Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop

Multi-omic measurements of heterogeneity in HeLa cells across laboratories

(2019) Yansheng Liu et al.   NATURE BIOTECHNOLOGY

Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses

(2019) Fanghui Zhang et al.   IMMUNITY

Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo

(2019) Frederik J. Verweij et al.   DEVELOPMENTAL CELL

Emerging roles of extracellular vesicles in neurodegenerative disorders

(2019) Yang You et al.   NEUROBIOLOGY OF DISEASE

Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures

(2019) Katharina B. Beer et al.   Nature Communications

Uptake Profiles of Human Serum Exosomes by Murine and Human Tumor Cells through Combined Use of Colloidal Nanoplasmonics and Flow Cytofluorimetric Analysis

(2018) Sara Busatto et al.   ANALYTICAL CHEMISTRY

PKH26 labeling of extracellular vesicles: Characterization and cellular internalization of contaminating PKH26 nanoparticles

(2018) Pia Pužar Dominkuš et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Retrovirus-like Gag Protein Arc1 Binds RNA and Traffics across Synaptic Boutons

(2018) James Ashley et al.   CELL

Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

(2018) Marco P. Monopoli et al.   CHEMICAL COMMUNICATIONS

Stimulation of exosome release by extracellular DNA is conserved across multiple cell types

(2018) Dimitar Iliev et al.   FEBS Journal

Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling

(2018) Frederik Johannes Verweij et al.   JOURNAL OF CELL BIOLOGY

Terminology matters: There is no targeting, but retention

(2018) Joshua Reineke   JOURNAL OF CONTROLLED RELEASE

Flow cytometric analysis of extracellular vesicle subsets in plasma: impact of swarm by particles of non-interest

(2018) S. F. W. M. Libregts et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Technical challenges of working with extracellular vesicles

(2018) Marcel I. Ramirez et al.   Nanoscale

Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

(2018) Haiying Zhang et al.   NATURE CELL BIOLOGY

Shedding light on the cell biology of extracellular vesicles

(2018) Guillaume van Niel et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Extracellular vesicle budding is inhibited by redundant regulators of TAT-5 flippase localization and phospholipid asymmetry

(2018) Katharina B. Beer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exosomal lipid composition and the role of ether lipids and phosphoinositides in exosome biology

(2018) Tore Skotland et al.   JOURNAL OF LIPID RESEARCH

Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach

(2018) Sander A. A. Kooijmans et al.   Nanoscale

Rab35-dependent extracellular nanovesicles are required for induction of tumour supporting stroma

(2018) V. Yeung et al.   Nanoscale

Arrestin-Domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles

(2018) Sushma Anand et al.   PROTEOMICS

Analytical Considerations in Nanoscale Flow Cytometry of Extracellular Vesicles to Achieve Data Linearity

(2018) Janice Gomes et al.   THROMBOSIS AND HAEMOSTASIS

Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication

(2018) Mathilde Mathieu et al.   NATURE CELL BIOLOGY

Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs

(2018) Albert Lu et al.   eLife

Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis

(2017) Helena Costa Verdera et al.   JOURNAL OF CONTROLLED RELEASE

Liposome encapsulated berberine treatment attenuates cardiac dysfunction after myocardial infarction

(2017) Iris E. Allijn et al.   JOURNAL OF CONTROLLED RELEASE

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

(2017) Jose C. Contreras-Naranjo et al.   LAB ON A CHIP

Fluorescence labelling of extracellular vesicles using a novel thiol-based strategy for quantitative analysis of cellular delivery and intracellular traffic

(2017) H. D. Roberts-Dalton et al.   Nanoscale

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

(2017) Sushrut Kamerkar et al.   NATURE

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

(2017) Jan Van Deun et al.   NATURE METHODS

Why the need and how to approach the functional diversity of extracellular vesicles

(2017) Mercedes Tkach et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Detection of platelet vesicles by flow cytometry

(2017) John P Nolan et al.   PLATELETS

Coding and noncoding landscape of extracellular RNA released by human glioma stem cells

