Article
Food Science & Technology
Apinya Prachugsorn, Phuvadol Thanakiatkrai, Kittirat Phooplub, Sirirat Ouiganon, Yossawadee Sriaead, Panote Thavarungkul, Proespichaya Kanatharana, Chittanon Buranachai, Thitika Kitpipit
Summary: A novel direct asymmetric PCR and catalyzed hairpin assembly fluorescent biosensor was developed for naked eye detection of porcine DNA in food. The method showed high sensitivity and specificity, and demonstrated reproducibility. It can be utilized by law enforcement agencies and small-scale laboratories to investigate contamination and adulteration of halal foods.
Article
Chemistry, Analytical
Guo-Xi Liang, Shu-Ying Ye, Hao-Ming Yu, Kai-Ren Zhao, Peng-Fei Liu, Zhi-Jun Liu, Li Wang
Summary: This study proposes a new Zika virus diagnostic platform that combines a DNA walker structure and a localized catalytic hairpin assembly (LCHA) cascade amplification strategy for fast, low-cost, and accurate diagnosis. The method allows for Zika virus sequence detection down to 20 pM without enzymatic DNA amplification, and can detect the virus directly from serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yu Xiong, Jianyuan Dai, Yujiao Zhang, Cuisong Zhou, Hongyan Yuan, Dan Xiao
Summary: This study reported a label-free fluorescent signal amplification system based on CHA, which was successfully used for sensitive detection of HIV DNA and Pb2+. By triggering the CHA reaction to release G-quadruplex sequences and bind ThT dye to provide fluorescence signals, and continuing the CHA cycle to amplify the signal.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Furui Jin, Danke Xu
Summary: The mi-CHA method detects miRNAs by changing the conformation of hairpin structured DNA probes on the chip surface, showing high sensitivity and specificity for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Jinri Chen, Jingzheng Liu, Jingyuan Wang, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: A fast and efficient method was used to select an optimized aptamer, Apt3, for the detection of polysialic acid (PSA) in biological samples and clinical diagnostics.
Article
Biochemical Research Methods
Jiaguang Su, Wenjun Zheng, Yanbin Pan
Summary: We propose a proximity ligated initiated magnesium ion (Mg2+)-dependent DNAzyme-powered signal cascade for sensitive, accurate and reliable detection of miRNAs. The approach includes target miRNA recycle, DNAzyme powered substrate cleavage, and catalytic hairpin reaction (CHA) to achieve signal amplification. This method has a low limit of detection and a wide detection range, and shows high selectivity to target miRNA, indicating its potential future applications in skin cancer diagnosis and recovery evaluation.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Hua Chai, Mingyuan Wang, Longhai Tang, Peng Miao
Summary: An ultrasensitive electrochemical biosensor for miRNA assay was developed in this study, showing remarkable sensitivity and a wide linear range, as well as the capability to specifically discriminate interfering miRNAs with slightly different sequences.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Ye Zhang, Xiaohe Zhang, Bo Situ, Yuan Wu, Shihua Luo, Lei Zheng, Yurong Qiu
Summary: The study introduced a robust electrochemical biosensor using multifunctional DNA tetrahedrons for exo-miRNA analysis, demonstrating high sensitivity and rapid detection. The platform showed efficient quantification of exo-miRNA and profiling of tumor-associated miRNAs in breast cancer, with high sensitivity for breast tumor diagnosis and early detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Jinglan Cao, Xinmei Song, Qirong Chen, Ruo Yuan, Yun Xiang
Summary: In this study, a new amplified electrochemical biosensor was developed for the sensitive assay of Sonic Hedgehog (SHh) protein. The biosensor, based on the combination of a SHh-specific aptamer and primer exchange reaction (PER) and catalytic hairpin assembly (CHA), enables the detection of SHh with a detection limit of 4.1 pM. The biosensor can be utilized for the detection of low concentrations of SHh in human serum diluent, making it a potential tool for disease diagnosis and prognosis evaluation.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Tianjin Xie, Yuxin Liu, Jiali Xie, Yujie Luo, Kai Mao, Chengzhi Huang, Yuanfang Li, Shujun Zhen
Summary: In this study, a high molecular weight and volume DNA dendrimer was used as a new fluorescence anisotropy (FA) amplifier coupled with target-catalyzed hairpin assembly (CHA) for sensitive detection of miRNA-21. The method showed high sensitivity and selectivity and could be used to detect other biomolecules by changing the nucleic acid probe.
