Article
Chemistry, Multidisciplinary
Chenshuang Zhang, Fangrui Lin, Yong Zhang, Haozhi Yang, Danying Lin, Jun He, Changrui Liao, Xiaoyu Weng, Liwei Liu, Yiping Wang, Bin Yu, Junle Qu
Summary: MSIM-SHG is a novel approach that combines second-harmonic generation (SHG) with multifocal structured illumination microscopy (MSIM) to achieve super-resolution imaging. It has wide applications in imaging material molecules and quantifying collagen fiber alignment, showing potential for clinical diagnosis and biological research.
Article
Optics
Junjun Ma, Fei Xie, Weijin Chen, Jiaxin Chen, Wei Wu, Wei Liu, Yuntian Chen, Wei Cai, Mengxin Ren, Jingjun Xu
Summary: LN metasurfaces with controllable SHG properties have been experimentally demonstrated, showing distinct enhancements for the SHG efficiency at Mie resonances. By changing geometric parameters and resonances of metasurfaces, authors manage to selectively boost the SHG efficiency at different wavelengths, paving the way for developing novel compact nonlinear light sources with high flexibility for applications like biosensing and optical communications.
LASER & PHOTONICS REVIEWS
(2021)
Article
Optics
Marc Jankowski, Carsten Langrock, Boris Desiatov, Marko Loncar, M. M. Fejer
Summary: We propose a new approach to supercontinuum generation and carrier-envelope-offset detection based on saturated second-order nonlinear interactions in dispersion-engineered nanowaveguides. The technique developed here broadens the interacting harmonics by forming stable bifurcations of the pulse envelopes due to an interplay between phase-mismatch and pump depletion. The scaling laws suggest that future realization based on this approach could enable supercontinuum generation with orders of magnitude less energy than current state-of-the-art devices.
Article
Nanoscience & Nanotechnology
Jicheng Jin, Jian Lu, Bo Zhen
Summary: Second harmonic dipoles generated in a nonlinear photonic crystal slab can be completely nonradiative, resulting in no energy transfer from the fundamental frequency to the second harmonic even when the phase-matching condition is satisfied - a phenomenon known as resonance-forbidden second-harmonic generation. Through numerical simulation, two mechanisms for achieving this phenomenon have been identified: symmetry protection and parameter tuning. The finite-size effect and the topological origin of this phenomenon are also discussed.
Article
Chemistry, Multidisciplinary
Biwei Wu, Keyi Wu, Xuefeng Sun, Weibo Wang, Jiubin Tan
Summary: This paper introduces an imaging technique based on circular dichroism second-harmonic generation (CD-SHG) to characterize the 3D distribution of KTP nanocrystals. The circular dichroism analysis can probe the orientation of KTP samples, and the research results show that the azimuthal angle is related to the excitation polarization, refractive index mismatches, and imaging depth.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yoonsoo Rho, SeokJae Yoo, Daniel B. Durham, DongJun Kang, Andrew M. Minor, Costas P. Grigoropoulos
Summary: Nonlinear optical response is a useful tool for studying the properties of materials, but its weak signal and limited reach make it difficult to probe deep-subwavelength-scale nonlinear optics. In this study, we propose a new method using an SHG-active plasmonic nanotip to achieve efficient second harmonic generation (SHG) nanoscopy. Our simulations suggest that enhancing the nonlinear response of the sample or suppressing the tip's response can lead to a high near-field SHG contrast, providing evidence of quantum mechanical nonlinear energy transfer. Furthermore, this technique can be used to study nanoscale corrosion and other physicochemical phenomena.
Article
Optics
Zelin Hao, Wenwei Liu, Zhancheng Li, Zhi Li, Guangzhou Geng, Yanchun Wang, Hua Cheng, Hammad Ahmed, Xianzhong Chen, Junjie Li, Jianguo Tian, Shuqi Chen
Summary: Metasurfaces have shown great potential in optical wave manipulation but face challenges in multidimensional manipulation of harmonic waves generated by nonlinear metasurfaces. This study presents an efficient design strategy based on the hydrodynamic model of free electron dynamics, allowing for full complex-amplitude modulation of second harmonic generation. The proposed nonlinear metasurfaces demonstrate spin-selective SHG optical vortices with independent topological charges, opening up new possibilities for nonlinear metasurface applications in optical information and multifunctional integration.
LASER & PHOTONICS REVIEWS
(2021)
Article
Mathematics
Yernat M. Assylbekov, Ting Zhou
Summary: In this paper, an inverse boundary value problem of electromagnetism with nonlinear Second Harmonic Generation (SHG) process is considered. The unique determination of the electromagnetic material parameters and the SHG susceptibility parameter of the medium is shown by making electromagnetic measurements on the boundary. The case of interest is when a frequency is fixed.
