Article
Multidisciplinary Sciences
Konstantinos Nakos, Md Noor A. Alam, Megan R. Radler, Ilona A. Kesisova, Changsong Yang, Joshua Okletey, Meagan R. Tomasso, Shae B. Padrick, Tatyana M. Svitkina, Elias T. Spiliotis
Summary: Cellular morphogenesis and processes such as cell division and migration require the coordination of the microtubule and actin cytoskeletons. This study shows that septins mediate microtubule-actin crosstalk by coupling actin polymerization to microtubule lattices.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Yolla German, Loan Vulliard, Anton Kamnev, Laurene Pfajfer, Jakob Huemer, Anna-Katharina Mautner, Aude Rubio, Artem Kalinichenko, Kaan Boztug, Audrey Ferrand, Joerg Menche, Loic Dupre
Summary: The immunological synapse decodes stimulatory signals into adapted lymphocyte responses, and high-content imaging is used to analyze T and NK cells. The study demonstrates how actin cytoskeleton remodeling shapes synapse architecture and affects lytic granule positioning. Analysis of immunodeficient individuals' CD8(+) T cells reveals the roles of ARPC1B and WASP in synapse assembly.
Article
Neurosciences
Samuel F. H. Barnett, Benjamin T. Goult
Summary: This study provides a new perspective on the understanding of biological digital information by analyzing the structure of MeshCODE in cells and proposing a possible gearbox mechanism for controlling synaptic transmission.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chanchanok Chaichim, Tamara Tomanic, Holly Stefen, Esmeralda Paric, Lucy Gamaroff, Alexandra K. Suchowerska, Peter W. Gunning, Yazi D. Ke, Thomas Fath, John Power
Summary: The study found that, despite being enriched in dendritic spines, overexpression of Tpm3.1 does not significantly alter the morphology or function of dendritic spines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biology
Rui Dong, Xuejun Li, Kwok-On Lai
Summary: PRMT8, among the nine mammalian protein arginine methyltransferases, stands out for its restricted expression in the nervous system and being the only membrane-bound PRMT. Studies have shown its involvement in various neuronal functions such as dendritic growth, synapse maturation, and synaptic plasticity. There is also emerging evidence suggesting a potential role of PRMT8 in neurological diseases.
Article
Biochemistry & Molecular Biology
Siwen Wu, Linxi Li, Xiaolong Wu, Chris K. C. Wong, Fei Sun, C. Yan Cheng
Summary: AKAP9 is a crucial regulatory protein in rat testes, supporting spermatogenesis by regulating MT and actin polymerization. Its function in Sertoli cells is essential for maintaining cytoskeleton homeostasis and adhesion between Sertoli cells and GC in the testis.
Review
Biology
John Gardiner
Summary: Life on Earth has evolved to adapt to a substantial gravitational field, so venturing into space requires a reevaluation of adaptability. Astronauts' physiological changes in space may be related to the cytoskeletal tensegrity architecture, affecting both physical and mental functions.
Review
Plant Sciences
Camila Goldy, Marie-Cecile Caillaud
Summary: Plants have developed cellular mechanisms to respond to signals by rearranging the cytoskeleton, regulated by phospholipids such as phosphatidic acid and phosphoinositides. This review focuses on the emerging role of phosphatidylinositol 4,5-bisphosphate in regulating the peripheral cytoskeleton during cellular processes.
CURRENT OPINION IN PLANT BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Tibor Szenasi, Gabor Turu, Laszlo Hunyady
Summary: beta-arrestins, initially known for their role in desensitizing rhodopsin and other G protein-coupled receptors, interact with the cytoskeletal system, including microtubules, actin filaments, and intermediate filaments. They regulate the activity of microtubules by recruiting signaling proteins and interacting with assembly proteins that control the actin cytoskeleton and intermediate filaments. Dysregulation of the cytoskeletal system is implicated in the development of neurodegenerative diseases, such as Alzheimer's and Parkinson's. Therefore, beta-arrestins are potential targets for future treatments of these diseases.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cell Biology
Pushpa Khanal, Pirta Hotulainen
Summary: Dendritic spines are small protrusions along neuronal dendrites where most excitatory synapses are located. The density of dendritic spines changes significantly during brain development, with alterations corresponding to aberrant brain function in various neurodevelopmental and neuropsychiatric disorders. BAR domain-containing proteins, MIM/Mtss1 and SrGAP3, are involved in spine initiation, but the potential roles of other BAR-domain proteins in this process require further exploration.
