Article
Multidisciplinary Sciences
Xing Fu, Yuan Pan, Yili Wang, Zhaoqing Pei, Binjie Xu, Jing Zhang, Jinsong Su
Summary: This article developed a universal method for identifying components in Tibetan medicine using ion trap mass spectrometry (IT-MS), which can be applied to unknown compounds in Tibetan medicine. The method includes standardized and programmed protocols for sample preparation, MS setting, LC prerun, method establishment, MS acquisition, multiple-stage MS operation, and manual data analysis. Two representative compounds in the Tibetan medicine Abelmoschus manihot seeds were successfully identified using multiple-stage fragmentation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Chemistry, Analytical
Xuefei Zhong, Shruti Nayak, Lili Guo, Shivkumar Raidas, Yunlong Zhao, Rachel Weiss, Matthew Andisik, Chinnasamy Elango, Giane Sumner, Susan C. Irvin, Michael A. Partridge, Hong Yan, Sook E. Yen, Haibo Qiu, Yuan Mao, Albert Torri, Ning Li
Summary: REGEN-COV, a cocktail of two human monoclonal antibodies, has shown promising results in reducing SARS-CoV-2 viral load in COVID-19 patients. A liquid chromatography-mass spectrometry method was developed for the determination of REGEN-COV concentrations in serum samples from patients participating in clinical trials. The concentrations measured by this method were comparable to those measured by a validated immunoassay.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
David C. Koomen, Mark B. Meads, Dario M. Magaletti, Joy D. Guingab-Cagmat, Paula S. Oliveira, Bin Fang, Min Liu, Eric A. Welsh, Laurel E. Meke, Zhijie Jiang, Oliver A. Hampton, Alexandre Tungesvik, Gabriel De Avila, Raghunandan Reddy Alugubelli, Taiga Nishihori, Ariosto S. Silva, Steven A. Eschrich, Timothy J. Garrett, John M. Koomen, Kenneth H. Shain
Summary: Multiple myeloma is an incurable hematological malignancy that affects thousands of people annually in the U.S. The study reveals that drug-resistant cells have higher levels of pentose phosphate pathway metabolites, with alterations in purine, pyrimidine, and glutathione metabolisms. Personalized strategies and inhibition of specific enzymes may improve melphalan efficacy.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Samuel Bernardo-Bermejo, Jingchuan Xue, Linh Hoang, Elizabeth Billings, Bill Webb, M. Willy Honders, Sanne Venneker, Bram Heijs, Maria Castro-Puyana, Maria Luisa Marina, Erik B. van den Akker, Marieke Griffioen, Gary Siuzdak, Martin Giera, Elena Sanchez-Lopez
Summary: Analytical techniques with high sensitivity and selectivity are crucial in clinical sample analysis. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is the standard method, but it is expensive. This study demonstrates that similar results can be achieved using a simpler single mass spectrometry detector combined with enhanced in-source fragmentation/annotation and correlated ion monitoring. The protocol provides step-by-step instructions for optimizing the conditions for this technique, making it broadly applicable and cost-effective for quantifying small molecules.
Article
Toxicology
Jochem Louisse, Patrick P. J. Mulder, Arjen Gerssen, Geert Stoopen, Deborah Rijkers, Milou G. M. van de Schans, Ad A. C. M. Peijnenburg
Summary: “The study utilized a bioassay-directed analysis approach to identify potentially toxic PAs not yet included in monitoring programs. The findings suggest that this method is effective in identifying candidate PAs with genotoxic activity.”
