Article
Reproductive Biology
Yao Ma, Xu-dong Jiang, Da-wei Zhang, Xiang-dong Zi
Summary: In this study, the yak TGIF1 gene was cloned and its functions in yak granulosa cells (GCs) were investigated. The results showed that TGIF1 regulates the proliferation, apoptosis, cell cycle progression, and steroidogenesis in yak GCs.
Article
Cell Biology
Hanxiao Wu, Peihao Sun, Ce Lv, Xinzhe Zhao, Mingxiao Liu, Qunli Zhou, Jiaomei Tang, Liguo Yang, Aixin Liang
Summary: In this study, the expression and function of the IL-11 signaling receptor complex in bovine granulosa cells were investigated. Knockdown of IL-11R alpha was found to inhibit granulosa cell proliferation and steroidogenesis. Furthermore, the JAK1-STAT3, PKA-CREB, p38MAPK, and ERK1/2 signaling pathways were identified as mediators of IL-11/IL-11R alpha effects on granulosa cell function.
Article
Oncology
Xue Jiao, Xiruo Zhang, Nianyu Li, Dunfang Zhang, Shidou Zhao, Yujie Dang, Peter Zanvit, Wenwen Jin, Zi-Jiang Chen, Wanjun Chen, Yingying Qin
Summary: This study revealed an augmented T helper 1 (T(H)1) response and regulatory T (T-reg) cell deficiency in patients with premature ovarian insufficiency (POI), which was strongly correlated with disease severity. In mouse models, increased infiltration of T(H)1 cells in the ovary led to follicle atresia and ovarian insufficiency, while T-reg cells were able to prevent and reverse these effects. Furthermore, interferon (IFN) -gamma and tumor necrosis factor (TNF) -alpha were found to cooperate in promoting granulosa cell apoptosis and suppressing steroidogenesis in the context of POI.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Reproductive Biology
Patrycja Kurowska, Monika Dawid, Julia Oprocha, Natalia Respekta, Loise Serra, Anthony Estienne, Piotr Pawlicki, Malgorzata Kotula-Balak, Fabrice Guerif, Joelle Dupont, Agnieszka Rak
Summary: This study investigates the expression and regulatory function of the novel neuropeptide Spexin (SPX) in the human ovary, and its potential role in polycystic ovarian syndrome (PCOS) pathogenesis. The results show that SPX levels are decreased in obese and PCOS women, and SPX inhibits granulosa cell (GC) proliferation and steroidogenesis. GALR2/3 and kinases MAP3/1, AKT, and STAT3 are involved in mediating these effects.
BIOLOGY OF REPRODUCTION
(2023)
Article
Endocrinology & Metabolism
Marah Armouti, Miriam Rodriguez-Esquivel, Carlos Stocco
Summary: This study investigates the molecular mechanisms of Sik in inhibiting FSH actions in primary rat granulosa cells (GCs), and finds that SIK inhibition enhances the activation of the FSH signaling pathway. It also suggests that SIKs regulate the FSH pathway by affecting factors between cAMP/PKA and CREB.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Pharmacology & Pharmacy
Farima Rahimi Mansour, Mohammad Nabiuni, Elaheh Amini
Summary: Aluminum, a widely used metal, can affect the ovaries and granulosa cells by up-regulating steroidogenic and proliferative genes. Additionally, aluminum may induce issues such as apoptosis and autophagy.
