Article
Chemistry, Analytical
Chia-Chen Chang, Chak-Fu Li, Zu-Han Yang, Pin-Yu Lin, Hsiao-Chien Chang, Chih-Wei Yang
Summary: This study presents a simple and sensitive colorimetric assay for the detection of estradiol. The assay utilizes a target-induced recycling assembly of split aptamer fragments, resulting in the formation of a three-way junction-like structure and the enhanced aggregation of gold nanoparticles. The assay demonstrates a low detection limit and the ability to detect estradiol in complex matrices, making it a promising method for estradiol detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Erica Del Grosso, Patrick Irmisch, Serena Gentile, Leonard J. Prins, Ralf Seidel, Francesco Ricci
Summary: This study presents a general approach to achieve dissipative control over toehold-mediated strand-displacement, a widely used reaction in DNA nanotechnology. By re-engineering the classic reaction, the high-energy invader strand is converted into a low-energy waste product, allowing the system to spontaneously return to its original state over time. This method enables unique temporal activation of DNA systems and is reversible and highly controllable.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Yeru Wang, Yu Wang, Su Liu, Wenyu Sun, Manru Zhang, Long Jiang, Minghan Li, Jinghua Yu, Jiadong Huang
Summary: A smart tripedal DNA walker is designed in this research, which can move freely on the electrode with improved walking efficiency, eliminating the drawbacks of high cost and instability, showing potential in OTA detection applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Shuting Li, Longjiao Zhu, Shenghao Lin, Wentao Xu
Summary: Toehold-mediated strand displacement (TMSD) reactions provide a fast, programmable and efficient way for DNA rehybridization. TMSD reactions have been widely used in biochemical sensing and medical diagnosis due to their enzyme-free and isothermal nature. The field faces challenges and has promising prospects.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Wenchan Deng, Jing Yang Xu, Hanyong Peng, Cheng Zhi Huang, X. Chris Le, Hongquan Zhang
Summary: This study reports a toehold-exchange translator and translator-mediated DNAzyme motor systems that enable sensitive responses to various nucleic acid targets using the same DNAzyme motor without requiring redesign. The translator efficiently converts nucleic acid targets into specific output DNA, activating the DNAzyme motor and enabling autonomous movement. By adjusting the target-binding region of the translator, the same DNAzyme motor system can respond to multiple nucleic acid targets. The study also demonstrates the construction and operation of four logic gates using the translator and DNAzyme motor, enhancing the biosensing applications of DNA motors.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Jinbo Zhu, Filip Boskovic, Bao-Nguyen T. Nguyen, Jonathan R. Nitschke, Ulrich F. Keyser
Summary: Introducing an external stimulus (Fe4L4)-L-II tetrahedral cage enables modulation of SDR kinetics. The binding of the cage on the toehold end slows down SDR, while stabilizing intermediates containing two overhangs accelerates the reaction.
Article
Chemistry, Multidisciplinary
Woongsun Choi, Eunhye Park, Seojin Bae, Kyung-Hak Choi, Sangeun Han, Kuk-Hui Son, Do Young Lee, Il-Joo Cho, Hyejeong Seong, Kyo Seon Hwang, Jwa-Min Nam, Jungkyu Choi, Hyojin Lee, Nakwon Choi
Summary: This study presents a novel technology, SWITCH, for PCR-free, multiplex SNP genotyping with a single reporting fluorophore. The technology successfully demonstrated 4-plex detection of warfarin-associated SNP targets with excellent specificity.
Article
Chemistry, Analytical
Shu Zhang, Jiaoyan Yan, Ye Yang, Fei Mo, Yan Li, Hui Huang, Lichao Fang, Jian Huang, Junsong Zheng
Summary: This study developed a facile, rapid, and highly sensitive electrochemical biosensor for the detection and site analysis of DNA methylation. The method showed high sensitivity and rapid response, and did not require complex processing steps. It has the potential to be valuable for early clinical diagnosis and risk assessment.
Article
Biophysics
Shulian Bai, Bangtian Xu, Jiangling Wu, Guoming Xie
Summary: The study introduces the mechanisms of series and parallel toehold-mediated strand displacement for efficient chemical reactions when the target area is obstructed by secondary structures. By adjusting the length of the toeholds, reaction rates can be regulated and multifunctional regulation and selectivity can be achieved. The impressive function of this approach is demonstrated in a logic operation system.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Zhimei Huang, Xianbo Ma, Fengze Jiang, Rong Wang, Zhenkun Wu, Yi Lu
Summary: DNAwalkers have been widely used in biosynthesis, biocomputing, and biosensing due to their programmability, biocompatibility, and signal amplification capacity. A dual spatially localized DNA walker was designed to drive high-speed stochastic movement along three-dimensional tracks using bipedal catalysts. The colocalization of autocatalytic circuits and the use of bipedal catalysts significantly increased the reaction kinetics. An RNA-responsive PCHA walker was constructed for mRNA imaging in live cells, providing a novel and efficient tool for biomolecule detection and biological functions regulation.
Article
Chemistry, Analytical
Zhang Zhang, Zhi Weng, Juan Yao, Dan Liu, Li Zhang, Limei Zhang, Guoming Xie
Summary: Small variations in nucleic acid sequences can have significant impact on diseases. We developed a PCR-based method to construct a high-selectivity long stranded detection probe, which was successfully used for genotyping G6PD deficiency in humans. This method has potential applications in disease diagnosis and treatment selection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Thomas Mayer, Lukas Oesinghaus, Friedrich C. Simmel
Summary: We investigated the impact of random DNA sequences on TMSD circuits and found that only a small subpopulation of strongly interacting strands determined the kinetics. We also compared three techniques to speed up TMSD reactions and found that only the last one can be used without sequence constraints.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biophysics
Dan Bai, Xi Zhou, Wang Luo, Hongyan Yu, Shulian Bai, You Wu, Lin Song, Kena Chen, Yaxing Xie, Xueping Chen, Jie Zhao, Yixin Fu, Yujun Yang, Junjie Li, Guoming Xie
Summary: Nucleic acid nanotechnologies based on toehold-mediated strand displacement have shown great potential for detecting single-nucleotide variations (SNVs). In this study, a cooperative hybridization strategy relying on a dual-toehold and bulge-loop (DT & BL) probe, coupled with the strand displacement catalytic (SDC) cycle, was developed to improve the specificity and reaction rate. The feasibility of this platform was demonstrated by successfully identifying cancer-related genes and detecting clinical samples. This study offers a new perspective for constructing highly specific probes based on dynamic DNA nanotechnology, and provides a promising tool for clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Zhang Zhang, Li Zhang, Youqiang Wang, Juan Yao, Ting Wang, Zhi Weng, Liu Yang, Guoming Xie
Summary: This study introduces an ultrasensitive electrochemical miRNA biosensor based on strand displacement reaction circuits and strand displacement polymerization reaction, demonstrating high sensitivity and discriminatory capability towards let 7a for early cancer screening, providing a new research avenue.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Xiaolei Song, Qin Ding, Yuepu Pu, Juan Zhang, Rongli Sun, Lihong Yin, Wei Wei, Songqin Liu
Summary: A new non-label and enzyme-free fluorescence biosensor for highly specific detection of OTA was developed using TMSD and G-dimer/ThT. The method successfully analyzed OTA in real food samples, offering a new way for OTA and other toxin analysis detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Motoi Oishi, Kazuki Nakatani
Article
Nanoscience & Nanotechnology
Motoi Oishi, Shotaro Juji
Summary: Accelerated DNA hybridization chain reactions (HCRs) using DNA origami as a scaffold have attracted attention in dynamic DNA nanotechnology. In this study, semilocalized and localized HCR systems were constructed using magnetic beads (MBs) as a simple scaffold for enzyme-free miR-21 detection. The DNA density on MBs was found to be crucial for HCR kinetics and limit of detection.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Motoi Oishi