Article
Biology
Xudong Chen, Bowen Jia, Shihan Zhu, Mingjie Zhang
Summary: The volume and electric strength of an excitatory synapse are correlated with the area of its postsynaptic density (PSD). The molecular mechanism underlying the communication between the PSD assembly and spine actin cytoskeleton is poorly understood. This study discovers that PSD condensates can promote actin polymerization and bundling, and the Homer scaffold protein and a positively charged actin-binding surface of the Homer EVH1 domain are essential for this process. The communication between PSD and spine cytoskeleton may be modulated by targeting the phase separation of the PSD condensates.
Article
Cell Biology
Mira Kuzmic, Gerard Castro Linares, Jindriska Leischner Fialova, Francois Iv, Daniele Salaun, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Yuxiang Liu, Keisuke Asano, Magda Rodrigues, Daniel Isnardon, Taro Tachibana, Gijsje H. Koenderink, Ali Badache, Manos Mavrakis, Pascal Verdier-Pinard
Summary: This study identified the key molecular determinants and potential regulatory roles of septin-microtubule interaction, providing new insights into the mechanisms underlying septin-associated pathologies.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Beatrice Benoit, Christian Pous, Anita Baillet
Summary: The cytoskeleton is composed of different structures, including actin microfilaments, microtubules, intermediate filaments, and recently studied dynamic assemblies such as septins and the ESCRT complex. These filament-forming proteins control various cell functions through interactions with each other and with membranes. Recent research has focused on understanding how septins bind to membranes and influence their properties and functions, either directly or indirectly through other cytoskeleton elements.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
R. A. Degiosio, P. G. Needham, O. A. Andrews, H. Tristan, M. J. Grubisha, J. L. Brodsky, C. Camacho, R. A. Sweet
Summary: MAP2 phosphorylation plays a critical role in regulating cytoskeletal functions in neurons. Recent research has shown that phosphorylation of MAP2 in the proline-rich and C-terminal domains of the protein affects cellular functions including microtubule assembly, microtubule binding, actin binding, and actin polymerization.
Article
Cell Biology
Manos Mavrakis, M. Angeles Juanes
Summary: Cell movement is influenced by the turnover of focal adhesions, which traditionally was believed to be triggered by microtubules. However, recent research has discovered other molecular players that affect actin cytoskeleton dynamics, enabling directed cell migration.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Oncology
Johanna Schiewek, Udo Schumacher, Tobias Lange, Simon A. Joosse, Harriet Wikman, Klaus Pantel, Marina Mikhaylova, Matthias Kneussel, Stefan Linder, Barbara Schmalfeldt, Leticia Oliveira-Ferrer, Sabine Windhorst
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2018)
Article
Cell Biology
Andre R. A. Marques, Alessandro Di Spiezio, Niklas Thiessen, Lina Schmidt, Joachim Groetzinger, Renate Luellmann-Rauch, Markus Damme, Steffen E. Storck, Claus U. Pietrzik, Jens Fogh, Julia Baer, Marina Mikhaylova, Markus Glatzel, Mahmoud Bassal, Udo Bartsch, Paul Saftig
Article
Biochemistry & Molecular Biology
Bas van Bommel, Anja Konietzny, Oliver Kobler, Julia Baer, Marina Mikhaylova
Article
Cell Biology
Anja Konietzny, Judit Gonzalez-Gallego, Julia Baer, Alberto Perez-Alvarez, Alexander Drakew, Jeroen A. A. Demmers, Dick H. W. Dekkers, John A. Hammer, Michael Frotscher, Thomas G. Oertner, Wolfgang Wagner, Matthias Kneussel, Marina Mikhaylova
JOURNAL OF CELL SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Durga Praveen Meka, Robin Scharrenberg, Bing Zhao, Oliver Kobler, Theresa Koenig, Irina Schaefer, Birgit Schwanke, Sergei Klykov, Melanie Richter, Dennis Eggert, Sabine Windhorst, Carlos G. Dotti, Michael R. Kreutz, Marina Mikhaylova, Froylan Calderon de Anda
Article
Neurosciences
Chun Hu, Alexandros K. Kanellopoulos, Melanie Richter, Meike Petersen, Anja Konietzny, Federico M. Tenedini, Nina Hoyer, Lin Cheng, Carole L. C. Poon, Kieran F. Harvey, Sabine Windhorst, Jay Z. Parrish, Marina Mikhaylova, Claudia Bagni, Froylan Calderon de Anda, Peter Soba
JOURNAL OF NEUROSCIENCE
(2020)
Review
Cell Biology
Marina Mikhaylova, Jakob Rentsch, Helge Ewers
Article
Biology
Michael Bucher, Stephan Niebling, Yuhao Han, Dmitry Molodenskiy, Fatemeh Hassani Nia, Hans-Juergen Kreienkamp, Dmitri Svergun, Eunjoon Kim, Alla S. Kostyukova, Michael R. Kreutz, Marina Mikhaylova
Summary: Members of the SH3 and ankyrin repeat (SHANK) protein family are considered key scaffolds at glutamatergic synapses, with missense mutations in the canonical SHANK3 isoform potentially linked to ASD. This study demonstrates that ASD-associated point mutations in SHANK3 lead to changes in protein structure, resulting in altered synaptic targeting and protein turnover at synaptic sites in rat primary hippocampal neurons.
