Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wen Zhang, Haofei Zhang
Summary: Iodide-adduct chemical ionization mass spectrometry (I-CIMS) is widely used in atmospheric chemistry to detect oxygenated volatile organic compounds (OVOCs) in real time. The study reports secondary ion chemistry from interactions between strong oxygen donors and acidic OVOCs in I-CIMS, which can lead to misinterpretations of gas-phase products' compositions and distributions. However, the varying degrees of signal change in response to secondary ion chemistry may provide clues to OVOCs' functionalities.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Houssni Lamkaddam, Josef Dommen, Ananth Ranjithkumar, Hamish Gordon, Gunther Wehrle, Jordan Krechmer, Francesca Majluf, Daniil Salionov, Julia Schmale, Sasa Bjelic, Kenneth S. Carslaw, Imad El Haddad, Urs Baltensperger
Summary: The study reveals that processing isoprene oxidation products in cloud droplets can produce a significant amount of secondary organic aerosol, which may have a substantial impact on the global aerosol burden.
Article
Biochemical Research Methods
Haibin Li, Chusheng Liu, Li Zhao, Dongsheng Xu, Tingting Zhang, Qiqin Wang, Deirdre Cabooter, Zhengjin Jiang
Summary: An unmatched or improperly matched sample solvent can distort the peak shape of analytes in chromatography. For retained compounds, pure ACN is the best sample solvent, while for compounds eluting close to the solvent peak, distortions occur with pure ACN. Water in the sample solvent favors electrostatic attraction for charged compounds, leading to increased retention time.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Fei Wang, Fan Yang, Jiawei Liu, Quan Bai
Summary: This study investigates the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism is studied in detail using a β-CD HILIC column. The study finds that a four-parameter equation derived from SDT-R accurately describes the U-shaped curves of solutes with RPLC/HILIC dual-retention mechanisms on the β-CD column, providing a theoretical guide for the development of HILIC and the enhancement of separation efficiency.
Article
Biochemical Research Methods
Xiaoyu Zhou, Hongyan Zhang, Li Wang, Ren'an Wu
Summary: This study developed a dual-functionalized metal-organic framework UIO-PBA&FDP for the efficient enrichment of glycopeptides in proteomics using the HILIC strategy. The UIO-PBA&FDP exhibited excellent hydrophilicity and successfully enriched 359 N-linked glycopeptides from human serum, achieving a high coverage of 104 glycoproteins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Xiaoya Zhang, Shuweng Hua, Quanshou Feng, Chuan-Fan Ding, Yongyao Wu, Yinghua Yan
Summary: Novel hydrophilic poly(N, N-methylenebisacrylamide/1,2-epoxy-5-hexene) coated magnetic nanospheres functionalized with 2-aminopurine (Fe3O4@poly(MBA/EH)@2AP) were successfully obtained using a simple and green method. The material showed high hydrophilicity and could capture glycopeptides and glycosylated exosomes within 1 min. It demonstrated great sensitivity, loading capability, selectivity, and repeatability, making it suitable for practical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Taslyne Imame Hassane Beck, Balthazar Toussaint, Estelle Surget, Christine Herrenknecht, Vincent Boudy, Emmanuel Jaccoulet
Summary: The HILIC-CAD method serves as an alternative for analyzing tromethamine, especially in drugs containing low concentrations of tromethamine in complex buffered formulations. By optimizing parameters, the method achieves a linear response, meets precision and accuracy requirements, and has been successfully applied to the analysis of sterile tromethamine injection solutions.
Article
Biochemical Research Methods
Ruben Pawellek, Ulrike Holzgrabe
Summary: The study aimed to compare the performance of IPC and HILIC when coupled to the CAD, finding that HILIC showed significantly higher CAD response and obtainable S/N compared to IPC. Additionally, IPC and HILIC methods were developed for the impurity profiling of BCAAs, with HILIC method showing slightly superior sensitivity and separation efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Engineering, Environmental
Ziying Lei, Yuzhi Chen, Yue Zhang, Madeline E. Cooke, Isabel R. Ledsky, N. Cazimir Armstrong, Nicole E. Olson, Zhenfa Zhang, Avram Gold, Jason D. Surratt, Andrew P. Ault
Summary: The study demonstrates that initial aerosol acidity plays a significant role in shaping the physicochemical properties of SOA formed from IEPDX, with higher acidity resulting in more organosulfate formation and a tendency for the organic phase to transition to a semi-solid or solid state. These findings can be used to improve model parameterizations of SOA formation and properties from IEPDX to better align predictions with atmospheric observations.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemical Research Methods
N. Yu Chikurova, A. O. Shemiakina, O. A. Shpigun, A. Chernobrovkina
Summary: A series of novel HILIC stationary phases containing both amides and hydroxyl groups were obtained on an aminated silica surface through multicomponent Ugi reaction. These stationary phases showed good separation efficiency and selectivity in chromatographic parameters and model mixtures.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Peizhen Li, Rongmao Lu, Jie Zhu, Qingqing Ke, Dingnan Wang, Yang Wang, Chen Zhang, Xinyue Lu, Miaolong Zhao, Zhang Wang, Lingling Pan, Meiling Zhang, Jun Ying, Shiyan Li
Summary: A sensitive and reliable ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry method was developed to determine paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) in marine shellfish. The method involved the use of a HILIC-Z column for chromatographic separation and a porous carbon solid-phase extraction for sample clean-up. The calibration curves for PSTs and TTX were linear, with good sensitivity and low limits of detection and quantitation. The method was successfully applied to determine PSTs and TTX in three batches of Nassarius, showing excellent accuracy.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Review
Environmental Sciences
Magda Claeys, Willy Maenhaut
Summary: This review covers research on secondary organic aerosol (SOA) formation from isoprene over the past two decades, highlighting the complex pathways and source apportionment. The importance of molecular characterization and mass spectrometric techniques in understanding the formation mechanisms is emphasized. Efforts to distinguish different sources of SOA, including isoprene SOA, using organic marker-based methods and positive matrix factorization are examined.
