Review
Biotechnology & Applied Microbiology
Calef Sanchez-Trasvina, Miguel Flores-Gatica, Daniela Enriquez-Ochoa, Marco Rito-Palomares, Karla Mayolo-Deloisa
Summary: Proteins with biorecognition properties are widely used as therapeutic agents, and can be modified to improve pharmacokinetic properties and stability. Purification steps are crucial for ensuring the quality and safety of the final product, with chromatographic methods being the gold standard for downstream processing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Chemical
Xing Yang, Andrea Merenda, Riyadh AL-Attabi, Ludovic F. Dumee, Xiwang Zhang, San H. Thang, Hung Pham, Lingxue Kong
Summary: Membrane chromatography is considered a potential solution for downstream processing in protein purification. Despite limited commercial adoption in large-scale production, significant progress has been made. However, robust evaluation of its performance is still needed, and the disconnection between academic and commercial efforts should be addressed.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Engineering, Chemical
A. A. M. Abusultan, J. A. Wood, T. Sainio, A. J. B. Kemperman, W. G. J. van der Meer
Summary: Reverse osmosis (RO) membrane technology is increasingly used for large-scale production of potable water. However, the acidic and unbuffered RO permeate can damage water piping networks. Remineralization using an ion exchange resin - bipolar membrane electrodialysis hybrid process can protect the water distribution network. This study investigates the use of preparative ion exchange chromatography principles for monovalent-divalent ion separation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Tomas Molnar, Maria Bartosova, Monika Antosova, Ludovit Skultety, Milan Polakovic
Summary: This study successfully purified rhEPO using a three-step chromatographic procedure, with a purification factor of about 40. The process involved direct chromatographic separation of the protein from the post-harvest medium, with the removal of over 99% of impurity proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yin Yin Siew, Amrita Rai, Han Bin Pek, Dave Siak-Wei Ow, Wei Zhang
Summary: The new and efficient purification process for recombinant human insulin production significantly improved efficiency and achieved higher recovery and purity by exploring new resins and optimizing purification steps. The process is more economical and practical than conventional methods, especially when using Nuvia HR-S cation exchanger.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Sze Ying Lee, Bing-Lan Liu, Jun-Yi Wu, Yu-Kaung Chang
Summary: In this study, a weak ion-exchange membrane (P-COOH) was synthesized through alkaline hydrolysis and successfully used for the purification of lysozyme from chicken egg white with a high recovery yield of 98%. The scalability of the purification technique was demonstrated by achieving consistent results with different feedstock loading volumes and operating conditions.
Article
Biochemistry & Molecular Biology
Solomon Mengistu Lemma, Cristiana Boi, Ruben G. Carbonell
Summary: This study demonstrated the preparation and characterization of UV-grafted PBT ion exchange nonwoven membranes for chromatographic purification of biomolecules. Cation and anion exchange membranes achieved dynamic binding capacities of 200 mg hIgG/mL and 55 mg BSA/mL, respectively, showing significant potential for downstream purification of biologics.
Article
Pharmacology & Pharmacy
Ruth Rieser, Johanna Koch, Greta Faccioli, Klaus Richter, Tim Menzen, Martin Biel, Gerhard Winter, Stylianos Michalakis
Summary: Recombinant adeno-associated virus (rAAV) vectors are a promising technology for gene therapy, showing potential in treating genetic disorders. Efficient production and purification methods are in high demand for rAAVs, and liquid chromatography-based strategies, including combinations of different chromatography techniques, offer promise for achieving highly pure rAAVs.
Article
Engineering, Chemical
Saxena Nikita, Anamika Tiwari, Deepak Sonawat, Hariprasad Kodamana, Anurag S. Rathore
Summary: This study introduces a novel approach based on reinforcement learning to separate charge variants in biopharmaceutical industry by optimizing flow rate, achieving good results in experimental validation. Compared to traditional trial and error methods, this approach shows higher efficiency in both optimization and computational aspects.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Virology
Sanda Ravlic, Marija Brgles, Lea Hirsl, Stipan Jonjic, Beata Halassy
Summary: There is a high demand for a prophylactic vaccine against HCMV infections, and HCMV shows promise as a vector for gene therapy. Understanding the biological and biophysical properties of HCMV and comparing it with MCMV could lead to better utilization of HCMV for therapeutic purposes.
