Article
Food Science & Technology
P. Chang, B. Tai, M. Zheng, Q. Yang, F. Xing
Summary: Aspergillus flavus is a pathogenic fungus that causes significant crop losses and has negative effects on human and animal health. This study found that natamycin, extracted from Streptomyces spp., can effectively inhibit fungal growth and conidia germination, as well as reduce the production of ergosterol and AFB 1 by A. flavus. The inhibitory effects of natamycin on fungal growth and AFB 1 biosynthesis were achieved by down-regulating genes involved in ergosterol biosynthesis and conidia development. The findings provide solid evidence for the application of natamycin in controlling AFB 1 contamination.
WORLD MYCOTOXIN JOURNAL
(2022)
Article
Microbiology
Liuke Liang, Wei Zhang, Jing Hao, Yanyu Wang, Shan Wei, Shuaibing Zhang, Yuansen Hu, Yangyong Lv
Summary: This study found that estragole from Pimenta racemosa has antifungal activity against Aspergillus flavus and inhibits its growth and aflatoxin biosynthesis by modulating intracellular redox homeostasis. These findings provide a basis for estragole as a potential agent against A. flavus contamination.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Wei Zhang, Bangbang Li, Yangyong Lv, Shan Wei, Shuaibing Zhang, Yuansen Hu
Summary: In this study, honokiol, a phenolic compound in Magnolia officinalis, was found to inhibit the germination and growth of Aspergillus flavus. It also showed potent antifungal activity against A. flavus in corn flour by suppressing conidia production. The study explored the mechanisms of honokiol's inhibition on A. flavus, including increased plasma membrane permeability, inhibition of ATPase activity, mitochondrial dysfunction, and accumulation of reactive oxygen species. Transcriptomic analysis revealed that honokiol treatment resulted in differentially expressed genes related to spore development, cell wall and membrane integrity, oxidative stress, and energy metabolism. These findings enhance the understanding of honokiol's antifungal activity and its potential as an agent in preventing contamination by A. flavus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Review
Immunology
Jingni Wu, Zhixiao Fang, Teng Liu, Wei Hu, Yangjun Wu, Shengli Li
Summary: Inflammatory skin diseases are caused by disorders in the skin's defense system, resulting in recurrent skin lesions and intense itching that impact life quality. Transcriptomic profiling helps uncover molecular factors of typical inflammatory skin diseases, offering guidance for improved diagnosis and treatment strategies. High-throughput transcriptomics data provides unprecedented insights into the complex pathophysiology of these diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jun Seo Ha, Hyundoo Jeong
Summary: Recent advances in single-cell sequencing techniques have allowed for gene expression profiling at the individual cell level, accelerating biomedical research for complex disease treatment. In this study, we introduce a novel single-cell clustering algorithm, GRACE, which employs ensemble similarity learning and graph autoencoders to accurately classify cells. Through evaluations using real-world single-cell sequencing datasets, we demonstrate that GRACE achieves high assessment metric scores and yields accurate single-cell clustering results.
Article
Plant Sciences
Amanda Bullones, Antonio Jesus Castro, Elena Lima-Cabello, Noe Fernandez-Pozo, Rocio Bautista, Juan de Dios Alche, Manuel Gonzalo Claros
Summary: This study used RNA-seq technology to investigate the genome-wide events during the in vitro germination of olive pollen. The results revealed a deep transcriptional reprogramming in mature olive pollen and identified several important genes related to heat shock proteins and F-box proteins. Additionally, the study found that olive pollen is equipped with transcripts to cope with adverse environments, but in vitro germination induced multiple stress responses. The research also discovered an overall increased number of pollen-specific gene isoforms in olive trees compared to other plants.
