Article
Cell Biology
Muhammad Ali Khan, Purba Nag, Anca Grivei, Kurt T. K. Giuliani, Xiangju Wang, Vishal Diwan, Wendy Hoy, Helen Healy, Glenda Gobe, Andrew J. Kassianos
Summary: The pathogenesis of crystal nephropathy involves deposition of intratubular crystals, tubular obstruction and cell death. This study investigated the modes of adenine-induced tubular cell death and found that baicalein can act as a novel cell death inhibitor. Baicalein inhibits cell death by modulating the mitochondrial antioxidant enzyme SOD2.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Yang Chen, Hui-Yi Li, Jian-Shu Liu, Dao-long Jiang, Hao-nan Zheng, Xue-Song Dong
Summary: In this study, a diquat-induced human kidney-2 cells (HK-2 cells) apoptosis model was established and differentially expressed miRNAs and signaling pathways involved in diquat poisoning were identified through gene sequencing and bioinformatics analysis. The findings contribute to understanding the mechanisms of diquat poisoning and provide new targets for gene therapy of related diseases.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Timo N. Haschler, Harry Horsley, Monika Balys, Glenn Anderson, Jan-Willem Taanman, Robert J. Unwin, Jill T. Norman
Summary: SIRT5 plays a critical role in ischemic renal injury, with its depletion affecting mitochondrial function in murine and human proximal tubular epithelial cells.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Coral Garcia-Pastor, Selma Benito-Martinez, Ricardo J. Bosch, Ana B. Fernandez-Martinez, Francisco J. Lucio-Cazana
Summary: Proximal tubular cells (PTC) are vulnerable to hypoxia-induced apoptosis, which may be a crucial factor in kidney diseases. Research has shown that apoptosis under hypoxia is mediated by COX-2-dependent production of PGE(2), suggesting potential new therapeutic targets for preventing hypoxia-dependent lesions in renal diseases.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Parva R. Thakker, Ardeshir J. Ariana, Stephanie R. Hajjar, David J. Cai, Dikchha R. Rijal, Subash J. Sad
Summary: XIAP is an endogenous inhibitor of cell death and inactivating mutations of XIAP are responsible for X-linked lymphoproliferative disease (XLP-2) and primary immunodeficiency. This study demonstrates that XIAP plays a crucial role in controlling cell death and regulating cytokine secretion during infection with intracellular bacteria. XIAP promotes the fitness and survival of activated CD8 T cells, but its deficiency leads to poor expansion, skewed differentiation, and a failure to control bacterial infection.
Review
Endocrinology & Metabolism
Shuang Shen, Chuanyuan Ji, Kaifeng Wei
Summary: Cellular senescence plays a crucial role in chronic diseases, causing irreversible cell proliferation arrest, increased protein production, resistance to apoptosis, and altered metabolic activity. Diabetic tubulopathy is closely linked to hyperglycemia, oxidative stress, chronic inflammation, glucose toxicity, accumulation of advanced glycation end-products, lipid metabolism disorders, and lipotoxicity. Understanding the cellular senescence and various patterns of regulated cell death in tubular epithelial cells provides new insights into the pathogenesis of diabetic kidney disease (DKD).
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Ali Rafat, Khadijeh Dizaji Asl, Zeinab Mazloumi, Ali Akbar Movassaghpour, Mehdi Talebi, Dariush Shanehbandi, Raheleh Farahzadi, Babak Nejati, Hojjatollah Nozad Charoudeh
Summary: The study revealed that Telomerase Inhibition (TI) induced apoptosis through intrinsic, extrinsic, and p53 pathways and increased the expression of DNMT3a and TET2 epigenetic markers.
