Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Thuy-Hang Nguyen, Stephanie Conotte, Alexandra Belayew, Anne-Emilie Decleves, Alexandre Legrand, Alexandra Tassin
Summary: Muscular dystrophies are genetic degenerative muscle disorders characterized by muscle wasting, often accompanied by respiratory impairments and hypoxemia. Hypoxic stress activates compensatory mechanisms, with HIF-1α playing a key role. Alterations in HIF-1α in muscle may impact the pathophysiology of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Liyun Yong, Shasha Tang, Haixin Yu, Hongyi Zhang, Yi Zhang, Yuan Wan, Fengfeng Cai
Summary: Breast cancer is the most common cancer in women, and drug resistance is a persistent clinical challenge. Hypoxia-inducible Factor-1 Alpha (HIF-1α) plays a crucial role in regulating cellular response to hypoxia and is closely associated with multidrug resistance in breast cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Yi Chen, Qian-Long Wang, Wei-Xue Huang, Long-Wei Li, Lan-Cong Liu, Yi Yang, You-Jiao Wu, Shan-Shan Song, Hao Ma, Hua Zhou, Pei Luo
Summary: This study aimed to analyze and evaluate the dynamic changes in HIF-1 alpha concentration in serum exosomes during bacterial peritonitis. A new colorimetric method to quantitate these changes was established. The results showed that the serum exosomal HIF-1 alpha levels increased in the early stage of bacterial peritonitis, reached a peak at 24-48 hours, and then gradually decreased. Intervention treatment reduced the abdominal infection extent, HIF-1 alpha concentration in serum exosomes, and the serum pro-inflammatory factors levels. These findings suggest that serum exosomal HIF-1 alpha can be used as a biomarker for predicting and monitoring the pathological process of bacterial peritonitis.
Review
Cell Biology
Mykyta Malkov, Chee Teik Lee, Cormac T. Taylor
Summary: Hypoxia and inflammation frequently co-occur in inflammatory diseases, with pro-inflammatory cytokines such as TNF-alpha and IL-1 beta directly impacting HIF signaling pathways. Multi-level crosstalk between inflammatory and hypoxic signaling pathways plays a crucial role in shaping the degree of inflammation at hypoxic sites.
Article
Environmental Sciences
Meriam N. N. Rezk, Gerges M. Beshreda, Dalia Abdelrahman Meshref, Walaa Yehia Abdelzaher, Gaber El-Saber Batiha, Amin A. Hafiz, Duaa Althumairy, Nada H. Aljarba, Nermeen N. Welson
Summary: This study aimed to investigate the role of hypoxiainducible factor-1 alpha (HIF-1 alpha) in the diagnosis, prediction, and follow-up of Dormex-intoxicated patients. The results showed that HIF-1 alpha can effectively predict and follow up patients within 24 hours after admission. Additionally, blood-related indicators were also significantly abnormal in patients with Dormex intoxication.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Medicine, Research & Experimental
Mariko Miyashita-Ishiwata, Malak El Sabeh, Lauren D. Reschke, Sadia Afrin, Mostafa A. Borahay
Summary: This study reveals an aberrant response of leiomyoma cells to hypoxia, characterized by increased cell proliferation and secretion of angiogenic factors. This abnormal response may contribute to the development and growth of leiomyoma.
