期刊
CELLS
卷 8, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/cells8111367
关键词
Chlamydomonas; growth; acetate concentration; transcriptomics; surprisal analysis
类别
资金
- FP7-Future and Emerging Technologies-Open Project (BAMBI project) [618024]
- Fonds National de la Recherche Scientifique (FRS-FNRS, Belgium) [CDR J.0265.17]
- ALGACTIVE Project (KONNECT-Strengthening STI Cooperation between the EU and Korea, Promoting Innovation and the Enhancement of Communication for Technology-Related Policy Dialogue)
- DARKMET project (Action de Recherche Concertee ARC grant) [17/21-08]
- Fonds Wetenschappelijk Onderzoek-Vlaanderen (FWO)
- Fonds de la Recherche Scientifique-FNRS under EOS [30829584]
- FRS-FNRS, Belgium
Acetate can be efficiently metabolized by the green microalga Chlamydomonasreinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were cultivated in batch mode in the light at 17, 31, 44, and 57 mM acetate. Metabolic analyses show that cells grown at 57 mM acetate possess increased contents of all components analyzed (starch, chlorophylls, fatty acids, and proteins), with a three-fold increased volumetric biomass yield compared to cells cultivated at 17 mM acetate at the entry of stationary phase. Physiological analyses highlight the importance of photosynthesis for the low-acetate and exponential-phase samples. The stationary phase is reached when acetate is depleted, except for the cells grown at 57 mM acetate, which still divide until ammonium exhaustion. Surprisal analysis of the transcriptomics data supports the biological significance of our experiments. This allows the establishment of a model for acetate assimilation, its transcriptional regulation and the identification of candidates for genetic engineering of this metabolic pathway. Altogether, our analyses suggest that growing at high-acetate concentrations could increase biomass productivities in low-light and CO2-limiting air-bubbled medium for biotechnology.
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