(2017) Zhiyun Wei et al.   Nature Communications

Plasma membrane-derived extracellular microvesicles mediate non-canonical intercellular NOTCH signaling

(2017) Qiyu Wang et al.   Nature Communications

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23

(2017) Yao Wei et al.   Nature Communications

Detection of phosphatidylserine-positive exosomes as a diagnostic marker for ovarian malignancies: a proof of concept study

(2017) Jayanthi Lea et al.   Oncotarget

TLR-exosomes exhibit distinct kinetics and effector function

(2017) Swetha Srinivasan et al.   Scientific Reports

Imaging and Intracellular Tracking of Cancer-Derived Exosomes Using Single-Molecule Localization-Based Super-Resolution Microscope

(2016) Chen Chen et al.   ACS Applied Materials & Interfaces

A new approach to follow a single extracellular vesicle–cell interaction using optical tweezers

(2016) Ilaria Prada et al.   BIOTECHNIQUES

Exosomes induce and reverse monocrotaline-induced pulmonary hypertension in mice

(2016) Jason M. Aliotta et al.   CARDIOVASCULAR RESEARCH

Introduction to Extracellular Vesicles: Biogenesis, RNA Cargo Selection, Content, Release, and Uptake

(2016) Erik R. Abels et al.   CELLULAR AND MOLECULAR NEUROBIOLOGY

Identification of a novel mechanism of blood–brain communication during peripheral inflammation via choroid plexus‐derived extracellular vesicles

(2016) Sriram Balusu et al.   EMBO Molecular Medicine

Reversible HuR‐microRNA binding controls extracellular export of miR‐122 and augments stress response

(2016) Kamalika Mukherjee et al.   EMBO REPORTS

Cortactin promotes exosome secretion by controlling branched actin dynamics

(2016) Seema Sinha et al.   JOURNAL OF CELL BIOLOGY

Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER

(2016) Wolf Heusermann et al.   JOURNAL OF CELL BIOLOGY

PEGylated and targeted extracellular vesicles display enhanced cell specificity and circulation time

(2016) S.A.A. Kooijmans et al.   JOURNAL OF CONTROLLED RELEASE

Mast Cell Degranulation Is Accompanied by the Release of a Selective Subset of Extracellular Vesicles That Contain Mast Cell–Specific Proteases

(2016) Tom Groot Kormelink et al.   JOURNAL OF IMMUNOLOGY

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes

(2016) Joanna Kowal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanisms and functions of extracellular vesicle release in vivo—What we can learn from flies and worms

(2016) Katharina B. Beer et al.   Cell Adhesion & Migration

Fetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA

(2016) Zhiyun Wei et al.   Scientific Reports

KRAS-MEK Signaling Controls Ago2 Sorting into Exosomes

(2016) Andrew J. McKenzie et al.   Cell Reports

Tetherin is an exosomal tether

(2016) James R Edgar et al.   eLife

Regulation of the divalent metal ion transporter via membrane budding

(2016) KimberlyD Mackenzie et al.   Cell Discovery

Distribution of Systemically Administered Nanoparticles Reveals a Size-Dependent Effect Immediately following Cardiac Ischaemia-Reperfusion Injury

(2016) David J. Lundy et al.   Scientific Reports

Differential and transferable modulatory effects of mesenchymal stromal cell-derived extracellular vesicles on T, B and NK cell functions

(2016) Mariano Di Trapani et al.   Scientific Reports

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis

(2016) Neda Najafinobar et al.   Scientific Reports

Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation

(2016) Aleksander Cvjetkovic et al.   Scientific Reports

Colorimetric Nanoplasmonic Assay To Determine Purity and Titrate Extracellular Vesicles

(2015) Daniele Maiolo et al.   ANALYTICAL CHEMISTRY

The influence of cholesterol on membrane protein structure, function, and dynamics studied by molecular dynamics simulations

(2015) Julie Grouleff et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high-resolution flow cytometry