JOURNAL OF DIFFERENTIAL EQUATIONS
(2021)
Article
Chemistry, Multidisciplinary
Baptiste Grimaud, Maxence Fretaud, Feriel Terras, Antoine Benassy, Karine Duroure, Valerie Bercier, Gaelle Trippe-Allard, Rabei Mohammedi, Thierry Gacoin, Filippo Del Bene, Francois Marquier, Christelle Langevin, Francois Treussart
Summary: This study measured fast axonal transport by tracing the SHG signal of nanoKTP endocytosed by zebrafish larvae brain neurons. The researchers confirmed the feasibility of the method by using different mutants and inhibitors, and accurately monitored subtle alterations in axonal transport in the brain.
Article
Acoustics
V. Krishnadas Kanakambaran, Krishnan Balasubramaniam
Summary: This study confirms the presence of simultaneously propagating dual-mode second-harmonics generated on an aluminium plate excited with a fundamental shear horizontal mode. The two wavemodes separate from each other when the travel length exceeds a minimum cut-off length of 40 lambda, providing important implications for more versatile early state defect detection.
Article
Chemistry, Multidisciplinary
Chao Wu, Xingxing Jiang, Lin Lin, Yilei Hu, Tianhui Wu, Zheshuai Lin, Zhipeng Huang, Mark G. Humphrey, Chi Zhang
Summary: The study presents the synthesis of a polar NLO vanadate Cs4V8O22 (CVO) with excellent optical properties and strong second-harmonic generation effects achieved through hydrothermal synthesis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Nanoscience & Nanotechnology
Sander A. . Mann, Heedong Goh, Andrea Alu
Summary: Enabling strong nonlinear optical phenomena requires carefully designed photonic devices to maximize light-matter interactions. Topology optimization has been widely used in optimizing photonic devices due to its efficiency in dealing with large parameter spaces. However, the application of topology optimization in nonlinear effects in dielectric structures is limited.
Article
Materials Science, Multidisciplinary
Tingting Liu, Xinyuan Fang, Shuyuan Xiao
Summary: Engineering nonlinear optical processes in dielectric nanostructures using chalcogenide phase-change material allows for efficient tuning of second-harmonic generation processes. The nonlinear SHG signal from nanoantennas can be enhanced and modulated by optically induced Mie-type resonances and the amorphous-to-crystalline phase change in GST. By adjusting the refractive index of GST, a maximum modulation depth of up to 540% is demonstrated, showing the potential for control of optical nonlinearity and smart designing of tunable nonlinear optical devices.
Article
Chemistry, Analytical
Clara Gomez-Cruz, Sonia Laguna, Ariadna Bachiller-Pulido, Cristina Quilez, Marina Canadas-Ortega, Ignacio Albert-Smet, Jorge Ripoll, Arrate Munoz-Barrutia
Summary: This manuscript presents a novel architecture of a single-plane illumination microscopy (SPIM) for high-resolution imaging of live processes at a cellular level. The custom-made microscope overcomes limitations of existing techniques, achieving high-speed acquisition capabilities, low phototoxicity, and low mechanical disturbances, and demonstrating excellent imaging performance in microfluidic devices.
Article
Optics
Mathias Hedegaard Kristensen, Peter Kjaer Kristensen, Kjeld Pedersen, Esben Skovsen
Summary: This communication demonstrates a new characterization technique that combines FT spectroscopy and SHG to achieve high spectral resolution with broadband femtosecond laser pulses. By showcasing the capabilities of the method with the strong and narrow exciton resonances of ZnO, FT-SHG offers high reproducibility and resolution within the bandwidth of the input pulse.
OPTICS COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Nicolas Dray, Laure Mancini, Udi Binshtok, Felix Cheysson, Willy Supatto, Pierre Mahou, Sebastien Bedu, Sara Ortica, Emmanuel Than-Trong, Monika Krecsmarik, Sebastien Herbert, Jean-Baptiste Masson, Jean-Yves Tinevez, Gabriel Lang, Emmanuel Beaurepaire, David Sprinzak, Laure Bally-Cuif
Summary: The study shows that neural stem cells coordinate their decision to divide in the adult zebrafish brain using spatiotemporally resolved local feedback signals, including short-range inhibition from transient neural progenitors and a dispersion effect from the dividing NSCs themselves. These signals are linked by lineage progression and control the spatiotemporal distribution of output neurons, generating self-propagating dynamics that maintain NSC population homeostasis.
Article
Biotechnology & Applied Microbiology
Pauline Maury, Erika Porcel, Adrien Mau, Francois Lux, Olivier Tillement, Pierre Mahou, Marie-Claire Schanne-Klein, Sandrine Lacombe
Summary: A reproducible and commercially available 3D cell collagen-based model has been developed for studying the influence of the matrix on nanoagent uptake and radiation effects, offering tunable oxygen concentration and homogeneous cell density. The model allows investigation of hypoxic effects and visualization of living cells in real-time using second harmonic imaging microscopy. The disaggregation of the collagen matrix enables recovery of cells without damage, facilitating single cell analysis and quantification of treatment effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Nanoscience & Nanotechnology
Evan P. Stater, Ali Y. Sonay, Cassidy Hart, Jan Grimm
Summary: Nanoparticles, beyond their engineered functions, can induce specific biological effects, which may have implications for the development and clinical application of nanomedicine.
