Article
Environmental Sciences
Ruhi Kabakci, Kendra L. Clark, Michele R. Plewes, Corrine F. Monaco, John S. Davis
Summary: The study investigated the effects of PFOA on bovine granulosa cell function. Results showed that PFOA inhibited steroid hormone secretion and altered the expression of key enzymes required for steroidogenesis. PFOA also caused mitochondrial dysfunction and increased autophagy. These findings suggest that PFOA may negatively impact livestock reproductive health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Reproductive Biology
Dan Zhao, Yiling Fan, Xianrong Xiong, Shi Yin, Wei Fu, Yan Ma, Yongqi Yue, Zhidong Zhao, Jian Li, Yan Xiong
Summary: This study investigated the expression pattern of the TRIB1 gene in yak follicles and its effect on the steroidogenesis of granulosa cells. The results showed that TRIB1 protein was most abundant in granulosa cells and overexpression of TRIB1 significantly reduced the progesterone secretion of granulosa cells, while not affecting estradiol secretion. Furthermore, TRIB1 overexpression also led to a decrease in mitochondrial number and downregulation of mitochondrial biogenesis related genes.
Article
Biochemistry & Molecular Biology
Yong Wang, Yunxia Guo, Chunhui Duan, Junjie Li, Shoukun Ji, Huihui Yan, Yueqin Liu, Yingjie Zhang
Summary: In this study, a new lncRNA (lncGSAR) was identified and its effects on proliferation and steroid hormone secretion in ovine granulosa cells (GCs) were investigated. The results showed that lncGSAR can activate the SCAP/SREBP signaling pathway by sponging miR-125b to regulate steroid hormone secretion in GCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Kassim Traore, Prajakta More, Akhil Adla, Godwin Dogbey, Vassilios Papadopoulos, Barry Zirkin
Summary: MEHP inhibits steroid formation in MA-10 cells at least in part by its effects on mitochondrial function, including reductions in STAR levels, mitochondrial membrane potential and respiration, as well as an increase in mitochondrial superoxide production.
Article
Endocrinology & Metabolism
Marah Armouti, Miriam Rodriguez-Esquivel, Carlos Stocco
Summary: This study investigates the molecular mechanisms of Sik in inhibiting FSH actions in primary rat granulosa cells (GCs), and finds that SIK inhibition enhances the activation of the FSH signaling pathway. It also suggests that SIKs regulate the FSH pathway by affecting factors between cAMP/PKA and CREB.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhiqi Lei, Ilyas Ali, Min Yang, Caixia Yang, Yifei Li, Lian Li
Summary: This study investigated the deleterious effects of NEFA on bovine granulosa cells and its underlying molecular mechanism using an in vitro model. The results showed that high levels of NEFA led to the accumulation of reactive oxygen species, increased apoptosis-related factors, and disrupted steroid hormone synthesis in BGCs. In addition, NEFA inhibited the PI3K/AKT pathway and increased FoxO1 expression. These findings help to clarify the molecular mechanisms underlying the negative effects of NEFA on BGCs.
Article
Cell Biology
Jung-Chien Cheng, Xiaoyu Han, Qingxue Meng, Yanjie Guo, Boqun Liu, Tinglin Song, Yuanyuan Jia, Lanlan Fang, Ying-Pu Sun
Summary: This study reveals that HB-EGF can enhance the luteal function in humans by inducing StAR expression and progesterone production through the activation of the ERK1/2 signaling pathway.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Xuan Sun, Yifei Wang, Qingyang Mai, Yixuan Fan, Qunhao Huang, Yingli Du, Hai Huang, El-Samahy Ma, Feng Wang, Guomin Zhang
Summary: The study reveals that Neuromedin S (NMS) and its receptors are mainly expressed in Leydig cells (LCs) of goat testes, with the highest expression detected at age three months. NMS addition significantly enhances testosterone secretion and cell proliferation in vitro cultured goat LCs by modulating mitochondrial morphology, function, and autophagy. These findings provide a novel view of the regulatory mechanisms involved in male sexual maturation.
