期刊
CHEMICAL COMMUNICATIONS
卷 54, 期 52, 页码 7219-7222出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc02135j
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资金
- Science Foundation Ireland [11/PI/1071(T)]
- Science Foundation Ireland (SFI) [11/PI/1071(T)] Funding Source: Science Foundation Ireland (SFI)
The recognition of the biological, diagnostic andmedical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.
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