Article
Biochemistry & Molecular Biology
Anja Konietzny, Jasper Grendel, Alan Kadek, Michael Bucher, Yuhao Han, Nathalie Hertrich, Dick H. W. Dekkers, Jeroen A. A. Demmers, Kay Gruenewald, Charlotte Uetrecht, Marina Mikhaylova
Summary: Excitatory synapses of principal hippocampal neurons are often found on dendritic spines, with dynamic strengthening or weakening of individual inputs leading to structural and molecular diversity of spines. Caldendrin, a Ca2+ sensor, regulates the localization of ER tubules and formation of the spine apparatus in dendritic spines through a myosin V-dependent pathway, transforming myosin into a stationary F-actin tether.
Article
Cell Biology
Maximilian Borgmeyer, Cristina Coman, Canan Has, Hans-Frieder Schoett, Tingting Li, Philipp Westhoff, Yam F. H. Cheung, Nils Hoffmann, PingAn Yuanxiang, Thomas Behnisch, Guilherme M. Gomes, Mael Dumenieu, Michaela Schweizer, Michaela Chocholouskova, Michal Holcapek, Marina Mikhaylova, Michael R. Kreutz, Robert Ahrends
Summary: This study presents a quantitative lipid inventory of mouse and rat synaptic junctions, and develops a multiomics workflow to investigate the interplay of proteins and lipids in synaptic signal transduction. The findings reveal that environmental stimuli can regulate synaptic strength through altering protein expression in specific synapses. This compartment-specific multiomics workflow provides insights into complex lipid and protein networks involved in synaptic function and plasticity.
Review
Biochemistry & Molecular Biology
Julia Baer, Yannes Popp, Michael Bucher, Marina Mikhaylova
Summary: Microtubules possess changeable and tightly controlled properties, which are mediated by a variety of tubulin posttranslational modifications (PTMs) that either directly alter the MT lattice structurally or indirectly change MT interaction partners. Understanding the reversal and regulation of PTMs on MTs is crucial in deciphering their roles in cellular functions. Advances in microscopy techniques and live-sensors have greatly facilitated the study of PTM interactions and effects.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Anika Heinze, Cara Schuldt, Sharof Khudayberdiev, Bas van Bommel, Daniela Hacker, Toni G. Schulz, Ramona Stringhi, Elena Marcello, Marina Mikhaylova, Marco B. Rust
Summary: The study identified CAP1 as a novel regulator of the postsynaptic actin cytoskeleton that is essential for synaptic cofilin1 activity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Neurosciences
Anja Konietzny, Susanne Wegmann, Marina Mikhaylova
Summary: The heterogeneity of the endoplasmic reticulum (ER) allows it to function as a versatile platform for various homeostatic processes in neurons. This review focuses on the mechanisms that enable ER recruitment into dendritic spines, with a particular emphasis on transport and retention mechanisms. The role of calcium signaling, synaptopodin 'tagging', and the formation of the spine apparatus are discussed, along with the potential involvement of liquid-liquid phase separation.
TRENDS IN NEUROSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Balaganesh Kuruba, Nickolas Starks, Mary Rose Josten, Ori Naveh, Gary Wayman, Marina Mikhaylova, Alla S. Kostyukova
Summary: Dendritic spines, actin-rich protrusions that receive signals from axons at synapses, are affected by remodeling of cytoskeletal actin. Tropomodulin 2 (Tmod2), a brain-specific isoform, plays a role in regulating dendritic spine re-organization by binding to the pointed end of actin filaments through actin and tropomyosin binding sites. Overexpression of Tmod2 decreases spine number and increases spine length, and disrupting its binding abilities affects spine motility and stability.