Article
Chemistry, Physical
Lucas W. Henderson, Edie M. Sharon, Amit K. S. Gautam, Adam J. Anthony, Martin F. Jarrold, David H. Russell, Andreas Matouschek, David E. Clemmer
Summary: Mass spectrometry studies show that the stability of S. cerevisiae 20S proteasome undergoes related configurations and transitions during temperature changes, possibly linked to the opening of the proteolytic core. The study indicates that the proteasome remains intact and all transitions are reversible. Three types of structures are identified based on thermodynamic analysis: energetically stabilized closed configurations, high-entropy precursor states, and open pore structures. Opening of the 20S pore in the absence of the regulatory unit involves a charge-priming process. However, only a small fraction of precursor configurations actually open to expose the catalytic cavity.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Review
Biochemistry & Molecular Biology
Daniel Betancourt, Tomiwa Lawal, Robert J. Tomko Jr
Summary: The 26S proteasome is a large and complex protease that is implicated in numerous human diseases. The assembly of the proteasome requires the coordinated actions of intrinsic proteasome subunits and dedicated assembly chaperones. Recent advances in cryo-electron microscopy have revealed the dynamic nature of the proteasome, with conformational changes occurring during ligand binding and the proteolytic cycle. This review focuses on proteasome assembly, particularly on how conformational dynamics guide biogenesis events, and highlights key questions in this field.
Review
Plant Sciences
Dania P. Lobaina, Roberto Tarazi, Tamara Castorino, Maite F. S. Vaslin
Summary: The ubiquitin-proteasome system (UPS) is crucial for maintaining cellular balance and plays a key role in DNA repair, gene transcription, and protein activation. Viruses often manipulate the UPS to maintain viral protein levels and evade host defenses. Understanding the mechanisms used by plant viruses to subvert host defenses is important for developing biotechnological approaches for viral resistance.
Review
Cell Biology
Xiu Yin, Qingbin Liu, Fen Liu, Xinchen Tian, Tinghao Yan, Jie Han, Shulong Jiang
Summary: This review summarizes the role of ubiquitination in tumorigenesis and related signaling pathways, aiming to provide a reference for future exploration of ubiquitination and potential targets for cancer therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shion Osana, Yasuo Kitajima, Suzuki Naoki, Hiroaki Takada, Kazutaka Murayama, Yutaka Kano, Ryoichi Nagatomi
Summary: The proteasome and aminopeptidases are thought to be involved in protein synthesis through intracellular energy production by recycled-amino acid metabolism, thereby maintaining myoblast integrity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Gregor Habeck, Joerg Schweiggert
Summary: Cellular processes rely heavily on dynamic proteomes, with ubiquitylation playing a key role in triggering transitions between different cellular states. While ubiquitin-related events in ciliogenesis are usually thought to be initiated just before their effects are evident, evidence suggests that some events may actually begin earlier in the cell cycle.
Article
Multidisciplinary Sciences
Donghoon Lee, Alfred L. Goldberg
Summary: Heat shock induces protein unfolding, leading to increased activity of proteasomes and degradation of ubiquitinated proteins. This response helps cells clear damaged proteins and maintain cellular homeostasis under proteotoxic stress conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Chunmei Gong, Laura Bonfili, Yadong Zheng, Valentina Cecarini, Massimiliano Cuccioloni, Mauro Angeletti, Giulia Dematteis, Laura Tapella, Armando A. Genazzani, Dmitry Lim, Anna Maria Eleuteri
Summary: Alzheimer's disease (AD) is characterized by dysfunctions in the ubiquitin-proteasome system (UPS) and autophagy. In this study, researchers obtained immortalized astrocytes from the hippocampus of AD mice and wild-type mice to investigate the proteolytic systems involved. They found downregulation of proteasomal activities and increased autophagy activation in the AD astrocytes. Treatment with 4-phenylbutyric acid partially restored the proteolysis processes. These findings provide further validation of the AD in vitro model and suggest a new mechanism of action for 4-phenylbutyric acid in neurodegenerative disorders.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hyesu Lee, Sungwook Kim, Daeyoup Lee
Summary: The 26S proteasome, consisting of a 20S core particle and a 19S regulatory particle, plays a critical role in regulating gene expression and silencing through its proteolytic and non-proteolytic functions. It modulates gene expression in euchromatin by modifying histones, activating transcription, initiating and terminating transcription, mRNA export, and maintaining transcriptome integrity. In gene silencing, it influences heterochromatin formation, spreading, and subtelomere silencing by degrading specific proteins and interacting with anti-silencing factors.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Biochemistry & Molecular Biology
Catherine Kim, Meredith Juncker, Ryan Reed, Arthur Haas, Jessie Guidry, Michael Matunis, Wei-Chih Yang, Joshua Schwartzenburg, Shyamal Desai
Summary: In cells treated with mitochondrial stressors, SUMOylation of Mfn1/2 facilitates the aggregation of damaged mitochondria at the perinuclear region, potentially through acting as a molecular glue to interact with other proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Indrajit Sahu, Monika Bajorek, Xiaolin Tan, Madabhushi Srividya, Daria Krutauz, Noa Reis, Pawel A. Osmulski, Maria E. Gaczynska, Michael H. Glickman
Summary: The active sites of the 26S proteasome are located in the catalytic chamber of its 20S core particle. Access to this chamber is through a narrow channel formed by the seven outer alpha subunits. The gate blocking this channel is formed by the N-termini of neighboring alpha subunits, and attachment of activators rearranges these N-termini, allowing entry of substrates. A hydrogen bond between an invariant tyrosine (Y) in each subunit and a conserved aspartate (D) in its counterclockwise neighbor stabilizes the open state, except for the alpha 1-alpha 2 pair which leaves a gap in the ring. The third residue (X) of the YD(X) motif aligns with the open channel, and deletion of the alpha 2 N-terminal tail attenuates proteolysis despite the appearance of an open gate state.
