Review
Chemistry, Multidisciplinary
Benjamin Emenike, Ogonna Nwajiobi, Monika Raj
Summary: Nature increases the functional diversity of the proteome through posttranslational modifications (PTMs), which can be correlated to diseases. Therefore, it is important to qualitatively and quantitatively detect these transformations. One effective method is to use chemical strategies to label different PTMs, facilitating the study of their roles.
FRONTIERS IN CHEMISTRY
(2022)
Review
Biochemical Research Methods
Yanling Zhang, Wei Qin, Chu Wang
Summary: Metabolic reprogramming of macrophages during immune activation leads to the production of diverse small molecule metabolites that induce post-translational modifications on proteins. Recent advances in chemical proteomics have enabled precise identification of these modifications, facilitating the understanding of their functional implications.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Elise Loppinet, Harrison A. Besser, Agnele Sylvia Sewa, Fu-Chen Yang, Bana Jabri, Chaitan Khosla
Summary: Celiac disease is caused by gluten-derived antigens triggering inflammation through presenting to CD4+ T cells. In this study, we propose a mechanism for the deamidation and concentration of gluten peptides in the lysosomes of antigen-presenting cells, which explains the high concentration of gluten peptides required for an inflammatory response in CeD patients. We found that a ternary complex formed between a gluten peptide, transglutaminase-2 (TG2), and a2-macroglobulin, which was endocytosed by LRP-1. The covalent TG2-peptide adduct underwent endolysosomal decoupling, resulting in the expected deamidated epitope. Our findings suggest a pathogenic role for dendritic cells and/or macrophages in CeD and implicate TG2 in the lysosomal clearance of unwanted proteins.
CELL CHEMICAL BIOLOGY
(2023)
Review
Physiology
Kelsey S. Kalous, Sarah L. Wynia-Smith, Brian C. Smith
Summary: Increased sirtuin deacylase activity is associated with longer lifespan and lower susceptibility to aging-related diseases in eukaryotes, while decreased activity is linked to higher disease risk. Oxidative post-translational modifications such as cysteine nitrosation and glutathionylation can inhibit sirtuin deacylase activity, potentially providing a mechanistic link between cellular oxidants and age-related disease development.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Eugene Varfolomeev, Domagoj Vucic
Summary: Receptor interacting protein 1 (RIP1) kinase is a critical regulator of inflammation and cell death signaling, and its post-translational modifications, such as ubiquitination, phosphorylation, and cleavage, greatly impact its function in signaling pathways.
BIOCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Julia Kraxner, Charlotta Lorenz, Julia Menzel, Iwan Parfentev, Ivan Silbern, Manuela Denz, Henning Urlaub, Blanche Schwappach, Sarah Koester
Summary: The mechanical properties of biological cells are influenced by the cytoskeleton, with phosphorylation serving as a fast mechanism for mechanical modulation. Phosphorylation can soften filaments, and the binding of 14-3-3 protein to phosphorylated filaments further enhances this effect, potentially preserving the softening and altering cell mechanics in the cell.
Article
Cell Biology
Mohd Shariq, Neha Quadir, Javaid Ahmad Sheikh, Alok Kumar Singh, William R. Bishai, Nasreen Z. Ehtesham, Seyed E. Hasnain
Summary: The host utilizes ubiquitin pathway to combat intracellular pathogens, while pathogens like Mycobacterium tuberculosis exploit this pathway to dampen host innate immune response.
Review
Cell Biology
Li Chen, Anna Kashina
Summary: Post-translational modifications (PTM) involve enzyme-mediated covalent addition of functional groups to proteins during or after synthesis, greatly increasing biological complexity and playing a crucial role in biological regulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Multidisciplinary Sciences
Ji Min Lee, Henrik M. Hammaren, Mikhail M. Savitski, Sung Hee Baek
Summary: Post-translational modifications (PTMs) on specific amino acids control the stability of target proteins. These PTM-regulated degrons act as signals for protein degradation or stabilization. This review summarizes the current knowledge of PTM-mediated protein stability regulation to enhance the identification of novel drug targets.
NATURE COMMUNICATIONS
(2023)
Review
Cell Biology
Rohan Gupta, Mehar Sahu, Devesh Srivastava, Swati Tiwari, Rashmi K. Ambasta, Pravir Kumar
Summary: Post-translational modifications play a crucial role in the pathogenesis of neurodegenerative diseases by regulating protein homeostasis and signaling cascades, leading to enhanced neuroprotection and potentially reversing misfolded protein accumulation.