(2015) Tom Groot Kormelink et al.   CYTOMETRY PART A

Active endocannabinoids are secreted on extracellular membrane vesicles

(2015) M. Gabrielli et al.   EMBO REPORTS

Mechanisms of RNA loading into exosomes

(2015) Teresa Janas et al.   FEBS LETTERS

Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions

(2015) Anurag Purushothaman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

RAL-1 controls multivesicular body biogenesis and exosome secretion

(2015) Vincent Hyenne et al.   JOURNAL OF CELL BIOLOGY

Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics

(2015) Sybren L.N. Maas et al.   JOURNAL OF CONTROLLED RELEASE

Tumour exosome integrins determine organotropic metastasis

(2015) Ayuko Hoshino et al.   NATURE

Glypican-1 identifies cancer exosomes and detects early pancreatic cancer

(2015) Sonia A. Melo et al.   NATURE

Studying extracellular vesicle transfer by a Cre-loxP method

(2015) Anoek Zomer et al.   Nature Protocols

Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties

(2015) Xabier Osteikoetxea et al.   PLoS One

Ectosomes and exosomes: shedding the confusion between extracellular vesicles

(2015) Emanuele Cocucci et al.   TRENDS IN CELL BIOLOGY

Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters

(2015) Charles P. Lai et al.   Nature Communications

Dynamic Biodistribution of Extracellular Vesicles in Vivo Using a Multimodal Imaging Reporter

(2014) Charles P. Lai et al.   ACS Nano

Biophysical investigations with MARCKS-ED: dissecting the molecular mechanism of its curvature sensing behaviors

(2014) Leslie A. Morton et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

(2014) Marvin E. Tanenbaum et al.   CELL

Regulation of exosome release by glycosphingolipids and flotillins

(2014) Santosh Phuyal et al.   FEBS Journal

Serotonin stimulates secretion of exosomes from microglia cells

(2014) Konstantin Glebov et al.   GLIA

Biodistribution of mesenchymal stem cell-derived extracellular vesicles in a model of acute kidney injury monitored by optical imaging

(2014) CRISTINA GRANGE et al.   INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

Extracellular vesicles as drug delivery systems: Lessons from the liposome field

(2014) Roy van der Meel et al.   JOURNAL OF CONTROLLED RELEASE

Induction of Exosome Release in Primary B Cells Stimulated via CD40 and the IL-4 Receptor

(2014) S. C. Saunderson et al.   JOURNAL OF IMMUNOLOGY

Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing

(2014) E. van der Pol et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Magnetic resonance imaging of melanoma exosomes in lymph nodes

(2014) Lingzhi Hu et al.   MAGNETIC RESONANCE IN MEDICINE

Emerging role of extracellular vesicles in inflammatory diseases

(2014) Edit I. Buzas et al.   Nature Reviews Rheumatology

Circulating Extracellular Vesicles with Specific Proteome and Liver MicroRNAs Are Potential Biomarkers for Liver Injury in Experimental Fatty Liver Disease

(2014) Davide Povero et al.   PLoS One

Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis

(2014) T. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exosome Uptake Depends on ERK1/2-Heat Shock Protein 27 Signaling and Lipid Raft-mediated Endocytosis Negatively Regulated by Caveolin-1

(2013) Katrin J. Svensson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Intracellular Interactome of Tetraspanin-enriched Microdomains Reveals Their Function as Sorting Machineries toward Exosomes

(2013) Daniel Perez-Hernandez et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Extracellular vesicles: Exosomes, microvesicles, and friends

(2013) Graça Raposo et al.   JOURNAL OF CELL BIOLOGY

Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles

(2013) Marina Colombo et al.   JOURNAL OF CELL SCIENCE

Sensitivity enhancement and contrasting information provided by free radicals in oriented-sample NMR of bicelle-reconstituted membrane proteins