Article
Immunology
Lisa Cucolo, Qingzhou Chen, Jingya Qiu, Yongjun Yu, Max Klapholz, Krista A. Budinich, Zhaojun Zhang, Yue Shao, Igor E. Brodsky, Martha S. Jordan, D. Gary Gilliland, Nancy R. Zhang, Junwei Shi, Andy J. Minn
Summary: Interferon-gamma has various effects on cancer immune checkpoint blockade, including its role in resistance. The study found that the expression of Ripk1 gene is strongly associated with interferon-mediated resistance. RIPK1 diverts TNF signaling in cancer cells, leading to the production of immunosuppressive chemokines and decreased expression of TNF superfamily ligands in T and NK cells.
Article
Engineering, Environmental
Shuo Zhang, Xiaona Zhao, Jiajia Hao, Yiran Zhu, Yue Wang, Lumei Wang, Shuhua Guo, Hui Yi, Yongxia Liu, Jianzhu Liu
Summary: The study revealed that hexavalent chromium could induce apoptosis in DF-1 cells, affecting the mitochondrial membrane potential and causing endoplasmic reticulum stress. Silencing ATF-6 reduced apoptosis rate and ER stress, influencing the expression of apoptosis-related proteins.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Yanbing Zhao, Huan Zhang, Dezheng Hao, Jingqiu Wang, Ruixin Zhu, Weina Liu, Ci Liu
Summary: This study found that selenium-rich yeast has a protective effect on the function and apoptosis of broiler kidney tissues induced by hexavalent chromium. The mechanism may be related to the regulation of the mitogen-activated protein kinase pathway activated by oxidative stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Cell Biology
Alexandra Linke, Hakan Cicek, Anne Mueller, Catherine Meyer-Schwesinger, Simon Melderis, Thorsten Wiech, Claudia Wegscheid, Julius Ridder, Oliver M. Steinmetz, Linda Diehl, Gisa Tiegs, Katrin Neumann
Summary: This study found that proximal tubular epithelial cells (PTECs) have the capacity for antigen cross-presentation, which can modulate the immune response in immune-mediated glomerular diseases, such as lupus nephritis.
Article
Engineering, Chemical
Subham Sharma, Kyu-Jae Lee, Johny Bajgai, Thuy Thi Trinh, Jayson M. Antonio, Md Habibur Rahman, Kchorng Vira, Abdul-Nasir Sofian, Syung Hyun Cho, Cheol-Su Kim, Yundeok Kim
Summary: This study investigates the anti-oxidative and anti-diabetic effects of electrolyzed weakly alkaline reduced water (EARW) at pH 8.5 on human kidney-2 (HK-2) cells. The results show that EARW at pH 8.5 rescues cell viability and reduces oxidative stress and inflammation markers in HK-2 cells induced with high glucose (HG). Moreover, EARW at pH 8.5 enhances antioxidant defenses and improves cellular metabolism in HG-induced HK-2 cells.
Article
Cell Biology
Jennifer Eymael, Brigith Willemsen, Joyce Xu, Fieke Mooren, Eric Steenbergen, Jack F. Wetzels, Henry Dijkman, Jitske Jansen, Johan van der Vlag, Bart Smeets
Summary: Multiple motile cilia with a central microtubular pair were found in kidney biopsies of patients with kidney diseases, and these cells were associated with tubular injury and interstitial fibrosis. This suggests the ability of proximal tubular cells to transdifferentiate into multiciliated cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Tao Wu, Qiang Xiong, Ranran Song, Qingjiang Wang, Fan Zhang, Pingang He
Summary: An Au-Cu dual-electrode tip was designed to monitor the effect of Cu2+ on the membrane permeability of a single living cell using scanning electrochemical microscopy. By obtaining probe approach curves (PACs) and theoretical values, the cell membrane permeability coefficient (P-m) values were determined. The direct generation of Cu2+ by electrolysis was used to investigate its impact on cell membrane permeability in situ, providing insights into the mechanism of acute heavy metal damage on cell membranes and the role of heavy metal ions in physiological or pathological processes.
