Article
Chemistry, Multidisciplinary
Xiao-Yu Wang, Chun-Jing Chen, Yao-Hui He, Lian-Shuai Ding, Yi-Fan Wu, Cheng-Ting Huang, Jun Wu, Rong Ding, Yu-Hua Xue, Zhi-Wei Lin, Peng-Xiang Xu, Yi-Le Wu, Wen Liu, Ji-Jun Li, Si-Ming Chen, Yu-Fen Zhao, Ji-Yang Dong, Qiang Zhou, Xiang Gao
Summary: Stable isotope chemical labeling methods have limitations in high sensitivity detection for quantitative proteomics. A novel isobaric stable isotope N-phosphorylation labeling strategy (iSIPL) was developed for precise quantitative proteome analysis with high sensitivity and accuracy. iSIPL strategy was successfully applied to analyze the dynamics of protein-protein interactions in the transactivation of HIV-1 provirus.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Xiaoling Su, Deying Chen, Liang Li, Lanjuan Li
Summary: The study demonstrates that perfusion can significantly alter the liver metabolome, recommending the use of non-perfused liver for comprehensive liver metabolomics analysis for high coverage.
ANALYTICA CHIMICA ACTA
(2021)
Article
Marine & Freshwater Biology
Eric Bollinger, Jochen P. Zubrod, Marco Konschak, Ralf Schulz, Mirco Bundschuh
Summary: Stable isotope analysis is an important technique in food web ecology, but its effectiveness can be unclear in complex systems. The use of heavy isotope tracers has been suggested as a way to enhance the utility of stable isotope analysis, but this study found that labeling could potentially affect isotopic fractionation and bias conclusions. While the physiological and activity levels of organisms in autotrophy-based and detritus-based aquatic food webs were not significantly affected by labeling, the study supported the isotopic redundancy hypothesis, which suggests that metabolic processes can be altered by discrete quantum mechanical states.
AQUATIC TOXICOLOGY
(2023)
Article
Chemistry, Applied
Huizhen Wu, Zhi Liu, Hu Zhang, Shuangnan Xie, Chao Tang, Wei Liu, Bin Li, Piao Chen, Zihong Zhang, Mingrong Qian
Summary: In this study, a method using GC-C-IRMS analysis and pre-column derivatization was developed to determine the 815N stable ratio of SEM in shrimp and crab. The results showed that the 815N values of SEM significantly decreased after the use of nitrofurazone. Furthermore, the analysis of SEM in crustaceans indicated a significant difference between SEMex and SEMend, which can be used for determining the source of SEM in vivo.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Soil Science
Zhiying Liang, Binbin Cao, Yapeng Jiao, Conghui Liu, Xiushuang Li, Xiangtian Meng, Jianglan Shi, Xiaohong Tian
Summary: Nitrogen fertilization can impact soil respiration by affecting the decomposition of soil organic matter and plant residues through microbial biomass. The conversion of mineral nitrogen to stable organic nitrogen (humus nitrogen) remains unclear, particularly in the presence of straw. Experiment results reveal a two-phase effect of nitrogen addition on CO2 emissions, indicating rapid utilization by microorganisms. Additionally, the study shows that stable organic nitrogen in humus can be converted by microbes, and the combination of nitrogen and straw promotes this conversion.
APPLIED SOIL ECOLOGY
(2022)
Article
Pharmacology & Pharmacy
Sabrina Wohlfart, Michael Kilian, Philip Storck, Thomas Gutsmann, Klaus Brandenburg, Walter Mier
Summary: A novel method for precise quantification of Pep19-2.5 in biological samples has been developed in this study, which is crucial for the treatment of sepsis. The method utilizes mass spectrometry and labeled amino acid residues to reliably quantify the peptide content.
Article
Chemistry, Analytical
Wenlong Sheng, Xue Zhang, Miaohui Yu, Meng Jin, Ning Li, Chen Sun, Lizhen Wang, Qing Xia, Xiaobin Li, Yun Zhang, Baocun Zhu, Kechun Liu
Summary: A novel fluorescent probe Mem-FA was developed to sensitively detect and distinguish internal and external sources of formaldehyde on cell membrane, showing potential for specific tracing of formaldehyde-induced diseases.
Article
Chemistry, Applied
Junfu Ji, Xinyu Liu, Xiaosong Hu, Fang Chen, Christoph Bueschl, Rainer Schuhmacher, Andrew L. Waterhouse, Lingjun Ma
Summary: This study successfully identified quinone reaction products in wine using a stable-isotope labelling approach combined with high-resolution mass spectrometry. Different nucleophiles were found to react in white wines and red wines.