(2013) Deanna M. Tesch et al.   JOURNAL OF MAGNETIC RESONANCE

Regulation of Postsynaptic Retrograde Signaling by Presynaptic Exosome Release

(2013) Ceren Korkut et al.   NEURON

Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs

(2013) Carolina Villarroya-Beltri et al.   Nature Communications

Exosome Secretion Is Enhanced by Invadopodia and Drives Invasive Behavior

(2013) Daisuke Hoshino et al.   Cell Reports

MARCKS-ED Peptide as a Curvature and Lipid Sensor

(2012) Leslie A. Morton et al.   ACS Chemical Biology

Exosomes as nano-theranostic delivery platforms for gene therapy

(2012) Aaron Tan et al.   ADVANCED DRUG DELIVERY REVIEWS

Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy

(2012) Y. Lee et al.   HUMAN MOLECULAR GENETICS

Active Wnt proteins are secreted on exosomes

(2012) Julia Christina Gross et al.   NATURE CELL BIOLOGY

Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

(2012) Héctor Peinado et al.   NATURE MEDICINE

Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry

(2012) Els J van der Vlist et al.   Nature Protocols

A Knockout of the Tsg101 Gene Leads to Decreased Expression of ErbB Receptor Tyrosine Kinases and Induction of Autophagy Prior to Cell Death

(2012) Chantey R. Morris et al.   PLoS One

Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein

(2012) J. F. Nabhan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanodiscs versus Macrodiscs for NMR of Membrane Proteins

(2011) Sang Ho Park et al.   BIOCHEMISTRY

Blood Cell Origin of Circulating MicroRNAs: A Cautionary Note for Cancer Biomarker Studies

(2011) C. C. Pritchard et al.   Cancer Prevention Research

Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes

(2011) R Alonso et al.   CELL DEATH AND DIFFERENTIATION

Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles

(2011) Bence György et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

The P4-ATPase TAT-5 Inhibits the Budding of Extracellular Vesicles in C. elegans Embryos

(2011) Ann M. Wehman et al.   CURRENT BIOLOGY

Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC

(2011) Yanyan Li et al.   ELECTROPHORESIS

Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer

(2011) Raymond S Lance et al.   Expert Review of Anticancer Therapy

Lipid Raft Endocytosis and Exosomal Transport Facilitate Extracellular Trafficking of Annexin A2

(2011) Mallika Valapala et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Haemolysis during Sample Preparation Alters microRNA Content of Plasma

(2011) Michaela B. Kirschner et al.   PLoS One

Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A–C

(2010) Chieh Hsu et al.   JOURNAL OF CELL BIOLOGY

Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers

(2010) Shidong Chu et al.   JOURNAL OF MAGNETIC RESONANCE

Cellular Internalization of Exosomes Occurs Through Phagocytosis

(2010) Du Feng et al.   TRAFFIC

Roles of Cholesterol in Vesicle Fusion and Motion

(2009) Jing Zhang et al.   BIOPHYSICAL JOURNAL

Exosomal MicroRNA: A Diagnostic Marker for Lung Cancer

(2009) Guilherme Rabinowits et al.   Clinical Lung Cancer

Acid sphingomyelinase activity triggers microparticle release from glial cells

(2009) Fabio Bianco et al.   EMBO JOURNAL

Microenvironmental pH Is a Key Factor for Exosome Traffic in Tumor Cells

(2009) Isabella Parolini et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Solution NMR Approach to the Measurement of Amphiphile Immersion Depth and Orientation in Membrane Model Systems

(2009) M. Sameer Al-Abdul-Wahid et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity

(2009) Derrick J. Gibbings et al.   NATURE CELL BIOLOGY

Rab27a and Rab27b control different steps of the exosome secretion pathway

(2009) Matias Ostrowski et al.   NATURE CELL BIOLOGY

High Levels of Exosomes Expressing CD63 and Caveolin-1 in Plasma of Melanoma Patients

(2009) Mariantonia Logozzi et al.   PLoS One

Activated T cells recruit exosomes secreted by dendritic cells via LFA-1

(2008) E. N. M. Nolte-'t Hoen et al.   BLOOD

Viral membrane fusion

(2008) Stephen C Harrison   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes

(2008) K. Trajkovic et al.   SCIENCE

Calculation of Free Energy Barriers to the Fusion of Small Vesicles

(2007) J.Y. Lee et al.   BIOPHYSICAL JOURNAL