Article
Chemistry, Multidisciplinary
Zhenzhen Li, Jianbang Wang, Itamar Willner
Summary: Emulating native transient transcription machineries to modulate temporal gene expression using synthetic circuits is a challenging task in systems chemistry. Three different methods have been introduced to operate transient transcription machineries and modulate the gated transcription processes of target RNAs. These methods involve the design of reaction modules, the application of common fuel promoter strands, and the assembly of reaction modules triggered by fuel promoter strands.
Article
Spectroscopy
Yao Chen, Jin Yan, Xiaozhi Wang, Siwei Zhang, Jun Li, Ying Tang, Tong Wang
Summary: A fluorescence sensing method was developed for the quantification of alkaline phosphatase (ALP) by integrating strand displacement amplification with DNAzyme-catalytic recycling cleavage of molecular beacons. The proposed method achieved sensitive and specific ALP detection in human serum samples, showing potential for application in biomedical research and clinical diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Liang Tian, Jiyuan Zhang, Yanci Zhang, Huiling Fan, Chun Liu, Yihong Wang, Jiansheng Cui
Summary: A dual-model photoelectrochemical biosensor based on label-free trimetallic nanoprobe and Mg2+ dependent DNAzyme driven DNA walker was developed for ultrasensitive detection of microRNA-155 in serum at the early stage of cancer. The biosensor exhibited a low limit of detection (1.2 fM), excellent stability, and superior performance in complex human serum samples and tumor cell lysates, showing great potential for early-stage clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Chemical
Xuepeng Deng, Yang Han, Li-Chiang Lin, W. S. Winston Ho
Summary: A new measurement method for the steric hindrance effect of amine molecules, N exposure, is proposed. This method is independent of group-specific parameters and takes into account the spatial arrangements of pendant groups. It can effectively screen for sterically hindered amines with improved carbon dioxide loading capacities.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yunlong Qin, Yu Ouyang, Jianbang Wang, Xinghua Chen, Yang Sung Sohn, Itamar Willner
Summary: The article reports a functional bioreactor hybrid composed of glucose oxidase-loaded ZIF-90 metal-organic framework nanoparticles conjugated to hemin-G-quadruplexes, which can perform transient dissipative biocatalytic cascaded transformations. One system involves the temporal formation and depletion of the bioreactor conjugate operating the nickase-guided transient biocatalytic cascades, while the other demonstrates the transient operation of the two biocatalytic cascades dictated by exonuclease III.
Article
Biophysics
Lok Ting Chu, Hoi Kwan Kwong, Hogi Hartanto, Ting-Hsuan Chen
Summary: This study reported a method for detecting intracellular sodium ions by activating DNAzyme through endogenous mRNA, successfully achieving the detection of sodium ions in different phenotypic cells and observing an increase in their levels by increasing the permeability of the cell membrane.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ruonan Liu, Xiao Liu, Han Yang, Zongfen Chen, Zehua Xu, Hu Zuo, Xinyang Wang, Wen Wang, Lu Geng, Jinli Xie, Lingchao Cai, Yumei Jiang, Tieqiang Sun
Summary: A universal biosensing platform based on strand displacement amplification (SDA) and hybridization chain reaction (HCR) was developed for rapid detection of tetracycline (TC). The biosensor showed a linear relationship from 20 to 1000 ng/mL with a detection limit of 17.16 ng/mL. It exhibited good performance in TC analysis of different matrices, highlighting its potential in other fields.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Nan Jiang, Peng Li, Shihao Sun, Wei Wei
Summary: A novel ratiometric fluorescence sensor based on strand displacement reaction and magnetic separation was developed for highly sensitive and selective detection of HMF. The sensor has a wide linear range and high selectivity against other interfering substances, making it a potential tool for food safety and environmental monitoring.
Article
Chemistry, Applied
Bing Han, Cheng Fang, Lingjun Sha, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: A new fluorescent aptasensor using cascade strand displacement reaction was proposed for the detection of ochratoxin A, achieving an improved detection limit and short assay time. The method showed potential for use in food quality control in the future.
Article
Chemistry, Multidisciplinary
Na Wang, Yongjian Jiang, Kunhan Nie, Di Li, Hui Liu, Jian Wang, Chengzhi Huang, Chunmei Li
Summary: A cascade DNAzyme amplifier was designed using concurrent amplification cycle principles of toehold-mediated strand displacement reaction (TSDR) and Zn2+-assisted DNAzyme cycle. Compared to a single DNAzyme amplifier, the cascade DNAzyme amplifier exhibited higher sensitivity by releasing more DNAzyme through TSDR during the DNAzyme cycle. This detection strategy showed promise in early cancer diagnosis due to its ability to distinguish single-base mismatched DNA strands.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Shengyong Ding, Ailing Kan, Xiaoting Liu, Chao Huang, Nan Zhang, Wei Jiang
Summary: A strand-elongation initiated DNAzyme walker has been developed to detect TdT activity, providing a specific and sensitive method for research and diagnosis related to leukemia.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Jun Wang, Xiaokang Zhang, Peijun Shi, Ben Cao, Bin Wang
Summary: Living organisms respond to stimuli and this ability plays a crucial role in nature. Inspired by this, the development of nanodevices that can process time-related information can advance molecular information processing systems. In this study, a DNA finite-state machine that responds to sequential stimuli signals was proposed and implemented using a programmable allosteric strategy of DNAzyme. This state machine is capable of reversible logic control and order detection, and can contribute to the development of dynamic nanotechnology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Piaopiao Chen, Wu Peng, Runlian Qu, Yaqin He, Tangyuheng Liu, Jin Huang, Binwu Ying
Summary: Researchers developed an IFN-gamma aptasensor using pyrophosphate-cerium coordination polymeric nanoparticles as signal reporters and a double-stranded DNA as a probe for tuberculosis diagnosis. The sensor showed high detection sensitivity and target protein specificity and was validated in clinical samples, providing a new sensitive tool for early TB diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Rui Zhang, Mingrui Zhang, Qiao Ding, Ruo Yuan, Yali Yuan
Summary: In this study, a novel label-free and signal-off electrochemical biosensor based on efficient target-trigger T-structure recycling with dual strand displacement amplification (T-DSDA) was constructed for the sensitive detection of miRNA-155 related to cardiovascular diseases. The sensor demonstrated rapid and sensitive detection capability with a low detection limit, and showed strong anti-interference ability in bovine whole blood. This research provides a foundation for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Qiao Xu, Ke Liu, Jingjing Jin, Xinfeng Zhang
Summary: A cascaded strand displacement reaction system was designed for detecting low abundant biomarker protein in serum. The system converted a protein recognition event into DNA catalyst output, improving CHA reaction efficiency and CL signal.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)