Review
Biochemistry & Molecular Biology
Jara Tabitha Hees, Angelika Bettina Harbauer
Summary: Mitochondria play a critical role in neuronal function by providing ATP and buffering Ca2+. The regulation of mitochondrial biogenesis at the transcriptional and translational level is necessary for neurons to adapt to local differences in mitochondrial demand. Defects in the localization of mRNAs encoding mitochondrial proteins and contact site formation between mitochondria and endolysosomes can lead to mitochondrial dysfunction in various neurological disorders.
Article
Biochemistry & Molecular Biology
Madison E. Rivera, Roger A. Vaughan
Summary: The study found that excess lipid and excess insulin can lead to reduced mitochondrial metabolism in myotubes, with an increase in glycolytic metabolism. These stressors independently have negative effects on insulin signaling, mitochondrial function, and cell metabolism, while the combined treatment has a more significant impact on metabolic regulators.
Article
Immunology
Abeer Elkhoely
Summary: The present study investigated the impact of liraglutide on gentamicin-induced kidney injury in rats. The results showed that liraglutide alleviated renal injury markers, oxidative stress, inflammation, and apoptosis. This study also revealed the involvement of mitochondrial biogenesis and its upstream regulators in the nephroprotective effects of liraglutide.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ying Jiang, Da Li, Zhiqiang Du, Jie Li, Rongrong Lu, Qin Zhou, Qi Wang, Haohao Zhu
Summary: The study investigated the protective effects of Perampanel on mitochondrial biogenesis and bioenergetics in human neuronal cells. Results showed that Perampanel stimulated mitochondrial biogenesis and improved mitochondrial bioenergetics.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yi Li, Yu-Fan Feng, Xiao-Tian Liu, Yu-Chen Li, Hui-Min Zhu, Meng-Ru Sun, Ping Li, Baolin Liu, Hua Yang
Summary: Songorine protects cardiac contractive function against endotoxin insult by activating Nrf2, suppressing mitochondrial reactive oxygen species production, and promoting mitochondrial biogenesis, ultimately enhancing cardioprotection.
Article
Medicine, Research & Experimental
Yang Cao, Lingyi Tang, Kang Du, Kitt Paraiso, Qiushi Sun, Zhengxia Liu, Xiaolong Ye, Yuan Fang, Fang Yuan, Hank Chen, Yumay Chen, Xiaorong Wang, Clinton Yu, Ira L. Blitz, Ping H. Wang, Lan Huang, Haibo Cheng, Xiang Lu, Ken W. Y. Cho, Marcus Seldin, Zhuyuan Fang, Qin Yang
Summary: LSD1 regulates mitochondrial function and hepatokine production by modulating histone methylation and NAD+ synthesis. Deletion of LSD1 in mice reduces mitochondrial function in the liver but protects against diet-induced hepatic steatosis and glucose intolerance, partially due to the induction of the hepatokine FGF21.
Article
Cell Biology
Peng Ye, Wei Li, Xin Huang, Sheng Zhao, Wu Chen, Yuqi Xia, Weimin Yu, Ting Rao, Jinzhuo Ning, Xiangjun Zhou, Yuan Ruan, Fan Cheng
Summary: This study aimed to investigate the role and mechanism of BMAL1 in renal ischemia-reperfusion injury (IRI). The results showed that BMAL1 mediates mitochondrial homeostasis in renal IRI through the SIRT1/PGC-1 alpha axis, providing a new direction for targeted therapy for renal IRI.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Food Science & Technology
Ziyuan Wang, Qing Li, Yiming Hao, Zongwei Wang, Haihong Yang, Jie Liu, Jing Wang
Summary: AR-C17 significantly improved high-fat diet-induced skeletal muscle dysfunction in mice by enhancing mitochondrial biogenesis, without affecting myofiber type conversion or myogenesis.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Daniel Da Eira, Shailee Jani, Mateja Stefanovic, Rolando B. Ceddia
Summary: The purpose of this study was to determine the effects of the ketogenic diet on weight reduction and fat burning by examining the energy-dissipating pathways in brown adipose tissue (BAT) and white adipose tissue (WAT). The results showed that the ketogenic diet preserved insulin sensitivity and lipolytic capacity in WAT, and increased energy-dissipating pathways in BAT, but did not prevent an increase in adiposity.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Qing Zheng, Hanghang Liu, Hao Zhang, Yaobao Han, Jiaxin Yuan, Tingting Wang, Yifan Gao, Zhen Li
Summary: This study reports the use of Cu2-xSe-based nanoparticles functionalized with curcumin and wrapped with DSPE-PEG(2000)-TPP-modified macrophage membrane (CSCCT NPs) to promote mitochondrial biogenesis and ameliorate mitochondrial dysfunction for the treatment of Parkinson's disease.
