Article
Agriculture, Multidisciplinary
Shaoqi Li, Ghizlane Tahiri, Junhuan Yang, Hassan Mohamed, Qing Liu, Wenyue Shi, Sergio Lopez-Garcia, Victoriano Garre, Yuanda Song
Summary: Lipid accumulation in oleaginous organisms is regulated by AMP deaminase (AMPD), which controls intracellular AMP concentration. In this study, we investigated the role of AMPD in lipogenesis in the oleaginous fungus Mucor circinelloides. We found that deletion of ampd1 and ampd2 increased lipid contents under nitrogen-limited conditions. Unexpectedly, overexpression of ampd genes affected nitrogen consumption and induced lipid accumulation even under nitrogen-rich conditions. These findings highlight the importance of AMPD in lipid synthesis and its impact on nitrogen metabolism and energy balance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Junhuan Yang, Jose T. Canovas-Marquez, Pengcheng Li, Shaoqi Li, Junchao Niu, Xiuwen Wang, Yusuf Nazir, Sergio Lopez-Garcia, Victoriano Garre, Yuanda Song
Summary: In this study, the deletion of two plasma membrane malate transporters in Mucor circinelloides WJ11 led to increased lipid content and altered expression of key genes involved in lipid biosynthesis, providing evidence of the association between plasma membrane malate transporters and lipid accumulation in oleaginous fungi.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Multidisciplinary
M. N. A. M. Yusoff, N. W. M. Zulkifli, N. L. Sukiman, M. A. Kalam, H. H. Masjuki, A. Z. Syahir, M. S. N. Awang, M. A. Mujtaba, J. Milano, A. H. Shamsuddin
Summary: This study aimed to develop biodiesel using a ternary oil mixture consisting of waste cooking oil, Jatropha curcas oil, and palm oil. The parameters affecting biodiesel yield were optimized through response surface methodology. The results showed that the synergistic mixture of ternary oil exhibited improved cold flow properties, oxidation stability, and cetane number.
ALEXANDRIA ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Xinyi Zan, Jianing Sun, Linfang Chu, Fengjie Cui, Shuhao Huo, Yuanda Song, Mattheos A. G. Koffas
Summary: Overexpressing xylose isomerase gene xylA or xylulokinase gene xks1 can significantly increase xylose utilization and enhance lipid content in the co-fermentation of glucose and xylose. The overexpression of xylA and/or xks1 is an effective strategy to improve the co-utilization of xylose and glucose, alleviate glucose repression, and produce lipids from lignocellulosic biomass in the oleaginous fungus M. circinelloides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Shaoqi Li, Junhuan Yang, Hassan Mohamed, Xiuwen Wang, Shuxian Pang, Chen Wu, Sergio Lopez-Garcia, Yuanda Song
Summary: The study discovered the important role of ADA in lipid biosynthesis and nitrogen metabolism. Knockout of the ada gene increased the lipid content of the fungus, while overexpression of ada gene led to increased lipid accumulation at the early growth stage. Transcriptional analysis revealed that deletion of ada activated key genes involved in lipid biosynthesis and suppressed genes related to lipolysis, while overexpression of ada showed the opposite effect.
Article
Microbiology
Abu Bakr Ahmad Fazili, Aabid Manzoor Shah, Tahira Naz, Shaista Nosheen, Wu Yang, Victoriano Garre, Younis Majeed, Mohammed Khalid Al-Sadoon, Yuanda Song
Summary: This study evaluated the role of two cytosolic ME genes in lipid accumulation of M. circinelloides and found that overexpressing these genes can significantly increase lipid synthesis and overall GLA content.
Article
Biotechnology & Applied Microbiology
Feifei Xin, Wenrui Dang, Yufei Chang, Ruixue Wang, Hongjuan Yuan, Zhike Xie, Chuanchuan Zhang, Sha Li, Hassan Mohamed, Huaiyuan Zhang, Yuanda Song
Summary: During solid-state fermentation, the oleaginous fungus Mucor circinelloides showed higher fatty acid content in mycelia compared to spores, although mycelia had lower biomass. Transcriptomic analysis revealed differential gene expression between spores and mycelia, including up-regulation of triglyceride-related synthases, ATP: citrate lyase, and leucine and isoleucine metabolism in mycelia. Autophagy pathway was also up-regulated in mycelia, suggesting its involvement in lipid accumulation in M. circinelloides.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Yao Zhang, Yueping Yang, Qing Liu, Shaoqi Li, Yuanda Song
Summary: In this study, the effects of Snf-ss subunit on the growth and lipid accumulation of M. circinelloides were analyzed and compared under different carbon nutrient signals. The results showed that the knockout of Snf-ss subunit resulted in significantly increased lipid accumulation, especially in response to xylose or mixed glucose and xylose. Furthermore, the transcriptional level of ACC1 was also affected by the deletion of Snf-ss subunit. This study provides insights into the regulation of lipid production in M. circinelloides and its potential application in lignocellulosic lipid production.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yao Zhang, Yuanda Song
Summary: By overexpressing the genes coding for xylose isomerase and xylulokinase in Mucor circinelloides, this study successfully increased fatty acid contents and xylose uptake rates, leading to enhanced lipid synthesis. Cultivating the engineered strains in corn straw hydrolysate medium resulted in promising biomass and lipid yields.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Caili Sun, Aabid Manzoor Shah, Junhuan Yang, Zongmin Wang, Lanlan Zhu, Yuanda Song
Summary: Mucor circinelloides is an oleaginous fungus capable of utilizing various carbon sources, with exogenous plant oil leading to increased lipid biosynthesis. The study highlights the significant increase in lipid content when using exogenous plant oil as a carbon source, along with the upregulation of genes and pathways related to lipid synthesis.
NATURAL PRODUCT COMMUNICATIONS
(2021)
Article
Microbiology
Aabid Manzoor Shah, Hassan Mohamed, Abu Bakr Ahmad Fazili, Wu Yang, Yuanda Song
Summary: In this study, the alcohol dehydrogenase gene (ADH1) of Mucor circinelloides WJ11 was deleted, resulting in a significant reduction in ethanol concentration and a decrease in lipid and fatty acid content. The growth of the mutant strain showed minimal change. However, when external ethanol was added, the lipid and fatty acid content of the mutant strain were restored. This study provides the first evidence linking alcohol dehydrogenase to lipid production in M. circinelloides.
Article
Microbiology
Xiuwen Wang, Hassan Mohamed, Yonghong Bao, Chen Wu, Wenyue Shi, Yuanda Song, Junhuan Yang
Summary: This study successfully increased lipid accumulation in low lipid-producing fungi through metabolic engineering technology. The results showed that overexpression of malate transporter (mt) and 2-oxoglutarate: malate antiporter (sodit) genes can significantly enhance lipid content.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Hakun Wirawasista Aparamarta, Setiyo Gunawan, Surya Iryana Ihsanpuro, Imam Safawi, Donny Satria Bhuana, Aang Firmansyah Mochtar, M. Yusril Izhar Noer
Summary: Nyamplung oil is a promising raw material for biodiesel production, especially using the MAE method which is more efficient and energy-saving.
Article
Biochemistry & Molecular Biology
Jose Pinela, Beatriz de la Fuente, Matilde Rodrigues, Tania C. S. P. Pires, Filipa Mandim, Andre Almeida, Maria Ines Dias, Cristina Caleja, Lillian Barros
Summary: This study aims to explore the potential of microwave-assisted extraction (MAE) technique for obtaining high-quality oil from fish by-products. The optimal MAE conditions allow for the recovery of 60 to 100% of oil in a short time with less solvent consumption. The oils obtained through MAE showed antimicrobial and anti-inflammatory effects, making them a promising ingredient for pet food and other products.
Article
Biotechnology & Applied Microbiology
Shaista Nosheen, Tahira Naz, Junhuan Yang, Syed Ammar Hussain, Abu Bakr Ahmad Fazili, Yusuf Nazir, Shaoqi Li, Hassan Mohamed, Wu Yang, Kiren Mustafa, Yuanda Song
Summary: Our study revealed that the Snf-beta gene is crucial in regulating lipid accumulation in M. circinelloides WJ11. By manipulating the Snf-beta gene, we can alter lipid content in the strain. Furthermore, changes in GLA content were observed in Snf-beta knockout and overexpressing strains.
MICROBIAL CELL FACTORIES
(2021)
Article
Chemistry, Physical
Rosemar de Lima, Heitor B. S. Bento, Cristiano E. R. Reis, Renata N. Vilas Boas, Larissa de Freitas, Ana K. F. Carvalho, Heizir F. de Castro
Summary: Six lipases were immobilized on a styrene-divinylbenzene support and evaluated for batch esterification of stearic acid with fusel oil. After screening, the two most suitable lipases achieved high conversion rates and productivities, showing potential as substitutes for fossil-derived lubricants.
Review
Biotechnology & Applied Microbiology
Renata N. Vilas Boas, Heizir F. de Castro
Summary: The esterification reactions catalyzed by lipases are important biochemical processes in industry, allowing for the production of high-quality products with high yield. This review highlights the increasing attention towards enzyme-catalyzed esterification, specifically lipase-catalyzed reactions, due to the growing use of organic esters in the chemical and biotechnology industry. The advantages of biotechnological processes, including high catalytic efficiency, mild operating conditions, and selectivity of natural catalysts, make them a competitive alternative to chemical methods. Factors affecting lipase-catalyzed esterification reactions, such as the source of lipase, form of biocatalyst, polarity of the reaction medium, and alcohol-to-acid molar ratio, are also discussed.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Physical
Heitor B. S. Bento, Cristiano E. R. Reis, Pedro A. Pinto, Daniela V. Cortez, Renata N. Vilas Boas, Tales A. Costa-Silva, Ana K. F. Carvalho, Heizir F. de Castro
Summary: This study focused on immobilizing commercial lipase onto magnetic particles for the synthesis of biodiesel, showing improved thermal stability and esterification activity. The immobilized system demonstrated high productivity and met the quality requirements of biodiesel fuel standards under continuous operation.
Article
Energy & Fuels
Renata N. Vilas Bo, Rosemar de Lima, Adriano A. Mendes, Larissa Freitas, Heitor B. S. Bento, Ana Karine F. de Carvalho, Heizir F. de Castro
Summary: The study explored the synthesis of biolubricants using different sources of lipase immobilized on a specific resin, with Rhizopus oryzae lipase immobilized on poly(styrene/divinylbenzene) copolymer resin showing the best performance. The optimal conditions included a 1:1.5 ratio of isoamyl alcohol to oleic acid and a reaction temperature of 45 degrees C.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2021)
Article
Energy & Fuels
Savienne M. F. E. Zorn, Ana Paula T. da Silva, Eduardo H. Bredda, Heitor B. S. Bento, Guilherme A. Pedro, Ana Karine F. Carvalho, Messias Borges Silva, Patricia C. M. Da Ros
Summary: This study successfully obtained biolubricants through transesterification reactions of microbial biomass, opening up new possibilities for producing environmentally friendly lubricants. The fatty acid profiles of the obtained oils were analyzed, and the optimal configuration for improving oxidation resistance was determined. A catalyst with high transesterification yields was used, showing promise for producing biolubricants from microbial biomass.
Article
Engineering, Environmental
Tarsila F. de Castro, Daniela Cortez, Daniel B. Goncalves, Heitor B. S. Bento, Rhyan L. N. Goncalves, Tales A. Costa-Silva, Bruno C. Gambarato, Heizir F. de Castro, Ana Karine F. de Carvalho
Summary: This study investigates the direct use of Penicillium cells as biocatalysts for hydrolyzing vegetable oils under low-power ultrasonic irradiation. The results show that Penicillium cells with specific lipase can efficiently hydrolyze vegetable oils high in fatty acids, and ultrasound waves can improve the reaction efficiency. However, the presence of fatty acids with 18 carbons in the substrate negatively affects the hydrolysis.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2022)
Article
Energy & Fuels
Sara A. Machado, Cristiano E. Rodrigues Reis, Heitor B. S. Bento, Ana K. F. Carvalho, Tales A. Costa-Silva, Leyvison Rafael da Conceicao, Domingos S. Giordani, Heizir F. de Castro
Summary: The feasibility of using a Keggin-structured heteropolyacid, H3PW12O40 (HPW), supported onto Nb2O5 as a catalyst for the esterification of free fatty acids with ethanol was demonstrated. The catalyst showed high conversions and met the regulatory limits for biodiesel. The use of heterogeneous acid catalysts, such as HPW/Nb2O5, presents a potentially feasible alternative for working with challenging feedstocks.
Article
Biochemistry & Molecular Biology
Savienne Zorn, Ana Carvalho, Heitor Bento, Bruno Gambarato, Guilherme Pedro, Ana da Silva, Rhyan Goncalves, Patricia Da Ros, Messias Silva
Summary: In this study, a lichen-like structure was formed by the combination of fungal mycelium and algal cells, resulting in high efficiency of cell recovery and biomass accumulation. The structure also showed promising lipid accumulation for industrial applications.
Article
Energy & Fuels
Juan Daniel Rivaldi, Hyun Ho Shin, Federico Colman, Carlos Sauer, Javier Gonzalez, Orlando Rojas, Mario Smidt, Edelira Velazquez, Karen Martinez
Summary: This study provides an overview of available residual biomass from agricultural fields and agro-industries in Paraguay and presents the thermochemical profiles of these biomass samples. The results suggest that the residual biomass can be used as solid fuel and has the potential to generate a significant amount of thermal and electrical energy.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Green & Sustainable Science & Technology
Maria Edelira Velazquez, Omayra Beatriz Ferreiro, Diego Batista Menezes, Yendry Corrales-Urena, Jose Roberto Vega-Baudrit, Juan Daniel Rivaldi
Summary: This study investigated the utilization of residual biomasses from agro-industries in Paraguay, such as soybean hulls and sugarcane bagasse, for nanocellulose extraction. The obtained nanocellulose exhibited good thermal stability and formed stable dispersions in water.
Article
Engineering, Environmental
Cristiano E. Rodrigues Reis, Nelson Libardi Junior, Heitor B. S. Bento, Ana Karine F. de Carvalho, Luciana Porto de Souza Vandenberghe, Carlos Ricardo Soccol, Tejraj M. Aminabhavi, Anuj K. Chandel
Summary: This review provides a comprehensive analysis of specific strategies, such as applying extraneous proteins and additives, enzyme recycling, and the effect of washing pre-treated biomass on the production of 2G sugar at large scales in lignocellulose biorefineries.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Igor G. Vidigal, Mariana P. Melo, Patricia C. M. Da Ros, Ana K. F. Carvalho, Severino M. Alencar, Ana L. G. Ferreira
Summary: This work presents a bibliometric analysis of recent articles on the applications of thermal processing in foods and their effects on physicochemical and rheological properties. The analysis reveals that China, USA, Brazil, India, and Spain are the leading countries in food thermal processing research. Traditional thermal processing continues to be studied in fruit applications, while the use of unconventional or emerging technologies is expected to bring significant advances to the food industry in the future.
Article
Chemistry, Multidisciplinary
Arthur O. Preto, Willian S. M. Reis, Ana K. F. de Carvalho, Ernandes B. Pereira
Summary: This study focused on the production of mycelium-bound lipases of Rhizopus oryzae through submerged cultivation for the synthesis of significant ester compounds in the cosmetic industry. The research found that the lipolytic biomass of Rhizopus oryzae showed good performance in synthesizing isoamyl palmitate ester with high conversion rates.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Engineering, Chemical
T. A. Costa-Silva, A. K. F. Carvalho, C. R. F. Souza, L. Freitas, H. F. De Castro, W. P. Oliveira
Summary: The overall stability of Candida rugosa lipase (CRL) was improved through immobilization onto low-cost eco-friendly carriers and drying processes. The lipase immobilized on glutaraldehyde-activated rice husk showed the best performance and retained 94.1% of the initial activity. The thermostability of all immobilized derivatives was significantly improved.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2022)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