Article
Chemistry, Analytical
Yanxin Li, Chong Ma, Jinmao You, Shijuan Zhang
Summary: The study prepared deuterated dansylhydrazine through a one-step simple reaction, applied it to stable isotope labeling of aldehydes, enhanced quantitation accuracy, and minimized matrix effect.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Yan-Qing Chen, Hong Shen, Ru-Jie Yang, Jian-Bo Wan
Summary: A sensitive method was developed to accurately determine 20 NAEs in biological samples by chemical isotope labeling strategy coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Derivatized NAEs provided stable and abundant specific product ions in MS/MS spectrum for quantitation, enhancing detection sensitivity by 6- to 170-fold compared to non-derivatized NAEs. The method was successfully applied to determine NAEs in serum samples and liver tissues, showing practicability in analyzing endogenous NAE in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Jie Zheng, Junjie Yang, Fanrong Zhao, Bo Peng, Yulan Wang, Mingliang Fang
Summary: In this study, a highly sensitive and high-throughput platform, CIL-ExPMRM, was developed for the analysis of isotope-labeled urinary exposure biomarkers. This platform overcomes key bottlenecks in exposome studies and outperforms previous methods due to its independence of MS/MS availability, accurate retention time prediction, collision energy optimization, and ultrasensitivity. Overall, CIL-ExPMRM has great potential to advance exposomic studies based on urinary biomarkers.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Cassio Lima, Howbeer Muhamadali, Royston Goodacre
Summary: We report the use of a novel technology, optical photothermal infrared (O-PTIR) spectroscopy, to simultaneously obtain infrared and Raman spectra from the same location of the sample for studying bacterial metabolism. The obtained infrared data from bulk populations and single cells were compared to conventional Fourier transform infrared (FTIR) spectroscopy to evaluate reproducibility. Raman spectra were analyzed to evaluate the isotopic incorporation. The study showed full incorporation of heavy isotopes by bacteria and successful discrimination between different labeled bacteria using Raman spectra. The limitations of O-PTIR technology to acquire Raman data from single bacterial cells and spectral artifacts induced by thermal damage were also discussed.
Article
Energy & Fuels
Qiong Wang, Hao Xu, Dazhen Tang, Pengfei Ren
Summary: This study investigates the composition and formation mechanisms of coalbed gas (CBG) in the Miquan and Fukang areas of the southern Junggar Basin. It is found that there are significant differences in the CBG composition between the two areas. The CBG in the Fukang area is mainly methane, while the CBG in the Miquan area has a high CO2 concentration. The production pathways of biogas also differ between the two areas, with the Fukang area mainly using the CO2 reduction pathway and the Miquan area mainly using the acetic acid/methyl fermentation pathway. This study clarifies the fate of CO2 in the Miquan area and highlights the influence of exogenous CO2 on microbial methanogenic pathways.
Review
Food Science & Technology
Shanshan Zhao, Haijin Liu, Mengjie Qie, Jiukai Zhang, Liqin Tan, Yan Zhao
Summary: Stable isotope abundance, as a natural fingerprint of food growing environment, has attracted great attention in tracing and adulterating analysis of animal products. In recent years, the combination of stable isotope ratio analysis and other methods has become a promising approach for ensuring the authenticity and traceability of animal products.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Engineering, Environmental
Yao Lu, Chao Wang, Xin-Yang Zhang, Zhi-Wei Wang, Zhi-Min Song, Ye Du, Qing Hu, Qian-Yuan Wu, Hong-Ying Hu
Summary: The study found that during ozonation in the presence of bromide and ammonia, the formation of nitrogenous byproducts may lead to increased toxicity. Using UPLC-HRMS and an isotope labeling strategy, four newly formed nitrogenous byproducts were detected. This highlights the importance of paying attention to nitrogenous byproducts during ozonation in the presence of bromide and ammonia.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Grace A. Chappell, Jennifer W. Israel, Jeremy M. Simon, Sebastian Pott, Alexias Safi, Karl Eklund, Kenneth G. Sexton, Wanda Bodnar, Jason D. Lieb, Gregory E. Crawford, Ivan Rusyn, Terrence S. Furey