Article
Biotechnology & Applied Microbiology
Jianqing Chen, Jinyu Zhou, Yanhong Hong, Zekun Li, Xiangyu Cheng, Aiying Zheng, Yilin Zhang, Juanjuan Song, Guifeng Xie, Changmei Chen, Meng Yuan, Tengyun Wang, Qingxi Chen
Summary: The study identified novel candidate RGs with greater stability in transcriptome and RT-qPCR analyses, with most stably expressed genes associated with the ubiquitin proteasome system. RPT6A and RPN5A were recommended as the optimal combination for normalization of gene expression during strawberry receptacle development.
Article
Cell Biology
Jeremy J. Work, Onn Brandman
Summary: This study reveals the distinct effects of proteotoxic stressors on the proteome and UPS regulation, as well as the versatility of cells in adapting the UPS under different stressors.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Plant Sciences
Yi Sun, R. Paul Jarvis
Summary: Chloroplasts are plant organelles responsible for photosynthesis and other vital functions, and their complex proteome is controlled by diverse processes of protein translocation and degradation. Most chloroplast proteins are imported from the cytosol and then sorted to different organellar subcompartments. Chloroplast proteolysis involves proteases of prokaryotic origin and the nucleocytosolic ubiquitin-proteasome system, which regulate protein import and reconfigure chloroplast functions in response to developmental and environmental signals.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Philip Coffino, Yifan Cheng
Summary: Allostery-regulation at distant sites is a crucial concept in biology, and the proteasome provides multiple examples of this regulation. It can span a long distance, influencing the interactions and assembly of proteasome modules, and explain how diverse populations of proteasome assemblies are built and maintained in cells.
Article
Cell Biology
Philippe A. Bilodeau, Erin S. Coyne, Simon S. Wing
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2016)
Article
Medicine, Research & Experimental
Derek T. Hall, Takla Griss, Jennifer F. Ma, Brenda Janice Sanchez, Jason Sadek, Anne Marie K. Tremblay, Souad Mubaid, Amr Omer, Rebecca J. Ford, Nathalie Bedard, Arnim Pause, Simon S. Wing, Sergio Di Marco, Gregory R. Steinberg, Russell G. Jones, Imed-Eddine Gallouzi
EMBO MOLECULAR MEDICINE
(2018)
Article
Multidisciplinary Sciences
Ken-ichiro Tanaka, Yingben Xue, Loan Nguyen-Yamamoto, John A. Morris, Ippei Kanazawa, Toshitsugu Sugimoto, Simon S. Wing, J. Brent Richards, David Goltzman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Endocrinology & Metabolism
Olasunkanmi A. J. Adegoke, Stephanie Chevalier, Jose A. Morais, Rejeanne Gougeon, Scot R. Kimball, Leonard S. Jefferson, Simon S. Wing, Errol B. Marliss
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2009)
Article
Biochemistry & Molecular Biology
Thomas M. Kitzler, Joan Papillon, Julie Guillemette, Simon S. Wing, Andrey V. Cybulsky
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2012)
Article
Reproductive Biology
Nathalie Bedard, Yaoming Yang, Mary Gregory, Daniel G. Cyr, Joao Suzuki, Xiaomin Yu, Ri-Cheng Chian, Louis Hermo, Cristian O'Flaherty, Charles E. Smith, Hugh J. Clarke, Simon S. Wing
BIOLOGY OF REPRODUCTION
(2011)
Article
Oncology
Francis Robert, John R. Mills, Aouod Agenor, Dantong Wang, Sergio DiMarco, Regina Cencic, Michel L. Tremblay, Imed Eddine Gallouzi, Siegfried Hekimi, Simon S. Wing, Jerry Pelletier
Review
Medical Laboratory Technology
Simon S. Wing, Stewart H. Lecker, R. Thomas Jagoe
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2011)
Review
Biochemistry & Molecular Biology
Simon S. Wing
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Thomas M. Durcan, Maria Kontogiannea, Nathalie Bedard, Simon S. Wing, Edward A. Fon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Yu Lu, Nathalie Bedard, Simone Chevalier, Simon S. Wing
Article
Multidisciplinary Sciences
Rohini Bose, Kai Sheng, Adel R. Moawad, Gurpreet Manku, Cristian O'Flaherty, Teruko Taketo, Martine Culty, Kin Lam Fok, Simon S. Wing
SCIENTIFIC REPORTS
(2017)
Article
Endocrinology & Metabolism
Erin S. Coyne, Nathalie Bedard, Ying Jia Gong, May Faraj, Andre Tchernof, Simon S. Wing
Article
Geriatrics & Gerontology
Anne-Julie Tessier, Simon S. Wing, Elham Rahme, Jose A. Morais, Stephanie Chevalier
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2019)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)