Article
Biotechnology & Applied Microbiology
Chintan Savla, Andre F. Palmer
Summary: By binding heme to human serum albumin and stabilizing it with polyethylene glycol, the anti-inflammatory effects of heme can be realized. This study provides a method for the synthesis and purification of heme protein carriers.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Chemical
Liangzhi Qiao, Yicheng Du, Kaifeng Du
Summary: In this study, a high-performance anion exchanger is developed by grafting DEAE-dextran on macroporous cellulose microspheres. The DEAE-dextran grafted MCMs show a higher adsorption capacity and faster mass transfer rate compared to traditional non-grafted DEAE modified anion exchangers. The results demonstrate the potential of DEAE-dextran grafted MCMs as high-performance ion exchangers.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Thomas Fuchs, Andreas Jupke
Summary: This work investigated the effects of salt type selection on cation exchange chromatography (CEC) for protein purification. It was found that a constant cationic strength is more important than ion valence in determining protein retention in CEC. The selection of anions or cations can significantly impact the separation factor, with changes of up to 30% and 16% observed, respectively.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mikael Andersson Schonn, Katri Eskelin, Dennis H. Bamford, Minna M. Poranen, Cecilia Unoson, Hanna M. Oksanen
Summary: An efficient chromatography-based virus purification method was developed, which can purify viruses in a single flow, saving time and minimizing virus loss. The method showed a 3.5-fold increase in yield compared to conventional methods and has the potential for purifying various viruses and nanoparticles.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Computer Science, Interdisciplinary Applications
Federico Rischawy, Till Briskot, Nathalie Hopf, David Saleh, Gang Wang, Simon Kluters, Joey Studts, Juergen Hubbuch
Summary: Mechanistic modeling was used to describe the entire downstream process of a Fab fragment, with various models connected to predict online and offline data and accommodate input process parameter variations at large manufacturing scale.
COMPUTERS & CHEMICAL ENGINEERING
(2023)
Article
Biochemical Research Methods
Moritz K. F. Wolf, Aurelie Closet, Monika Bzowska, Jean-Marc Bielser, Jonathan Souquet, Herve Broly, Massimo Morbidelli
BIOTECHNOLOGY JOURNAL
(2019)
Article
Biochemical Research Methods
Moritz K. F. Wolf, Aurelie Closet, Monika Bzowska, Jean-Marc Bielser, Jonathan Souquet, Herve Broly, Massimo Morbidelli
BIOTECHNOLOGY JOURNAL
(2019)
Article
Chemistry, Physical
Marcel Lorenz, Carolina Paganini, Giuseppe Storti, Massimo Morbidelli
Article
Materials Science, Multidisciplinary
Tianjin Yang, Alberto Cingolani, Tommaso Casalini, Abdessalem Aribia, Antoine Klaue, Hua Wu, Stavros Stavrakis, Andrew de Mello, Massimo Morbidelli
ADVANCED MATERIALS TECHNOLOGIES
(2019)
Article
Engineering, Chemical
Antoine Klaue, Matthias Kruck, Pietro Binel, Nic Friederichs, Francesco Bertola, Hua Wu, Massimo Morbidelli
Review
Biochemical Research Methods
Harini Narayanan, Martin F. Luna, Moritz von Stosch, Mariano Nicolas Cruz Bournazou, Gianmarco Polotti, Massimo Morbidelli, Alessandro Butte, Michael Sokolov
BIOTECHNOLOGY JOURNAL
(2020)
Article
Biotechnology & Applied Microbiology
Sebastian Vogg, Felix Pfeifer, Nicole Ulmer, Massimo Morbidelli
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Engineering, Environmental
Jichang Lu, Stefano Caimi, Peer Erfle, Bin Wu, Alberto Cingolani, Yongming Luo, Andreas Dietzel, Peicheng Luo, Hua Wu, Massimo Morbidelli
CHEMICAL ENGINEERING JOURNAL
(2019)
Article
Biochemical Research Methods
Chiara De Luca, Simona Felletti, Marco Macis, Walter Cabri, Giulio Lievore, Tatiana Chenet, Luisa Pasti, Massimo Morbidelli, Alberto Cavazzini, Martina Catani, Antonio Ricci
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
Sebastian Vogg, Thomas Mueller-Spaeth, Massimo Morbidelli
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biotechnology & Applied Microbiology
Harini Narayanan, Lars Behle, Martin F. Luna, Michael Sokolov, Gonzalo Guillen-Gosalbez, Massimo Morbidelli, Alessandro Butte
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Philipp Zurcher, Michael Sokolov, David Bruhlmann, Raphael Ducommun, Matthieu Stettler, Jonathan Souquet, Martin Jordan, Herve Broly, Massimo Morbidelli, Alessandro Butte
BIOTECHNOLOGY PROGRESS
(2020)
Article
Biochemical Research Methods
Chiara De Luca, Simona Felletti, Giulio Lievore, Alessandro Buratti, Sebastian Vogg, Massimo Morbidelli, Alberto Cavazzini, Martina Catani, Marco Macis, Antonio Ricci, Walter Cabri
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biotechnology & Applied Microbiology
Ce Shi, Sebastian Vogg, Dong-Qiang Lin, Mattia Sponchioni, Massimo Morbidelli
Summary: A study developed a design procedure (DP) suitable for optimizing both batch frontal chromatography and Flow2 in terms of purity, yield, and productivity. Comparing the two operated at their optimal conditions showed that the Flow2 process demonstrated a more favorable Pareto front of yield and productivity at a specified purity, and exhibited improved robustness compared to the batch process.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Ismaele Fioretti, Thomas Mueller-Spaeth, Richard Weldon, Sebastian Vogg, Massimo Morbidelli, Mattia Sponchioni
Summary: Oligonucleotides (ONs) are a promising class of biotherapeutics in the biopharmaceutical industry due to their unique way of regulating gene expression and influencing protein synthesis. However, their production cost limits large-scale manufacturing, which can be alleviated through process intensification. In this study, an efficient and continuous chromatographic purification process for ONs was developed, improving productivity and buffer consumption compared to traditional batch processes.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)