Article
Environmental Sciences
XiangFei Zhang, YuTing Wang, Ming Chen, Ming Zeng
Summary: Studies have shown that hexavalent chromium can induce p53-independent apoptosis through calcium overload. The activation of the Ca2+/CaM/CaMKII signaling pathway and changes in apoptosis-associated factors play important roles in this process.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Emergency Medicine
Ming-Chan Wu, Yen-Hung Wu, Pin-Han Wang
JOURNAL OF EMERGENCY MEDICINE
(2020)
Article
Medicine, General & Internal
I-Jeng Yeh, Kuan-Ting Liu, Jheng-Heng Shen, Yen-Hung Wu, Yao-Hua Liu, Meng-Chi Yen, Po-Lin Kuo
Article
Cell Biology
Pin-Shern Chen, Hui-Ping Hsu, Nam Nhut Phan, Meng-Chi Yen, Feng-Wei Chen, Yu-Wei Liu, Fang-Ping Lin, Sheng-Yao Feng, Tsung-Lin Cheng, Pei-Hsiang Yeh, Hany A. Omar, Zhengda Sun, Jia-Zhen Jiang, Yi-Shin Chan, Ming-Derg Lai, Chih-Yang Wang, Jui-Hsiang Hung
Summary: Breast cancer accounts for 30% of new cancer cases diagnosed in American women, and high expression levels of CCDC167 predict poor prognoses of breast cancer patients. Knockdown of CCDC167 attenuates aggressive breast cancer growth and proliferation. Treatment with certain drugs can decrease CCDC167 expression and suppress the growth of breast cancer cells.
Article
Medicine, General & Internal
Li-Chin Cheng, Tzu-Jen Kao, Nam Nhut Phan, Chung-Chieh Chiao, Meng-Chi Yen, Chien-Fu Chen, Jui-Hsiang Hung, Jia-Zhen Jiang, Zhengda Sun, Chih-Yang Wang, Hui-Ping Hsu
Summary: This study utilized a holistic bioinformatics approach to study gene signatures of SARS-CoV and SARS-CoV-2 infected cells, revealing up-regulation of several cytokine genes and differential regulation of pathways related to chemokines, cytokines, inflammation, and immune responses. The analysis also showed enrichment of TNF- and IL-17-related signaling and predicted downstream networks involved in leukocyte recruitment, activation, and survival after coronavirus infection. These findings provide important evidence for transcript regulation and downstream signaling in SARS-CoV and SARS-CoV-2 infections.
Article
Immunology
Yen-Hung Wu, I-Jeng Yeh, Nam Nhut Phan, Meng-Chi Yen, Jui-Hsiang Hung, Chung-Chieh Chiao, Chien-Fu Chen, Zhengda Sun, Hui-Ping Hsu, Chih-Yang Wang, Ming-Derg Lai
Summary: By integrating miRNA and mRNA expression profiles, we identified 251 upregulated genes and eight highly differentiated miRNAs in MERS-CoV-infected cells. We revealed immune, olfactory/sensory system-related, and signal-transduction networks associated with MERS-CoV infection. Using the Connectivity Map platform, potential compounds against MERS-CoV infection were suggested.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Biology
Meng-Chi Yen, Kuan-Li Wu, Yu-Wei Liu, Yung-Yun Chang, Chao-Yuan Chang, Jen-Yu Hung, Ying-Ming Tsai, Ya-Ling Hsu
Summary: The study identified a high expression of UBE2H in metastatic malignant pleural tumors, which was associated with poor survival in patients with LUAD. Inhibiting UBE2H could suppress migration capacity of LUAD cells and reverse the epithelial-mesenchymal transition signaling pathway. Certain microRNAs targeting UBE2H may serve as potential prognostic biomarkers for survival in LUAD.