AGEING RESEARCH REVIEWS
(2021)
Review
Cell Biology
Linbin Zhou, Danny Siu-Chun Ng, Jason C. Yam, Li Jia Chen, Clement C. Tham, Chi Pui Pang, Wai Kit Chu
Summary: This review summarizes recent findings on the impact of post-translational modifications (PTMs) on the functions of retinoblastoma protein (pRb). PTMs on pRb play crucial roles in cell cycle regulation, cell survival, and differentiation, and altered PTMs can lead to anomalies in cell proliferation and tumorigenesis. Understanding the post-translational modulation of pRb could facilitate the development of novel therapeutic strategies for various human diseases.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Review
Cell Biology
Takashi Akera
Summary: Haploid gametes are generated from diploid parents via meiosis, a process essential for sexually reproducing eukaryotes. While the precise mechanism of faithful chromosome segregation in meiosis is not fully understood, it is increasingly evident that tubulin post-translational modifications (PTMs) play crucial roles in regulating microtubule functions during meiosis. This review highlights recent advances in understanding the roles of tubulin PTMs in meiotic spindles, particularly their involvement in spindle integrity, oocyte aging, and non-Mendelian inheritance.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Faith M. Joseph, Nicolas L. Young
Summary: Post-translational modifications (PTMs) of histones play a key role in DNA-based processes and contribute to cell differentiation and gene function by adding an extra layer of regulation. Variations in histone sequences within each family of histones expand the chromatin repertoire and provide further mechanisms for regulation and signaling. However, much remains unknown about variant-specific PTMs and their role in regulating chromatin due to limited technologies and appropriate reagents.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yanxiang Meng, Jarrod J. Sandow, Peter E. Czabotar, James M. Murphy
Summary: This article discusses the role of phosphorylation, ubiquitylation, GlcNAcylation, proteolytic cleavage, and disulfide bonding in regulating necroptotic signaling, with a focus on RIPK1, RIPK3, and MLKL. Understanding of these post-translational modifications can be valuable in efforts to therapeutically modulate necroptosis.
CELL DEATH AND DIFFERENTIATION
(2021)
Review
Biochemistry & Molecular Biology
Ivana Samarzija
Summary: The specific functional form of a protein is mostly achieved through dynamic interactions of many enzymes in post-translational modifications, which cells widely use to respond to stimuli, regulate transcription, and maintain proteostasis. Post-translational modifications play important roles in driving prostate cancer, particularly in regulating protein activity at the level of post-translational modifications.
Article
Cell Biology
Saborni Chakraborty, Joseph C. Gonzalez, Benjamin L. Sievers, Vamsee Mallajosyula, Srijoni Chakraborty, Megha Dubey, Usama Ashraf, Bowie Yik-Ling Cheng, Nimish Kathale, Kim Quyen Thi Tran, Courtney Scallan, Aanika Sinnott, Arianna Cassidy, Steven T. Chen, Terri Gelbart, Fei Gao, Yarden Golan, Xuhuai Ji, Seunghee Kim-Schulze, Mary Prahl, Stephanie L. Gaw, Sacha Gnjatic, Thomas U. Marron, Miriam Merad, Prabhu S. Arunachalam, Scott D. Boyd, Mark M. Davis, Marisa Holubar, Chaitan Khosla, Holden T. Maecker, Yvonne Maldonado, Elizabeth D. Mellins, Kari C. Nadeau, Bali Pulendran, Upinder Singh, Aruna Subramanian, Paul J. Utz, Robert Sherwood, Sheng Zhang, Prasanna Jagannathan, Gene S. Tan, Taia T. Wang
Summary: A damaging inflammatory response in severe COVID-19 is associated with early non-neutralizing, afucosylated IgG antibodies specific to SARS-CoV-2. Human IgG-Fc-gamma receptor interactions regulate inflammation in the lung, and antibodies elicited by mRNA SARS-CoV-2 vaccines have reduced inflammatory potential.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Brad A. Palanski, Nielson Weng, Lichao Zhang, Andrew J. Hilmer, Lalla A. Fall, Kavya Swaminathan, Bana Jabri, Carolina Sousa, Nielsen Q. Fernandez-Becker, Chaitan Khosla, Joshua E. Elias
Summary: In this study, a new technique was used to discover various dietary peptides in urine. These peptides play important roles in celiac disease-related T cell and innate immune responses, and have qualitative and quantitative differences in patients with celiac disease compared to healthy individuals. These findings are significant for understanding gluten immunogenicity, improving celiac disease management, and characterizing the dietary and urinary peptidomes.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Marisa Holubar, Aruna Subramanian, Natasha Purington, Haley Hedlin, Bryan Bunning, Katharine S. Walter, Hector Bonilla, Athanasia Boumis, Michael Chen, Kimberly Clinton, Liisa Dewhurst, Carol Epstein, Prasanna Jagannathan, Richard H. Kaszynski, Lori Panu, Julie Parsonnet, Elizabeth L. Ponder, Orlando Quintero, Elizabeth Sefton, Upinder Singh, Luke Soberanis, Henry Truong, Jason R. Andrews, Manisha Desai, Chaitan Khosla, Yvonne Maldonado
