Article
Cell Biology
Kayo Machihara, Sou Kageyama, Shoma Oki, Hiroki Makino, Masamichi Sasaki, Hiroyasu Iwahashi, Takushi Namba
Summary: Cell aging leads to reduced cellular functions and disruption of cellular homeostasis, with mitochondrial dysfunction playing a crucial role. The study demonstrates that lotus germ extract can rejuvenate aging fibroblasts by restoring mitochondrial function and clearing abnormal proteins.
Article
Cell Biology
Andreas Zimmermann, Corina Madreiter-Sokolowski, Sarah Stryeck, Mahmoud Abdellatif
Summary: Global human life expectancy is on the rise, leading to an increase in age-related chronic diseases, which pose a significant medical and economic burden on society. Current strategies to delay aging and related diseases primarily target mitochondria and protein homeostasis. The focus is on autophagy, a fundamental process linked to mitochondrial quality control, with interventions showing promise in extending health and lifespan.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun-Hao Wen, Xiang-Hong He, Ze-Sen Feng, Dong-Yi Li, Ji-Xin Tang, Hua-Feng Liu
Summary: The accumulation of protein aggregates is a characteristic feature of neurodegenerative diseases. Protein homeostasis imbalance caused by proteotoxic stress or mutant proteins leads to protein aggregation, interfering with cellular processes and promoting the progression of age-related neurodegenerative diseases. Cells have evolved mechanisms to rescue or eliminate protein aggregates, and potential therapeutic strategies targeting protein aggregates are being explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ananth R. Srinivasan, Tracy T. Tran, Nancy M. Bonini
Summary: This study investigates the role of miR-34 in age-related phenotypes in the brain, finding that miR-34 regulates translation, protein aggregation, and autophagy. Additionally, the study suggests that miR-34 may target Lst8 and other genes involved in maintaining proteostasis and brain health.
Review
Biochemistry & Molecular Biology
Diego Ruano
Summary: Aging is a progressive and irreversible biological process that leads to an increase in morbidity due to physiological deterioration. As the global population ages, the incidence of age-related pathologies such as cancer, diabetes, and neurodegenerative disorders is expected to rise. Cellular proteostasis disruption is a key feature of normal aging and is believed to play a role in the development of age-related diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Neurosciences
Arunabh Sarkar, Aamir Nazir
Summary: Maintaining cellular homeostasis through efficient clearance of aberrant proteins is crucial for health. Aging poses challenges to the proteostasis network, impacting fundamental physiological processes. Strategies to improve proteostasis may help delay age-related pathological consequences and promote healthy aging.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Cell Biology
Caterina Miceli, Manuela Leri, Massimo Stefani, Monica Bucciantini
Summary: Autophagy plays a key role in maintaining cellular and organismal homeostasis, providing the necessary energy during critical development stages and nutrient shortage. It is considered a pro-survival mechanism, but its dysregulation is associated with non-apoptotic cell death. Declining autophagy efficiency with age contributes to various pathological conditions, including cancer, cardiomyopathy, diabetes, liver disease, autoimmune diseases, infections, and neurodegeneration. Understanding the relationship between autophagy and age-related pathologies is crucial for proposing disease prevention strategies and potential clinical applications for long-term health promotion.
