Article
Agriculture, Dairy & Animal Science
Kondreddy Eswar Reddy, Minji Kim, Ki Hyun Kim, Sang Yun Ji, Youlchang Baek, Ju Lan Chun, Hyun Jung Jung, Changyong Choe, Hyun Jeong Lee, Minseok Kim, Sung Dae Lee
Summary: The mycotoxins DON and ZEN contaminated in pig feed impacted the cecum microbiota, with Lactobacillus and Bacteroides playing key roles in detoxification and enhancing host physiology. These results suggest that disturbances in intestinal microflora, like those caused by toxic effects of DON and ZEN, could potentially be treated by modulating the bacterial flora.
Article
Food Science & Technology
Veronika Nagl, Bertrand Grenier, Philippe Pinton, Ursula Ruczizka, Maximiliane Dippel, Moritz Buenger, Isabelle P. Oswald, Laura Soler
Summary: This study elucidated the role of Zearalenone (ZEN) as a metabolic disruptor in pigs by investigating changes in energy balance and adipokines levels in response to different treatment diets. Results showed that ZEN impacts lipid and glucose metabolism, with effects varying based on dose and exposure time, leading to alterations in adipokine concentrations.
Article
Food Science & Technology
M. Drouault, C. Delalande, H. Bouraima-Lelong, V. Seguin, D. Garon, V. Hanoux
Summary: Deoxynivalenol (DON), a prevalent mycotoxin, can disrupt estrogen signaling by the ligand-independent activation of ERα after short-term exposure.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Review
Food Science & Technology
Paula Llorens, Marta Herrera, Ana Juan-Garcia, Juan Jose Paya, Juan Carlos Molto, Agustin Arino, Cristina Juan
Summary: This review provides the latest information on ZEN biomarkers in humans, animals, and cell line cultures, highlighting the importance of determining specific biomarkers for estimating dietary intake and characterizing severity of ZEN exposure. The study also reveals the cytotoxic effects of oc-ZEL and beta-ZEL, as well as the oxidative stress effect and membrane integrity changes caused by ZEN exposure.
Article
Multidisciplinary Sciences
Dustin J. Flanagan, Nalle Pentinmikko, Kalle Luopajarvi, Nicky J. Willis, Kathryn Gilroy, Alexander P. Raven, Lynn Mcgarry, Johanna Englund, Anna T. Webb, Sandra Scharaw, Nadia Nasreddin, Michael C. Hodder, Rachel A. Ridgway, Emma Minnee, Nathalie Sphyris, Ella Gilchrist, Arafath K. Najumudeen, Beatrice Romagnolo, Christine Perret, Ann C. Williams, Hans Clevers, Pirjo Nummela, Marianne Lahde, Kari Alitalo, Ville Hietakangas, Ann Hedley, William Clark, Colin Nixon, Kristina Kirschner, E. Yvonne Jones, Ari Ristimaki, Simon J. Leedham, Paul Fish, Jean-Paul Vincent, Pekka Katajisto, Owen J. Sansom
Summary: The tumour suppressor APC is the most commonly mutated gene in colorectal cancer. Loss of Apc in intestinal stem cells drives the formation of adenomas in mice via increased WNT signalling, while reduced secretion of WNT ligands increases the ability of Apc-mutant intestinal stem cells to colonize a crypt. The study found that Apc-mutant cells are enriched for transcripts encoding several secreted WNT antagonists, with Notum being the most highly expressed. These cells actively inhibit the proliferation of surrounding wild-type crypt cells, outcompeting them in the niche and driving their differentiation.