Summary: This study found that commonly used doses of favipiravir did not show significant effectiveness in treating uncomplicated COVID-19 in outpatient settings. Further research is needed to determine if higher doses of favipiravir are effective and safe for patients with COVID-19.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Jing Li, Maxim Zaslavsky, Yapeng Su, Jing Guo, Michael J. Sikora, Vincent van Unen, Asbjorn Christophersen, Shin-Heng Chiou, Liang Chen, Jiefu Li, Xuhuai Ji, Julie Wilhelmy, Alana M. McSween, Brad A. Palanski, Venkata Vamsee Aditya Mallajosyula, Nathan A. Bracey, Gopal Krishna R. Dhondalay, Kartik Bhamidipati, Joy Pai, Lucas B. Kipp, Jeffrey E. Dunn, Stephen L. Hauser, Jorge R. Oksenberg, Ansuman T. Satpathy, William H. Robinson, Cornelia L. Dekker, Lars M. Steinmetz, Chaitan Khosla, Paul J. Utz, Ludvig M. Sollid, Yueh-Hsiu Chien, James R. Heath, Nielsen Q. Fernandez-Becker, Kari C. Nadeau, Naresha Saligrama, Mark M. Davis
Summary: The study reveals that CD8(+) T cells expressing inhibitory receptors play a regulatory role in immune responses in both humans and mice, and their increased presence is observed in various autoimmune diseases and COVID-19 patients. The findings suggest that these regulatory CD8(+) T cells are involved in suppressing pathogenic T cells in autoimmune and infectious diseases.
Review
Biotechnology & Applied Microbiology
Katarina M. Guzman, Chaitan Khosla
Summary: This review discusses the versatility of fragment-antigen binding domains (F(ab)s) as protein crystallization chaperones and describes a high-throughput workflow for identifying F(ab)s to aid the crystallization of proteins of interest. The use of F(ab)s has facilitated the crystallization of challenging proteins and improved particle quality for cryo-electron microscopy.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Biology
Zicheng Hu, Kattria van der Ploeg, Saborni Chakraborty, Prabhu S. Arunachalam, Diego A. M. Mori, Karen B. Jacobson, Hector Bonilla, Julie Parsonnet, Jason R. Andrews, Marisa Holubar, Aruna Subramanian, Chaitan Khosla, Yvonne Maldonado, Haley Hedlin, Lauren de la Parte, Kathleen Press, Maureen Ty, Gene S. Tan, Catherine Blish, Saki Takahashi, Isabel Rodriguez-Barraquer, Bryan Greenhouse, Atul J. Butte, Upinder Singh, Bali Pulendran, Taia T. Wang, Prasanna Jagannathan, Miles P. Davenport
Summary: Using host proteomics and transcriptomics, the trajectory of immune response in COVID-19 patients was characterized. Early immune markers were found to be associated with disease progression, control of viral shedding, and SARS-CoV-2-specific T cell and antibody responses. Machine-learning models accurately predicted patient outcomes.
Article
Biochemistry & Molecular Biology
Jake Hsu, Nina Fatuzzo, Nielson Weng, Wojciech Michno, Wentao Dong, Maryline Kienle, Yuqin Dai, Anca Pasca, Monther Abu-Remaileh, Natalie Rasgon, Benedetta Bigio, Carla Nasca, Chaitan Khosla
Summary: In eukaryotes, carnitine plays a crucial role in shuttling fatty acids and acetyl groups across mitochondrial membranes for energy production and cellular processes. However, the mechanism by which exogenous LAC affects cellular metabolism is not well understood.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Immunology
Katharine S. Walter, Eugene Kim, Renu Verma, Jonathan Altamirano, Sean Leary, Yuan J. Carrington, Prasanna Jagannathan, Upinder Singh, Marisa Holubar, Aruna Subramanian, Chaitan Khosla, Yvonne Maldonado, Jason R. Andrews
Summary: This study found that there is limited genetic diversity among SARS-CoV-2 samples from the same household, while individuals infected with the same viral strain from different households have lower genetic similarity. Shared within-host variations in a household are significantly associated with household membership. However, current sequencing and bioinformatic workflows may not consistently detect low-frequency within-host variants.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Katarina M. Guzman, Dillon P. Cogan, Krystal L. Brodsky, Alexander M. Soohoo, Xiuyuan Li, Natalia Sevillano, Irimpan I. Mathews, Khanh P. Nguyen, Charles S. Craik, Chaitan Khosla
Summary: This study discovered and characterized two F(ab) antibodies (AC2 and BB1) that potently inhibit the catalytic activity of the ketosynthase-acyltransferase (KS-AT) core of Module 2 in the synthesis of 6-deoxyerythronolide B synthase (DEBS). The antibodies were found to modulate ACP-mediated reactions catalyzed by Module 2 through different mechanisms. Another F(ab) antibody, AA5, was identified to bind to the KS-AT fragment of DEBS Module 2 without altering any parameter, similar to a previously characterized antibody, 1B2, which primarily recognizes the N-terminal helical docking domain of DEBS Module 3. Crystal structures of AA5 and 1B2 bound to the KS-AT fragment of Module 2 were solved, revealing distinct recognition features of the two antibodies. These findings provide valuable tools and insights for understanding the structure-function relationships of DEBS Module 2, one of the most well-studied modules of an assembly line polyketide synthase (PKS).