Article
Cell Biology
Ruzhou Zhao, Yixin Xu, Xiaobo Wang, Xiang Zhou, Yanqi Liu, Shuai Jiang, Lin Zhang, Zhibin Yu
Summary: In extremely hypoxic environments, WA can protect cells by affecting the mitophagy and mitochondrial biogenesis pathways to reduce myocardial damage, maintain cardiac function, and improve survival. Enhanced mitophagy eliminates damaged mitochondria and prevents apoptosis induction, while enhanced mitochondrial biogenesis can supplement functional mitochondria and maintain mitochondrial respiration to ensure mitochondrial ATP production under acute extreme hypoxia.
Article
Biochemistry & Molecular Biology
Mariantonietta Capristo, Valentina Del Dotto, Concetta Valentina Tropeano, Claudio Fiorini, Leonardo Caporali, Chiara La Morgia, Maria Lucia Valentino, Monica Montopoli, Valerio Carelli, Alessandra Maresca
Summary: The study suggests that rapamycin-based therapy may be a promising treatment option for MERRF. The first therapeutic strategy was ineffective in high-mutation load cell lines, while the second strategy showed significant improvement in fibroblasts with intermediate-mutation load, rescuing the bioenergetics defect completely.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mattia Quattrocelli, Michelle Wintzinger, Karen Miz, Daniel C. Levine, Clara Bien Peek, Joseph Bass, Elizabeth M. McNally
Summary: This study found that the timing of glucocorticoid intake can affect their effects on muscle bioenergetics. In mice, prednisone dosing during the light phase promoted NAD(+) levels and mitochondrial function, while dosing during the dark phase did not have the same effect. These results suggest that the circadian clock and muscle PGC1 alpha activity are important factors in glucocorticoid chronopharmacology for muscle bioenergetics.
Article
Biochemistry & Molecular Biology
Boyu Yang, Qiongli Yu, Bo Chang, Qi Guo, Sitong Xu, Xuejie Yi, Shicheng Cao
Summary: The study demonstrates the potential of AMPK/PGC-1 alpha pathway in mediating the secretion and/or production of MOTS-c in skeletal muscle, suggesting the roles of exercise intervention and recombinant MOTS-c in treating obesity and diabetes mellitus.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Plant Sciences
Chia-Yuan Lin, Yan-Ning Huang, Ru-Huei Fu, Yu-Hsin Liao, Tzu-Yu Kuo, Chia-Wen Tsai
Summary: The neuroprotective mechanism of carnosic acid (CA) from rosemary may involve the regulation of PARIS and PGC-1 alpha expression, enhancing mitochondrial biogenesis to prevent the pathological progression of Parkinson's disease.
