Review
Biotechnology & Applied Microbiology
Zoe Kampff, Douwe van Sinderen, Jennifer Mahony
Summary: The Streptococcus genus consists of both commensal and pathogenic species, and their cell surfaces are covered with a complex range of cell wall polysaccharides that facilitate their interactions and environmental adaptation. This review provides a comprehensive overview of the genetic and compositional diversity of streptococcal cell wall polysaccharides, including rhamnose-glucose polysaccharides, exopolysaccharides, and teichoic acids. It also highlights the species-specific combinations of cell wall polysaccharides, particularly the presence of rhamnose-glucose polysaccharides in certain species and the presence of teichoic acids in other species.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Oncology
Feng Wan, Hui Wang, Manliu Wang, Jicheng Lv, Minghui Zhao, Hong Zhang
Summary: Mucosal immune regulation plays a crucial role in the immunopathogenesis of IgA nephropathy (IgAN). This study provides evidence for the connection between the intestinal barrier and elevated circulating IgA and IgA-IgG levels in IgAN.
JOURNAL OF PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Katherine Lavelle, Irina Sadovskaya, Evgeny Vinogradov, Philip Kelleher, Gabriele A. Lugli, Marco Ventura, Douwe van Sinderen, Jennifer Mahony
Summary: This study reveals that the rgp gene cluster in Streptococcus thermophilus encodes the biosynthetic machinery for a polysaccharide essential for phage adsorption to the host cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Chemistry, Applied
Adrianna Kaczmarska, Piotr M. Pieczywek, Justyna Cybulska, Artur Zdunek
Summary: Rhamnogalacturonan I (RG-I), a complex component of the pectin family, plays a significant role in cell wall and plant biomechanics and exhibits gelling ability in solution.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Nelson Dzidzorgbe Kwaku Akpabli-Tsigbe, Juliet Osabutey, Benjamin Kumah Mintah, Kwaku Tano-Debrah, Yongkun Ma
Summary: This study aimed to extract chlorogenic acid from heilong48 soybean using solid-state fermentation by Lactobacillus casei and Lactobacillus helviticus mixed culture. The best-optimized conditions for solid-state fermentation were determined as a liquid-solid ratio of 32:68 (0.47), incubation time of 48 h, pH of 5, and temperature of 50°C. These conditions resulted in satisfactory chlorogenic acid yield, fermentation efficiency, and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity.
Article
Biochemistry & Molecular Biology
Evgeny Vinogradov, Frank St. Michael, Chantelle Cairns, Andrew D. Cox
Summary: This study reports the structures of the lipopolysaccharide O-chain (OPS) of two strains of Fusobacterium nucleatum, SB-106CP and HM-992, both isolated from cancerous tissues. These strains are associated with colorectal cancer and pregnancy complications. The OPS structure of F. nucleatum 12230, a transtracheal isolate, is also compared, showing differences between the strains.
CARBOHYDRATE RESEARCH
(2022)
Review
Food Science & Technology
Hao-Ying-Ye Yao, Jun-Qiao Wang, Jun-Yi Yin, Shao-Ping Nie, Ming-Yong Xie
Summary: Nuclear magnetic resonance (NMR) is widely used for analyzing the molecular structure and conformation of polysaccharides. It can infer linkage and sequence of sugar residues, as well as be applied in various aspects such as conformation, quantitative analysis, and interaction analysis of polysaccharide mixtures.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Nutrition & Dietetics
Xuan Ju, Xi Wu, Yukun Chen, Shanshan Cui, Zixuan Cai, Liang Zhao, Yanling Hao, Feng Zhou, Fang Chen, Zhengquan Yu, Dong Yang
Summary: This study identified a novel mode of communication between gut bacteria and their host, mediated by the LevH1 protein displayed on the surface of the bacteria. This protein binds to colorectal cancer cells and inhibits their proliferation. The mechanism of this communication is different from bacterial small metabolites and relies on the specific structure of the LevH1 protein.
Article
Biochemistry & Molecular Biology
Yameng Sun, Yijing Tao, Zhimin Geng, Fenglei Zheng, Ying Wang, Yujia Wang, Songling Fu, Wei Wang, Chunhong Xie, Yiying Zhang, Fangqi Gong
Summary: Through GWAS and linkage disequilibrium analysis, previous studies have found a significant association between the CaN-NFAT signal pathway and susceptibility to KD. However, the molecular basis of the CaN/NFAT pathway in KD remains poorly understood. In this study, we investigate the role of the Ca2+/CaN/NFAT signaling pathway in macrophages in vitro and in vivo on coronary artery lesions induced by LCWE.
Article
Biochemistry & Molecular Biology
Evelina L. Zdorovenko, Andrey V. Gannesen, Andrey S. Dmitrenok, Marina V. Zhurina, Sergey V. Mart'yanov, Alexander S. Shashkov
Summary: Two types of glycopolymers were isolated from the cell wall of Micrococcus luteus C01 using stepwise extraction with cold and hot 10% aq CCl3CO2H. The structures of these glycopolymers were established through compositional analysis and 1D and 2D NMR spectroscopy, with L-Glu representing glutamic acid.