ARCHIVES OF TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Yu Kang, Pei Cao, Xiujv Liu, Yiran Jin, Xiuling Yang, Zhiqing Zhang
Summary: This study investigated the inhibitory effect of the combination of epirubicin and berberine on MCF-7 cell proliferation and explored the intracellular pharmacokinetics. The results showed that berberine enhanced the inhibitory effect of epirubicin on MCF-7 cell proliferation and significantly increased the intracellular concentration of epirubicin. These findings provide a basis for further research on the drugs and their effects.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Applied
Eshani Nandita, Nikita P. Bacalzo, Christopher L. Ranque, Matthew J. Amicucci, Ace Galermo, Carlito B. Lebrilla
Summary: A new method was proposed in this study to depolymerize polysaccharides into oligosaccharide markers for rapid identification of food polysaccharides using HPLC-QTOF MS. The method showed high repeatability and validation, as confirmed by experimental results.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemical Research Methods
Sujun Li, Jianhui Zhu, David M. Lubman, He Zhou, Haixu Tang
Summary: Liquid chromatography coupled with tandem mass spectrometry is commonly used in large-scale glycoproteomic studies. A novel concurrent approach for glycopeptide identification in multiple related data sets is presented using spectral clustering and spectral library searching. Evaluation on two large-scale data sets showed that this approach can identify 105%-224% more spectra as glycopeptides compared to using Byonic alone, enabling the discovery of potential biomarkers in hepatocellular carcinoma.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Bingkun Yang, Yahui Li, Wenyan Guo, Qingning Zhang, Liangyu Pan, Kunfeng Duan, Panpan Zhang, Leiming Ren, Wei Zhang, Qiao Wang, Dezhi Kong
Summary: This study investigated the active peptides in Cerebrolysin (CBL), a peptide-rich preparation made from porcine brain. Different extraction methods were compared, and the MCX method coupled with PEAKS software was found to be the most effective. Two anti-inflammatory peptides and one antioxidant peptide were detected in CBL, laying the foundation for further research on its active ingredients.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Dicheng Ma, Guoxian Wang, Jiamei Zhu, Wei Mu, Daolong Dou, Feng Liu
Summary: This study investigates the biological activity and mode of action of trans-2-hexenal (T2H) against Fusarium graminearum, the main agent of Fusarium head blight (FHB). It was found that T2H can inhibit F. graminearum growth by multiple mechanisms and can be used as a biofumigant to control FHB occurrence in the field.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Mi Zhang, Kouharu Otsuki, Reo Takahashi, Takashi Kikuchi, Di Zhou, Ning Li, Wei Li
Summary: Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is a powerful tool for the rapid identification of compounds within natural resources. This study used LC-MS/MS analysis to investigate the presence of structurally diverse daphnane diterpenoids in Wikstroemia indica, significantly contributing to our understanding of bioactive diterpenoids in plants within the Thymelaeaceae family.
Article
Chemistry, Medicinal
Pablo Estevez, David Castro, Jose Manuel Leao-Martins, Manoella Sibat, Angels Tudo, Robert Dickey, Jorge Diogene, Philipp Hess, Ana Gago-Martinez
Summary: This study focused on the identification of toxic compounds produced by a G. australes strain from the Mediterranean Sea, revealing the presence of a novel MTX analogue named MTX5 and the absence of CTX precursors, highlighting the complexity of identifying CTX precursors in these regions.
Article
Biology
Jinci Yan, Chengli Fang, Gaohua Yang, Jianxu Li, Yanqiang Liu, Lu Zhang, Pengjie Yang, Jingyuan Fang, Yang Gu, Yu Zhang, Weihong Jiang
Summary: BBR has been identified as directly targeting FtfL, a key enzyme in C1 metabolism, in Peptostreptococcus anaerobius, inhibiting its enzymatic activities and interfering with the pathogenic bacteria in the gut. This study sheds light on the molecular mechanisms underlying BBR's anti-CRC effects and provides a new approach for controlling CRC.
Article
Biochemical Research Methods
Gauri Pradhan, J. M. Sneha, Babasaheb P. Sonwane, B. Santhakumari, Alka Rao, Mahesh J. Kulkarni
Summary: Therapeutic monoclonal antibodies are large and complex molecules, and biosimilar mAbs must show high similarity to the reference product in critical quality attributes. Various methods, including peptide mapping, are used for quality assessment and comparative analysis of mAbs, allowing for identification of differences between innovator and biosimilar products.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Zhao Yin, Juhua Yang, Rong Ning, Yanjun Liu, Maoxiao Feng, Chunmin Gu, Jia Fei, Yumin Li
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2018)
Article
Medicine, Research & Experimental
Chunming Gu, Yanjun Liu, Zhao Yin, Juhua Yang, Guiping Huang, Xuejiao Zhu, Yumin Li, Jia Fei
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Article
Engineering, Environmental
Zhao Yin, Kaiyu Jiang, Lei Shi, Jia Fei, Jie Zheng, Shiyi Ou, Juanying Ou
JOURNAL OF HAZARDOUS MATERIALS
(2020)
Article
Biotechnology & Applied Microbiology
Juhua Yang, Donglin Cao, Yanrong Zhang, Ruiming Ou, Zhao Yin, Yanjun Liu, Guiping Huang, Chunming Gu, Shengting Chen, Jia Fei
CANCER GENE THERAPY
(2020)
Article
Oncology
Zhao Yin, Guiping Huang, Chunming Gu, Yanjun Liu, Juhua Yang, Jia Fei
CLINICAL CANCER RESEARCH
(2020)
Article
Medicine, Research & Experimental
Juhua Yang, Zhao Yin, Yumin Li, Yanjun Liu, Guiping Huang, Chunming Gu, Jia Fei
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Food Science & Technology
Zhao Yin, Hongyang Guo, Kaiyu Jiang, Juanying Ou, Mingfu Wang, Caihuan Huang, Fu Liu, Weibin Bai, Jie Zheng, Shiyi Ou
JOURNAL OF FUNCTIONAL FOODS
(2020)
Article
Biochemistry & Molecular Biology
Kaiyu Jiang, Zhao Yin, Ping Zhou, Hongyang Guo, Caihuang Huang, Guangwen Zhang, Wenzhong Hu, Shiyi Ou, Juanying Ou