Article
Endocrinology & Metabolism
Hai-Yun Guan, He-Xia Xia, Xiu-Ying Chen, Lu Wang, Zhi-Jing Tang, Wei Zhang
Summary: The activation of TLR4 was found to suppress CYP19A1 expression and E2 secretion in human granulosa cells via NF-κB signaling, which has implications for follicular development and estrogen synthesis regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Wei Wu, Mark Ziemann, Kevin Huynh, Gang She, Zheng-Da Pang, Yi Zhang, Thy Duong, Helen Kiriazis, Tian-Tian Pu, Ru-Yue Bai, Jing-Jing Li, Yu Zhang, Ming-Xia Chen, Junichi Sadoshima, Xiu-Ling Deng, Peter J. Meikle, Xiao-Jun Du
Summary: The study revealed that the Hippo signaling pathway mediates mitochondrial damage by repressing mitochondrial genes, which causally promotes the development of dilated cardiomyopathy (DCM). Mitochondria in the hearts of DCM mice showed overt structural abnormalities and dysfunction.
Article
Engineering, Environmental
Jing-Cai Liu, Chun-Hua Xing, Yi Xu, Zhen-Nan Pan, Hao-Lin Zhang, Yu Zhang, Shao-Chen Sun
Summary: Exposure to DEHP during lactation reduces follicle numbers in suckling mice, alters gene transcription levels leading to decreased estradiol and increased oxidative stress. DNA damage marker and ovarian apoptosis are increased in DEHP-exposed suckling mice, with inhibition of granulosa cell proliferation. These changes result in abnormal spindle formation and chromosome misalignment during oocyte maturation.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Reproductive Biology
Yao Ma, Xu-dong Jiang, Da-wei Zhang, Xiang-dong Zi
Summary: In this study, the yak TGIF1 gene was cloned and its functions in yak granulosa cells (GCs) were investigated. The results showed that TGIF1 regulates the proliferation, apoptosis, cell cycle progression, and steroidogenesis in yak GCs.
Article
Reproductive Biology
Yi Sun, Huiting Chen, Sichuan Chen, Xueming Xu, Wenchang Zhang, Yuchen Li
Summary: This study investigated the mechanism underlying apoptosis induced by 2,5-hexanedione (2,5-HD), the main metabolite of n-hexane, in rat ovarian granulosa cells (GCs). It was found that 2,5-HD treatment significantly increased the apoptosis rate in GCs. mRNA microarray analysis identified differentially expressed genes, with significant enrichment in multiple signaling pathways, including the Hippo pathway. The Hippo signaling pathway was found to be inhibited during 2,5-HD-induced apoptosis, and downregulation of the pro-proliferation gene Ctgf and upregulation of the pro-apoptosis gene Puma were important. Decreased Ctgf expression was associated with decreased binding of YAP1 to TEAD, while increased PUMA expression was not associated with YAP1 binding to P73.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Xiaopeng An, Heran Cao, Shujuan Liu, Binyun Cao
Summary: TGIF1 plays a crucial role in steroidogenesis in goat granulosa cells, with higher expression leading to the suppression of key genes involved in hormone production, resulting in lower levels of E-2 and P-4.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Environmental Sciences
Loise Serra, Anthony Estienne, Giovanni Caria, Christelle Rame, Claudy Jolivet, Claire Froger, Abel Henriot, Laurence Amalric, Fabrice Guerif, Pascal Froment, Joelle Dupont
Summary: Five triazoles were found in soil or water samples from the Centre-Val de Loire region of France, and their effects on steroidogenesis in ovarian cells were studied. These triazoles reduced the secretion of progesterone and estradiol at non-cytotoxic doses.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Reproductive Biology
Dongxu Li, Zifei Liu, Mingtian Deng, Liang Liu, Jiawei Lu, Feng Wang, Yongjie Wan
Summary: The study showed that METTL3 plays a crucial role in regulating goat embryonic development under oxidative stress conditions, leading to developmental arrest by decreasing the expression levels of m6A-related genes and critical transcription factors. This research provides a foundation for further investigation into the mechanism of oxidative stress during early embryonic development in goats.
Article
Reproductive Biology
Xiaoxiao Gao, Xiaodan Li, Zhibo Wang, Kang Li, Yaxu Liang, Xiaolei Yao, Guomin Zhang, Feng Wang
Summary: Supplementing L-arginine (L-Arg) promotes the growth of endometrial ductal gland invaginations (DGI) and alleviates oxidative stress in nutrient-restricted sheep. In vitro, L-Arg reduces reactive oxygen species (ROS) release, enhances antioxidant enzyme activity, improves cell viability, and alleviates cell cycle arrest and apoptosis caused by oxidative stress.