Article
Veterinary Sciences
Borhan Shokrollahi, Hai-Ying Zheng, Xiao-Ya Ma, Jiang-Hua Shang
Summary: This study revealed that Apelin (APLN) is a pleiotropic peptide with various effects on the female reproductive system. APLN-13 enhanced the effect of IGF1 on progesterone production, but had minimal effect on FSH-induced progesterone secretion. Moreover, both APLN-13 and APLN-17 improved granulosa cell proliferation and antioxidant capacity, but their effects on Bax expression differed.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xuan Sun, Cheng Zeng, Feng Wang, Zhen Zhang, Fan Yang, Zhi-Peng Liu, Kang Li, Guo-Min Zhang
Summary: In this study, it was found that Neuromedin S (NMS) enhances steroidogenesis in goat ovarian granulosa cells (GCs) by promoting mitochondrial fusion and protecting mitochondrial function. NMS treatment increased estrogen production and up-regulated the expression of genes involved in steroid synthesis, while these effects were blocked by knockdown of NMUR2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jung-Chien Cheng, Xiaoyu Han, Qingxue Meng, Yanjie Guo, Boqun Liu, Tinglin Song, Yuanyuan Jia, Lanlan Fang, Ying-Pu Sun
Summary: This study reveals that HB-EGF can enhance the luteal function in humans by inducing StAR expression and progesterone production through the activation of the ERK1/2 signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Reproductive Biology
Dan Zhao, Yiling Fan, Xianrong Xiong, Shi Yin, Wei Fu, Yan Ma, Yongqi Yue, Zhidong Zhao, Jian Li, Yan Xiong
Summary: This study investigated the expression pattern of the TRIB1 gene in yak follicles and its effect on the steroidogenesis of granulosa cells. The results showed that TRIB1 protein was most abundant in granulosa cells and overexpression of TRIB1 significantly reduced the progesterone secretion of granulosa cells, while not affecting estradiol secretion. Furthermore, TRIB1 overexpression also led to a decrease in mitochondrial number and downregulation of mitochondrial biogenesis related genes.
Article
Endocrinology & Metabolism
Xiaotong Wang, Elaine Lee, Barbara F. Hales, Bernard Robaire
Summary: Organophosphate esters (OPEs) are widely used as flame retardants and plasticizers and can be found everywhere. Exposure to these chemicals may disrupt female sex hormone balance and affect female fertility. In this study, the effects of OPEs on KGN ovarian granulosa cells were investigated. OPEs altered the steroidogenic ability of these cells by dysregulating the expression of genes involved in steroid and cholesterol biosynthesis. These effects may have negative impacts on female reproduction.
Article
Agriculture, Dairy & Animal Science
G. Basini, S. Bussolati, M. Iannarelli, L. Ragionieri, S. Grolli, R. Ramoni, A. Dodi, F. Gazza, F. Grasselli
Summary: Irisin is present in swine ovarian follicle and has a biphasic effect on progesterone production. The highest concentrations of irisin stimulated metabolic activity and inhibited cell proliferation.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Ruhi Kabakci, Kendra L. Clark, Michele R. Plewes, Corrine F. Monaco, John S. Davis
Summary: The study investigated the effects of PFOA on bovine granulosa cell function. Results showed that PFOA inhibited steroid hormone secretion and altered the expression of key enzymes required for steroidogenesis. PFOA also caused mitochondrial dysfunction and increased autophagy. These findings suggest that PFOA may negatively impact livestock reproductive health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Agriculture, Dairy & Animal Science
Xiaopeng An, Heran Cao, Shujuan Liu, Binyun Cao
Summary: TGIF1 plays a crucial role in steroidogenesis in goat granulosa cells, with higher expression leading to the suppression of key genes involved in hormone production, resulting in lower levels of E-2 and P-4.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Endocrinology & Metabolism
Hai-Yun Guan, He-Xia Xia, Xiu-Ying Chen, Lu Wang, Zhi-Jing Tang, Wei Zhang
Summary: The activation of TLR4 was found to suppress CYP19A1 expression and E2 secretion in human granulosa cells via NF-κB signaling, which has implications for follicular development and estrogen synthesis regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Bo Pan, Julang Li
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2018)
Article
Cell Biology
Xiao-Feng Sun, Ya-Peng Li, Bo Pan, Yu-Feng Wang, Julang Li, Wei Shen
Article
Biochemistry & Molecular Biology
Yinshan Bai, Bo Pan, Xiaoshu Zhan, Hailey Silver, Julang Li
Summary: The nuclear-enriched miR-195-5p regulates the expression of Foxo3 in porcine granulosa cells, potentially involving AGO2 recruitment and histone modification processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Renee Emily Hilker, Bo Pan, Xiaoshu Zhan, Julang Li
Summary: miR-21 promotes estradiol production through inhibiting WT1 expression in granulosa cells.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2022)