CARBOHYDRATE RESEARCH
(2021)
Article
Immunology
Yuanhui He, Risu Na, Xiaoxi Niu, Bingbing Xiao, Huixia Yang
Summary: This study investigated the impact of Lactobacillus rhamnosus and Lactobacillus casei on Gardnerella species biofilm formation, finding that adding L. rhamnosus at different stages effectively suppressed biofilm formation. Additionally, the quantity of biofilm was found to be highest at a pH of 5.5 and after 36 hours.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pediatrics
Li-En Hsieh, Adriana H. Tremoulet, Jane C. Burns, Magali Noval Rivas, Moshe Arditi, Alessandra Franco
Summary: Lactobacillus casei cell wall extract (LCWE) induces in mice a vasculitis similar to Kawasaki disease (KD), and LCWE-specific T cells may be associated with human vascular inflammation, potentially involving regulation of T cells and central memory T cells in the immune response.
FRONTIERS IN PEDIATRICS
(2021)
Article
Food Science & Technology
Liliana Luca, Mircea Oroian
Summary: This study examined the testing of three prebiotics and their effects on the properties and survival rates of microcapsules under different conditions. The extrusion encapsulation technique was found to be effective in producing microcapsules that meet the requirements of the food industry. Using prebiotic encapsulation matrix can increase cell viability and protection, indicating its potential use in probiotic microcapsule production for the food industry.
Article
Agriculture, Multidisciplinary
Shu-Rui Zhong, Meng-Fan Li, Zhi-Hua Zhang, Min-Hua Zong, Xiao-Ling Wu, Wen-Yong Lou
Summary: The Maillard reaction products (MRPs) prepared by conjugating soy protein isolate (SPI) with oligosaccharides showed enhanced antioxidative activities, promoted the growth of Lactobacillus casei, and improved the survival rate under adverse conditions. These MRP-based SPI-oligosaccharide conjugates have great potential for microencapsulation of probiotics.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
S. Copin, J. Mougin, V. Raguenet, A. Robert-Pillot, G. Midelet, T. Grard, M. Bonnin-Jusserand
Summary: Vibrio sp., commonly found in aquatic ecosystems, play important roles in human health and aquaculture. This study suggests that PMA-qPCR methods may be more effective in detecting the viable state of Vibrio cells.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Fisheries
Roxane Roquigny, Julia Mougin, Cedric Le Bris, Maryse Bonnin-Jusserand, Perine Doyen, Thierry Grard
Summary: This study conducted in a seabass farm in northern France revealed that bacterial communities in biofilms and tanks were more diverse compared to water inlets. The majority of phylum diversity was composed of Proteobacteria and Bacteroidetes, with Vibrionaceae family isolates predominantly found in tank water samples. The persistence of Vibrionaceae in biofilms suggests their potential role as a reservoir in case of dysbiosis in aquaculture environments.
Article
Multidisciplinary Sciences
Ibrahima Drame, Christine Lafforgue, Cecile Formosa-Dague, Marie-Pierre Chapot-Chartier, Jean-Christophe Piard, Mickael Castelain, Etienne Dague
Summary: Lactic acid bacteria, especially Lactococcus lactis, are widely used in the food industry for the control and protection of fermented food manufacturing processes. Recent studies have shown that certain strains of lactic acid bacteria form complex biofilms with heterogeneous and aerial structures. The presence of pili in these strains affects the biomechanical properties of the biofilms, with non-piliated strains being smoother and stiffer compared to piliated strains. Additionally, the role of pili in biofilm cohesiveness was demonstrated through the adhesion of bacteria to the biofilm surface.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Julia Mougin, Roxane Roquigny, Christophe Flahaut, Maryse Bonnin-Jusserand, Thierry Grard, Cedric Le Bris
Summary: The study highlights the threat of vibriosis in seabass aquaculture, mainly caused by Vibrio harveyi. Monitoring the presence of V. harveyi in water and biofilms over time is crucial for understanding the dynamics of the pathogen. The constant presence of V. harveyi in rearing tanks and water inlets suggests an increased risk of vibriosis outbreaks, especially with rising water temperatures.
Article
Microbiology
Transito Garcia-Garcia, Sandrine Poncet, Elodie Cuenot, Thibaut Douche, Quentin Giai Gianetto, Johann Peltier, Pascal Courtin, Marie-Pierre Chapot-Chartier, Mariette Matondo, Bruno Dupuy, Thomas Candela, Isabelle Martin-Verstraete
Summary: The STK signaling pathway regulates PGH homeostasis to precisely control PG hydrolysis during cell division. The study on PG hydrolase CwlA in C. difficile reveals its important role in cell division and its regulation by PrkC, highlighting a novel regulatory mechanism by which STK controls protein export.
