Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects

Biotechnological Applications of MBD Domain Proteins for DNA Methylation Analysis

(2019) Albert Jeltsch et al.   JOURNAL OF MOLECULAR BIOLOGY

A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs

(2018) Christian Pflueger et al.   GENOME RESEARCH

Structural basis for DNMT3A-mediated de novo DNA methylation

(2018) Zhi-Min Zhang et al.   NATURE

The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update

(2018) Enis Afgan et al.   NUCLEIC ACIDS RESEARCH

Targeted DNA demethylation of theArabidopsisgenome using the human TET1 catalytic domain

(2018) Javier Gallego-Bartolomé et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-wide tracking of dCas9-methyltransferase footprints

(2018) Christina Galonska et al.   Nature Communications

Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System

(2018) Nina Xie et al.   Stem Cells International

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

(2018) Lin Lin et al.   GigaScience

Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria

(2018) Tina Xiong et al.   PLoS One

Editing DNA Methylation in the Mammalian Genome

(2016) X. Shawn Liu et al.   CELL

Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions

(2016) Sumiyo Morita et al.   NATURE BIOTECHNOLOGY

Loss-of-function genetic tools for animal models: cross-species and cross-platform differences

(2016) Benjamin E. Housden et al.   NATURE REVIEWS GENETICS

Allosteric control of mammalian DNA methyltransferases – a new regulatory paradigm

(2016) Albert Jeltsch et al.   NUCLEIC ACIDS RESEARCH

deepTools2: a next generation web server for deep-sequencing data analysis

(2016) Fidel Ramírez et al.   NUCLEIC ACIDS RESEARCH

Efficient targeted DNA methylation with chimeric dCas9–Dnmt3a–Dnmt3L methyltransferase

(2016) Peter Stepper et al.   NUCLEIC ACIDS RESEARCH

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

Epigenome Editing: State of the Art, Concepts, and Perspectives

(2016) Goran Kungulovski et al.   TRENDS IN GENETICS

Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation

(2016) James I. McDonald et al.   Biology Open

TALE-mediated epigenetic suppression of CDKN2A increases replication in human fibroblasts

(2015) Diana L. Bernstein et al.   JOURNAL OF CLINICAL INVESTIGATION

Stable oncogenic silencing in vivo by programmable and targeted de novo DNA methylation in breast cancer

(2015) S Stolzenburg et al.   ONCOGENE

Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained

(2015) Goran Kungulovski et al.   Epigenetics & Chromatin

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

(2014) Alexandra E. Briner et al.   MOLECULAR CELL

Targeted Methylation of the Epithelial Cell Adhesion Molecule (EpCAM) Promoter to Silence Its Expression in Ovarian Cancer Cells

(2014) Suneetha Nunna et al.   PLoS One

Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53

(2014) Ingrid E. B. Lawhorn et al.   PLoS One

PEAR: a fast and accurate Illumina Paired-End reAd mergeR

(2013) J. Zhang et al.   BIOINFORMATICS

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Epigenetic reprogramming of cancer cells via targeted DNA methylation

(2012) Ashley G. Rivenbark et al.   Epigenetics

Targeted Methylation and Gene Silencing of VEGF-A in Human Cells by Using a Designed Dnmt3a–Dnmt3L Single-Chain Fusion Protein with Increased DNA Methylation Activity

(2012) Abu Nasar Siddique et al.   JOURNAL OF MOLECULAR BIOLOGY

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CpG islands and the regulation of transcription

(2011) A. M. Deaton et al.   GENES & DEVELOPMENT

Function and disruption of DNA Methyltransferase 3a cooperative DNA binding and nucleoprotein filament formation

(2011) Arumugam Rajavelu et al.   NUCLEIC ACIDS RESEARCH

Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L

(2008) Renata Z. Jurkowska et al.   NUCLEIC ACIDS RESEARCH