Article
Biotechnology & Applied Microbiology
Sheng-Fa Li, Shuai-Bing Zhang, Yang-Yong Lv, Huan-Chen Zhai, Yuan-Sen Hu, Jing-Ping Cai
Summary: This study reveals the inhibitory mechanism of heptanal against A. flavus by changing the surface morphology of A. flavus spores and affecting the expression of genes related to cell wall and plasma damage, energy metabolism, RNA and DNA synthesis. Heptanal can also induce early apoptosis of A. flavus spores.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Liang Qin, Shuai-Bing Zhang, Yang-Yong Lv, Huan-Chen Zhai, Yuan-Sen Hu, Jing-Ping Cai
Summary: This study provides new insights into the antifungal mechanisms of 1-nonanol against A. flavus, showing that 1-nonanol can distort spore morphology, induce increased membrane permeability, damage DNA, and exert destructive effects on A. flavus spores.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Joseph D. Romano, Hai Li, Tanya Napolitano, Ronald Realubit, Charles Karan, Mande Holford, Nicholas P. Tatonetti
Summary: VenomSeq is a new platform that systematically identifies associations between venoms and drugs/diseases using high-throughput transcriptomics and perturbational differential gene expression analysis. By integrating comparisons to public repositories of differential expression, associations between regulatory networks and disease, and existing knowledge of venom activity, it provides new therapeutic hypotheses linking venoms to human diseases supported by multiple layers of preliminary evidence.
Article
Biotechnology & Applied Microbiology
Sheng-Fa Li, Shuai-Bing Zhang, Huan-Chen Zhai, Yang-Yong Lv, Yuan-Sen Hu, Jing-Ping Cai
Summary: Hexanal, a natural compound, disrupts cell membrane and mitochondrial function in Aspergillus flavus, leading to apoptosis. This study reveals that hexanal inhibits A. flavus growth by increasing reactive oxygen species, reducing mitochondrial membrane potential, and causing DNA fragmentation. Transcriptional analysis shows that hexanal alters the metabolism of A. flavus spores, providing insights into its antifungal mechanism.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Yinhui Jiang, Xiang Liu, Xun Tian, Jianhong Zhou, Qinrong Wang, Bi Wang, Wenfeng Yu, Yanping Jiang, Tom Hsiang, Xiaolan Qi
Summary: RNA interference (RNAi) plays an essential role in the defense against viral infection in fungi. In this study, the antiviral responses of RNAi components were investigated in Aspergillus flavus infected with Aspergillus flavus partitivirus 1 (AfPV1). The expression of RNAi components was induced upon AfPV1 infection, and knock mutants of these components showed reduced production of AfPV1 and increased sporulation. Different RNAi mutants also exhibited varying sensitivity to different stresses. Small RNA sequencing analysis revealed a decrease in the number of unique reads of sRNA in A. flavus upon AfPV1 infection. Functional analysis showed that the affected sRNA functions were related to vacuole production. Overall, this study sheds light on the functional role of RNAi in the hypovirulence of A. flavus caused by AfPV1 infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Sifan Jia, Chong Li, Kuntan Wu, Desheng Qi, Shuai Wang
Summary: In this study, the mechanism of Aspergillus flavus conidia germination inhibition by decreased water activity was explored. The impact of low water activity on cell shape and size was analyzed. Transcriptomics and proteomics identified differentially expressed genes and proteins, with dominant pathways involved in nutrient metabolism and translation. Low water activity was found to inhibit conidia germination and influence the expression of genes related to carbohydrate and amino acid metabolism, as well as translation in Aspergillus flavus.
Article
Biotechnology & Applied Microbiology
Yan-Nan Li, Shuai-Bing Zhang, Yang-Yong Lv, Huan-Chen Zhai, Jing-Ping Cai, Yuan-Sen Hu
Summary: The study found that linalool can effectively inhibit the growth of Aspergillus flavus in stored grains by damaging the cell membrane, causing mitochondrial dysfunction, DNA damage, and inducing autophagy.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Zhenxiao Sun, Yi Zhang, Xinping Lin, Sufang Zhang, Yingxi Chen, Chaofan Ji
Summary: This study analyzed the inhibition pattern and mechanism of Lpb. plantarum on M. morganii, as well as the mechanisms behind changes in different biogenic amines. The results showed that Lpb. plantarum could significantly inhibit the biogenic amines of M. morganii in a weak acid environment, primarily through metabolism. This is important for inhibiting the accumulation of harmful metabolites in fermented foods.
Review
Plant Sciences
Ce Chen, Yining Ge, Lingli Lu
Summary: Single-cell and spatial transcriptomics have shifted researchers' focus from the multicellular level to the single-cell level and spatial information. Single-cell transcriptomes provide insights into transcriptomes at the single-cell level, while spatial transcriptomes help preserve spatial information. Though these two omics technologies are mature and helpful, further research is needed to ensure their widespread applicability in plant studies.
FRONTIERS IN PLANT SCIENCE
(2023)