Article
Immunology
Joseph E. Levitt, Haley Hedlin, Sophie Duong, Di Lu, Justin Lee, Bryan Bunning, Nadia Elkarra, Benjamin A. Pinsky, Eileen Heffernan, Eric Springman, Richard B. Moss, Hector F. Bonilla, Julie Parsonnet, Roham T. Zamanian, Jamison J. Langguth, Jenna Bollyky, Chaitan Khosla, Mark R. Nicolls, Manisha Desai, Angela J. Rogers
Summary: The study aimed to evaluate the effectiveness of Acebilustat in treating outpatients with COVID-19, and the results showed that the medication did not shorten symptom duration.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Elise Loppinet, Harrison A. Besser, Christina E. E. Lee, Wei Zhang, Bianxiao Cui, Chaitan Khosla
Summary: Protein dysregulation is a common cause of various diseases. Targeted protein degradation is a promising therapeutic approach for proteins without easily druggable pockets or catalytic sites. This study presents the synthesis and validation of heterobifunctional molecules that bind to a target protein via a small molecule ligand and deliver it to the lysosome using a short gluten peptide. The results demonstrate the effective endocytosis and degradation of representative secreted, cell surface, and transmembrane proteins. Optimizing these prototype molecules could lead to pharmacologically relevant LYTAC agents.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Pharmacology & Pharmacy
Harrison A. Besser, Chaitan Khosla
Summary: This review summarizes the pathogenesis of celiac disease, focusing on the interactions between three molecular families and the potential for pharmacological targeting.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shreya Kishore, Chaitan Khosla
Summary: By mining genomic data from the NCBI database, this study presents an updated catalogue of 8799 non-redundant assembly line polyketide synthase clusters across 4083 species, showing a threefold increase in the past 4 years. Furthermore, 95% of the clusters are 'orphan clusters' with no known chemical or biological characterization. These findings suggest that the diversity of assembly line PKSs remains largely unexplored and highlight the potential of genomics-driven approaches for natural product discovery.
Article
Biochemistry & Molecular Biology
Elise Loppinet, Harrison A. Besser, Agnele Sylvia Sewa, Fu-Chen Yang, Bana Jabri, Chaitan Khosla
Summary: Celiac disease is caused by gluten-derived antigens triggering inflammation through presenting to CD4+ T cells. In this study, we propose a mechanism for the deamidation and concentration of gluten peptides in the lysosomes of antigen-presenting cells, which explains the high concentration of gluten peptides required for an inflammatory response in CeD patients. We found that a ternary complex formed between a gluten peptide, transglutaminase-2 (TG2), and a2-macroglobulin, which was endocytosed by LRP-1. The covalent TG2-peptide adduct underwent endolysosomal decoupling, resulting in the expected deamidated epitope. Our findings suggest a pathogenic role for dendritic cells and/or macrophages in CeD and implicate TG2 in the lysosomal clearance of unwanted proteins.
CELL CHEMICAL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
William R. Goodyer, Benjamin M. Beyersdorf, Lauren Duan, Nynke S. van den Berg, Sruthi Mantri, Francisco X. Galdos, Nazan Puluca, Jan W. Buikema, Soah Lee, Darren Salmi, Elise R. Robinson, Stephan Rogalla, Dillon P. Cogan, Chaitan Khosla, Eben L. Rosenthal, Sean M. Wu
Summary: Researchers have successfully developed targeted antibody-dye conjugates for visualizing the cardiac conduction system (CCS) in mice with high sensitivity, specificity, and resolution. They also created a fully human monoclonal antibody fragment that specifically targets the CCS and demonstrated its potential in modulating CCS biology. Furthermore, they identified a suite of additional cell surface markers for molecularly targeting different subcomponents of the CCS.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)