Article
Chemistry, Physical
Zheng Wang, Xuewen Mu, Qian Yang, Jiajia Luo, Yanjun Zhao
Summary: This study utilizes hypoxia-responsive polymer micelles combined with an NQO1 inhibitor (dicoumarol) to enhance the sensitivity of ovarian cancer cells to the anticancer drug sorafenib under low oxygen conditions. Both drugs are encapsulated in nanoscale micelles and the degradation of HIF-1α is increased to improve apoptosis induced by the drug.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kenta Kaihara, Shuji Nakagawa, Yuji Arai, Hiroaki Inoue, Shinji Tsuchida, Yuta Fujii, Yoichiro Kamada, Tsunao Kishida, Osam Mazda, Kenji Takahashi
Summary: The study found that hypoxia can suppress the development of rheumatoid arthritis by controlling HIF-1 alpha expression, delaying joint swelling and reducing joint destruction. Deferoxamine-mediated activation of HIF-1 alpha can counteract the inhibitory effect of sustained hypoxia on pro-inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yu-Kun Jie, Jian-Jun Jiang, Hong-Ling Ma, Chang -Hong Cheng, Guang-Xin Liu, Si-Gang Fan, Juan Feng, Zhi-Xun Guo
Summary: In this study, a novel PHD2 gene, SpPHD2, was cloned and identified in the mud crab Scylla paramamosain. The mRNA expression of SpPHD2 was widely distributed across all examined tissues, and the protein was mainly localized in the cytoplasm. Silencing SpPHD2 in vivo upregulated SpHIF-1 alpha and downstream genes, while overexpression of SpPHD2 in vitro dose-dependently reduced SpHIF-1 alpha transcriptional activity, indicating the crucial role of SpPHD2 in regulating SpHIF-1 alpha.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Review
Oncology
Bich Phuong Bui, Phuong Linh Nguyen, Kyeong Lee, Jungsook Cho
Summary: This review discusses the crucial role of hypoxia-inducible factor-1 (HIF-1) in tumor biology, particularly in hypoxic environments. Despite numerous identified HIF-1 inhibitors as potential therapeutic agents for various cancers, none of them have been successfully translated into clinically available cancer treatments. The review summarizes the roles of the HIF-1 pathway in tumor proliferation, angiogenesis, metastasis, drug resistance, and cancer-related pain. Additionally, it highlights the current status of HIF-1 inhibitors in clinical trials and their future perspectives. HIF-1 inhibitors may be promising combinational therapeutic interventions to improve the efficacy of current cancer treatments and reduce drug resistance and cancer-related pain.
Article
Biochemistry & Molecular Biology
Marina Sakai, Nobuaki Takahashi, Hiroaki Ikeda, Yutaka Furutani, Shoko Higuchi, Takehiro Suzuki, Naoshi Dohmae, Sayaka Kobayashi, Hiroshi Harada, Soichi Kojima, Tomokazu Matsuura, Akira Hattori, Hideaki Kakeya
Summary: HIF-1 is a promising drug target for cancer chemotherapy, with KUSC-5037 identified as an effective inhibitor under hypoxic conditions. KUSC-5037 suppresses HIF-1 signaling and reduces the expression of HIF-1 target genes, showing potential as a lead molecule for cancer treatment.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Cardiac & Cardiovascular Systems
Ka Lin Heck-Swain, Jiwen Li, Wei Ruan, Xiaoyi Yuan, Yanyu Wang, Michael Koeppen, Holger K. Eltzschig
Summary: The myeloid-expressed HIF1A plays a functional role in providing cardioprotection during ischemia and reperfusion injury by inducing the neuronal guidance molecule netrin-1 in neutrophils.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Immunology
Gabriela Burczyk, Iwona Cichon, Elzbieta Kolaczkowska
Summary: 4-octyl itaconate (4-OI) diminishes the formation of neutrophil extracellular traps (NETs) in mice, regardless of their health status or metabolic challenges. The inhibition is mediated by downregulating Hif-1 alpha and inducing HO-1.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Elena V. Mitroshina, Maria O. Savyuk, Evgeni Ponimaskin, Maria V. Vedunova
Summary: Hypoxia is a common pathological condition induced by various events, with the body's adaptation to it being crucial for health and disease. HIFs, a family of transcription factors, play a key role in cellular responses to hypoxia and are increasingly viewed as potential targets for treating a range of hypoxia-associated diseases. The role of HIFs in adaptation to hypoxia is universal across tissue types, including the CNS, where they are involved in regulating neurogenesis, nerve cell differentiation, and neuronal apoptosis, potentially offering new therapeutic opportunities.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Legal
N. Kern, S. Potente, M. A. Verhoff, S. C. Koelzer
Summary: The case illustrates the potential dangers of night storage heaters for unattended infants and toddlers if they are not properly secured and the children are unattended. It also underlines the importance of an interdisciplinary reconstruction, when necessary, including a detailed inspection of the location in order to distinguish a possible accident from child abuse.
