Article
Biophysics
Dongfei Chen, Yanfang Wu, Richard D. Tilley, J. Justin Gooding
Summary: An ultra-sensitive and highly-selective electrochemical biosensor has been developed for rapid detection of DNA methylation in blood, providing a promising method for minimally invasive diagnosis of ovarian cancer.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biotechnology & Applied Microbiology
Xiongtao Yu, Silan Bai, Lishi Wang
Summary: In this work, gold nanoparticles@Ti3C2 MXenes nanocomposites were used to construct an electrochemical circulating tumor DNA biosensor via toehold-mediated DNA strand displacement reaction. The biosensor has a linear detection range of 10 fM-10 nM and a detection limit of 0.38 fM, and can efficiently distinguish single base mismatched DNA sequences. The biosensor has been successfully applied for the sensitive detection of KRAS gene G12D, showing great potential for clinical analysis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Franziska Beck, Carina Horn, Antje J. Baeumner
Summary: Electrochemical immunosensors offer rapid analyte quantification with high sensitivity and selectivity, but user-friendliness can often be a challenge. Using silver nanoparticles instead of gold nanoparticles in POC sensors not only improves sensitivity, but also simplifies assay protocols to meet POC requirements, achieving lower limits of detection in a 2-minute automatic measurement.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jianjian Zhuang, Haitao Wan, Xiaobo Zhang
Summary: A biosensor using AuNP-modified AuE and CPs was developed for the detection of miR-100 in GC patients' sera. The biosensor showed high specificity and sensitivity in distinguishing miR-100 variants, with a detection range from 100 aM to 10 pM and a LOD of 100 aM. The cutoff for gastric cancer detection was determined to be 5 fM based on testing 100 human sera samples.
Article
Chemistry, Analytical
Yue He, Jing Zhou, Ruijie Fu, Yanlin Liu, Yiwen Wang, Haoran Liu, Jing Zhao, Yongliang Cui, Bining Jiao
Summary: An ultrasensitive colorimetric method for detecting genomic DNA of Alternaria from citrus fruit samples was described in this work, utilizing a system of five groups of reporter probes that form a DNA-heptamer sandwich structure on magnetic beads. The method has a low detection limit and is suitable for Alternaria genetic detection with its high sensitivity.
Article
Chemistry, Multidisciplinary
Xiangyuan Ouyang, Yongli Wu, Linjie Guo, Le Li, Mo Zhou, Xinyi Li, Ting Liu, Yawen Ding, Huaiyu Bu, Gang Xie, Jianlei Shen, Chunhai Fan, Lihua Wang
Summary: In this study, the ordered self-assembly of CuNCs using DNA nanoribbon as the template (DNR/CuNCs) was found to improve the electrochemiluminescence (ECL) properties of CuNCs compared with individual CuNCs. The DNR/CuNCs showed a high ECL yield of 46.8% in K2S2O8, which was approximately 68 times higher than that of individual CuNCs. This strategy was also successfully applied to other ECL emitters, such as gold nanoclusters and the Ru(bpy)(3)(2+)/TPrA system. Furthermore, a DNR/CuNC-based ECL biosensor with higher sensitivity was constructed for dopamine determination, showing the potential of DNR/CuNCs as a new type of superior luminophore candidate in ECL bioanalysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Tao Chen, Sha Yang, Qinzhen Li, Yongbo Song, Guang Li, Jinsong Chai, Manzhou Zhu
Summary: Hierarchical assemblies of a new silver nanocluster, Ag-70-TPP, have been synthesized and characterized, showing a highly space-efficient double helical 4H close packing pattern directed by ligands. The chirality of Ag-70-TPP and van der Waals forces interactions between ligands play a key role in driving its arrangement, with observed interlocking of phosphine ligands contributing to the structure. This work provides important and unprecedented insights into the internal structure and crystallographic arrangement of nanoclusters.
NANOSCALE HORIZONS
(2021)
Article
Chemistry, Analytical
Karen Y. P. S. Avelino, Leony S. Oliveira, Norma Lucena-Silva, Cesar A. S. Andrade, Maria D. L. Oliveira
Summary: A biosensor composed of PPy films and AuNPs was developed for specific detection of HPV genotypes, with modified oligonucleotides designed for strict recognition. The biosensor exhibited high sensitivity and specificity for HPV detection, as well as monitoring of the p53 gene biomarker. The flexible system showed a linear range for HPV detection with high sensitivity and accuracy.
Article
Chemistry, Analytical
Weizhong Li, Ying Cai, Yazhi Yang, Jinfeng Miao, Yuanyuan Xu
Summary: This paper proposes an electrochemical biosensor for NF-κB based on a PNA displacement reaction, which is more suitable for detecting DNA binding proteins. The study shows that the portable SPCE has better analysis performance and is suitable for determining NF-κB in different samples. This sensor holds promising prospect in studying NF-κB-associated signal pathways and diagnosing inflammatory related diseases due to its commercialized mass production.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Fan Yang, Shunsheng Ye, Wenhao Dong, Di Zheng, Yifan Xia, Chenglin Yi, Jing Tao, Chang Sun, Lei Zhang, Lu Wang, QianYun Chen, Yazi Wang, Zhihong Nie
Summary: This study presents a facile laser-scanning-based method to fabricate quasi-3D patterned arrays of plasmonic nanoparticle dimers with controlled orientation. The approach is highly flexible in forming high-resolution patterns of plasmonic dimers of different sizes and shapes.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Jue Yin, Mo Xie, Junke Wang, Meirong Cui, Dan Zhu, Shao Su, Chunhai Fan, Jie Chao, Qian Li, Lianhui Wang
Summary: In this work, the authors report a simple and versatile one-pot strategy to fabricate DNA architectures with the assistance of spherical gold nanoparticles modified with thiolated oligonucleotide strands. The desired architectures are formed via the stochastic co-assembly of different modules.
