Article
Biochemistry & Molecular Biology
Dahbia Yatoui, Philipp O. Tsvetkov, Romain La Rocca, Viktoriia E. Baksheeva, Diane Allegro, Gilles Breuzard, Geraldine Ferracci, Deborah Byrne, Francois Devred
Summary: Researchers have identified three zinc binding sites on Tau protein and found that zinc binding to these sites plays an important role in aggregation. Particularly, lack of zinc binding to the high affinity site in the repeat region prevents Tau protein from aggregating.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Sandipan Mukherjee, Dulal Panda
Summary: The dysregulation of ferric ions leads to enhanced aggregation of tau protein, with Fe3+ decreasing the critical concentration required for liquid-liquid phase separation of tau and significantly increasing the formation of tau oligomers and fibrils. Fe3+ binds to tau with higher affinity compared to Fe2+ and disrupts the secondary structure of tau, favoring tau aggregation by increasing beta-sheet content and decreasing disordered content. Fe3+ may enhance non-covalent interactions between disordered domains of tau molecules, ultimately leading to tau aggregation and pointing to a potential relationship between dysregulation of ferric ions and neurodegenerative disorders.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Anuradha Venkatramani, Sandipan Mukherjee, Anuradha Kumari, Dulal Panda
Summary: The natural naphthoquinone shikonin can impede the aggregation and fibrillization of tau protein, reducing the size of tau aggregates and inhibiting the formation of tau filaments. It also protects neuroblastoma cells from toxicity induced by tau aggregates. Shikonin shows promise as a potential therapeutic agent for tauopathies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Chengchen Wu, Junyi Zhao, Qiuping Wu, Qiulong Tan, Qiong Liu, Shifeng Xiao
Summary: The study shows that tau protein can undergo LLPS in neurons, forming different types of condensates in cells based on the N-terminal inserts of tau isoforms. The absence of N-terminal inserts significantly affects the size and functionality of tau condensates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Arpan Pradhan, Satyendra Mishra, Avadhesha Surolia, Dulal Panda
Summary: C1, a synthetic derivative of curcumin, strongly inhibits the aggregation and filament formation of tau, protects neuroblastoma cells from the deleterious effects of tau oligomers, and reduces the mean diameter and quantity of tau oligomers.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ying-Ying Gao, Tao Zhong, Li-Qiang Wang, Na Zhang, Yan Zeng, Ji-Ying Hu, Hai-Bin Dang, Jie Chen, Yi Liang
Summary: Zinc promotes liquid-liquid phase separation and aggregation of full-length Tau, resulting in the formation of stress granules and interaction with G3BP1, leading to increased Tau filaments and neuronal toxicity. Additionally, zinc aggravates mitochondrial damage and reactive oxygen species production induced by Tau aggregation, further deteriorating the pathogenesis of Alzheimer's disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Yanxian Lin, Yann Fichou, Andrew P. Longhini, Luana C. Llanes, Pengyi Yin, Guillermo C. Bazan, Kenneth S. Kosik, Songi Han
Summary: The liquid-liquid phase separation of tau protein can facilitate amyloid aggregation in some cases, while being independent of aggregation in others. The nature of the interaction driving tau condensation is a differentiating factor between aggregation-prone and aggregation-independent liquid-liquid phase separations.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guangfei Duan, Yanqin Li, Meimei Ye, Hexin Liu, Ning Wang, Shizhong Luo
Summary: Transthyretin (TTR) aggregation and amyloid formation in ATTR diseases involve liquid-liquid phase separation (LLPS) and subsequent liquid-to-solid phase transition. Electrostatic interactions mediate LLPS of TTR, and pathogenic mutations and heparin promote the process of phase transition and aggregate formation. Post-translational modifications of TTR, such as S-cysteinylation and S-sulfonation, affect the stability and aggregation propensity of TTR. These findings provide molecular insights into the mechanism of TTR aggregation and suggest potential therapeutic targets for ATTR diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Xuewei Dong, Santu Bera, Qin Qiao, Yiming Tang, Zenghui Lao, Yin Luo, Ehud Gazit, Guanghong Wei
Summary: Liquid-liquid phase separation (LLPS) plays a role in physiological and pathological processes. Tau and its K18 construct undergo LLPS in a temperature-dependent manner, but the underlying mechanism remains largely elusive.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Katarzyna Soltys, Aneta Tarczewska, Dominika Bystranowska
Summary: Liquid-liquid phase separation is an important phenomenon in the cellular organization of biomolecules, leading to the formation of membraneless organelles. Membraneless organelles create concentrated microenvironments for specific biomolecules. Dysfunctional membraneless organelles can be associated with diseases. Therefore, identifying factors that regulate liquid-liquid phase separation is of great interest.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Roberto Tira, Giovanna Viola, Carlo Giorgio Barracchia, Francesca Parolini, Francesca Munari, Stefano Capaldi, Michael Assfalg, Mariapina D'Onofrio
Summary: Espresso coffee has a protective effect against neurodegenerative disorders such as Alzheimer's disease. The coffee extract contains caffeine and genistein, which prevent the aggregation and fibrillization of tau protein. Additionally, certain coffee compounds can bind to preformed tau fibrils.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Summary: Phosphorylation of tau at specific epitopes can affect its ability to nucleate microtubule bundles during LLPS, while phosphorylation at other sites does not inhibit tubulin assembly from tau droplets. This suggests that IDP phosphorylation may be a widely used mechanism for regulating condensate-mediated cytoskeletal assembly.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Cell Biology
Fei Yi, Chunmiao Cai, Banzhan Ruan, Mingang Hao, Syn Kok Yeo, Michael Haas, Fuchun Yang, Xiaoting Zhang, Jun-Lin Guan
Summary: RB1CC1/FIP200 is an important autophagy protein that plays a crucial role in various biological processes and diseases. This study reveals a new post-translational mechanism of RB1CC1 regulation through acetylation. Additionally, RB1CC1 contains an intrinsically disordered region capable of liquid-liquid phase separation, leading to the formation of puncta independent of other autophagy receptors.
Article
Multidisciplinary Sciences
Vikas A. Tillu, James Rae, Ya Gao, Nicholas Ariotti, Matthias Floetenmeyer, Oleksiy Kovtun, Kerrie-Ann Mcmahon, Natasha Chaudhary, Robert G. Parton, Brett M. Collins
Summary: The study highlights the essential role of the three disordered domains of Cavin1 in caveola formation and dynamic trafficking, as well as the fuzzy electrostatic interactions between Cavin1 and caveolin-1 proteins, combined with membrane lipid interactions, in generating membrane curvature and maintaining a stable caveola coat.
NATURE COMMUNICATIONS
(2021)
Review
Neurosciences
Hassan Ainani, Najat Bouchmaa, Reda Ben Mrid, Rachid El Fatimy
Summary: Metabolic reactions within cells occur in various isolated compartments with or without borders, the latter being known as membrane-less organelles (MLOs). The MLOs show liquid-like properties and are formed by a process known as liquid-liquid phase separation (LLPS). MLOs contribute to different molecules interactions such as protein-protein, protein-RNA, and RNA-RNA driven by various factors, such as multivalency of intrinsic disor-ders. MLOs are involved in several cell signaling pathways such as transcription, immune response, and cellular organization. However, disruption of these processes has been found in different pathologies.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Pharmacology & Pharmacy
Vivian W. Y. Liao, Anuradha Kumari, Rajeshwar Narlawar, Soma Vignarajan, David E. Hibbs, Dulal Panda, Paul W. Groundwater
MOLECULAR PHARMACOLOGY
(2020)
Editorial Material
Cell Biology
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Biochemistry & Molecular Biology
Anuradha Kumari, Omprakash Shriwas, Shailendra Sisodiya, Manas K. Santra, Sankar K. Guchhait, Rupesh Dash, Dulal Panda
Summary: The study demonstrates that microtubule-targeting agents inhibit the signaling of beta-catenin in oral squamous cell carcinoma, leading to a decrease in cancer malignancy. Additionally, the combined use of these agents with Wnt/beta-catenin signaling antagonists shows promise as a strategy for cancer chemotherapy.
Article
Environmental Sciences
Manish Kumar, Anuradha Kumari, Deepak Kumar, Nadhir Al-Ansari, Rawshan Ali, Raushan Kumar, Ambrish Kumar, Ahmed Elbeltagi, Alban Kuriqi
Summary: This study evaluated the estimation of pan evaporation using different input parameters and various models. The SVM-RF model outperformed others in all scenarios, providing better decision-making support for water managers and planners.
Article
Biochemistry & Molecular Biology
Anuradha Venkatramani, Sandipan Mukherjee, Anuradha Kumari, Dulal Panda
Summary: The natural naphthoquinone shikonin can impede the aggregation and fibrillization of tau protein, reducing the size of tau aggregates and inhibiting the formation of tau filaments. It also protects neuroblastoma cells from toxicity induced by tau aggregates. Shikonin shows promise as a potential therapeutic agent for tauopathies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Civil
Manish Kumar, Ahmed Elbeltagi, Chaitanya B. Pande, Ali Najah Ahmed, Ming Fai Chow, Quoc Bao Pham, Anuradha Kumari, Deepak Kumar
Summary: Accurate and reliable discharge estimation is vital in managing water resources, agriculture, industry, and flood management. This study compares the performance of five data-driven tree-based algorithms in measuring the daily discharge of Govindpur site at Burhabalang river, India. The results show that the Random Tree model (Model-3) outperforms other models and can be used as a robust model for sustainable flood plain management.
WATER RESOURCES MANAGEMENT
(2022)
Article
Environmental Sciences
Anuradha Kumari, Akhilesh Kumar, Manish Kumar, Alban Kuriqi
Summary: This study models grain velocity using soft computing approaches and evaluates the performance using quantitative indices. The results show that the SVM model provides more accurate predictions during the testing phase.
Article
Computer Science, Artificial Intelligence
M. A. Ganaie, Anuradha Kumari, A. K. Malik, M. Tanveer
Summary: This paper discusses the application of support vector machines (SVMs) in diagnosing neurological disorders and the challenges of dealing with noise and outliers in EEG signal classification. The authors propose an improved intuitionistic fuzzy twin support vector machine (IIFTWSVM) to address these challenges and evaluate its performance compared to baseline models.
NEURAL COMPUTING & APPLICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Anuradha Kumari, Shweta S. Prassanawar, Dulal Panda
Summary: Indibulin, a microtubule-depolymerizing agent, shows minimal neurotoxicity and is less cytotoxic towards differentiated neuronal cells. It binds to beta-tubulin isotypes differently and has a lower affinity to beta-III tubulin. Depletion of beta-III tubulin increases the toxicity of indibulin and colchicine, while vinblastine is unaffected. Indibulin inhibits colchicine binding to tubulin and is a colchicine-site binder.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Pooja J. Batra, Anuradha Kumari, Vivian W. Y. Liao, David E. Hibbs, Paul W. Groundwater, Dulal Panda
Summary: In this study, it was found that 3,5-bis[(1E)-2-(2,6-dichlorophenyl)ethenyl]-1H-pyrazole 2l depolymerizes microtubules and reduces their growing tips. Pyrazole 2l treatment inhibits chromosome movements, activates the spindle assembly checkpoint, and blocks mitosis in MCF-7 cells. Furthermore, it induces cell death through various pathways and exhibits stronger antiproliferative effects in cancerous cells compared to non-cancerous cells.
JOURNAL OF BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Pritam Giri, Pooja J. Batra, Anuradha Kumari, Neha Hura, Rishav Adhikary, Ayan Acharya, Sankar Kumar Guchhait, Dulal Panda
Summary: This study explored the creation of new molecules with anticancer potential from clinical trial agents. A compound named QTMP showed stronger antiproliferative ability than the clinical trial agent CA-4 in various cancer cell lines, and it also affected microtubule assembly and cell cycle progression. In vitro analysis indicated that QTMP possesses favorable drug-like properties.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Meeting Abstract
Biophysics
Anuradha Venkatramani, Sandipan Mukherjee, Anuradha Kumari, Dulal Panda
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Jitendra S. Rane, Anuradha Kumari, Dulal Panda
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Shweta Shyam Prassanawar, Anuradha Kumari, Sankar K. Guchhait, Dulal Panda