Article
Medicine, Legal
M. A. Verhoff, M. F. Klinger, M. Kettner, C. G. Birngruber
Summary: Establishing a biological profile is crucial in identifying unknown individuals in advanced stages of decomposition, as seen in a case where a skeletonized corpse was found in a basement. Conflicting age estimation results were resolved through DNA analysis, leading to the identification of a missing woman. The scene, reminiscent of crime movies, highlighted the challenges and importance of osteological examinations in such cases.
Article
Medicine, Legal
S. Plenzig, F. Holz, M. Kettner, M. A. Verhoff, C. G. Birngruber
Summary: In Frankfurt, the necessity of changing the manner of death based on autopsy findings highlights the need to increase the autopsy rate. Even with forensic experts performing the first postmortem external examinations, special case constellations pose challenges for medical examiners.
Article
Medicine, Legal
T. E. N. Ohlwaerther, F. Holz, F. Heidorn, M. A. Verhoff, C. G. Birngruber
Summary: The study analyzed bone findings in the Institute for Forensic Medicine Giessen from 2005 to 2019, finding that 40% were discovered in forest and meadow areas. Human bones were found in 58% of the cases, with 32% having a forensically relevant postmortem interval. Sign of perimortem violence were found in 6% of human bone findings.
Article
Entomology
Lena Lutz, Marcel A. Verhoff, Tabea Rosenbaum, Jens Amendt
Summary: The study monitored the oviposition activity of necrophagous flies (Calliphoridae, Sarcophagidae) using mouse carcasses in urban and rural habitats in Frankfurt, Germany. The analysis showed that the seasonal adaption, habitat type, and temperature are the most important factors influencing the oviposition behavior and activity of these flies.
ECOLOGICAL ENTOMOLOGY
(2022)
Article
Medicine, Legal
Carl Daniel Lehmann-Leo, Frank Ramsthaler, Christoph G. Birngruber, Marcel A. Verhoff
Summary: The study aims to evaluate the correlations between carotid artery and renal tissue changes and age-at-death in a Western European population. The results show a strong correlation between carotid intima-media thickness and chronological age, as well as a 37.7-43.1% correlation between glomerulosclerotic incidence and predicted age group.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Medicine, Legal
Stefanie Scheiper-Welling, Monika Tabunscik, Theresa E. Gross, Tina Jenewein, Britt M. Beckmann, Constanze Niess, Elise Gradhand, Cora Wunder, Peter M. Schneider, Markus A. Rothschild, Marcel A. Verhoff, Silke Kauferstein
Summary: Sudden cardiac death in young individuals may be caused by inherited arrhythmic syndromes. However, the pathogenicity of the majority of genetic variants remains unclear, posing challenges in genetic investigations. Thus, an experienced multidisciplinary team is needed to interpret variant significance, identify high-risk relatives, and develop appropriate forensic guidelines.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Medicine, Legal
Constantin Lux, Miriam Klinger, Patrick Sauer, Marcel A. Verhoff, Mattias Kettner
Summary: This study evaluates the feasibility of indocyanine green (ICG) as a fluorescent agent for postmortem heart angiography. The basic patterns of findings in ICG fluorescence angiography associated with healthy hearts are presented. The method can be easily integrated into workflow without restricting macroscopic or histologic diagnostics.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Clinical Neurology
Christine Reif-Leonhard, Dorothea Lemke, Franziska Holz, Kira F. Ahrens, Christoph Fehr, Markus Steffens, Michael Grube, Christine M. Freitag, Sarah C. Koelzer, Sabine Schlitt, Rebekka Gebhardt, Theresa Gaedeke, Helga Schmidt, Ferdinand M. Gerlach, Kira Wolff, Michael Staeblein, Nora Hauschild, Inga Beig, Louisa Wagner, Juliane Mueller, Marcel A. Verhoff, Christiane Schlang, Andreas Reif
Summary: This study examines the changes in suicide attempts and completed suicides during the COVID-19 pandemic in Frankfurt/Main. The number of suicide attempts decreased significantly, while the number of completed suicides remained unchanged. There were no changes in the demographic factors, diagnoses, and occupational status of suicide attempts, but there was an increase in the percentage of individuals living alone who attempted suicide. The use of intoxications as a method for suicide attempts increased, and more people attempted suicide in their own homes.
EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE
(2023)
Article
Medicine, Legal
Constantin Lux, Mattias Kettner, Jan M. Federspiel, Frank Ramsthaler, Marcel A. Verhoff
Summary: This is a report on the autopsy analysis of a woman who was shot. She sustained three wounds, including a lethal penetrating head wound, a graze wound across the forehead, and a tangential penetrating wound in the thoracoabdominal region. Based on 3D reconstructed CT data, the bullet trajectory would have passed through the abdominothoracic cavities, right lung, and liver. However, the actual trajectory showed a prominent curve without deflection by bone. Possible explanations for this unusual bullet track include the woman's body movement at the time of the bullet impact and the shallow angle of incidence that caused the bullet to deflect intracutaneously. Additionally, the resistance of the soft tissue may have caused the bullet to yaw.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Medicine, Legal
Constantin Lux, Alexander Krutzek, Tobias Reich, Stephan Welkerling, Jan M. Federspiel, Frank Ramsthaler, Hannes Gruber, Patrick Sauer, Natascha Kern, Marcel A. Verhoff, Mattias Kettner
Summary: This study examined the formation of round-shaped compounded glass fragments after gunshots through windshields in an experimental setting. Using different projectiles and analyzing the morphology, impact angles, and terminal ballistics of the fragments, it was found that the morphology of the compounded glass fragments can differentiate the type of projectiles used, which can aid in understanding the atypical morphology of gunshot wounds.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Medicine, Legal
F. Holz, M. A. Verhoff, K. Mueckenberger, F. Kantor, J. E. Schulte, V. Krause, J. Kozlowski, C. G. Birngruber, C. Wunder, R. Zehner, S. Fischer, W. Betz, S. C. Koelzer
Summary: Excavations at Kalmenhof revealed a collective burial site consisting of two male infants and one female juvenile. There was no evidence of age-based cemetery subdivisions or graves on adjacent territories. The identity of the individuals was determined through examinations. The skeletal remains were reinterred in 2020.
Article
Medicine, Legal
S. Plenzig, M. Kettner, A. Berger, S. Ciesek, M. A. Verhoff, H. F. Rabenau
Summary: In spring 2022, protective measures against COVID-19 were lifted in several countries. Through PCR and cell culture testing, the Institute of Legal Medicine in Frankfurt/M. found that out of 24 autopsy cases with flu-like symptoms, 10 tested positive for various respiratory viruses, including SARS-CoV-2 and other viruses. This highlights the relevance of respiratory viruses other than SARS-CoV-2 in postmortem settings.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Medicine, Legal
Sarah C. Koelzer, Lena M. Bunzel, Franziska Holz, Christoph G. Birngruber, Marcel A. Verhoff
Summary: This case involves a woman in her thirties who suffered an esophageal rupture during extreme sadomasochistic activities. Initially diagnosed with broken ribs and a pneumothorax from a fall, further examination revealed the esophageal rupture caused by swallowing an inflatable gag. The woman had visible injuries from sadomasochistic acts, but her consent to such extreme sexual practices could not be definitively proven. The man involved was convicted and sentenced to a long-term imprisonment for intentional bodily harm.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Verena Wilmes, Ivan M. Kur, Andreas Weigert, Marcel A. Verhoff, Elise Gradhand, Silke Kauferstein
Summary: This study examines the expression of iNOS and NT production in postmortem human myocardial infarction hearts. The results demonstrate that resident macrophages are the main source of iNOS expression in these hearts, and there is a correlation between iNOS and NT production, indicating the involvement of iNOS in oxidative stress.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