Article
Reproductive Biology
Yongjin Bao, Xiaodan Li, M. A. El-Samahy, Hua Yang, Zhibo Wang, Fan Yang, Xiaolei Yao, Feng Wang
Summary: Activin/inhibin is important for Hu sheep fecundity, involved in ovarian follicular development. INHBA participates in activin A and inhibin A synthesis. Positive correlation noted between INHBA level and activin A/inhibin A secretion. INHBA knockdown/overexpression downregulated INHA expression. RNA-Sequencing revealed 1,687 DEGs (Fold change >= 2, FDR < 0.01), including upregulated and downregulated genes in INHBA interference group. GO enrichment showed involvement in cell cycle regulation, protein serine/threonine kinase activity, and actin cytoskeleton reorganization. DEGs significantly enriched in 40 KEGG pathways, including P53, progesterone-mediated oocyte maturation, and PI3K-AKT signaling pathways. Positive correlation between INHBA level and PI3K/Akt/mTOR pathway-related genes/proteins. Study enhances understanding of INHBA roles in prolific sheep GCs and molecular effects of low INHBA expression on GCs, clarifying reproductive failures.
Article
Oncology
Ming-Yang Ge, Zhi-Peng Liu, Yu Pan, Jiao-Yang Wang, Xiang Wang, Hai-Su Dai, Shu-Yang Gao, Shi-Yun Zhong, Xiao-Yu Che, Jing-Hua Zuo, Yun-Hua Liu, Xing-Chao Liu, Hai-Ning Fan, Wei-Yue Chen, Zi-Ran Wang, Xian-Yu Yin, Jie Bai, Yan-Qi Zhang, Yan Jiang, Yi Gong, Zhi-Yu Chen
Summary: This study found that both NLR and PLR could be used to effectively predict long-term survival in patients with pCCA who underwent curative resection. High NLR and high PLR were associated with poor overall survival and recurrence-free survival in pCCA patients.
FRONTIERS IN ONCOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Long Zhang, Chuntian Lu, Cuixia Yi, Zhipeng Liu, Yuhua Zeng
Summary: This study utilized the China Family Panel Survey data to explore the relationship between the Internet and physical fitness. The study found a significant correlation between the Internet and physical fitness behavior, with Internet use being the cause for increased fitness frequency. The study also identified social capital and health risk perceptions as the functional mechanisms through which the Internet influences fitness behavior.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Kaiping Deng, Zhipeng Liu, Yalong Su, Zhen Zhang, Yixuan Fan, Yanli Zhang, Feng Wang
Summary: RUNX1T1 plays a crucial role in myogenesis by promoting cell proliferation, inhibiting myogenic differentiation and regulating alternative splicing events. Knockdown of RUNX1T1 leads to the impairment of myotube formation through the disruption of the calcium signaling pathway and altered expression levels of muscle-specific isoforms of ROCK2.
Article
Biochemistry & Molecular Biology
Wei Guo, Yingbo Yang, Xu Zhang, Jinyuan Chen, Shuang Wu, Jinli Yang, Kunjing Qu, Ling He, Meijun Liu, Hongyin Hu, Zhipeng Liu, Guangpeng Ren
Summary: This study examines the genomic divergences between two sister plant species, Medicago ruthenica and M. archiducis-nicolai, that occur in northern China and the northeast Qinghai-Tibet Plateau, respectively, with overlapping distributions in the border of the two regions. The results show that the two species have diverged from each other in the Quaternary but have been in continuous contact with gene flow occurring between them. Positive selection signatures associated with genes involved in adaptations to arid and high-altitude environments are also discovered. These findings provide insights into the interspecific divergence of these two sister species.