Article
Medicine, Research & Experimental
Zheng Qin, Yupei Li, Jiameng Li, Luojia Jiang, Zhuyun Zhang, Kaixi Chang, Qinbo Yang, Shanshan Chen, Ruoxi Liao, Baihai Su
Summary: This study found that exosomal STAT1 derived from HP-treated HUVECs can promote VSMC calcification. Activation of the Wnt/beta-catenin pathway may be a potential mechanism of exosome-promoted VSMC calcification.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Byeong-Yun Ahn, Myong-Ho Jeong, Jung-Hoon Pyun, Hyeon-Ju Jeong, Tuan Anh Vuong, Ju-Hyeon Bae, Subin An, Su Woo Kim, Yong Kee Kim, Dongryeol Ryu, Hyun-Ji Kim, Hana Cho, Gyu-Un Bae, Jong-Sun Kang
Summary: PRMT7 plays an important role in cardiac hypertrophy and fibrosis. Overexpression of PRMT7 can alleviate cellular hypertrophic response, while depletion of PRMT7 exacerbates hypertrophic response. Transcriptome analysis reveals altered gene expression profile related to Wnt signaling pathway in PRMT7-deficient hearts.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Food Science & Technology
Yang Li, Junfei Chen, Dong Sun, Jingwei Liu, Zhe Wang, Aiwu Li
Summary: LGG administration can alleviate intestinal lesions in NEC by restoring intestinal flora, activating Wnt/β-catenin signaling pathway, promoting ISC regeneration, increasing the number of TJs, and improving intestinal barrier function.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biotechnology & Applied Microbiology
Weiqin Ji, Xiaotong Sun
Summary: MeCP2 promotes osteogenic differentiation of BMSCs by regulating the FOXF1/Wnt/β-Catenin axis to alleviate osteoporosis. MeCP2 overexpression reduces FOXF1 to promote activation of Wnt5a/β-Catenin and enhance osteogenic differentiation of BMSCs in the prevention of postmenopausal osteoporosis.
Review
Biochemistry & Molecular Biology
Gabriele Colozza, So-Yeon Park, Bon-Kyoung Koo
Summary: Tumors gain a competitive edge over intestinal stem cells by exploiting the self-renewal processes of the stem cells. The dynamics of stem cells in the intestines are regulated by a network of molecular signals and competition for limited space. The Wnt signaling pathway and its inhibitors play a crucial role in maintaining the balance between self-renewal and differentiation. Intestinal cancer cells bypass these regulatory mechanisms, allowing them to outcompete normal cells.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Environmental Sciences
Han Song, Junjun Qiu, Keqin Hua
Summary: USP14 is overexpressed in cervical cancer and is associated with clinical stage and prognosis of patients. It promotes the proliferation, migration, and invasion of cervical cancer cells by upregulating the expression of beta-catenin.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
German Belenguer, Gianmarco Mastrogiovanni, Clare Pacini, Zoe Hall, Anna M. Dowbaj, Robert Arnes-Benito, Aleksandra Sljukic, Nicole Prior, Sofia Kakava, Charles R. Bradshaw, Susan Davies, Michele Vacca, Kourosh Saeb-Parsy, Bon-Kyoung Koo, Meritxell Huch
Summary: RNF43/ZNRF3 alterations contribute to liver disease and liver cancer by affecting differentiation and lipid metabolism of hepatocytes. The loss of these genes leads to steatohepatitis and an increase in unsaturated lipids. Deletion of Rnf43/Znrf3 results in defective hepatocyte regeneration and liver cancer, causing an imbalance between differentiation and proliferation. Interestingly, ZNRF3 mutant liver cancer patients have a poorer prognosis and exhibit altered hepatic lipid metabolism and steatohepatitis/NASH signatures.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Su Luo, Chloe Terciolo, Manon Neves, Sylvie Puel, Claire Naylies, Yannick Lippi, Philippe Pinton, Isabelle P. Oswald
Summary: This study demonstrated that proliferative intestinal epithelial cells are more susceptible to the mycotoxin deoxynivalenol (DON) compared to differentiated cells. DON was also found to delay the differentiation process in the intestinal epithelium. The differential gene expression analysis revealed the involvement of various pathways in the response to DON and the differentiation process.
ENVIRONMENTAL POLLUTION
(2021)
Article
Food Science & Technology
Clemence Budin, Hai-Yen Man, Carine Al-Ayoubi, Sylvie Puel, Barbara M. A. van Vugt-Lussenburg, Abraham Brouwer, Isabelle P. Oswald, Bart van der Burg, Laura Soler
Summary: Aflatoxins, particularly AFB1, have major adverse effects on human health by becoming a potent carcinogen once converted into a DNA-reactive form. This study found that VerA and AFB1 have similar cytotoxic effects and genotoxicity, with VerA also activating the aryl hydrocarbon receptor to enhance AFB1 genotoxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marie Tremblay-Franco, Cecile Canlet, Philippe Pinton, Yannick Lippi, Roselyne Gautier, Claire Naylies, Manon Neves, Isabelle P. Oswald, Laurent Debrauwer, Imourana Alassane-Kpembi
Summary: The effects of low doses of toxicants are subtle and metabolomic data alone may not be sufficient. Integrating metabolomic and transcriptomic data can increase the knowledge extracted from toxicant-induced disruptions, revealing more discriminant metabolites and other types of damage linked to the exposure.