AGEING RESEARCH REVIEWS
(2023)
Correction
Biochemistry & Molecular Biology
Franziska Ottens, Andre Franz, Thorsteny Hoppe
Summary: A correction to the paper has been published.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Cell Biology
Jingfang Zhai, William H. H. Kongsberg, Yinbo Pan, Canhua Hao, Xiaojing Wang, Jie Sun
Summary: Research suggests that dietary changes can alter epigenetic marks associated with aging. Caloric restriction (CR) is considered an intervention to regulate aging and prolong lifespan, and has made some progress by regulating aging-related signaling pathways and intercellular signaling molecules. This review focuses on autophagy and epigenetic modifications to elaborate the molecular mechanisms by which CR delays aging by triggering autophagy, epigenetic modifications, and their interaction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yoshihisa Watanabe, Katsutoshi Taguchi, Masaki Tanaka
Summary: The HSF1-mediated stress response pathway and autophagy processes are crucial for proteostasis maintenance, longevity, and the treatment of aging-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Ankur Rakesh Dubey, Som Mohanlal Patwa, Sumit Kinger, Yuvraj Anandrao Jagtap, Prashant Kumar, Sarika Singh, Rohan Dhiman, Hem Chandra Jha, Amit Mishra
Summary: Cells synthesize new proteins after multiple molecular decisions, and protein quality control (PQC) can cope against proteostasis imbalance through alternative strategies. Accumulation of misfolded proteins is linked with neurodegenerative disorders, and understanding the abnormalities of PQC coupled molecules can help in finding biomarkers associated with neuronal disorders.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Garbin Yu, Seogang Hyun
Summary: As cells age, their ability to fold proteins properly declines, leading to the accumulation of abnormal protein aggregates and accelerating aging and neurodegenerative diseases. Mechanisms like chaperones, autophagy, and ubiquitin-proteasome system can prevent the harmful effects of abnormal proteins. Manipulating proteostasis can influence lifespan, neurotoxicity, and delay neurodegeneration, suggesting it as a potential pharmacological target for age-related diseases.
Review
Neurosciences
Sholto de Wet, Rensu Theart, Ben Loos
Summary: Neurodegenerative diseases are characterized by hydrophobic inclusion bodies that may cause neurotoxicity. Protein aggregates that appear in these diseases interrupt physiological cellular processes such as lysosomal and mitochondrial dysfunction. The review examines the autophagy signaling pathway and its connection with the mitochondrial and lysosomal system, emphasizing the importance of reducing proteins associated with neurodegenerative pathology.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Review
Geriatrics & Gerontology
Lea Danics, Anna Anoir Abbas, Balazs Kis, Karolina Pircs
Summary: As society ages, research on geroscience and healthy aging becomes more urgent. Autophagy, a cellular clearance process, plays a crucial role in determining lifespan and health. Interventions that induce autophagy have shown significant lifespan extension in experimental models. However, autophagy in humans is more complex and clinical trials targeting autophagy have mixed results. The use of more human-relevant preclinical models could improve drug efficacy and clinical trial outcomes.
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Cell Biology
Yi Liu, Lan Tan, Meng-Shan Tan
Summary: Chaperone-mediated autophagy (CMA) is a crucial process for the selective degradation of intracellular components, particularly aggregate-prone proteins, and is involved in the maintenance of proteostasis. Disruption of proteostasis, which refers to the balance between protein synthesis and degradation, can lead to neurodegenerative diseases, especially in postmitotic neurons. Studies have shown that CMA dysfunction, similar to aging, plays a role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease. This review focuses on the molecular mechanisms, physiological function, and regulation of CMA, as well as its potential role and recent advances in the therapy of neurodegenerative diseases.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Satyendra Singh, Ketan Kumar, Mamta Panda, Aryan Srivastava, Amit Mishra, Vijay Kumar Prajapati
Summary: Immunotherapy using immune checkpoint inhibitors has been widely used for treating various cancers. This study screened small-molecule inhibitors against immune cell checkpoints and identified three potential inhibitors that showed the ability to activate human immune cells and control the spread of diseases. Further validation is needed for development as immunotherapeutics.