Article
Environmental Sciences
Sarah E. Orr, Leonard B. Collins, Dereje D. Jima, David B. Buchwalter
Summary: Ecologists have observed declines in the biodiversity of sensitive freshwater organisms in response to increasing salinization, but the physiological challenges faced by aquatic organisms, such as mayflies, are not well studied. This study used proteomics to identify specific proteins associated with ion transport in mayfly gills and investigate how their abundance changes in response to chronic salinity exposure. The research found differential protein expression in gills under different salinity conditions, and also explored non-transport related pathways in the gills. The study highlights the importance of understanding the impacts of salinization on non-model species.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Suryakant Niture, Sashi Gadi, Minghui Lin, Qi Qi, Samiksha S. Niture, John T. Moore, Wanda Bodnar, Reshan A. Fernando, Keith E. Levine, Deepak Kumar
Summary: This study investigated the effects of low concentrations of cadmium (Cd) on liver cancer cell proliferation, steatosis, and fibrogenic/oncogenic signaling. Cd exposure increased reactive oxygen species production, enhanced cell proliferation, and activated Notch and AKT/mTOR pathways in liver cancer cells. Cd also induced cell steatosis and fibrogenic signaling. These findings suggest that low concentrations of Cd modulate steatosis, fibrogenic, and oncogenic signaling in liver cancer cells.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Biochemical Research Methods
Hongxia Bai, Leonard B. B. Collins, Marcos Rogerio Andre, Edward B. B. Breitschwerdt, Taufika Islam Williams
Summary: A bottom-up proteomics workflow was developed to study a system containing multiple organisms, allowing a thorough understanding of their interactions. The workflow utilized label-free quantification proteomics with nanoLC-MS/MS. Detailed instructions were provided to promote the adoption of this workflow in other laboratories.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Materials Science, Biomaterials
Andreea Biehl, Ana M. Gracioso M. Martins, Zachary G. G. Davis, Daphne Sze, Leonard Collins, Camilo Mora-Navarro, Matthew B. B. Fisher, Donald O. O. Freytes
Summary: The present study is the first step towards developing standardized protocols for efficient decellularization of multiple tissues. Through an automated decellularization method, the researchers were able to produce cytocompatible extracellular matrix (ECM) hydrogels with consistent properties from various tissues. This study demonstrates the potential for fast, scalable, and reproducible derivation of ECM biomaterials.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Physical
Bo Chen, Jessica A. Mirrielees, Yuzhi Chen, Timothy B. Onasch, Zhenfa Zhang, Avram Gold, Jason D. Surratt, Yue Zhang, Sarah D. Brooks
Summary: We measured the Tg of IEPOX-derived SOA components using broadband dielectric spectroscopy and found that the Tg of mixtures depends on their composition. The Kwei equation provides a good fit for the Tg-composition relationship of complex mixtures. We demonstrate that the non-linear deviation of Tg as a function of composition may be caused by changes in the extent of hydrogen bonding in the mixture using Raman spectroscopy and density functional theory.
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Biotechnology & Applied Microbiology
Deniz Durmusoglu, Ibrahim Al'Abri, Zidan Li, Taufika Islam Williams, Leonard B. Collins, Jose L. Martinez, Nathan Crook
Summary: This study successfully improved the protein secretion capacity of the probiotic yeast Saccharomyces boulardii (Sb) by manipulating both the genome and expression cassette. Through modulation of expression cassette copy number and genetic modifications, including single and combinatorial gene deletions, a quadruple protease-deficient Sb strain was constructed, which achieved more than tenfold improvement in secretory protein production compared to wild-type Sb. This research provides new tools and strategies for Sb microbial therapy.