Article
Nutrition & Dietetics
Delphine Payros, Marion Garofalo, Alix Pierron, Laura Soler-Vasco, Carine Al-Ayoubi, Viviane M. Maruo, Imourana Alassane-Kpembi, Philippe Pinton, Isabelle P. Oswald
Summary: Human exposure to mycotoxins through diet is a growing concern, with regulations established for seven mycotoxins in Europe. New secondary fungal metabolites and mixtures present challenges for research in this field.
CAHIERS DE NUTRITION ET DE DIETETIQUE
(2021)
Article
Environmental Sciences
Alix Pierron, Manon Neves, Sylvie Puel, Yannick Lippi, Laura Soler, J. David Miller, Isabelle P. Oswald
Summary: The study revealed that NX, DON, and 3ANX have similar impacts on the intestine, mainly involving cell proliferation, differentiation, apoptosis, growth, immune and pro-inflammatory responses, with NX showing greater transcriptional impacts compared to DON.
Article
Food Science & Technology
Barbara Novak, Amanda Lopes Hasuda, Mahdi Ghanbari, Viviane Mayumi Maruo, Ana Paula F. R. L. Bracarense, Manon Neves, Caroline Emsenhuber, Silvia Wein, Isabelle P. Oswald, Philippe Pinton, Dian Schatzmayr
Summary: Research showed that the co-application of EB and DON resulted in reduced weight gain, decreased liver enzyme activities, and histological lesions in various organs of piglets; whereas EB application significantly affected the gut microbiota. These findings provide new insights into the detrimental effects of emerging mycotoxins on pigs.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Food Science & Technology
Leonie Dopavogui, Arnaud Polizzi, Anne Fougerat, Pascal Gourbeyre, Chloe Terciolo, Wendy Klement, Philippe Pinton, Joelle Laffite, Anne-Marie Cossalter, Jean-Denis Bailly, Olivier Puel, Yannick Lippi, Claire Naylies, Herve Guillou, Isabelle P. Oswald, Nicolas Loiseau
Summary: Fumonisin B1 (FB1) is a widespread mycotoxin produced mainly in maize, and pigs and horses are most susceptible to it. FB1 exposure can cause diverse clinical symptoms. By analyzing the transcriptomes of four organs, common biological processes and tissue-specific processes related to lipid outcomes were identified in response to FB1 exposure.
Article
Food Science & Technology
Amanda Lopes Hasuda, Elodie Person, Abdullah Khan Khoshal, Sandrine Bruel, Sylvie Puel, Isabelle P. Oswald, Ana Paula F. R. L. Bracarense, Philippe Pinton
Summary: Research indicates that Deoxynivalenol has toxic effects on liver cell lines and whole liver tissues, with the pig precision-cut liver slices model being a relevant and sensitive model for studying the liver toxicity of food contaminants.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Marion Garofalo, Delphine Payros, Eric Oswald, Jean-Philippe Nougayrede, Isabelle P. Oswald
Summary: This study found that the dose of DON found in food can exacerbate DNA damage caused by etoposide, cisplatin, and phleomycin, but not the DNA damage caused by de-epoxy-deoxynivalenol (DOM-1). Ribotoxicity plays a key role in exacerbating DNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Toxicology
Laura Soler, Ingrid Miller, Chloe Terciolo, Karin Hummel, Katharina Noebauer, Manon Neves, Isabelle P. Oswald
Summary: We compared the protein composition of pig intestinal explants exposed to DON or NX and found that the changes in the extracellular proteome were different between the two toxins. DON decreased cell viability and caused cell destruction, while NX led to an enrichment of mitochondrial proteins in the secretome, which may be related to its ability to induce intestinal inflammation.
ARCHIVES OF TOXICOLOGY
(2022)
Editorial Material
Mycology
Olivier Puel, Benedikt Cramer, Isabelle P. Oswald, Hans-Ulrich Humpf
MYCOTOXIN RESEARCH
(2022)
Article
Food Science & Technology
Carine Al-Ayoubi, Justin Oules, Elodie Person, Sandrine Bruel, Alyssa Bouville, Philippe Pinton, Isabelle P. Oswald, Emilien L. Jamin, Olivier Puel, Laura Soler
Summary: This study generated a panel of metabolites derived from in-house purified VerA, suggesting the bioactivation of VerA into an epoxide form. These qualitative analytic data provide valuable information for further precise metabolism study of VerA.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
I. Oswald