Article
Chemistry, Analytical
Sinuo Feng, Yu Xue, Jianshe Huang, Xiurong Yang
Summary: In this study, an electrochemical biosensor was successfully developed for the sensitive and specific determination of miR-21. The biosensor utilized COFs and DTN as the biosensing interface and employed a CHA strategy. The biosensor showed a low detection limit of 0.33 fM and a wide linear response ranging from 1 fM to 10 nM miR-21.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mansi Chaturvedi, Monika Patel, Neha Bisht, Maumita Das Mukherjee, Archana Tiwari, D. P. Mondal, Avanish Kumar Srivastava, Neeraj Dwivedi, Chetna Dhand
Summary: Tuberculosis (TB) is a devastating human illness and early diagnosis is crucial. This study proposes an electrochemical biosensor based on a ternary nanocomposite for highly sensitive and selective MTB detection. The sensor showed high sensitivity and stability, and provides a unique strategy for early MTB diagnosis. The electrochemical platform has potential for detecting various infectious pathogens and biomarkers.
Article
Chemistry, Physical
Shuqian Yu, Jie Zhang, Shenghua Liu, Ziyi Ma, Haotian Sun, Zhiwei Liu, Longgang Wang
Summary: In this study, gold nanoflowers with good photothermal conversion efficiency were successfully synthesized using polymyxin E as a template. The flower-shaped gold nanoparticles exhibited anisotropic properties and a large surface plasmon resonance peak, showing excellent antibacterial effects on HeLa cells and strong inhibition efficacy on tumor growth in vivo.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Analytical
Caroline G. Sanz, Daniel N. Crisan, Ricardo J. B. Leote, Melania Onea, Madalina M. Barsan
Summary: In this study, a biosensor for quantifying superoxide in cell culture media was successfully developed using gold nanoparticles and superoxide dismutase bioconjugates. The optimized biosensor achieved a sensitivity value of 41.2 nAμMcm(-2), a detection limit of 1.0μM, and showed excellent operational stability, indicating great potential for practical applications.
Article
Chemistry, Applied
Nafiseh Kazemifard, Ali A. Ensafi, Behzad Rezaei
Article
Chemistry, Multidisciplinary
Maryam Zakery, Ali A. Ensafi, Behzad Rezaei
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2020)
Article
Food Science & Technology
Muhannad Haj Mustafa, Sabihe Soleimanian-Zad, Mahmoud Sheikh-Zeinoddin
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Nanoscience & Nanotechnology
Farshad Tavakoli, Behzad Rezaei, Ahmad Reza Taghipour Jahromi, Ali A. Ensafi
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Biochemical Research Methods
N. Salandari-Jolge, Ali A. Ensafi, Behzad Rezaei
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Aram Mahmoodi, Ali A. Ensafi, Behzad Rezaei
Article
Environmental Sciences
Davood Khayyami, Ali Asghar Ensafi, Nafiseh Kazemifard, Behzad Rezaei
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Vahideh Saidi, Mahmoud Sheikh-Zeinoddin, Farzad Kobarfard, Sabihe Soleimanian-Zad
Article
Biotechnology & Applied Microbiology
Mehri Karim, Milad Fathi, Sabihe Soleimanian-Zad
FOOD AND BIOPRODUCTS PROCESSING
(2020)
Article
Spectroscopy
Sudabe Mandani, Behzad Rezaei, Ali Asghar Ensafi
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2020)
Article
Agriculture, Multidisciplinary
Niloufar Nourmohammadi, Sabihe Soleimanian-Zad, Hajar Shekarchizadeh
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2020)
Article
Immunology
Fariba Ghiamati Yazdi, Amin Zakeri, Ingrid van Ark, Thea Leusink-Muis, Saskia Braber, Sabihe Soleimanian-Zad, Gert Folkerts
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Engineering, Chemical
Mehri Karim, Milad Fathi, Sabihe Soleimanian-Zad
Summary: In this study, cinnamic aldehyde was successfully encapsulated in zein nanofibers in different concentrations, with the 40% concentration showing the best results. Release kinetics were studied in saliva, gastric, intestinal, and food simulated buffer media. The encapsulated cinnamic aldehyde exhibited bactericidal effects against bacteria and did not adversely affect the characteristics of sausages.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Vahideh Saidi, Mahmoud Sheikh-Zeinoddin, Farzad Kobarfard, Sabihe Soleimanian-Zad, Ali Sedaghat Doost
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2020)
Article
Engineering, Environmental
Reyhaneh Fazel Zarandi, Behzad Rezaei, Hassan S. Ghaziaskar, Ali Asghar Ensafi
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2019)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)