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Biochemistry & Molecular Biology
Zhihao Wang, Zhicheng Pan, Samir Adhikari, Bryan T. Harada, Lei Shen, Wei Yuan, Tharindumala Abeywardana, Qais Al-Hadid, Jeremy M. Stark, Chuan He, Lan Lin, Yanzhong Yang
Summary: The research reveals that arginine methylation of METTL14 is a novel pathway controlling m(6)A deposition in mammalian cells, with deficiency in this methylation leading to significantly reduced global m(6)A levels in mESCs. These findings highlight the importance of arginine methylation in RNA metabolism and its impact on various cellular processes, including stem cell maintenance and DNA repair.
Article
Cell Biology
Alicia Sampieri, Alexander Asanov, Kevin Manuel Mendez-Acevedo, Luis Vaca
Summary: This study reveals that SIDT2 forms a complex with ApoA1 and enhances its secretion. The second CRAC-2 motif in SIDT2 is crucial for the formation of this complex.
Review
Biochemistry & Molecular Biology
Jennifer J. Lee, Vaibhav Jain, Ravi K. Amaravadi
Summary: Despite advances in targeted therapy and immunotherapy, treating RAS mutant cancers remains challenging. These cancers have been found to activate adaptive resistance mechanisms like autophagy during MAPK inhibition. Recent focus has been on developing MAPK pathway and autophagy inhibitors for potential synthetic lethal approaches in cancer therapy, with ongoing clinical trials to evaluate their efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yingying Song, Jing Gu, Jingya You, Yiyang Tao, Yao Zhang, Lizhuo Wang, Jialin Gao
Summary: Sidt2 is a lysosomal membrane protein that plays a crucial role in maintaining the normal morphology and stability of lysosomes, as well as regulating glucose and lipid metabolism and nucleotide transport.
Article
Biochemistry & Molecular Biology
Rachel Curnock, Katy Yalci, Johan Palmfeldt, Marja Jaattela, Bin Liu, Bernadette Carroll
Summary: The accumulation of senescent cells is a driver of aging, and one characteristic of senescent cells is an increase in lysosomal content. However, little is known about the causes and consequences of lysosomal biogenesis in senescence.
Article
Biochemistry & Molecular Biology
Lizhuo Wang, Cui Yu, Wenjun Pei, Mengya Geng, Yao Zhang, Zihui Li, Feiteng Liang, Fengbiao Tan, Hui Du, Jialin Gao
Summary: The study revealed that deletion of the Sidt2 gene disrupted the quality control of mitochondria, inhibited the clearance of mitochondria, resulting in the accumulation of damaged mitochondria, ultimately leading to the abnormal structure and function of skeletal muscle.
Article
Food Science & Technology
Mengting Shang, Shuyan Niu, Xiaoru Chang, Jiangyan Li, Wenli Zhang, Menghao Guo, Tianshu Wu, Ting Zhang, Meng Tang, Yuying Xue
Summary: This study evaluated the effects of silver nanoparticles (AgNPs) on different stages of autophagy and found that they induced neuroinflammation and blocked autophagic flux. AgNPs hindered autophagic flux by inhibiting the fusion between autophagosomes and lysosomes, aggravating the neurotoxicity caused by AgNPs.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shunichi Hatamiya, Masatsugu Miyara, Yaichiro Kotake
Summary: Tributyltin (TBT), an environmental pollutant, has been found to be toxic to mammals. This study investigated the mechanism by which TBT inhibits autophagic degradation in SH-SY5Y cells. The results showed that TBT reduced the acidity of lysosomes without affecting lysosomal membrane integrity, leading to the impairment of autophagy. This finding suggests that autophagy impairment may be involved in TBT-induced neuronal death and T-cell-dependent thymus atrophy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Merve Kacal, Boxi Zhang, Yuqing Hao, Erik Norberg, Helin Vakifahmetoglu-Norberg
Summary: This study utilized multiplex quantitative mass spectrometry and bioinformatics analysis to investigate the proteome of isolated lysosomes under non-macroautophagy-activated conditions, shedding light on the functional dependency of LAMP2A and the influence of KFERQ-like motifs in proteins on lysosomal targeting.
Article
Multidisciplinary Sciences
Xavier Leray, Rossella Conti, Yan Li, Cecile Debacker, Florence Castelli, Francois Fenaille, Anselm A. Zdebik, Michael Pusch, Bruno Gasnier
Summary: This study quantitatively investigated the transport activity of PQLC2 and revealed that it operates in a uniporter mode, uncoupled from the steep lysosomal proton gradient, enabling bidirectional cationic amino acid transport. It was also discovered that the specific presence of arginine in the discharge compartment impaired PQLC2 transport activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Immunology
Chieko Matsui, Putu Yuliandari, Lin Deng, Takayuki Abe, Ikuo Shoji
Summary: Lysosomes degrade proteins through autophagy, with CMA being a selective degradation pathway using the KFERQ motif. Hepatitis C virus NS5A interacts with HSC70 and HNF-1α to promote CMA-induced lysosomal degradation, contributing to HCV pathogenesis. Further study of CMA-targeting proteins interacting with HCV NS5A may provide insights into HCV-induced pathogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Menglan He, Alvin C. Y. Kuk, Mei Ding, Cheen Fei Chin, Dwight L. A. Galam, Jie Min Nah, Bryan C. Tan, Hui Li Yeo, Geok Lin Chua, Peter I. Benke, Markus R. Wenk, Lena Ho, Federico Torta, David L. Silver
Summary: This study identifies a new lysosomal transporter called Spns1, which plays a crucial role in the transport of lysophosphatidylcholine and lysophosphatidylethanolamine, maintaining normal lysosomal function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Jing Crystal Zhao, Aurian Saleh, Stanley T. Crooke
Summary: The lysosome protein SIDT2 regulates the activity of PS-modified antisense oligonucleotides (PS-ASOs) by interacting with microtubule-related proteins to place lysosomes in perinuclear regions, facilitating PS-ASOs' localization and degradation.