ENVIRONMENTAL HEALTH PERSPECTIVES
(2017)
Article
Engineering, Environmental
Zhenyu Tian, Joaquim Vila, Hanyan Wang, Wanda Bodnar, Michael D. Aitken
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2017)
Article
Biochemical Research Methods
Zachary Robbins, Wanda Bodnar, Zhenfa Zhang, Avram Gold, Leena A. Nylander-French
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Jennifer W. Israel, Grace A. Chappell, Jeremy M. Simon, Sebastian Pott, Alexias Safi, Lauren Lewis, Paul Cotney, Hala S. Boulos, Wanda Bodnar, Jason D. Lieb, Gregory E. Crawford, Terrence S. Furey, Ivan Rusyn
Article
Engineering, Environmental
Zhenyu Tian, Joaquim Vila, Miao Yu, Wanda Bodnar, Michael D. Aitken
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2018)
Article
Chemistry, Analytical
Tianqu Cui, Zhexi Zeng, Erickson O. dos Santos, Zhenfa Zhang, Yuzhi Chen, Yue Zhang, Caitlin A. Rose, Sri H. Budisulistiorini, Leonard B. Collins, Wanda M. Bodnar, Rodrigo A. F. de Souza, Scot T. Martin, Cristine M. D. Machado, Barbara J. Turpin, Avram Gold, Andrew P. Ault, Jason D. Surratt
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
(2018)
Article
Toxicology
Lauren Lewis, Grace A. Chappell, Tetyana Kobets, Bridget E. O'Brian, Dewakar Sangaraju, Oksana Kosyk, Wanda Bodnar, Natalia Y. Tretyakova, Igor P. Pogribny, Ivan Rusyn
ARCHIVES OF TOXICOLOGY
(2019)
Article
Toxicology
Jiapeng Leng, Chih-Wei Liu, Hadley J. Hartwell, Rui Yu, Yongquan Lai, Wanda M. Bodnar, Kun Lu, James A. Swenberg
ARCHIVES OF TOXICOLOGY
(2019)
Article
Chemistry, Medicinal
Lauren Lewis, Barbara Borowa-Mazgaj, Aline de Conti, Grace A. Chappell, Yu-Syuan Luo, Wanda Bodnar, Kranti Konganti, Fred A. Wright, David W. Threadgill, Weihsueh A. Chiu, Igor P. Pogribny, Ivan Rusyn
CHEMICAL RESEARCH IN TOXICOLOGY
(2019)
Article
Environmental Sciences
Joaquim Vila, Zhenyu Tian, Hanyan Wang, Wanda Bodnar, Michael D. Aitken
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Environmental Sciences
Jiaqi Zhou, Karsten Baumann, Naomi Chang, Glenn Morrison, Wanda Bodnar, Zhenfa Zhang, Joanna M. Atkin, Jason D. Surratt, Barbara J. Turpin
Summary: This study investigated the emission of PFASs during floor stripping/waxing activities and their potential occupational exposures. The study found that concentrations of five PFASs in the air were significantly higher during floor stripping/waxing compared to before, and were one order of magnitude higher than typical indoor and outdoor concentrations. These results imply that floor stripping activities may lead to occupational PFAS exposures.
ATMOSPHERIC ENVIRONMENT
(2022)
Article
Environmental Sciences
Suryakant Niture, Sashi Gadi, Minghui Lin, Qi Qi, Samiksha S. Niture, John T. Moore, Wanda Bodnar, Reshan A. Fernando, Keith E. Levine, Deepak Kumar
Summary: This study investigated the effects of low concentrations of cadmium (Cd) on liver cancer cell proliferation, steatosis, and fibrogenic/oncogenic signaling. Cd exposure increased reactive oxygen species production, enhanced cell proliferation, and activated Notch and AKT/mTOR pathways in liver cancer cells. Cd also induced cell steatosis and fibrogenic signaling. These findings suggest that low concentrations of Cd modulate steatosis, fibrogenic, and oncogenic signaling in liver cancer cells.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Suzanne D. Baker, Jessica Y. Lee, Raymond P. White, Leonard Collins, Wanda Bodnar, Ceib Philips, Kimon Divans
Summary: This study compared the use of buffered one percent and unbuffered two percent lidocaine in children, finding that the buffered formulation resulted in a 63% decrease in mean blood lidocaine levels compared to unbuffered. However, there were no significant differences in pain upon injection, onset, and duration of anesthesia.
PEDIATRIC DENTISTRY
(2021)
Article
Chemistry, Analytical
J. Zhou, K. Baumann, R. N. Mead, S. A. Skrabal, R. J. Kieber, G. B. Avery, M. Shimizu, J. C. DeWitt, M. Sun, S. A. Vance, W. Bodnar, Z. Zhang, L. B. Collins, J. D. Surratt, B. J. Turpin
Summary: This study measured PFAS concentrations in ambient PM2.5 in North Carolina, finding higher levels of PFOA and PFOS and raising questions about their potential sources. It is the first study in the US to provide insights into ambient PFAS concentrations in PM2.5.
ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS
(2021)
Review
Environmental Sciences
Pengcheng Tu, Liang Chi, Wanda Bodnar, Zhenfa Zhang, Bei Gao, Xiaoming Bian, Jill Stewart, Rebecca Fry, Kun Lu