NATURE NANOTECHNOLOGY
(2021)
Article
Biology
Gert-Jan Bakker, Sarah Weischer, Julia Ferrer Ortas, Judith Heidelin, Volker Andresen, Marcus Beutler, Emmanuel Beaurepaire, Peter Friedl
Summary: Three-photon excitation is an effective method for intravital microscopy in deep regions of the mouse brain. This study analyzes the benefits of high-pulse-energy infrared excitation for in-depth imaging of tumor and bone tissue, and demonstrates the possibility of simultaneous 3- and 4-photon processes.
Article
Endocrinology & Metabolism
Marie Karam, Hadrien Janbon, Guy Malkinson, Isabelle Brunet
Summary: This study investigates the distribution and characteristics of LYVE-1(+) PVMs in the mouse brain. The researchers found that LYVE-1(+) PVMs associate with blood vessels in different patterns depending on vessel diameter and arterio-venous differentiation. The density of LYVE-1(+) PVMs is reduced in an Alzheimer's Disease mouse model in proportion to beta-amyloid deposits. These findings suggest that LYVE-1(+) PVMs play important roles in brain development and regulating brain functions in disease conditions.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Biochemical Research Methods
Minh Son Phan, Katherine Matho, Emmanuel Beaurepaire, Jean Livet, Anatole Chessel
Summary: In this study, a local tridimensional scale metric derived from differential geometry is proposed to characterize and compare axonal and dendritic arbors of neurons. The method, called nAdder, is validated on simulated and real data, demonstrating its effectiveness in characterizing neuronal connectivity patterns and behavioral features.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Optics
Julia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas B. David, Laure Bally-Cuif, Nicolas Dray, Michel Negrerie, Willy Supatto, Emmanuel Beaurepaire
Summary: In this work, a novel approach based on label-free third-order sum-frequency generation (TSFG) and third-harmonic generation (THG) contrasts is introduced to map red blood cells (RBCs) flow and oxygenation. The study shows a strong three-photon (3P) resonance related to the Soret band of hemoglobin in THG and TSFG signals of zebrafish and human RBCs, which is sensitive to RBC oxygenation state. The color TSFG implementation enables specific detection of flowing RBCs with single-cell resolution and microsecond pixel times in zebrafish embryos, and provides label-free RBC-specific contrast at depths exceeding 600 μm in live adult zebrafish brain.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Multidisciplinary Sciences
Josephine Morizet, Nicolas Olivier, Pierre Mahou, Arthur Boutillon, Chiara Stringari, Emmanuel Beaurepaire
Summary: This study experimentally and numerically analyzed the contrast of third harmonic generation (THG) microscopy images from stretched hollow glass pipettes in different liquids. The nonlinear optical properties of 2,2'-thiodiethanol (TDE) were also characterized. The presence of index discontinuity was found to affect the THG signals, including the level, modulation amplitude, and polarization direction. The finite-difference time-domain (FDTD) modeling strategy was shown to accurately account for contrast in optically heterogeneous samples, while reference Fourier-based numerical approaches were only accurate in the absence of index mismatch. This work provides insights for interpreting THG microscopy images of tubular objects and other geometries.
SCIENTIFIC REPORTS
(2023)
Article
Optics
Clothilde Raoux, Anatole Chessel, Pierre Mahou, Gael Latour, Marie-Claire Schanne-Klein
Summary: Using SHG microscopy, this study quantitatively characterizes the multiscale structure of the human cornea, finding that the lamellae made of collagen fibrils change gradually along the depth of the cornea, with the anterior stroma oriented along the inferior-superior axis and the posterior stroma along the nasal-temporal axis, with more disorder in the anterior stroma.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Matthew Gleeson, Josephine Morizet, Pierre Mahou, Michel Daudon, Dominique Bazin, Chiara Stringari, Marie-Claire Schanne-Klein, Emmanuel Beaurepaire
Summary: In this study, a kidney stone component classification scheme based on multiphoton response was introduced. It allows fast and accurate differentiation of different stone compositions and provides detailed information about the stone tissues.
Proceedings Paper
Engineering, Biomedical
Vincent Maioli, Antoine Boniface, Pierre Mahou, Julie Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto
Summary: This study presents a framework for quantifying photodamage in multiphoton light-sheet microscopy, and demonstrates a significant signal enhancement by adjusting the laser repetition rate during cardiac imaging in live zebrafish embryos.
ADVANCES IN MICROSCOPIC IMAGING III
(2021)
