012) Tom Desmet et al.   CHEMISTRY-A EUROPEAN JOURNAL

An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity

(2012) Karel De Winter et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Enzymatic glycosyl transfer: mechanisms and applications

(2011) Tom Desmet et al.   BIOCATALYSIS AND BIOTRANSFORMATION

Transglucosylation potential of six sucrose phosphorylases toward different classes of acceptors

(2011) Dirk Aerts et al.   CARBOHYDRATE RESEARCH

Aromatic interactions at the catalytic subsite of sucrose phosphorylase: Their roles in enzymatic glucosyl transfer probed with Phe52→ Ala and Phe52→ Asn mutants

(2011) Patricia Wildberger et al.   FEBS LETTERS

Operational stability of immobilized sucrose phosphorylase: Continuous production of α-glucose-1-phosphate at elevated temperatures

(2011) Karel De Winter et al.   PROCESS BIOCHEMISTRY

Increasing the thermostability of sucrose phosphorylase by a combination of sequence- and structure-based mutagenesis

(2011) An Cerdobbel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Practical Preparation ofD-Galactosyl-β1→4-L-rhamnose Employing the Combined Action of Phosphorylases

(2010) Masahiro NAKAJIMA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Regioselective O-glucosylation by sucrose phosphorylase: a promising route for functional diversification of a range of 1,2-propanediols

(2010) Christiane Luley-Goedl et al.   CARBOHYDRATE RESEARCH

Increasing the thermostability of sucrose phosphorylase by multipoint covalent immobilization

(2010) An Cerdobbel et al.   JOURNAL OF BIOTECHNOLOGY

Regio- and stereoselective glucosylation of diols by sucrose phosphorylase using sucrose or glucose 1-phosphate as glucosyl donor

(2010) Rokus Renirie et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0

(2010) Stéphane Guindon et al.   SYSTEMATIC BIOLOGY

Sucrose phosphorylase: a powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals

(2009) Christiane Goedl et al.   BIOCATALYSIS AND BIOTRANSFORMATION

A High-Yielding Biocatalytic Process for the Production of 2-O-(α-D-glucopyranosyl)-sn-glycerol, a Natural Osmolyte and Useful Moisturizing Ingredient

(2008) Christiane Goedl et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION