期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 105, 期 13, 页码 5565-5575出版社
SPRINGER
DOI: 10.1007/s00253-021-11392-x
关键词
Mucor circinelloides; Xylose isomerase; Xylulokinase; Xylose utilization; Lipid production
资金
- National Natural Science Foundation of Jiangsu Province [BK20200889]
- Chinese Postdoctoral Science Foundation [2019M661761]
- National Natural Science Foundation of China [21978120, 31670064]
- TaiShan Industrial Experts Program [tscy 20160101]
- scientific research project for college student in Jiangsu University [19A372]
Overexpressing xylose isomerase gene xylA or xylulokinase gene xks1 can significantly increase xylose utilization and enhance lipid content in the co-fermentation of glucose and xylose. The overexpression of xylA and/or xks1 is an effective strategy to improve the co-utilization of xylose and glucose, alleviate glucose repression, and produce lipids from lignocellulosic biomass in the oleaginous fungus M. circinelloides.
Most of the oleaginous microorganisms cannot assimilate xylose in the presence of glucose, which is the major bottleneck in the bioconversion of lignocellulose to biodiesel. Our present study revealed that overexpression of xylose isomerase (XI) gene xylA or xylulokinase (XK) gene xks1 increased the xylose consumption by 25 to 37% and enhanced the lipid content by 8 to 28% during co-fermentation of glucose and xylose. In xylA overexpressing strain Mc-XI, the activity of XI was 1.8-fold higher and the mRNA level of xylA at 24 h and 48 h was 11- and 13-fold higher than that of the control, respectively. In xks1 overexpressing strain Mc-XK, the mRNA level of xks1 was 4- to 11-fold of that of the control strain and the highest XK activity of 950 nmol min(-1) mg(-1) at 72 h which was 2-fold higher than that of the control. Additionally, expression of a translational fusion of xylA and xks1 further enhanced the xylose utilization rate by 45%. Our results indicated that overexpression of xylA and/or xks1 is a promising strategy to improve the xylose and glucose co-utilization, alleviate the glucose repression, and produce lipid from lignocellulosic biomass in the oleaginous fungus M. circinelloides.
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