Editorial Material
Emergency Medicine
Chi-Wei Chen, Yen-Yi Lee, Yen-Hung Wu
EMERGENCY MEDICINE JOURNAL
(2021)
Article
Immunology
Do Thi Minh Xuan, I-Jeng Yeh, Chung-Che Wu, Che-Yu Su, Hsin-Liang Liu, Chung-Chieh Chiao, Su-Chi Ku, Jia-Zhen Jiang, Zhengda Sun, Hoang Dang Khoa Ta, Gangga Anuraga, Chih-Yang Wang, Meng-Chi Yen
Summary: Monkeypox virus infection is characterized by elevated expression of genes associated with interleukins, G protein-coupled receptors, heat shock proteins, Toll-like receptors, and metabolic-related pathways. The dominant factors in monkey MK2 cell line model are CD40, plasmin, and histamine, while interferons, macrophages, and neutrophil-related signaling pathways dominate in human HeLa cell line model.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Medicine, General & Internal
Do Thi Minh Xuan, I-Jeng Yeh, Che-Yu Su, Hsin-Liang Liu, Hoang Dang Khoa Ta, Gangga Anuraga, Chung-Chieh Chiao, Chih-Yang Wang, Meng-Chi Yen
Summary: By integrating multiple databases and tools, this study found that PSMG family members were highly expressed in lung adenocarcinoma (LUAD) patients and correlated with poor prognosis. Furthermore, PSMG family genes were also positively associated with immune response, ubiquinone metabolism, cell cycle regulation, and HSP90/PI3K/Wnt signaling. Experimental data confirmed the impact of PSMG4 knockdown on LUAD cell proliferation and downstream molecule expressions. These findings suggest that PSMG family members could serve as novel prognostic biomarkers for LUAD progression and potential therapeutic targets.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Oncology
Ahmad Raza, Meng-Chi Yen, Gangga Anuraga, Iram Shahzadi, Muhammad Waqar Mazhar, Hoang Dang Khoa Ta, Do Thi Minh Xuan, Sanskriti Dey, Sachin Kumar, Adrian Wangsawijaya Santoso, Bianca Tobias William, Chih-Yang Wang
Summary: This study aimed to find a prognostic biomarker for GBM treatment. By analyzing data from the TCGA database, it was found that GNAI3 is associated with poor prognosis in GBM and is involved in important pathways such as macrophage maturation and cytoskeleton arrangements. These findings suggest that GNAI3 may serve as a valuable prognostic biomarker for the treatment of GBM.
Article
Cell Biology
Su-Chi Ku, Hsin-Liang Liu, Che-Yu Su, I-Jeng Yeh, Meng-Chi Yen, Gangga Anuraga, Hoang Dang Khoa Ta, Chung-Chieh Chiao, Do Thi Minh Xuan, Fidelia Berenice Prayugo, Wei-Jan Wang, Chih-Yang Wang
Summary: The study found that mRNA levels of CDH1/2/4/11/12/13 genes were overexpressed in breast cancer cells compared to normal cells and correlated with poor prognosis in breast cancer patients. The high expressions of these genes were significantly associated with cell adhesion, extracellular matrix remodeling, EMT, WNT/beta-catenin, and interleukin-mediated immune responses, suggesting they could be potential biomarkers for breast cancer progression and metastasis.
Article
Medicine, General & Internal
Yen-Hung Wu, I-Jeng Yeh, Nam Nhut Phan, Meng-Chi Yen, Hsin-Liang Liu, Chih-Yang Wang, Hui-Ping Hsu
Summary: This study utilized a holistic bioinformatics approach to explore gene signature alterations in SARS-CoV-2-infected lung epithelial cells, identifying enrichment of genes associated with immune-related pathways, host cell responses, steroid hormone biosynthesis, receptor signaling, and the complement system. Dysregulation of the immune system and interferon malfunction contribute to the failure to eliminate SARS-CoV-2 and worsen respiratory distress in severely ill patients, highlighting the importance of understanding the genetic targets for this infection.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Letter
Medicine, Research & Experimental
Yang-Yi Chen, Cheng-Che E. Lan, Meng-Chi Yen, Shih-Tsung Cheng
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
(2021)