MICROBIAL CELL FACTORIES
(2023)
Correction
Chemistry, Multidisciplinary
David O. De Haan, Lelia N. Hawkins, Praveen D. Wickremasinghe, Alyssa D. Andretta, Juliette R. Dignum, Audrey C. De Haan, Hannah G. Welsh, Elyse A. Pennington, Tianqu Cui, Jason D. Surratt, Mathieu Cazaunau, Edouard Pangui, Jean-Francois Doussin
ACS EARTH AND SPACE CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
David O. De Haan, Lelia N. Hawkins, Praveen D. Wickremasinghe, Alyssa D. Andretta, Juliette R. Dignum, Audrey C. De Haan, Hannah G. Welsh, Elyse A. Pennington, Tianqu Cui, Jason D. Surratt, Mathieu Cazaunau, Edouard Pangui, Jean-Francois Doussin
Summary: Aqueous-phase dark reactions during the co oxidation of glyoxal and S(IV) were found to be a potential source of brown carbon (BrC), which occurs more slowly in sunlit, sulfite containing solutions. Detectable amounts of BrC in aerosol require an OH radical source and occur most rapidly after a cloud event. Radical-initiated reactions and redox mechanisms play a role in this process. However, the BrC produced is about ten times less light-absorbing than wood smoke BrC at 365 nm.
ACS EARTH AND SPACE CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jie Zhang, Manish Shrivastava, Alla Zelenyuk, Rahul A. Zaveri, Jason D. Surratt, Matthieu Riva, David Bell, Marianne Glasius
Summary: This study investigates the key parameters governing the formation of isoprene epoxydiol-derived secondary organic aerosol (IEPOX-SOA). The results show that reducing the reaction rate constants of 2-methyltetrol (tetrol) can bring the model predictions in agreement with experimental measurements of IEPOX-SOA under acidified aerosol conditions. Additionally, both the organosulfate (OS) and tetrol reaction rate constants need to be reduced for nonacidified aerosols to match the chamber observations. The study also reveals that the aerosol acidity significantly affects the oligomerization rate of tetrols.
ACS EARTH AND SPACE CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Taufika Islam Williams, Cara Kowalchyk, Leonard B. Collins, Benjamin J. Reading
Summary: Mass spectrometry plays an important role in quantification-centered protein research, and protein cleavage isotope dilution MS is a reliable method for quantifying proteins. In our study, we compared data-dependent acquisition (DDA) and parallel reaction monitoring (PRM) workflows for quantifying egg yolk protein precursors or vitellogenins (VTGs) in striped bass. Our results show that there is no statistical difference between MS1-based and MS2-based VTG quantification.
Review
Environmental Sciences
Sahir Gagan, Kumar Sarang, Krzysztof J. Rudzinski, Ruizhe Liu, Rafal Szmigielski, Yue Zhang
Summary: This review summarizes the progress made in studying the formation of SOA from biogenic VOCs, provides the latest methods for synthesizing marker compounds, and details the oxidation reaction pathways of each BVOC. It offers future directions for connecting atmospheric sciences with organic chemistry.
ATMOSPHERIC ENVIRONMENT
(2023)
Article
Engineering, Environmental
Clara M. A. Eichler, Naomi Y. Chang, Elaine A. Cohen Hubal, Daniel E. Amparo, Jiaqi Zhou, Jason D. Surratt, Glenn C. Morrison, Barbara J. Turpin
Summary: During the Indoor PFAS Assessment (IPA) Campaign, concentrations of nine neutral PFAS were measured in air and cotton cloth in 11 homes in North Carolina. The study found that fluorotelomer alcohols were the dominant species in indoor air, while perfluorooctane sulfonamidoethanols accumulated most significantly in cloth. Cloth-air partition coefficients were derived for different PFAS and were positively correlated with the octanol-air partition coefficient. Temperature was found to have the greatest effect on PFAS accumulation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Chemical
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti
Summary: This study presents the development and validation of a novel adsorbent, PichiaGuard, which is functionalized with peptide ligands and targets the whole spectrum of K. phaffii HCPs for protein purification. The PichiaGuard adsorbent has high HCP binding capacity and successfully purified monoclonal antibodies and fragments, showing its potential for modern biopharmaceutical processing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Environmental Sciences
Daniel J. J. Bryant, Alfred W. W. Mayhew, Kelly L. L. Pereira, Sri Hapsari Budisulistiorini, Connor Prior, William Unsworth, David O. O. Topping, Andrew R. R. Rickard, Jacqueline F. F. Hamilton
Summary: This study proposes a quantification method based on the prediction of relative ionisation efficiency factors to correct the concentrations of biogenic secondary organic aerosol species. The method was developed using commercially available standards and was able to predict the ionisation efficiency factors of biogenic organic acids without authentic standards. The concentration of biogenic secondary organic aerosol was corrected using the predicted factors and resulted in a significant decrease in average concentration.
ENVIRONMENTAL SCIENCE-ATMOSPHERES
(2023)