Article
Chemistry, Multidisciplinary
Dan Li, Enlai Yang, Zewei Luo, Qiyue Xie, Yixiang Duan
Summary: A universal fluorescent biosensor template mediated by enzymes was developed for rapid pathogen detection based on a strategy involving a three-dimensional DNA walker and catalyzed hairpin assembly reaction. The biosensor demonstrated high sensitivity in bacterial recognition and CHA cycle steps, showing great potential for pathogen detection in global public health and food safety applications.
Article
Chemistry, Analytical
Xiangyu Ma, Yuheng Ma, Onome Ejeromedoghene, Martha Kandawa-Schulz, Wei Song, Yihong Wang
Summary: In this study, we combined the catalytic hairpin assembly (CHA) reaction with the oxygen reduction reaction (ORR) to amplify the electrochemical signal for miRNA-21 determination. By utilizing a probe formed by hairpin DNA and silver ions (Ag+), the catalytic hairpin assembly reaction and in situ electrodeposition of AuNPs were achieved for miRNA-21 detection. Additionally, the concentration of Ag+ in the solution was detected using fluorescence and colorimetry, resulting in more accurate and reliable detection results for miRNA-21.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Analytical
Zewei Luo, Yongxin Li, Pei Zhang, Lu He, Yanting Feng, Yaqiang Feng, Cheng Qian, Yonghui Tian, Yixiang Duan
Summary: Catalytic hairpin assembly (CHA) is a promising molecular amplifier with advantages of high efficiency, flexible design, and mild reaction condition. In recent years, CHA has been coupled with multiple signal generation and signal transduction techniques to develop diversified fluorescent biosensors. The integration of emerging signal amplification techniques and other advanced technologies has further enhanced the application of CHA-based biosensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haimin Zou, Chiliang Lin, Haocheng Zan, Yuyao Hu, Xiaruiyan Xu, Dongsheng Wang, Qiuju Wang, Yaoqi Xie, Chen Zhou
Summary: In this study, a fluorescent aptasensor based on CHA-HCR was developed for rapid and sensitive detection of H. pylori in stool samples. The aptasensor showed a wide detection range and low limit of detection, and the results were highly consistent with the 13C-UBT, highlighting its potential in H. pylori screening and other biomarker analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Multidisciplinary
Liuting Mo, Wanqi He, Ziyi Li, Danlian Liang, Runhong Qin, Mingxiu Mo, Chan Yang, Weiying Lin
Summary: As isothermal, enzyme-free signal amplification strategies, hybridization chain reaction (HCR) and catalytic hairpin assembly (CHA) possess the advantages such as high amplification efficiency, excellent biocompatibility, mild reactions, and easy operation. Therefore, they have been widely applied in DNA-based biosensors for detecting small molecules, nucleic acids, and proteins. In this review, we summarize the recent progress of DNA-based sensors employing typical and advanced HCR and CHA strategies, including branched HCR or CHA, localized HCR or CHA, and cascaded reactions. In addition, the bottlenecks of implementing HCR and CHA in biosensing applications are discussed, such as high background signals, lower amplification efficiency than enzyme-assisted techniques, slow kinetics, poor stability, and internalization of DNA probes in cellular applications.
FRONTIERS IN CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Shu Zhang, Jian Huang, Jingrun Lu, Min Liu, Xi Chen, Shasha Su, Fei Mo, Junsong Zheng
CURRENT MEDICINAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Quanjing Zhu, Haoyang Yang, Jing Luo, Hui Huang, Lichao Fang, Jun Deng, Chenghong Li, Yan Li, Tao Zeng, Junsong Zheng
Summary: A novel 3D matrixed DNA self-nanocatalyzer based on gold nanoparticles was proposed for the ultrasensitive bioassay of DNA 5-methylcytosine, showing potential applications in clinical diagnosis and disease mechanism investigation.
ANALYTICA CHIMICA ACTA
(2021)