Editorial Material
Microbiology
Marie-Pierre Chapot-Chartier, Nienke Buddelmeijer
RESEARCH IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Meline Soret, Tiffanie Bouchendhomme, Jerome Cleach, Nathalie Jouy, Claire Crola Da Silva, Anne Devin, Thierry Grard, Philippe Lencel
Summary: Mitochondria can serve as an excellent marker for confirming seabass freshness, as the membrane potential gradually decreases over time and becomes difficult to repolarize through the addition of substrates.
Article
Biochemistry & Molecular Biology
Marta Toth, Szabolcs Muzsai, Krzysztof Regulski, Timea Szendi-Szatmari, Zsolt Czimmerer, Eva Rajnavolgyi, Marie-Pierre Chapot-Chartier, Attila Bacsi
Summary: The human gut symbiont Lacticaseibacillus (L.) casei has a wide range of immunomodulatory effects on the human host. This study demonstrates that human monocyte-derived dendritic cells (moDCs) can engulf L. casei BL23, and the intact bacterial cell wall and morphology play a key role in this process. The absence of the bacterial cell-wall-degrading enzyme Lc-p75 leads to defective internalization and impaired cytokine secretion by moDCs, resulting in reduced T-cell activation capacity. Formation of short bacterial chains helps to exert the potent immunomodulatory properties of L. casei BL23.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nikos Nikolopoulos, Renata C. Matos, Pascal Courtin, Isabel Ayala, Houssam Akherraz, Jean-Pierre Simorre, Marie-Pierre Chapot-Chartier, Francois Leulier, Stephanie Ravaud, Christophe Grangeasse
Summary: In this study, the cytoplasmic steps of the TA d-alanylation pathway in Lactiplantibacillus plantarum were investigated using structural biology and in vivo approaches. The protein interactions involved in this pathway were characterized, with DltC1 identified as a central hub for the cytoplasmic steps. The importance of AcpS, DltB, DltC1, and DltA in promoting Drosophila juvenile growth under chronic undernutrition was also established.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Tiffanie Bouchendhomme, Meline Soret, Anne Devin, Philippe Pasdois, Thierry Grard, Philippe Lencel
Summary: By investigating the properties of mitochondria, we were able to differentiate between fresh and frozen-thawed fish fillets based on the permeability of their membranes.
Review
Biochemistry & Molecular Biology
Hugo Guerin, Saulius Kulakauskas, Marie-Pierre Chapot-Chartier
Summary: Rha-CWPSs are crucial cell wall components of many important bacteria, playing a critical role in bacterial growth, division, and interactions with phages or hosts. This review summarizes the current knowledge on the structure, biosynthesis, and contribution of Rha-CWPSs to cell wall architecture and biogenesis, as well as their importance in cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Fisheries
Julia Mougin, Victor Lobanov, Morgane Danion, Roxane Roquigny, Lionel Goardon, Thierry Grard, Thierry Morin, Laurent Labbe, Alyssa Joyce
Summary: Misuse and overuse of antibiotics in aquaculture leads to bacterial resistance. Using immunostimulants in fish diets, specifically fungal and herbal compounds, is a sustainable alternative. This study evaluates the effects of fungal and herbal additives on rainbow trout, showing positive immune and gut microbiota responses without adverse effects on growth and survival. The findings contribute to the understanding and potential commercial use of immunostimulant compounds as feed supplements.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Food Science & Technology
Tiffanie Bouchendhomme, Meline Soret, Thierry Grard, Philippe Lencel
Summary: The permeability of red muscle fibres of Gilthead sea bream to NADH can be used to differentiate between fresh and frozen-thawed fish fillets.
Article
Biology
Nikos Nikolopoulos, Renata C. Matos, Stephanie Ravaud, Pascal Courtin, Houssam Akherraz, Simon Palussiere, Virginie Gueguen-Chaignon, Marie Salomon-Mallet, Alain Guillot, Yann Guerardel, Marie-Pierre Chapot-Chartier, Christophe Grangeasse, Francois Leulier
Summary: This study investigates the role of an uncharacterized protein (DltE) encoded by the dlt operon in Lactiplantibacillus plantarum. It is found that DltE acts as a D-Ala carboxyesterase, removing D-Ala from lipoteichoic acids (LTAs). The D-Ala-LTAs are shown to be important cues supporting intestinal peptidase expression and juvenile growth in Drosophila melanogaster.
Article
Food Science & Technology
Simge Duman, Perine Doyen, Pauline Merveillie, Nicolina Andersson, Romane Bayeuil, Thierry Grard, Alexandre Dehaut, Guillaume Duflos
Summary: This study aims to optimize methods for evaluating the presence of microplastics in the edible portions of seafood products available in supermarkets. The results showed the presence of microplastics in seven different seafood products, and the identification of the types of microplastics present.
