Article
Fisheries
Zhenguo Hu, Weiwei Zhang, Weikang Liang, Zhen Zhang, Ming Guo, Chenghua Li
Summary: A bacterium identified as Bacillus cereus LS2 isolated from healthy sea cucumbers showed strong antagonistic activity against Vibrio splendidus, potentially through the production of a lipopeptide. The antagonistic substance had a molecular weight less than 3 kDa, was sensitive to trypsin but stable at high temperatures, and could be precipitated by acid and dissolved in anhydrous methanol.
Article
Fisheries
Ya Li, Huirong Yang, Jinxia Zhang, Weibo Shi, Weisheng Li, Weiwei Zhang
Summary: This study focused on Vibrio splendidus, a marine pathogen that infects various aquaculture animals. The researchers cloned the catalytic domain of the vspC gene and successfully purified the recombinant catalytic domain rVspC. They found that rVspC can degrade collagen from the body wall and coelomocytes of Apostichopus japonicus. Transcriptomics analysis revealed gene expression changes in A. japonicus injected with rVspC, including upregulation of MMP-14 and downregulation of laminin. Immune-related pathways were affected, with downregulation of apoptosis, lysosome, phagosome, ubiquitin-mediated proteolysis, and antigen processing and presentation, and upregulation of endocytosis. The study also identified a regulatory pathway of Hfq-SmcR-VspC at high cell density of V. splendidus, indicating the positive regulation of SmcR by Hfq and its role in the expression of the vspC gene. Furthermore, the comparative analysis of VspC's catalytic domain sequences suggests the widespread presence of collagenase homologs in bacteria, potentially playing important roles in colonization, environmental adaptation, and virulence.
Article
Fisheries
Fangyuan Zhou, Lianlian Sun, Yina Shao, Xiumei Zhang, Chenghua Li
Summary: Glutaminolysis plays an important role in the immune response in Apostichopus japonicus. Decreased levels of glutaminolysis metabolic substrates and products were observed, accompanied by an increase in glutathione. The key genes in glutaminolysis were upregulated in response to pathogen infection. AMPK beta can regulate glutaminolysis by influencing the uptake of glutamine. Silencing AjAMPK beta leads to increased levels of glutamine and glutamate, decreased production of glutathione, and a reduction in redox homeostasis indexes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Kaiyu Chen, Yina Shao, Chenghua Li
Summary: The sea cucumber AjULK was found to be significantly upregulated after pathogen challenge and involved in autophagy regulation along with other autophagy-related genes. Knockdown of AjULK markedly inhibited the autophagy signal and affected protein levels inside cells, ultimately restricting bacterial invasion.
Article
Fisheries
Yingfen Dai, Zhimeng Lv, Meixiang You, Lianlian Sun, Chenghua Li
Summary: Organisms trigger pro-inflammatory responses to resist foreign pathogens, but excessive or chronic inflammation can cause diseases. This study found that the PPAR alpha gene in sea cucumbers was significantly induced at the late stage of infection and negatively correlated with AjIL-17 and AjNLRP3 expression. The results supported that AjPPAR alpha exerted anti-inflammatory effects through inhibiting AjRel.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Wenyang Ma, Ya Li, Weibo Shi, Weiwei Zhang, Qingxi Han
Summary: This study investigated the role of Ajpacifastin-like in regulating the immune response in the sea cucumber. It was found that Ajpacifastin-like can inhibit bacterial growth and regulate multiple immune pathways. Transcriptome analysis identified differentially expressed genes, providing further insights into the function of Ajpacifastin-like.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Yue Shi, Changyu Liao, Fa Dai, Yiwei Zhang, Chenghua Li, Weikang Liang
Summary: Vibrio splendidus is an opportunistic pathogen that causes significant losses to the Apostichopus japonicus breeding industry. By investigating the role of the Vsfur gene in biofilm, swarming motility, and virulence, it was found that Vsfur contributes to the pathogenesis of V. splendidus.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Weikang Liang, Weiwei Zhang, Chenghua Li
Summary: In this study, the pathogenesis of Vibrio splendidus was investigated using mutant strains with an hppDV.s. deletion. The results showed that hppDV.s. plays a crucial role in amino acid metabolism, protein binding, glutamate metabolism, ATP production, flagellum assembly, swarming motility, biofilm formation, and colonization abilities of V. splendidus.
