Article
Agriculture, Multidisciplinary
Xingtao Zhou, Duoduo Zhang, Wucheng Qi, Tao Hong, Tao Xiong, Tingqin Wu, Fang Geng, Mingyong Xie, Shaoping Nie
Summary: EPS116 promotes intestinal epithelial regeneration by increasing the number of intestinal stem cells and modulating the gut microbiota profile, thereby maintaining intestinal barrier and homeostasis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Yunyun Jiang, Min Zhang, Yang Zhang, Justyna Zulewska, Zhennai Yang
Summary: This study investigated the impact of Ca2+ on EPS biosynthesis in the probiotic Lactobacillus plantarum K25. Results showed that Ca2+ increased EPS yield, changed monosaccharide composition, upregulated proteins related to EPS synthesis, and influenced small molecule metabolites related to EPS biosynthesis pathways. The activation of specific signaling pathways by Ca2+ provided insights into the regulation of EPS synthesis genes in L. plantarum K25.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Shilei Huang, Qiuhan Yu, Luyi Xie, Ling Ran, Kai Wang, Yang Yang, Lu Gan, Zhenhui Song
Summary: The study showed that Lactobacillus plantarum metabolites and exopolysaccharides have inhibitory effects on Porcine Epidemic Diarrhea Virus replication, with exopolysaccharides exhibiting strong antiviral properties. Exopolysaccharides can prevent virus adsorption and induce early apoptosis of infected cells.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Kun-tai Li, Shuai-ying Peng, Bao Zhang, Wei-fu Peng, Shui-jing Yu, Xin Cheng
Summary: Exopolysaccharides (EPSs) can alleviate the toxic effect of cadmium (Cd) on rice and reduce Cd accumulation in grains. EPSs can facilitate the accumulation of Cd in rice roots and reduce Cd transportation from roots to leaves. Additionally, EPSs can affect the subcellular distribution and chemical forms of Cd in different parts of rice seedlings.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
M. P. Soumya, Reeba Parameswaran, K. Madhavan Nampoothiri
Summary: Glycosyltransferases synthesize exopolysaccharides (EPS) by modifying the properties of the polymers, such as glycosidic linkage, branching, length, mass, and conformation. A study on Lactobacillus plantarum BR2 (Accession No: MN176402) identified twelve glycosyltransferase genes, including BR2gtf (1116 bp), which was cloned into the pNZ8148 vector as an EPS biosynthetic glycosyltransferase. The over-expression of gtf gene in L. plantarum BR2 using a nisin-controlled expression system resulted in a 54.4% increase in EPS production, reaching a maximum of 23.2 ± 0.5 g/L in a 5 L bioreactor study after 72 h of fermentation. This study demonstrates a potential molecular strategy to enhance exopolysaccharide production in lactic acid bacteria.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
M. P. Soumya, K. Madhavan Nampoothiri
Summary: This study focuses on the production and chemical modifications of EPS produced by Lactobacillus plantarum BR2, and the evaluation of their biological properties. The results show that the modified derivatives have better antioxidant properties compared to the unmodified form. Among the derivatives, acetylated EPS (a-EPS) exhibits the highest DPPH radical scavenging and total antioxidant activity, while sulphated EPS (s-EPS) shows the highest reducing power potential and hydroxyl radical scavenging activities. The improved antioxidant properties open up better avenues for EPS application in the food and pharma sectors.
Article
Chemistry, Applied
Mengzhou Zhou, Xin Zheng, Hanjian Zhu, Leibing Li, Lin Zhang, Menglin Liu, Zeping Liu, Mingye Peng, Chao Wang, Qin Li, Dongsheng Li
Summary: The study demonstrated that selenium-enriched Lactobacillus plantarum R can enhance the antioxidant capacity of fermented pickles and inhibit the formation of AGEs, while the enrichment of organic/inorganic selenium can influence the composition of microbial communities.