Article
Cell Biology
Meghan G. Patton, Trevor L. Gillum, Mandy C. Szymanski, Lacey M. Gould, Claire J. Lauterbach, Roger A. Vaughan, Matthew R. Kuennen
CELL STRESS & CHAPERONES
(2018)
Review
Food Science & Technology
Nicholas P. Gannon, Jamie K. Schnuck, Roger A. Vaughan
MOLECULAR NUTRITION & FOOD RESEARCH
(2018)
Article
Nutrition & Dietetics
Emily S. Lyon, Madison E. Rivera, Michele A. Johnson, Kyle L. Sunderland, Roger A. Vaughan
NUTRITION RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Madison E. Rivera, Emily S. Lyon, Roger A. Vaughan
JOURNAL OF CELLULAR BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Chad M. Paton, Yura Son, Roger A. Vaughan, Jamie A. Cooper
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Endocrinology & Metabolism
Madison E. Rivera, Caroline N. Rivera, Roger A. Vaughan
Summary: The study aimed to examine the effect of a BCAA mixture on muscle insulin signaling in vitro, showing that BCAA treatment could reduce basal insulin signaling in healthy cells and insulin-stimulated insulin signaling in insulin resistant cells. However, further investigation is needed to determine the extent of these observations in vivo models.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Madison E. Rivera, Roger A. Vaughan
Summary: The study found that excess lipid and excess insulin can lead to reduced mitochondrial metabolism in myotubes, with an increase in glycolytic metabolism. These stressors independently have negative effects on insulin signaling, mitochondrial function, and cell metabolism, while the combined treatment has a more significant impact on metabolic regulators.
Article
Biochemistry & Molecular Biology
Martina J. Krone, Caroline N. Rivera, Madison E. Rivera, Rachel M. Watne, Sarah E. Lemonds, Andrew J. Wommack, Roger A. Vaughan
Summary: This study found that supraphysiological concentrations of glutamine have no significant effect on myotube metabolism and insulin signaling, while physiological levels of glutamine can improve metabolism.
Article
Biochemistry & Molecular Biology
Jason S. Hinkle, Caroline N. Rivera, Roger A. Vaughan
Summary: Type 2 diabetes is characterized by reduced insulin sensitivity, elevated blood metabolites, and reduced mitochondrial metabolism. This study investigated the effects of the AMPK activator AICAR on the metabolism and expression of BCAA catabolic enzymes in cultured myotubes. The results demonstrated that AICAR treatment increased mitochondrial content, AMPK activation, and the overall BCAA catabolic capacity in skeletal muscle cells. These findings suggest that AMPK activation plays a role in regulating BCAA metabolism and may have implications for the study of type 2 diabetes.
Article
Cell Biology
Caroline N. Rivera, Madison M. Kamer, Madison E. Rivera, Rachel M. Watne, Trent C. Macgowan, Andrew J. Wommack, Roger A. Vaughan
Summary: Insulin resistance, but not BCAA treatment, promotes extracellular BCAA accumulation independent of changes in LAT1 content, suggesting insulin resistance as a causal agent of extracellular BCAA accumulation.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Review
Food Science & Technology
Jason S. Hinkle, Caroline N. Rivera, Roger A. Vaughan
Summary: BCAA are essential in the diet and have effects on cell energetics, especially in mitochondrial biogenesis. However, the physiological relevance of these findings in humans is still unclear. Further well-controlled diet studies are needed to evaluate the association between BCAA consumption and increased mitochondrial biogenesis and improved metabolic outcomes.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Caroline N. N. Rivera, Rachel M. M. Watne, Zoe A. A. Brown, Samantha A. A. Mitchell, Andrew J. J. Wommack, Roger A. A. Vaughan
Summary: Individuals with insulin resistance often have elevated circulating branched-chain amino acids (BCAA) due to reduced BCAA breakdown capacity. This study examined the impact of AMPK activation on LAT1 expression, the primary BCAA transporter, in a skeletal muscle cell model. The findings suggest that AMPK activation can reduce LAT1 expression and BCAA uptake, particularly in a glucose-dependent manner.
Article
Endocrinology & Metabolism
Madison E. Rivera, Caroline N. Rivera, Roger A. Vaughan
Summary: The study indicates that supraphysiologically high levels of BCAA may negatively impact mitochondrial metabolism, and concurrent insulin resistance may diminish peak mitochondrial capacity and impede molecular adaptations supporting a transition to a glycolytic preference.
Article
Biochemistry & Molecular Biology
Michele A. Johnson, Nicholas P. Gannon, Jamie K. Schnuck, Emily S. Lyon, Kyle L. Sunderland, Roger A. Vaughan
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.