Article
Biology
Jiqing Liu, Yina Shao, Dongdong Li, Chenghua Li
Summary: This study reveals that N6-methyladenosine (m6A) modification regulates autophagy in Apostichopus japonicus during Vibrio splendidus infection. The AjULK-AjYTHDF/AjEEF-1 alpha signaling pathway is involved in m6A-mediated resistance against V. splendidus infection. These findings provide a theoretical basis for disease prevention and therapy in A. japonicus.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Tanjun Zhao, Liyuan Ren, Chengda Li, Li Liu, Yang Zou, Hanyu Yan, Yaoyao Zhan, Yaqing Chang
Summary: This study reveals the regulatory role of miR-7 in the pathogen-induced immune response in A. japonicus by targeting PAK1, highlighting its importance in immune regulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Ya Li, Fa Dai, Yanan Li, Weikang Liang, Chenghua Li, Weiwei Zhang
Summary: Hfq, as an RNA-binding protein, plays a crucial role in regulating the virulence of Vibrio splendidus by affecting adhesion, biofilm formation, and swimming motility through the flagellar assembly and chemotaxis systems. The construction of a Delta hfq mutant strain revealed reduced growth and virulence levels, with transcriptomic analysis showing significant down-regulation of key genes in these pathways. The study highlights the importance of Hfq in modulating virulence-related factors in V. splendidus.
Article
Biochemistry & Molecular Biology
Liyuan Guo, Zhenhui Wang, Weibo Shi, Yinan Wang, Qiang Li
Summary: The study demonstrated the role and immune mechanisms of the Polian vesicle in response to pathogen infection in A. japonicus through comparative transcriptome analysis. It identified key metabolic pathways and gene expression patterns associated with immune response, providing new insights into the hematopoietic function of the Polian vesicle.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Fisheries
Xuda Wang, Jingwei Jiang, Xiaoyan Guan, Zelong Zhao, Ying Dong, Jian Wang, Shilei Li, Bei Jiang, Guiying Liu, Hongjuan Sun, Shan Gao, Pingzhe Jiang, Xiaoyan Wang, Zunchun Zhou
Summary: The combination of dietary Lactobacillus acidophilus and Tussah Immunoreactive Substances (TIS) can significantly enhance the disease resistance of sea cucumbers against Vibrio splendidus by improving digestive and immune parameters and regulating gut microbiota composition.
AQUACULTURE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yuan Ren, Yong-ping Xu, Xu-yuan Fan, Bilal Murtaza, Yi-nan Wang, Zhen Li, Muhammad Tariq Javed, Zhen-hui Wang, Qiang Li
Summary: This study investigated the early functions of polian vesicles in response to pathogenic infection in A. japonicus. Transcriptome sequencing revealed differentially expressed genes and enrichment of signaling pathways related to cell proliferation. The analysis suggests that polian vesicles play a crucial role in regulating proliferation through transcription factor-mediated signaling pathways.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Article
Microbiology
Ya Li, Weibo Shi, Weiwei Zhang
Summary: This study analyzed the effects of exogenous L-Glu on the viability and tissue status of sea cucumbers, and found that high concentrations of L-Glu resulted in cell death and tissue damage, while low concentrations had no significant impact on the survival of sea cucumbers. Transcriptomic analysis revealed that exogenous L-Glu affected the expression of multiple genes, and coexistence of exogenous L-Glu and V. splendidus further increased sea cucumber mortality.