Article
Chemistry, Applied
Xueliang Zhang, Gisele LaPointe, Yang Liu, Xiaomeng Wang, Luyao Xiao, Xiaogan Zhao, Wei Li
Summary: In this study, the impact of Lactiplantibacillus plantarum with ropy and non-ropy phenotypes on gel structure and protein conformation of fermented milk was evaluated. Ropy L. plantarum strains enhanced the viscosity and water holding capacity of fermented milk by secreting EPS with high molecular weight and intrinsic viscosity, forming a dense gel structure. Non-ropy L. plantarum strains resulted in high hardness and low water holding capacity of fermented milk due to high surface hydrophobicity and free sulfhydryl content. Raman spectroscopy and circular dichroism analysis revealed that the difference among gels was attributed to the high levels of alpha-helix and random coil protein structures. This study provides insights into the structural variability of fermented milk gels caused by ropy or non-ropy lactic acid bacteria.
Article
Environmental Sciences
Xinchi Shang, Longwu Geng, Zhigang Zhao, Liang Luo, Xiaodan Shi, Qing Zhang, Rujun Du, Yanfeng Cong, Wei Xu
Summary: This study used transcriptome analysis to investigate the possible mechanism of brain injury after cadmium exposure and the mitigation of selenium-enriched probiotics. The results revealed differentially expressed genes and enrichment of different signaling pathways. The findings provide a theoretical basis for further research on genes related to cadmium poisoning and the amelioration of selenium-enriched probiotics on cadmium poisoning.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Microbiology
Arum Darmastuti, Pratama N. Hasan, Rachma Wikandari, Tyas Utami, Endang S. Rahayu, Dian Anggraini Suroto
Summary: The adhesion properties of Lactobacillus plantarum Dad-13 and Mut-7 to the intestinal mucosa of Sprague Dawley rats were studied, showing their ability to adhere to the surface of the rat intestine. Genomic analysis revealed genes related to adhesion properties in both strains, suggesting their potential role in increasing probiotics' adherence to the intestinal mucosa.
Article
Food Science & Technology
Sendeku Takele Alemneh, Shimelis Admassu Emire, Bernd Hitzmann
Summary: This study evaluated the suitability of a teff-based substrate for developing probiotic functional beverages, finding that both Lactobacillus rhamnosus GG (LGG) and Lactobacillus plantarum A6 (LA6) could grow and produce desired products in the substrate. The combination of the two evaluated lactobacilli strains reduced fermentation time, indicating the potential for producing functional probiotic beverages using teff flour as a substrate without any supplements.
Article
Food Science & Technology
Yongfang Tang, Wang Chen, Hong Zhu, Cuiping Yi, Jieyao Yuan, Yanlan Liu, Zuyin Li, Hao Cheng
Summary: This study investigated the changes in digestibility and starch structure of indica rice during fermentation by Lactobacillus plantarum. The results showed that the glycemic index of the rice decreased, and the content of slowly digestible starch and resistant starch increased after fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Emilija Brdaric, Dusanka Popovic, Svetlana Sokovic Bajic, Dina Tucovic, Jelena Mutic, Maja Cakic-Milosevic, Sladana Durdic, Maja Tolinacki, Aleksandra Popov Aleksandrov, Natasa Golic, Ivana Mirkov, Milica Zivkovic
Summary: Through experiments on rats, it was found that food supplemented with EPS-AN8 can reduce the levels of cadmium in the kidneys, liver, and blood, while enriching it in the feces. In addition, EPS-AN8 can reverse the effects of cadmium on oxidative stress, inflammation, and gut microbiota. Therefore, EPS-AN8 may be extremely significant in combating this toxic environmental pollutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xinchi Shang, Wei Xu, Zhigang Zhao, Liang Luo, Qing Zhang, Muyang Li, Qingsong Sun, Longwu Geng
Summary: This study evaluated the protective effect of Se-enriched Lactobacillus plantarum on Luciobarbus capito exposed to waterborne Cd. The results showed that the dietary supplementation of Se-enriched L. plantarum can reduce Cd accumulation in tissues, enhance antioxidant enzyme activity, and reverse changes in intestinal microbial composition. Se-enriched L. plantarum effectively protects Luciobarbus capito against Cd toxicity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)