MOLECULAR DIVERSITY
(2023)
Review
Pharmacology & Pharmacy
Anjali Sangeeth, Mahesh Malleswarapu, Amit Mishra, Ravi Kumar Gutti
Summary: Long non-coding RNAs (lncRNAs) are more than 200 bases long and have major regulatory roles in biologic processes such as hematopoeisis and metabolism. Recent studies have shown that lncRNAs directly and indirectly participate in metabolism by regulating enzymes and signaling pathways, and can alter tissue-specific metabolism. Understanding the different functions of lncRNAs in cellular pathways helps in predicting and treating myelodysplastic and metabolic disorders. This review provides an overview of ncRNAs, their effects on hematopoiesis and metabolism, and discusses their prognostic value and potential as biomarkers and therapeutic targets.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2023)
Review
Oncology
Ayeman Amanullah, Arun Upadhyay, Rohan Dhiman, Sarika Singh, Amit Kumar, Dinesh Kumar Ahirwar, Ravi Kumar Gutti, Amit Mishra
Summary: This article summarizes the current understanding of the drug diclofenac, discussing its potential applications and existing limitations. It highlights the need for further research to better understand its mechanisms and improve its effectiveness against various diseases.
Article
Biology
Satyendra Singh, Abhishek Rao, Ketan Kumar, Amit Mishra, Vijay Kumar Prajapati
Summary: The recent outbreak of monkeypox disease, declared a public health emergency of international outrage by the WHO, prompted the development of a highly immunogenic multiepitope subunit vaccine against the virus. Through in silico translational vaccinomics technique, antigenic B cell and T cell epitopes were predicted and conjugated with unique linkers. An adjuvant and a pan-HLA DR sequence were included to elicit a robust immune response. The stability and efficacy of the vaccine candidate were confirmed through molecular dynamics and immune dynamics simulation studies.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mamta Panda, Elora Kalita, Satyendra Singh, Ketan Kumar, Vijay Kumar Prajapati
Summary: This study utilized computational immunoinformatics methodology to investigate various anti-SARS-CoV-2 nanobodies, resulting in the development of a broad-spectrum nanobody with potent antiviral activity. By combining it with an antiviral peptide, a multispecific nanobody-peptide conjugate was created to effectively block viral entry. This research provides a promising therapeutic tool for combating viral entry during COVID-19 illness.
MOLECULAR DIVERSITY
(2023)
Article
Chemistry, Multidisciplinary
Satyendra Singh, Priya Chauhan, Vinita Sharma, Abhishek Rao, Bajarang Vasant Kumbhar, Vijay Kumar Prajapati
Summary: In this study, structure-based virtual screening was used to identify potential peptides targeting SARS-CoV-2. Two promising peptides were selected and evaluated through 3D modeling, docking analysis, and molecular dynamics simulations, suggesting their potential as broad-spectrum drugs for treating SARS-CoV-2.
STRUCTURAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Rupal Ojha, Satyendra Singh, Nidhi Gupta, Ketan Kumar, Aditya K. Padhi, Vijay Kumar Prajapati
Summary: In this study, a multi-pathogen vaccine was designed by incorporating immunogenic proteins from S. pneumoniae, H. influenzae, and M. tuberculosis, which are dominantly associated with SARS-CoV-2. The selected epitopes were predicted and linked with adjuvant and linkers to enhance immunogenicity, stability, and flexibility. Docking and MD simulation study revealed efficient binding of the chimeric vaccine with the TLR4 receptor. The three-dose injection of this vaccine showed high levels of cytokines and IgG, suggesting its potential to decrease disease severity and prevent the pandemic.
BIOTECHNOLOGY LETTERS
(2023)
Article
Medicine, Research & Experimental
Rupal Ojha, Kailash Chand, Balachandar Vellingiri, Vijay Kumar Prajapati
Summary: This study aimed to develop a more potent vaccine candidate against visceral leishmaniasis. The recombinant vaccine candidate Leisp was expressed, purified, and characterized. The expression modulation of different cytokines was evaluated in THP1 cell lines, and the impact of the vaccine protein candidates in infected conditions was determined. The results showed that the candidate vaccine could significantly upregulate the expression of pro-inflammatory cytokines, indicating its potential inductive efficacy for inflammation in Leishmania-infected cells.