NUCLEIC ACID THERAPEUTICS
(2023)
Article
Multidisciplinary Sciences
Yuchen Feng, Aileen R. Ariosa, Ying Yang, Zehan Hu, Joern Dengjel, Daniel J. Klionsky
Summary: Macroautophagy/autophagy is a crucial molecular process for maintaining cellular homeostasis, recycling cytoplasmic materials, and responding to stress conditions. The posttranslational modification of Atg9, including ubiquitination and proteasome-dependent degradation, regulates the level of autophagy in cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Celia A. McKee, Alexander J. Polino, Melvin W. King, Erik S. Musiek
Summary: The circadian clock is found to play a new role in autophagy and lysosome function, suggesting new directions for studying neurodegeneration. Circadian clock proteins coordinate gene expression programs involved in daily rhythms and cellular processes. The depletion of the core clock protein BMAL1 in astrocytes disrupts circadian function and leads to a unique cell-autonomous activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
So Masaki, Shun Ikeda, Asuka Hata, Yusuke Shiozawa, Ayana Kona, Seishi Ogawa, Kenji Suzuki, Fumihiko Hakuno, Shin-Ichiro Takahashi, Naoyuki Kataoka
FRONTIERS IN GENETICS
(2019)
Article
Cell Biology
So Masaki, Takafumi Kabuto, Kenji Suzuki, Naoyuki Kataoka
Article
Oncology
Ru Chen, Aiko Sugiyama, Naoyuki Kataoka, Masahiro Sugimoto, Shoko Yokoyama, Akihisa Fukuda, Shigeo Takaishi, Hiroshi Seno
FRONTIERS IN ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
So Masaki, Kozue Hashimoto, Daiki Kihara, Chizuru Tsuzuki, Naoyuki Kataoka, Kenji Suzuki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Shintaro Muraoka, Kazuhiro Fukumura, Megumi Hayashi, Naoyuki Kataoka, Akila Mayeda, Daisuke Kaida
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Takahito Nakura, Atsufumi Ozoe, Yuka Narita, Masafumi Matsuo, Fumihiko Hakuno, Naoyuki Kataoka, Shin-Ichiro Takahashi
Summary: This study reveals that RbFox2 and SRSF3 proteins mediate exon 11 inclusion in rat hepatoma cells, with the UGCAUG sequence serving as a key regulator for exon 11 inclusion.
Article
Cell Biology
Brian H. Kim, Maciej Jeziorek, Hur Dolunay Kanal, Viorica Raluca Contu, Radek Dobrowolski, Steven W. Levison
Summary: Inhibiting ALK5 can reduce cell death and improve neurological function, potentially through increasing levels of autophagy markers.
Review
Biochemistry & Molecular Biology
Naoyuki Kataoka, Eri Matsumoto, So Masaki
Summary: Pre-mRNA splicing is a crucial process for gene expression in higher eukaryotes, and mutations in splicing factors can lead to various human diseases, including MDS. Recent studies have shown that mutations in splicing factors play a driver role in human cancers, affecting about 50% of MDS patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sara Fukushima, Hiroki Nishi, Mikako Kumano, Daisuke Yamanaka, Naoyuki Kataoka, Fumihiko Hakuno, Shin-Ichiro Takahashi
Summary: Amino acids act as metabolic regulatory signals, directly controlling G6Pase mRNA levels through transcriptional regulation independent of hormone signaling pathways. Amino acid starvation affects the urea cycle, while supplementation of ornithine can fully rescue G6Pase mRNA transcription.
Article
Biochemistry & Molecular Biology
Shogo Taniguchi, Yukiko Ito, Hibiki Kiritani, Asuka Maruo, Ryohei Sakai, Yuji Ono, Ryosuke Fukuda, Tsukasa Okiyoneda
Summary: The peripheral protein quality control (periQC) system eliminates conformationally defective cystic fibrosis transmembrane conductance regulator (CFTR) and limits the efficacy of pharmacological therapy for cystic fibrosis (CF). In this study, it was found that the ubiquitin ligase RNF34 participates in CFTR periQC along with RFFL. RNF34 directly recognizes CFTR NBD1 and promotes the ubiquitination of unfolded proteins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Viorica Raluca Contu, Ryohei Sakai, Yuuki Fujiwara, Chihana Kabuta, Keiji Wada, Tomohiro Kabuta
Summary: RNautophagy/DNautophagy (RDA) is an autophagic process in which nucleic acids are taken up by lysosomes for degradation. This study found that the acidic pH of lysosomes does not interfere with the uptake of nucleic acids during RDA. Inhibition of V-ATPase or knockdown of Atp6v1a did not affect the ability of lysosomes to take up RNA, indicating that V-ATPase is not required for the uptake process. It was also demonstrated that ATP consumption is necessary for nucleic acid uptake by lysosomes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)