Article
Geosciences, Multidisciplinary
Jingjing Wang, Xiaorong Wei, Xiaoxu Jia, Mingbin Huang, Zhipeng Liu, Yufei Yao, Mingan Shao
Summary: In this study, a depth distribution function-based empirical approach was developed to predict deep soil organic carbon (SOC) at the regional scale. The approach was validated using data from four regions and applied to China's Loess Plateau. The negative exponential function was found to best describe the vertical distribution of SOC in various regions. The parameters of the negative exponential function were linearly correlated to surface SOC and the changing rates of SOC within the topsoil. The empirical equations for predicting the negative exponential parameters were established, and the validation results showed that this approach can accurately predict SOC concentration in soils down to 500 cm depth.
SCIENCE CHINA-EARTH SCIENCES
(2023)
Editorial Material
Surgery
Shi-Yun Zhong, Zhi-Peng Liu, Yi-Shi Yang, Ting Yu, Xiao-Jun Wang
INTERNATIONAL JOURNAL OF SURGERY
(2023)
Article
Cell Biology
Kaiping Deng, Zhipeng Liu, Xiaodan Li, Zhen Zhang, Yixuan Fan, Qunhao Huang, Yanli Zhang, Feng Wang
Summary: Recent evidence suggests that m(6)A methylation plays a crucial role in skeletal muscle development through its regulation of TGF beta 1. Knockdown of TGF beta 1 inhibits myoblast proliferation and induces apoptosis. The presence of m(6)A in the 5 'UTR of TGF beta 1 promotes its decay and inhibits its expression, leading to a blockage in the TGF beta 1/SMAD2 signaling pathway. Targeted demethylation of TGF beta 1 m(6)A enhances the activity of the SMAD2 signaling pathway and promotes myoblast proliferation.
Article
Biochemistry & Molecular Biology
Yu Cai, Hua Yang, Zhen Wan, Pei-Yong Chen, Zhi-Bo Wang, Jin-Jing Guo, Daxiang Wang, Feng Wang, Yanli Zhang
Summary: In this study, RNA sequencing was performed on sheep pituitary glands with different fecundity to identify a novel candidate lncRNA LOC105613571 targeting BDNF related to gonadotropin secretion. The study found that the expression of lncRNA LOC105613571 and BDNF could be significantly upregulated by GnRH stimulation in sheep pituitary cells, and knockdown of lncRNA LOC105613571 downregulated gonadotropin secretion via inactivation of the AKT, ERK, and mTOR pathway. This study provides new insights into the molecular mechanisms of pituitary functions in sheep.
Article
Biochemistry & Molecular Biology
Yu Cai, Peiyong Chen, Hui Xu, Shanglai Li, Bingru Zhao, Yixuan Fan, Feng Wang, Yanli Zhang
Summary: In this study, it was found that EZH2 gene knockdown in sheep pituitary cells led to decreased cell proliferation, increased apoptosis, and reduced FSH secretion. These effects were mediated through the modulation of the AKT/ERK signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Yifan Li, Shihong Zhong, Zihan Jin, Guofu Ye, Tianling Zhang, Zhipeng Liu, Zhenguo Liu, Zhaofeng Zeng, Qiong Li, Yuhao Wang, Yanda Zhao, Libo Tang, Huaihong Chen, Yongyin Li
Summary: This study aims to elucidate the role of Peyer's patches (PPs) in gut microbiota-mediated anti-HBV adaptive immunity. The results showed that depletion of gut microbiota impaired systemic adaptive immune responses and delayed HBV antigen clearance. Additionally, the depletion of PPs abolished gut microbiota-boosted intrahepatic HBV-specific T cell response.
Article
Biochemistry & Molecular Biology
Yingnan Yang, Yu Cai, Jinjing Guo, Keke Dai, Liang Liu, Zili Chen, Feng Wang, Mingtian Deng
Summary: KDM5B is crucial for early embryo development and its expression is highly regulated by maternal factors. In this study, we investigated the role of KDM5B in granulosa cells (GCs) and found that KDM5B is involved in cell cycle regulation and DNA damage prevention in GCs. Knockdown of KDM5B led to a decrease in cell proliferation and an increase in apoptosis, as well as a blockage of GCs at the S phase. Furthermore, knockdown of KDM5B resulted in increased reactive oxygen species levels, mitochondrial depolarization, and DNA double-strand breaks. These effects were partially rescued by up-regulation of MTF1. Overall, our findings suggest that KDM5B plays a critical role in promoting cell cycle progression and preventing DNA damage in GCs through the up-regulation of MTF1.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