Review
Cell Biology
Sumit Kinger, Ankur Rakesh Dubey, Prashant Kumar, Yuvraj Anandrao Jagtap, Akash Choudhary, Amit Kumar, Vijay Kumar Prajapati, Rohan Dhiman, Amit Mishra
Summary: Amyotrophic lateral sclerosis (ALS) is a neuronal degenerative condition caused by the accumulation of mutant aberrantly folded proteins. The involvement of chaperones in ALS pathogenesis is not thoroughly understood, making it challenging to develop effective treatments for the disease.
Review
Biochemistry & Molecular Biology
Annu Rani, Vaishali Saini, Priyanka Patra, Tanish Prashar, Rajan Kumar Pandey, Amit Mishra, Hem Chandra Jha
Summary: EGCG, a polyphenolic compound in green tea, has pleiotropic bioactivities to combat neurological diseases and inhibit the effects of neurotropic viruses.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Neurosciences
Arun Upadhyay, Deepak Chhangani, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savas
Summary: This study investigated the formation and neurotoxicity of amyloid fibrils in Alzheimer's disease (AD). By isolating pure amyloid fibrils, the composition of the fibrils was identified and 20 new amyloid-associated proteins were discovered. The findings suggest that fibril-associated proteins play a key role in amyloid formation and AD pathology.
MOLECULAR NEURODEGENERATION
(2023)
Review
Environmental Sciences
Mahalaxmi Iyer, Uttpal Anand, Saranya Thiruvenkataswamy, Harysh Winster Suresh Babu, Arul Narayanasamy, Vijay Kumar Prajapati, Chandan Kumar Tiwari, Abilash Valsala Gopalakrishnan, Elza Bontempi, Christian Sonne, Damia Barcelo, Balachandar Vellingiri
Summary: Carcinogenic metals, particularly chromium (Cr) and its derivatives, including Cr(VI)-induced, have widespread distribution and pose a concern for adverse environmental and human health effects. They cause DNA epigenetic alterations resulting in heritable changes in gene expression. This review discusses the role of Cr(VI) in epigenetic changes, highlighting the importance of considering epigenetics as a possible mechanism underlying Cr(VI) toxicity and cell-transforming ability. More clinical and preventative measures are needed to better understand the toxicity and safeguard employees against cancer.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Biochemistry & Molecular Biology
Uttpal Anand, Abhijit Dey, Arvind K. Singh Chandel, Rupa Sanyal, Amarnath Mishra, Devendra Kumar Pandey, Valentina De Falco, Arun Upadhyay, Ramesh Kandimalla, Anupama Chaudhary, Jaspreet Kaur Dhanjal, Saikat Dewanjee, Jayalakshmi Vallamkondu, Jose M. Perez de la Lastra
Summary: Cancer is a deadly disease characterized by uncontrolled cell proliferation and the formation of aggressive malignancies. In recent years, advances in molecular understanding have led to the development of new therapeutic approaches such as targeted drug therapy and immunotherapy. Despite the potential of these treatments, chemotherapy remains a commonly used option due to its effectiveness in advanced-stage malignancies. This report reviews the current advancements in chemotherapy and discusses the emerging role of targeted therapies in improving clinical outcomes for cancer patients.
Article
Chemistry, Physical
Satyendra Singh, Abhishek Rao, Anshuman Mishra, Amit Mishra, Vijay Kumar Prajapati
Summary: Following a multi-country outbreak, the monkeypox (MPX) disease was declared a global public health emergency on July 23, 2022. Current treatment options for MPX involve antiviral medications designed for smallpox virus and lack specific therapies with minimal side effects. This study used the Thera-SAbDab database to screen monoclonal antibodies (mAbs) against critical enzymes of the monkeypox virus and identified eculizumab and vofatamab as potential inhibitors. Mutant mAbs were also generated and evaluated for their binding affinity. These findings hold promise for future in vitro and in vivo experiments to neutralize the MPX virus in infected patients.
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2023)