Article
Fisheries
Wenwen Wang, Wei Wang, Wenjun Xu, Qin Liu, Yuanxing Zhang, Xiaohong Liu
Summary: An inactivated vaccine incorporating polyactin, CpGriched plasmid and aluminum adjuvant was developed, showing a relative percentage survival of up to 64% against Pseudomonas plecoglossicida. However, the vaccine combination did not exhibit the strongest activation of humoral immune response, but was more likely to induce a cellular immune response.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Huazhi Chen, Yameng Zhang, Guangming Shao, You Chen, Yibo Shen, Yinnan Mu, Xinhua Chen
Summary: In this study, the transcriptomes of large yellow croaker spleens at 12 h and 24 h after Pseudomonas plecoglossicida infection were constructed. A total of 518 novel miRNAs and 823 known miRNAs were identified. Differential expression of miRNAs and mRNAs were observed at both timepoints, with immune-related genes being enriched. Network analysis revealed 47 mRNA-miRNA pairs, and functional validation confirmed the involvement of one miRNA in regulating the immune response.
Article
Fisheries
Junwei Huang, Xiaolong Yin, Hetron Mweemba Munang'andu, Cheng Xu, Weiye Li, Jianjun Xie, Xiaojun Yan, Zhen Tao
Summary: This study demonstrates that the orally administered live attenuated vaccine (LAV) developed by our laboratory provides significant protective immunity in large yellow croaker (LYC). Despite a decrease in survival after exposure to gastric acid, the LAV cells were able to reach the hindgut for absorption and did not have a long-term adverse effect on the gut microbiota. The vaccine also activated both cell-mediated and humoral immune responses.
Article
Fisheries
Haoda Ye, Zhijin Xu, Zhen Tao, Weiye Li, Yanyan Li, Amin Yang, Wei Wang, Xiaolong Yin, Xiaojun Yan
Summary: A highly attenuated P. plecoglossicida mutant Delta tssD-1 was constructed and shown to be safe and effective as a vaccine candidate in cultured large yellow croaker (LYC), providing protective immunity in LYC against P. plecoglossicida, with a relative percentage survival of 86.3%. This study represents the first report of a defined live attenuated strain for VGD in LYC.
Article
Fisheries
Yiying Li, Yu Chi, Shanshan Li, Tingting Jia, Zhijuan Mao
Summary: This study successfully identified a safe and highly immunogenic LAV candidate to combat P. plecoglossicida infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Zhaoyang Yin, Qiangde Liu, Yongtao Liu, Shengnan Gao, Yuliang He, Chuanwei Yao, Wenxing Huang, Ye Gong, Kangsen Mai, Qinghui Ai
Summary: Marine fish larvae are vulnerable during the early life period, but early intervention using probiotics, specifically Clostridium butyricum (CB), can improve growth performance, intestinal development, immune response, and gut microbiota. The study showed that CB supplementation in larval diets led to increased final length, weight, specific growth rate, and immune enzyme activities, as well as altered gut microbiota composition. Overall, the results suggest that early life intervention with 0.10-0.20% CB can promote growth of large yellow croaker larvae by enhancing intestinal development, immune responses, and modulating gut microbiota.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Feng-Ling Shu, Li-Yun Jin, Hao Liu, Zhen Tao, Fei Yin, Jia-Song Xie, Su-Ming Zhou
Summary: In this study, the researchers found that the galU gene of P. plecoglossicida is essential for its survival and virulence in large yellow croaker.
JOURNAL OF FISH DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Yameng Zhang, Lixia Lu, Chengwei Li, Guangming Shao, Xinhua Chen
Summary: The study revealed that the mRNA profile in the spleen of large yellow croaker changed significantly upon Pseudomonas plecoglossicida infection, with upregulation of many immune-related genes and activation of pathways related to ATP synthesis. These findings suggest potential defensive strategies used by the large yellow croaker in response to Pseudomonas plecoglossicida infection.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Nutrition & Dietetics
Zhaoyang Yin, Ye Gong, Yongtao Liu, Yuliang He, Chuanwei Yao, Wenxing Huang, Kangsen Mai, Qinghui Ai
Summary: This study demonstrates that feeding larimichthys crocea larvae with fucoidan-enriched diets significantly improves their growth performance, promotes digestive tract maturation, and maintains a healthy gut microbiota.
Article
Fisheries
Liangyin He, Youshen Li, Jiale Kang, Jianxin Li, Hetron Mweemba Munang'andu, Cheng Xu, Pan Qin, Xinhua Chen
Summary: A live attenuated Pseudomonas plecoglossicida mutant, constructed by knocking out the OmpR and rpoS genes, showed no significant difference in growth kinetics compared to the wild-type strain. The mutant strain exhibited a 1.17 x 107-fold decrease in virulence in the large yellow croaker and provided a relative percent survival of 72.25% and 64.90% at 4 and 6 weeks post-initial vaccination, respectively. This mutant strain is a promising candidate for a live attenuated vaccine against P. plecoglossicida infection.
Article
Fisheries
Weilu Qi, Qiancheng Gao, Jing Tian, Bi Wu, Miaozhen Lin, Shanni Qi, Qingpi Yan, Lixing Huang
Summary: The key virulence gene L321_RS13075 in Pseudomonas plecoglossicida was identified in this study, and knocking down this gene resulted in significant reductions in colonization ability, intracellular survival, motility, and other factors. The transcriptome of infected Epinephelus coioides was significantly altered by the silence of L321_RS13075, particularly affecting genes related to immune responses and inorganic ion transport. This study suggests that L321_RS13075 is a virulence gene that plays a crucial role in immune responses and ion transport in the host E. coioides.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Food Science & Technology
Luyao Huang, Xiaodan Lu, Huadan Zhang, Baodong Zheng, Yi Zhang, Peng Liang
Summary: This study found that large yellow croaker roe phospholipids (LYCRPLs) can positively regulate the gut microbiota by increasing the relative abundance of beneficial bacteria and decreasing harmful bacteria. The intervention of LYCRPLs also has potential ability to regulate glycolipid metabolism pathways in normal rats. These findings suggest that LYCRPLs have health-promoting effects and can be used as a functional ingredient in the food industry.
Review
Oncology
Yuting Lu, Xiangliang Yuan, Miao Wang, Zhihao He, Hongzhong Li, Ji Wang, Qin Li
Summary: The gut microbiota play a crucial role in modulating antitumor immunity and enhancing the efficacy of cancer immunotherapies, particularly immune checkpoint inhibitors. Metabolites are a key mechanism through which the gut microbiota impact antitumor immune responses.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Review
Immunology
Xinxin Hou, Zongmei Zheng, Jiao Wei, Ling Zhao
Summary: There is a relationship between gut microbial dysbiosis and colorectal cancer (CRC) initiation and progression, as well as with the host immune system. Understanding this interaction can lead to the development of microbiota-based immunotherapy strategies for CRC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Zixu Liu, Biao Yuan, Lingmin Zhao, Lixing Huang, Yingxue Qin, Jiaonan Zhang, Jiaolin Zhang, Bing Hu, Qingpi Yan
Summary: This study revealed the role of the fliK gene in P. plecoglossicida pathogenicity and identified the main pathways involved in the immune response of E. coioides.
FRONTIERS IN MARINE SCIENCE
(2022)